FastQCFastQC Report
Fri 27 May 2016
SRR2139586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6080230
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC93500.054.6815031
GGTATCA111550.053.6037331
TATACAC115550.046.0198443
TTATACA122700.042.282742
GTATCAA207800.038.387151
TACACAT158700.033.3744625
ACACATC159000.031.8368326
ACATCTC161200.031.5573988
ATCAACG260400.030.1328333
TCAACGC261400.030.0488534
TATCAAC262600.029.9937292
CACATCT169050.029.8946027
CAACGCA267100.029.4389535
ATACACA188200.029.3444424
GTGGTAT46750.029.1992951
TGGTATC45700.028.8622862
AACGCAG275300.028.5012576
CATCTCC184150.027.4653519
ACGCAGA312100.025.167487
CGCAGAG315700.024.8931398
Produced by FastQC (version 0.11.5)