##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6080230
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.884952049511284	33.0	31.0	33.0	25.0	34.0
2	32.143131756528945	33.0	32.0	33.0	29.0	34.0
3	32.13223282671873	33.0	31.0	33.0	30.0	34.0
4	32.82989640194532	33.0	33.0	34.0	31.0	34.0
5	32.938123228891016	33.0	33.0	34.0	31.0	34.0
6	36.576812225853296	38.0	37.0	38.0	34.0	38.0
7	37.070848964595086	38.0	38.0	38.0	36.0	38.0
8	36.97577509403427	38.0	38.0	38.0	35.0	38.0
9	37.152945365553606	38.0	38.0	38.0	36.0	38.0
10-11	37.28546707937035	38.0	38.0	38.0	36.5	38.0
12-13	37.29475809303267	38.0	38.0	38.0	37.0	38.0
14-15	37.377535635987456	38.0	38.0	38.0	37.0	38.0
16-17	37.392027357517726	38.0	38.0	38.0	37.0	38.0
18-19	37.4087750792322	38.0	38.0	38.0	37.0	38.0
20-21	37.424168998370284	38.0	38.0	38.0	37.0	38.0
22-23	37.436633270982504	38.0	38.0	38.0	37.0	38.0
24-25	37.444939546534385	38.0	38.0	38.0	37.0	38.0
26-27	37.4181873108872	38.0	38.0	38.0	37.0	38.0
28-29	37.396217838306896	38.0	38.0	38.0	37.0	38.0
30-31	37.39082127582705	38.0	38.0	38.0	37.0	38.0
32-33	37.380161563486496	38.0	38.0	38.0	37.0	38.0
34-35	37.34553974592609	38.0	38.0	38.0	37.0	38.0
36-37	37.324700705994765	38.0	38.0	38.0	37.0	38.0
38-39	37.27449951161421	38.0	38.0	38.0	37.0	38.0
40-41	37.30319166447366	38.0	38.0	38.0	37.0	38.0
42-43	37.27238752140718	38.0	38.0	38.0	37.0	38.0
44-45	37.21897710766906	38.0	38.0	38.0	37.0	38.0
46-47	37.159699203935205	38.0	38.0	38.0	37.0	38.0
48-49	37.09062744917807	38.0	38.0	38.0	36.0	38.0
50-51	37.010542057993845	38.0	38.0	38.0	36.0	38.0
52-53	37.0291077778365	38.0	38.0	38.0	36.0	38.0
54-55	37.00432965907443	38.0	38.0	38.0	36.0	38.0
56-57	36.951838881413124	38.0	38.0	38.0	36.0	38.0
58-59	36.93095773060472	38.0	38.0	38.0	36.0	38.0
60-61	36.940719215726745	38.0	38.0	38.0	36.0	38.0
62-63	36.94026507495512	38.0	38.0	38.0	36.0	38.0
64-65	36.887667735144746	38.0	38.0	38.0	36.0	38.0
66-67	36.92208975108305	38.0	38.0	38.0	36.0	38.0
68-69	36.92942822925653	38.0	38.0	38.0	36.0	38.0
70-71	36.91316252399348	38.0	38.0	38.0	35.5	38.0
72-73	36.90806168005625	38.0	38.0	38.0	36.0	38.0
74-75	36.87224997536046	38.0	38.0	38.0	35.5	38.0
76-77	36.8819315344403	38.0	38.0	38.0	35.0	38.0
78-79	36.88702723810432	38.0	38.0	38.0	35.0	38.0
80-81	36.88840036290324	38.0	38.0	38.0	35.0	38.0
82-83	36.83968678569408	38.0	38.0	38.0	35.0	38.0
84-85	36.82565653671486	38.0	38.0	38.0	35.0	38.0
86-87	36.7973911231316	38.0	38.0	38.0	35.0	38.0
88-89	36.810181016234154	38.0	38.0	38.0	35.0	38.0
90-91	36.79780884390752	38.0	38.0	38.0	35.0	38.0
92-93	36.77977364611499	38.0	38.0	38.0	35.0	38.0
94-95	36.763689448680715	38.0	38.0	38.0	35.0	38.0
96-97	36.7487504293081	38.0	38.0	38.0	35.0	38.0
98-99	36.78796770281301	38.0	38.0	38.0	35.0	38.0
100	35.23567104212744	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	25.0
21	88.0
22	289.0
23	1001.0
24	2570.0
25	5138.0
26	9641.0
27	16703.0
28	26927.0
29	40347.0
30	53779.0
31	69350.0
32	88226.0
33	115326.0
34	168942.0
35	295009.0
36	810161.0
37	4376701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85555974033877	20.28054859766818	14.355591811493973	24.50829985049908
2	14.054206528237742	20.225711134814006	43.72656106725527	21.99352126969298
3	17.72011017453292	26.517938393509	30.913637132903006	24.848314299055076
4	12.009474663400116	18.52621683177045	38.58389241434843	30.880416090480995
5	12.93727375444679	37.41996602102223	35.793349922618056	13.84941030191292
6	29.11583936791865	37.980553367224594	18.669408887492743	14.234198377364013
7	25.884559534736923	33.21852848594933	22.32288456265965	18.574027416654097
8	24.150402205179738	36.80232491205103	21.115796606378375	17.93147627639086
9	25.300062662103244	16.94095453625932	21.086143122875285	36.67283967876215
10-11	23.800818390093795	27.030120571096816	28.67770627097988	20.491354767829506
12-13	24.94028186433737	24.450251059581625	28.841877692126776	21.767589383954224
14-15	22.10082513325976	25.733286405284012	26.595720885558606	25.570167575897624
16-17	21.39567911082311	29.422209357211816	27.20257292898459	21.97953860298048
18-19	21.447987987296532	27.691789619800566	29.578782381587537	21.28144001131536
20-21	22.312669456645004	26.641014655198642	29.517441750498964	21.52887413765739
22-23	21.863130238218268	27.171079019197713	29.44606812014266	21.519722622441353
24-25	21.867021745596006	27.209795519807127	29.683309360186765	21.239873374410102
26-27	21.81833602513714	27.26180415541677	29.66346680254428	21.25639301690181
28-29	21.38863503002147	26.88103247481159	29.896867588723776	21.833464906443165
30-31	21.57223904034357	27.048627840505134	30.017421696132153	21.36171142301914
32-33	20.914087076821946	26.778781199596075	30.51553275812927	21.791598965452707
34-35	21.08599371536907	26.881672525530277	30.057189875963324	21.975143883137324
36-37	21.23477992300657	27.102893824182555	30.26216255339076	21.400163699420112
38-39	21.18624711203583	26.815125854880655	30.239638337799423	21.75898869528409
40-41	21.405630443796618	26.777736410232006	30.227795372391313	21.588837773580057
42-43	21.764149966727146	26.682950464373295	30.068280827488213	21.48461874141134
44-45	21.29971015012163	27.295805051058856	29.859387831267465	21.54509696755205
46-47	21.928546305956516	26.80289602654302	29.118145946540118	22.150411720960342
48-49	21.78013808782308	26.86317524509642	29.074191717812344	22.282494949268155
50-51	21.39553283102979	27.152559896843364	29.382454373264917	22.06945289886193
52-53	21.53660038684331	27.256121857705267	29.740415709027534	21.466862046423888
54-55	21.52945791453213	27.355040823309494	29.05049094790631	22.065010314252063
56-57	22.01415589194263	27.765434387020843	28.36197411874916	21.85843560228737
58-59	21.246421661945327	28.241146778940397	29.254727710791844	21.257703848322436
60-61	21.81867305372107	28.867047101300958	28.139116043976003	21.175163801001972
62-63	21.187520307961247	29.393599813876186	28.1499210044973	21.268958873665262
64-65	21.21660847722143	29.21553526219619	28.207316964335728	21.360539296246653
66-67	21.349155774441307	29.027662840330983	28.141867570916002	21.481313814311704
68-69	21.449450761398463	28.65619060140289	28.44123364785829	21.453124989340353
70-71	21.510547697117225	28.07264091863307	28.671986994768194	21.74482438948151
72-73	21.731752485787055	27.63857450779167	28.764197218873356	21.865475787547922
74-75	21.660882505818055	27.753427994823337	28.684225082420735	21.901464416937877
76-77	21.744863846147368	27.65920695718326	28.661103291587136	21.93482590508223
78-79	21.7480065166095	27.668321966828213	28.59732584925573	21.986345667306562
80-81	21.741754361524507	27.738926614100805	28.523826887644145	21.995492136730547
82-83	21.888032043568664	27.668450282220203	28.58921079094834	21.854306883262797
84-85	21.946698782844692	27.658562472588706	28.468871208806746	21.92586753575986
86-87	21.77136926965045	27.763489748314825	28.502624848508184	21.962516133526538
88-89	21.912600946981957	27.687338970213972	28.46132049234666	21.938739590457416
90-91	21.906971201834764	27.716302379519064	28.460602625202036	21.916123793444136
92-93	21.91889422042372	27.77284121745303	28.427936248878655	21.880328313244597
94-95	21.831759972474053	27.822893641454183	28.41819991567182	21.92714647039994
96-97	21.971486724281313	27.914271799607633	28.421765378836955	21.692476097274103
98-99	22.98037552538778	29.81311228110443	28.687492889278975	18.519019304228806
100	21.924487312036227	30.737145175432858	22.66325529046865	24.675112222062268
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2848.0
1	2321.0
2	2154.0
3	2694.0
4	3214.0
5	4299.0
6	5578.0
7	7769.0
8	10557.0
9	11865.0
10	11297.5
11	10590.0
12	11581.5
13	14853.5
14	21260.5
15	28414.0
16	33592.0
17	36398.5
18	35893.5
19	32626.5
20	28885.0
21	25976.5
22	25346.0
23	28089.5
24	34005.0
25	42820.0
26	54164.5
27	66696.5
28	79062.0
29	92411.0
30	106119.0
31	120078.0
32	135709.0
33	151248.5
34	166383.0
35	180130.0
36	195110.0
37	207955.0
38	215071.5
39	221722.0
40	229690.0
41	235800.0
42	237892.5
43	241448.5
44	258670.0
45	286981.0
46	285822.5
47	270695.5
48	262627.5
49	247782.5
50	237284.0
51	228646.0
52	218261.0
53	205648.5
54	191863.0
55	178364.0
56	163023.5
57	146039.0
58	130632.0
59	114538.5
60	97010.0
61	79292.5
62	62520.0
63	48779.0
64	37525.5
65	28451.5
66	21325.0
67	16303.5
68	12669.0
69	9620.0
70	7166.5
71	5202.0
72	3762.0
73	2727.0
74	1896.0
75	1307.0
76	864.0
77	544.5
78	362.5
79	239.5
80	146.0
81	88.5
82	66.0
83	54.0
84	44.5
85	34.0
86	23.5
87	16.0
88	12.0
89	9.5
90	5.5
91	2.0
92	1.0
93	1.5
94	2.0
95	2.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00550965999641461
3	9.703580292192894E-4
4	2.302544476113568E-4
5	0.0
6	0.0
7	9.374645367033812E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.072719148819632E-4
24-25	6.250964375754022E-4
26-27	1.0694263350348182E-4
28-29	0.0028396267693344013
30-31	0.0022090898607473827
32-33	0.005091797841077716
34-35	0.010435685731563967
36-37	0.0014758745786774146
38-39	0.0
40-41	1.1007499494078388E-4
42-43	6.571740015819971E-4
44-45	2.4122509264766596E-4
46-47	4.355082411659984E-5
48-49	0.004481511958241113
50-51	0.0036631997267181876
52-53	3.766717161602839E-4
54-55	0.002821901902187996
56-57	0.04137133046201272
58-59	0.06418612354594434
60-61	0.030869459390105426
62-63	0.01107712393516566
64-65	9.873784551362762E-4
66-67	2.114524166704472E-4
68-69	0.001374161858724466
70-71	0.011371553894633499
72-73	0.017206856298298063
74-75	0.010878434611559566
76-77	0.013071147807984941
78-79	0.0025524568798206805
80-81	0.01463173496400436
82-83	0.04529633161197379
84-85	0.02533380174811798
86-87	0.0038290645032956995
88-89	1.2025292031483965E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	74.0
20-21	325.0
22-23	651.0
24-25	787.0
26-27	2408.0
28-29	6251.0
30-31	16143.0
32-33	20072.0
34-35	27336.0
36-37	41919.0
38-39	47916.0
40-41	45657.0
42-43	52029.0
44-45	62092.0
46-47	62947.0
48-49	57460.0
50-51	49560.0
52-53	46352.0
54-55	49219.0
56-57	50402.0
58-59	51167.0
60-61	53957.0
62-63	55016.0
64-65	62057.0
66-67	70038.0
68-69	70444.0
70-71	94490.0
72-73	53615.0
74-75	47985.0
76-77	50111.0
78-79	51705.0
80-81	51204.0
82-83	47543.0
84-85	46934.0
86-87	48328.0
88-89	49888.0
90-91	50931.0
92-93	49862.0
94-95	57191.0
96-97	173347.0
98-99	384267.0
100-101	3820550.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.51151309255062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.78528098342188	81.24054099467584
2	6.1160547033566655	10.826825119018185
3	1.245855044170619	3.308175451605839
4	0.41189677485302356	1.4583042712073104
5	0.18269923980904088	0.8085493078178482
6	0.08981015696811125	0.4769539730596203
7	0.04934399966893372	0.30572584509149475
8	0.028613406294086492	0.2026092708657205
9	0.021350803450123194	0.17008127272008486
>10	0.06686949955755783	0.9165451728104852
>50	0.0014928809938964801	0.08623645063881341
>100	6.262343904776041E-4	0.11479536497385952
>500	7.970480202446673E-5	0.05439244953750389
>1k	2.6568263482058647E-5	0.030265055977366374
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6446746257954057E-5	1.6446746257954057E-5
2	0.0	0.0	0.0	1.6446746257954057E-5	1.6446746257954057E-5
3	0.0	0.0	0.0	3.2893492515908114E-5	1.6446746257954057E-5
4	0.0	8.223373128977029E-5	0.0	3.2893492515908114E-5	1.6446746257954057E-5
5	0.0	8.223373128977029E-5	0.0	3.2893492515908114E-5	1.6446746257954057E-5
6	6.578698503181623E-5	8.223373128977029E-5	0.0	6.578698503181623E-5	1.6446746257954057E-5
7	6.578698503181623E-5	8.223373128977029E-5	0.0	6.578698503181623E-5	1.6446746257954057E-5
8	6.578698503181623E-5	9.868047754772434E-5	0.0	6.578698503181623E-5	1.6446746257954057E-5
9	6.578698503181623E-5	2.1380770135340276E-4	0.0	8.223373128977029E-5	1.6446746257954057E-5
10-11	9.868047754772434E-5	2.1380770135340276E-4	0.0	1.0690385067670138E-4	1.6446746257954057E-5
12-13	1.151272238056784E-4	2.1380770135340276E-4	0.0	1.3157397006363246E-4	2.4670119386931086E-5
14-15	1.151272238056784E-4	2.9604143264317306E-4	0.0	1.3157397006363246E-4	4.111686564488514E-5
16-17	1.3157397006363246E-4	2.9604143264317306E-4	0.0	1.3157397006363246E-4	4.934023877386217E-5
18-19	1.3157397006363246E-4	3.0426480577215007E-4	0.0	1.4802071632158653E-4	4.934023877386217E-5
20-21	1.3157397006363246E-4	3.124881789011271E-4	0.0	1.4802071632158653E-4	7.401035816079326E-5
22-23	1.726908357085176E-4	3.6182841767498923E-4	0.0	1.8091420883749462E-4	9.045710441874731E-5
24-25	1.8091420883749464E-4	4.1939202957782845E-4	0.0	2.1380770135340276E-4	9.868047754772434E-5
26-27	1.8091420883749464E-4	4.4406214896475953E-4	0.0	2.87818059514196E-4	9.868047754772434E-5
28-29	1.8091420883749464E-4	4.4406214896475953E-4	0.0	6.578698503181623E-4	1.151272238056784E-4
30-31	1.973609550954487E-4	5.262958802545298E-4	0.0	0.001981832924083464	1.3157397006363246E-4
32-33	1.973609550954487E-4	5.59189372770438E-4	0.0	0.004572195459711228	1.3157397006363246E-4
34-35	2.0558432822442572E-4	6.414231040602083E-4	0.0	0.007524386413013982	1.3157397006363246E-4
36-37	2.1380770135340276E-4	7.401035816079326E-4	0.0	0.012113028618983165	1.3157397006363246E-4
38-39	2.1380770135340276E-4	7.729970741238407E-4	0.0	0.018724620614680696	1.3157397006363246E-4
40-41	2.302544476113568E-4	8.71677551671565E-4	0.0	0.025286872371604364	1.3157397006363246E-4
42-43	2.302544476113568E-4	8.963476710584961E-4	0.0	0.031503742457110996	1.4802071632158653E-4
44-45	2.5492456699828785E-4	0.0010114748948641746	0.0	0.03874031081061078	1.6446746257954057E-4
46-47	2.631479401272649E-4	0.0011019319992829218	0.0	0.04660185552191282	1.6446746257954057E-4
48-49	2.631479401272649E-4	0.001118378745540876	0.0	0.05396999784547624	1.973609550954487E-4
50-51	2.631479401272649E-4	0.0013404098200232558	0.0	0.0608940780200749	1.973609550954487E-4
52-53	2.79594686385219E-4	0.0013486331931522328	0.0	0.0670862779861946	1.973609550954487E-4
54-55	2.79594686385219E-4	0.0013486331931522328	0.0	0.07333604156421714	1.973609550954487E-4
56-57	2.79594686385219E-4	0.0013486331931522328	0.0	0.08038347233575047	2.1380770135340276E-4
58-59	2.79594686385219E-4	0.0013486331931522328	0.0	0.08702795782396389	2.3847782074033384E-4
60-61	2.9604143264317306E-4	0.0013486331931522328	0.0	0.09303102020811713	2.4670119386931085E-4
62-63	3.2893492515908114E-4	0.0013486331931522328	0.0	0.09931367727865559	2.631479401272649E-4
64-65	3.2893492515908114E-4	0.0015788876407635895	0.0	0.1050289216032946	2.631479401272649E-4
66-67	3.2893492515908114E-4	0.0015871110138925666	0.0	0.11051391148032229	2.631479401272649E-4
68-69	3.2893492515908114E-4	0.0016364512526664287	0.0	0.11604001822299485	2.631479401272649E-4
70-71	3.3715829828805815E-4	0.001669344745182337	0.0	0.1213934341299589	2.71371313256242E-4
72-73	3.618284176749893E-4	0.0016775681183113138	0.0	0.12557090767947923	2.79594686385219E-4
74-75	3.618284176749893E-4	0.001694014864569268	0.0	0.12870401284161948	2.79594686385219E-4
76-77	3.618284176749893E-4	0.0017269083570851762	0.0	0.1319522452275654	2.79594686385219E-4
78-79	3.618284176749893E-4	0.00174335510334313	0.0	0.1342136728380341	2.79594686385219E-4
80-81	3.618284176749893E-4	0.0017515784764721072	0.0	0.13487154268835225	2.87818059514196E-4
82-83	3.618284176749893E-4	0.0017762485958590381	0.0	0.1354554021805096	2.9604143264317306E-4
84-85	3.618284176749893E-4	0.0018091420883749464	0.0	0.13577611373253973	2.9604143264317306E-4
86-87	3.618284176749893E-4	0.0018091420883749464	0.0	0.13593235782199029	3.0426480577215007E-4
88	3.618284176749893E-4	0.0018749290734067627	0.0	0.13593235782199029	3.124881789011271E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	9350	0.0	54.681503	1
GGTATCA	11155	0.0	53.603733	1
TATACAC	11555	0.0	46.019844	3
TTATACA	12270	0.0	42.28274	2
GTATCAA	20780	0.0	38.38715	1
TACACAT	15870	0.0	33.374462	5
ACACATC	15900	0.0	31.836832	6
ACATCTC	16120	0.0	31.557398	8
ATCAACG	26040	0.0	30.132833	3
TCAACGC	26140	0.0	30.048853	4
TATCAAC	26260	0.0	29.993729	2
CACATCT	16905	0.0	29.894602	7
CAACGCA	26710	0.0	29.438953	5
ATACACA	18820	0.0	29.344442	4
GTGGTAT	4675	0.0	29.199295	1
TGGTATC	4570	0.0	28.862286	2
AACGCAG	27530	0.0	28.501257	6
CATCTCC	18415	0.0	27.465351	9
ACGCAGA	31210	0.0	25.16748	7
CGCAGAG	31570	0.0	24.893139	8
>>END_MODULE