FastQCFastQC Report
Fri 27 May 2016
SRR2139584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6238552
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100950.052.230091
GTATCAA185100.038.0184941
TCAACGC224700.030.9577584
ATCAACG225850.030.7823773
CAACGCA229850.030.2457125
TATCAAC232250.030.1544442
AACGCAG238800.029.0944146
GTGGTAT46100.028.2721481
TGGTATC46550.026.544362
ACGCAGA272250.025.47317
CGCAGAG277400.025.0455468
GCAGAGT313150.022.2133059
GTACATG254300.020.6340451
GAGTACT165300.020.54168112-13
TACATGG256300.020.1428182
ACATGGG253600.019.8889433
CAGAGTA310500.019.61615610-11
GTACTTT180650.018.69383214-15
AGAGTAC291600.018.17414710-11
AGTACTT178250.016.99602112-13
Produced by FastQC (version 0.11.5)