##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139585_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5922416
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83489677185797	33.0	31.0	33.0	25.0	34.0
2	32.12111999562341	33.0	31.0	33.0	29.0	34.0
3	32.14877728953859	33.0	31.0	33.0	30.0	34.0
4	32.84232836734198	33.0	33.0	34.0	31.0	34.0
5	32.95585686652204	33.0	33.0	34.0	31.0	34.0
6	36.61935804577051	38.0	37.0	38.0	34.0	38.0
7	37.11129680859973	38.0	38.0	38.0	36.0	38.0
8	37.0226866873249	38.0	38.0	38.0	36.0	38.0
9	37.185006929604405	38.0	38.0	38.0	36.0	38.0
10-11	37.31052816958484	38.0	38.0	38.0	36.5	38.0
12-13	37.311947691617746	38.0	38.0	38.0	37.0	38.0
14-15	37.400041469562424	38.0	38.0	38.0	37.0	38.0
16-17	37.41991064119778	38.0	38.0	38.0	37.0	38.0
18-19	37.43393777134197	38.0	38.0	38.0	37.0	38.0
20-21	37.445176918702145	38.0	38.0	38.0	37.0	38.0
22-23	37.4572805380801	38.0	38.0	38.0	37.0	38.0
24-25	37.472073541612104	38.0	38.0	38.0	37.0	38.0
26-27	37.449957215857985	38.0	38.0	38.0	37.0	38.0
28-29	37.42447943117622	38.0	38.0	38.0	37.0	38.0
30-31	37.428386605286256	38.0	38.0	38.0	37.0	38.0
32-33	37.414781601153294	38.0	38.0	38.0	37.0	38.0
34-35	37.38544019474989	38.0	38.0	38.0	37.0	38.0
36-37	37.36879600797566	38.0	38.0	38.0	37.0	38.0
38-39	37.35071249951862	38.0	38.0	38.0	37.0	38.0
40-41	37.33654798851683	38.0	38.0	38.0	37.0	38.0
42-43	37.298517782850446	38.0	38.0	38.0	37.0	38.0
44-45	37.2487279665354	38.0	38.0	38.0	37.0	38.0
46-47	37.18607658545743	38.0	38.0	38.0	37.0	38.0
48-49	37.118502545741805	38.0	38.0	38.0	37.0	38.0
50-51	37.04707788809001	38.0	38.0	38.0	36.0	38.0
52-53	37.07369544608022	38.0	38.0	38.0	36.0	38.0
54-55	37.058508657447476	38.0	38.0	38.0	36.0	38.0
56-57	37.009802837586946	38.0	38.0	38.0	36.0	38.0
58-59	37.000821527640504	38.0	38.0	38.0	36.0	38.0
60-61	37.0270259840305	38.0	38.0	38.0	36.0	38.0
62-63	37.03275604212984	38.0	38.0	38.0	36.0	38.0
64-65	36.98327276945546	38.0	38.0	38.0	36.0	38.0
66-67	37.029380748453086	38.0	38.0	38.0	36.0	38.0
68-69	37.046267424926526	38.0	38.0	38.0	36.0	38.0
70-71	37.034498515271224	38.0	38.0	38.0	36.0	38.0
72-73	37.034103722122325	38.0	38.0	38.0	36.0	38.0
74-75	36.99444806406174	38.0	38.0	38.0	36.0	38.0
76-77	37.00207691662253	38.0	38.0	38.0	36.0	38.0
78-79	37.00460314290047	38.0	38.0	38.0	36.0	38.0
80-81	37.001656132286016	38.0	38.0	38.0	36.0	38.0
82-83	36.95109668198534	38.0	38.0	38.0	36.0	38.0
84-85	36.92994625263532	38.0	38.0	38.0	35.0	38.0
86-87	36.89963778536414	38.0	38.0	38.0	35.0	38.0
88-89	36.9069370358673	38.0	38.0	38.0	35.0	38.0
90-91	36.887692503982066	38.0	38.0	38.0	35.0	38.0
92-93	36.864237408816436	38.0	38.0	38.0	35.0	38.0
94-95	36.84353335108198	38.0	38.0	38.0	35.0	38.0
96-97	36.8227211366491	38.0	38.0	38.0	35.0	38.0
98-99	36.85923200662795	38.0	38.0	38.0	35.0	38.0
100	35.440530208282915	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	4.0
19	0.0
20	16.0
21	59.0
22	263.0
23	931.0
24	2295.0
25	4938.0
26	8632.0
27	14666.0
28	23799.0
29	35452.0
30	48382.0
31	62189.0
32	80298.0
33	105496.0
34	153901.0
35	271652.0
36	761174.0
37	4348266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.476533225629545	20.008219618480023	14.445354733608717	23.06989242228172
2	14.634911564736125	20.250648759420688	43.093093093093096	22.02134658275009
3	18.266289992693796	26.874627576864167	29.937931869424418	24.921150561017622
4	12.349294907091048	17.603692144972715	38.83239444206084	31.214618505875396
5	13.114377645879655	37.783516051557335	34.84479982493631	14.257306477626699
6	29.650618936596146	37.56701656891377	18.528401247058632	14.253963247431454
7	26.847738596144342	33.496320133136095	22.298032828461487	17.35790844225808
8	25.101445085924396	36.03103868421266	20.48496762132211	18.382548608540837
9	26.45028650469673	16.825380047602195	21.22871814475714	35.49561530294393
10-11	24.500153315808955	27.03736954648238	27.770972184324776	20.691504953383888
12-13	25.66748097398089	24.86348476702751	28.418984752168708	21.05004950682289
14-15	22.769145227218083	25.72143699463192	26.91938560209212	24.59003217605788
16-17	21.205526595902754	29.166728578336947	27.515291056893	22.112453768867297
18-19	21.22097637180502	27.65026131227526	30.322430913329967	20.806331402589752
20-21	22.67006590169804	26.385236128047236	30.231255510104816	20.71344246014991
22-23	22.393087061241623	26.390626553497505	30.088221974831153	21.12806441042972
24-25	21.797392703676806	26.682146353169216	30.250687270196998	21.26977367295698
26-27	21.534832266422196	26.76336177509309	30.29460744843554	21.40719851004917
28-29	21.358910240761354	26.69069492469663	30.445670641826695	21.504724192715326
30-31	21.627693849012275	26.663089328721263	30.24245234226668	21.466764479999785
32-33	21.439019861370245	26.860086604293333	30.188509443318207	21.512384091018212
34-35	21.620209133534807	26.886053016093165	30.22432510122735	21.269412749144674
36-37	21.59152974243583	26.740583440734273	30.023026064448455	21.644860752381437
38-39	21.500429575471543	26.797383021731903	30.170912078030064	21.53127532476649
40-41	21.649350349110748	26.70290887090688	30.13143069758028	21.516310082402086
42-43	21.613281659473394	26.841849419100345	29.806580363821737	21.73828855760452
44-45	21.66271193284821	27.026231101018173	29.572093590840794	21.738963375292826
46-47	21.87840385675715	27.105322837153278	29.08257915230671	21.93369415378286
48-49	21.859711955968606	27.26750499850984	28.811771860022027	22.061011185499524
50-51	21.920619684713145	27.51596372444632	28.42393480590037	22.13948178494017
52-53	22.029570631611026	27.623788598173167	28.160732120595743	22.185908649620064
54-55	22.065492999105434	27.64806958440827	28.0699778311862	22.216459585300093
56-57	22.131535090892566	27.685443034889655	27.928672554039935	22.254349320177845
58-59	21.969168597884305	27.67170559325779	28.12934306589089	22.229782742967018
60-61	22.084867186380976	27.757676815913907	28.0709192007076	22.086536796997514
62-63	22.0084309194485	27.794124480417388	28.03478521320633	22.162659386927782
64-65	21.99335665370504	27.730754041628092	28.02366875226644	22.252220552400434
66-67	22.101381383559602	27.6974354944652	27.903756492865405	22.297426629109797
68-69	22.06071061144738	27.629483852468816	28.056964220714747	22.25284131536906
70-71	22.044579628676132	27.388925302902845	28.21734842060364	22.349146647817385
72-73	22.138264072038073	27.543490755909588	28.005961664744838	22.3122835073075
74-75	22.043369877906464	27.690644704725564	27.94930206017796	22.316683357190012
76-77	22.162168913168305	27.54419766154322	27.963253096480955	22.33038032880752
78-79	22.129896601519928	27.48965750637028	27.88304535104223	22.497400541067563
80-81	22.079825148826604	27.684645230913258	27.803831133154798	22.43169848710534
82-83	22.29443718642738	27.566712428141482	27.8550152529027	22.28383513252844
84-85	22.236769724964937	27.656070632619468	27.710934221532774	22.396225420882825
86-87	22.153960619668908	27.74069385685153	27.818760243810463	22.2865852796691
88-89	22.254385840494177	27.599476668559348	27.782697577292957	22.363439913653515
90-91	22.315895254076036	27.634247079744583	27.72758035284796	22.322277313331426
92-93	22.300823477284958	27.724227959984805	27.708895612285634	22.26605295044461
94-95	22.13444846268234	27.690637170310158	27.81061783745068	22.364296529556825
96-97	22.35144170032777	27.808406237525098	27.707048387891863	22.133103674255274
98-99	23.308735734553586	29.694615685019254	27.99397960139316	19.002668979033995
100	29.828169273235872	40.738332325288305	29.433498401475823	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4808.0
1	3920.0
2	2778.0
3	2946.5
4	4168.0
5	5750.0
6	6735.5
7	6894.5
8	6666.0
9	6613.5
10	6832.0
11	8161.0
12	10850.0
13	17280.0
14	27802.0
15	39494.0
16	49155.5
17	52870.5
18	51587.0
19	45002.0
20	37163.0
21	31500.0
22	28167.5
23	29330.5
24	33784.5
25	40864.0
26	50287.0
27	61438.5
28	73544.0
29	87577.0
30	102742.5
31	116804.5
32	133015.0
33	151465.5
34	165215.5
35	178951.5
36	195668.5
37	207405.0
38	216166.5
39	220525.0
40	223198.5
41	224621.5
42	223623.0
43	227208.0
44	234209.5
45	240631.5
46	241191.5
47	239537.0
48	238862.0
49	237098.5
50	234173.0
51	229214.0
52	221700.5
53	211638.5
54	200038.5
55	187799.0
56	173022.5
57	156770.0
58	141380.5
59	123551.5
60	105138.0
61	87449.5
62	70508.5
63	55970.0
64	43290.0
65	32744.0
66	24248.0
67	18404.0
68	14272.5
69	10842.5
70	8002.5
71	5898.5
72	4466.5
73	3234.0
74	2276.5
75	1569.5
76	1038.5
77	668.0
78	468.0
79	331.0
80	228.0
81	154.0
82	115.5
83	99.5
84	73.5
85	48.5
86	33.5
87	27.5
88	22.5
89	17.0
90	12.5
91	7.0
92	2.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005808440339212915
3	8.94905052262455E-4
4	8.442500493042028E-5
5	0.0
6	0.0
7	9.286750542346232E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.688920730674398E-5
22-23	7.686915804957774E-4
24-25	5.492216684002356E-4
26-27	6.762048745242687E-5
28-29	0.0030789328418770055
30-31	0.0024740806163917667
32-33	0.005098609226868141
34-35	0.010098258442749612
36-37	0.0013463347537022061
38-39	0.0
40-41	1.1341124627793014E-4
42-43	8.537327042115427E-4
44-45	2.2235878016107848E-4
46-47	4.503198576917199E-5
48-49	0.0045778818997662726
50-51	0.0035703374514616786
52-53	3.840984011388799E-4
54-55	0.0026668822869366743
56-57	0.04358243820718166
58-59	0.0662424165418929
60-61	0.03167351120344311
62-63	0.01164988169599392
64-65	9.167825516350369E-4
66-67	1.3096530598079263E-4
68-69	0.0014168279200313782
70-71	0.011155169962068294
72-73	0.017682963576959587
74-75	0.01164540491643023
76-77	0.013896486120857804
78-79	0.003271825886168102
80-81	0.01463510657122843
82-83	0.047310575733313924
84-85	0.02628375743201203
86-87	0.00405919688506955
88-89	6.863058421327274E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	681.0
20-21	2212.0
22-23	1627.0
24-25	1930.0
26-27	3301.0
28-29	7458.0
30-31	16645.0
32-33	20031.0
34-35	27677.0
36-37	43948.0
38-39	53225.0
40-41	49568.0
42-43	56086.0
44-45	68376.0
46-47	70344.0
48-49	64190.0
50-51	85568.0
52-53	49460.0
54-55	52596.0
56-57	54851.0
58-59	55918.0
60-61	55158.0
62-63	50865.0
64-65	53655.0
66-67	57011.0
68-69	59132.0
70-71	60520.0
72-73	52214.0
74-75	50450.0
76-77	53463.0
78-79	55528.0
80-81	55502.0
82-83	50599.0
84-85	48402.0
86-87	49871.0
88-89	53237.0
90-91	55519.0
92-93	53579.0
94-95	59714.0
96-97	165297.0
98-99	1290449.0
100-101	2706559.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.99697130079532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.71633474936387	76.25616729904227
2	7.184550949301703	12.213301416937973
3	1.7520661359168452	4.467609452148584
4	0.6398443111059052	2.1753931419218313
5	0.283780691381286	1.2060249640527512
6	0.14650622001506294	0.7471550986804987
7	0.08796106559450746	0.5233496917546608
8	0.04935508173221239	0.3356025972433011
9	0.03177116514875717	0.2430407531107568
>10	0.10365702728004263	1.3734517064740042
>50	0.002738813120354022	0.15548748746070526
>100	0.001335124772587833	0.23372666794009136
>500	6.577685023691255E-5	0.03779402893841033
>1k	3.288841659452887E-5	0.03189569429413113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.377000197216811E-5	0.0	0.0	0.0
3	0.0	3.377000197216811E-5	0.0	0.0	0.0
4	0.0	8.442500493042028E-5	0.0	0.0	0.0
5	0.0	8.442500493042028E-5	0.0	0.0	0.0
6	0.0	8.442500493042028E-5	0.0	0.0	0.0
7	0.0	8.442500493042028E-5	0.0	0.0	0.0
8	0.0	8.442500493042028E-5	0.0	0.0	1.6885000986084056E-5
9	0.0	2.701600157773449E-4	0.0	0.0	1.6885000986084056E-5
10-11	0.0	2.701600157773449E-4	0.0	1.6885000986084056E-5	1.6885000986084056E-5
12-13	0.0	2.8704501676342896E-4	0.0	1.6885000986084056E-5	1.6885000986084056E-5
14-15	0.0	4.2212502465210146E-4	0.0	1.6885000986084056E-5	3.377000197216811E-5
16-17	0.0	4.2212502465210146E-4	0.0	1.6885000986084056E-5	3.377000197216811E-5
18-19	0.0	4.305675251451435E-4	0.0	3.377000197216811E-5	3.377000197216811E-5
20-21	0.0	4.390100256381855E-4	0.0	5.0655002958252176E-5	3.377000197216811E-5
22-23	0.0	4.727800276103536E-4	0.0	6.754000394433623E-5	5.065500295825217E-5
24-25	0.0	5.656475330338159E-4	0.0	8.442500493042028E-5	6.754000394433623E-5
26-27	0.0	6.247450364851101E-4	0.0	1.9417751133996665E-4	7.598250443737825E-5
28-29	0.0	6.416300374711941E-4	0.0	6.247450364851102E-4	8.442500493042028E-5
30-31	0.0	7.344975428946565E-4	0.0	0.001967102614878793	8.442500493042028E-5
32-33	0.0	7.429400433876986E-4	0.0	0.004153710242576678	8.442500493042028E-5
34-35	0.0	8.780200512763709E-4	0.0	0.006939735405280548	8.442500493042028E-5
36-37	0.0	0.0010215425596580853	0.0	0.011346720662648486	8.442500493042028E-5
38-39	0.0	0.0010553125616302537	0.0	0.018278013567435993	8.442500493042028E-5
40-41	0.0	0.00112285256557459	0.0	0.025901591512652943	8.442500493042028E-5
42-43	0.0	0.0011566225675467578	0.0	0.032723131911030906	8.442500493042028E-5
44-45	0.0	0.0013001450759284724	0.0	0.03949401730645061	8.442500493042028E-5
46-47	0.0	0.001418340082831061	0.0	0.04699940024476497	8.442500493042028E-5
48-49	0.0	0.001435225083817145	0.0	0.05493535070822448	1.0131000591650435E-4
50-51	0.0	0.001739155101566658	0.0	0.06242384864555277	1.0131000591650435E-4
52-53	0.0	0.001772925103538826	0.0	0.06983636407844365	1.181950069025884E-4
54-55	0.0	0.0017982526050179522	0.0	0.07756969453007015	1.519650088747565E-4
56-57	0.0	0.0018235801064970783	0.0	0.08555629999648792	1.6885000986084056E-4
58-59	0.0	0.0018404651074831625	0.0	0.093205205443184	1.6885000986084056E-4
60-61	0.0	0.0019248901124135826	0.0	0.10059239337459577	1.6885000986084056E-4
62-63	0.0	0.001983987615864877	0.0	0.10792892630304929	1.6885000986084056E-4
64-65	0.0	0.0022794751331213477	0.0	0.11426080167283081	1.6885000986084056E-4
66-67	0.0	0.0022963601341074317	0.0	0.12109078457170182	1.6885000986084056E-4
68-69	0.0	0.0022963601341074317	0.0	0.1274142174409903	1.6885000986084056E-4
70-71	0.0	0.0022963601341074317	0.0	0.13339995029055712	1.6885000986084056E-4
72-73	0.0	0.0022963601341074317	0.0	0.1383219280780006	2.026200118330087E-4
74-75	0.0	0.002321687635586558	0.0	0.14180868078162695	2.026200118330087E-4
76-77	0.0	0.0023723426385448103	0.0	0.1452954334852533	2.026200118330087E-4
78-79	0.0	0.002397670140023936	0.0	0.14781974113267288	2.026200118330087E-4
80-81	0.0	0.002397670140023936	0.0	0.14859645117803275	2.1950501281909276E-4
82-83	0.0	0.002397670140023936	0.0	0.14923808121550394	2.701600157773449E-4
84-85	0.0	0.002431440141996104	0.0	0.14937316122339261	2.701600157773449E-4
86-87	0.0	0.0024989801459404403	0.0	0.14942381622635087	2.701600157773449E-4
88	0.0	0.002600290151856945	0.0	0.1494322587268439	2.701600157773449E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13240	0.0	52.904984	1
GTATCAA	26245	0.0	39.616264	1
GTGGTAT	5640	0.0	34.03819	1
TGGTATC	5805	0.0	32.429237	2
ATCAACG	33350	0.0	30.752134	3
TATCAAC	33540	0.0	30.715944	2
TCAACGC	33460	0.0	30.700235	4
CAACGCA	33750	0.0	30.375149	5
AACGCAG	34855	0.0	29.435911	6
ACGCAGA	39530	0.0	25.94422	7
CGCAGAG	40165	0.0	25.564947	8
GCAGAGT	44345	0.0	23.201818	9
GTACATG	34700	0.0	21.712412	1
GAGTACT	23555	0.0	21.190203	12-13
TACATGG	35170	0.0	21.034042	2
ACATGGG	35080	0.0	20.3906	3
GTACTTT	25295	0.0	19.631393	14-15
CAGAGTA	43920	0.0	19.540785	10-11
AGAGTAC	42275	0.0	18.686457	10-11
AGTACTT	24955	0.0	18.15296	12-13
>>END_MODULE