FastQCFastQC Report
Fri 27 May 2016
SRR2139585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5922416
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132400.052.9049841
GTATCAA262450.039.6162641
GTGGTAT56400.034.038191
TGGTATC58050.032.4292372
ATCAACG333500.030.7521343
TATCAAC335400.030.7159442
TCAACGC334600.030.7002354
CAACGCA337500.030.3751495
AACGCAG348550.029.4359116
ACGCAGA395300.025.944227
CGCAGAG401650.025.5649478
GCAGAGT443450.023.2018189
GTACATG347000.021.7124121
GAGTACT235550.021.19020312-13
TACATGG351700.021.0340422
ACATGGG350800.020.39063
GTACTTT252950.019.63139314-15
CAGAGTA439200.019.54078510-11
AGAGTAC422750.018.68645710-11
AGTACTT249550.018.1529612-13
Produced by FastQC (version 0.11.5)