##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6238552
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.998082728171536	33.0	31.0	33.0	25.0	34.0
2	32.19383456289216	33.0	33.0	33.0	29.0	34.0
3	32.2014425783419	33.0	33.0	33.0	31.0	34.0
4	32.871241595806204	33.0	33.0	34.0	31.0	34.0
5	32.98979186195771	33.0	33.0	34.0	31.0	34.0
6	36.666237934700234	38.0	37.0	38.0	34.0	38.0
7	37.15428195517165	38.0	38.0	38.0	36.0	38.0
8	37.06035759580108	38.0	38.0	38.0	36.0	38.0
9	37.227283510660804	38.0	38.0	38.0	36.0	38.0
10-11	37.35186049583301	38.0	38.0	38.0	37.0	38.0
12-13	37.35400354120635	38.0	38.0	38.0	37.0	38.0
14-15	37.43024559224641	38.0	38.0	38.0	37.0	38.0
16-17	37.44957339459542	38.0	38.0	38.0	37.0	38.0
18-19	37.46050557885869	38.0	38.0	38.0	37.0	38.0
20-21	37.47162882398739	38.0	38.0	38.0	37.0	38.0
22-23	37.48514639196438	38.0	38.0	38.0	37.0	38.0
24-25	37.498983085674084	38.0	38.0	38.0	37.0	38.0
26-27	37.47592063831489	38.0	38.0	38.0	37.0	38.0
28-29	37.452624591866126	38.0	38.0	38.0	37.0	38.0
30-31	37.45846372441939	38.0	38.0	38.0	37.0	38.0
32-33	37.44705023995576	38.0	38.0	38.0	37.0	38.0
34-35	37.42341766946171	38.0	38.0	38.0	37.0	38.0
36-37	37.410393007289855	38.0	38.0	38.0	37.0	38.0
38-39	37.39942981120171	38.0	38.0	38.0	37.0	38.0
40-41	37.38687202703008	38.0	38.0	38.0	37.0	38.0
42-43	37.35699506617543	38.0	38.0	38.0	37.0	38.0
44-45	37.32419316005111	38.0	38.0	38.0	37.0	38.0
46-47	37.285050136467376	38.0	38.0	38.0	37.0	38.0
48-49	37.23336357784649	38.0	38.0	38.0	37.0	38.0
50-51	37.16214692892088	38.0	38.0	38.0	37.0	38.0
52-53	37.18801212154965	38.0	38.0	38.0	37.0	38.0
54-55	37.171883908198524	38.0	38.0	38.0	37.0	38.0
56-57	37.122188947419154	38.0	38.0	38.0	36.0	38.0
58-59	37.11112528232886	38.0	38.0	38.0	36.0	38.0
60-61	37.13050472881461	38.0	38.0	38.0	36.0	38.0
62-63	37.13131601020021	38.0	38.0	38.0	36.0	38.0
64-65	37.07680771484592	38.0	38.0	38.0	36.0	38.0
66-67	37.12121945670664	38.0	38.0	38.0	36.0	38.0
68-69	37.13194758726026	38.0	38.0	38.0	36.0	38.0
70-71	37.113136108761935	38.0	38.0	38.0	36.0	38.0
72-73	37.10789274040661	38.0	38.0	38.0	36.0	38.0
74-75	37.06675955335476	38.0	38.0	38.0	36.0	38.0
76-77	37.073039083282296	38.0	38.0	38.0	36.0	38.0
78-79	37.0738073832145	38.0	38.0	38.0	36.0	38.0
80-81	37.0688720732458	38.0	38.0	38.0	36.0	38.0
82-83	37.01731639894769	38.0	38.0	38.0	36.0	38.0
84-85	37.00030830814086	38.0	38.0	38.0	36.0	38.0
86-87	36.96905580734556	38.0	38.0	38.0	36.0	38.0
88-89	36.97338770902632	38.0	38.0	38.0	36.0	38.0
90-91	36.960586906859405	38.0	38.0	38.0	35.5	38.0
92-93	36.937065216001216	38.0	38.0	38.0	35.0	38.0
94-95	36.92022033996756	38.0	38.0	38.0	35.0	38.0
96-97	36.89856497725226	38.0	38.0	38.0	35.0	38.0
98-99	36.92529094865279	38.0	38.0	38.0	35.0	38.0
100	35.42783698700989	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	14.0
21	54.0
22	220.0
23	807.0
24	2068.0
25	4317.0
26	7915.0
27	13634.0
28	21778.0
29	32000.0
30	44442.0
31	58822.0
32	76013.0
33	102147.0
34	151158.0
35	269897.0
36	753075.0
37	4700186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.412446349729876	20.15217633835544	14.51522725145194	23.920150060462746
2	14.261810889510684	20.453239353307563	43.17649067319846	22.10845908398329
3	18.079323990368827	26.097368738690175	30.366333781678932	25.45697348926207
4	12.374137137302727	17.878200911239432	39.32975621203436	30.417905739423478
5	13.297140105588603	37.403903982847304	35.49400566028783	13.804950251276257
6	29.680252725311902	37.73785968282383	18.919021593472333	13.662865998391934
7	26.581182182008728	33.05072180231053	22.971409226059997	17.396686789620745
8	24.27879097585465	36.788408592250256	21.100761843453416	17.832038588441677
9	25.65269953668736	17.296321325846126	21.59579658869558	35.45518254877093
10-11	23.954877670331193	27.038165266555442	28.807438008050585	20.19951905506278
12-13	24.905314566585325	24.745830442705294	29.116075332865705	21.23277965784368
14-15	22.12125506046916	26.02723356317299	27.006940071991064	24.844571304366784
16-17	21.237019423738072	29.305462229055717	27.72003823964279	21.73748010756342
18-19	21.2781988512719	28.254641461672513	29.75656851141098	20.710591175644605
20-21	22.307981817547805	27.225212059802807	29.695242317128358	20.77156380552103
22-23	22.089277039004855	27.07050389731015	29.566108583944573	21.274110479740425
24-25	21.704845220755878	27.291791871505396	29.588070667415366	21.41529224032336
26-27	21.52115722755066	27.41229243451235	29.63012100214575	21.43642933579124
28-29	21.450288066893826	27.38152646603302	29.713796546792544	21.454388920280614
30-31	21.71075678465669	27.324075544125577	29.580595377286755	21.38457229393098
32-33	21.501886353617174	27.458150782333814	29.5394053254719	21.500557538577112
34-35	21.68246773782487	27.4566569475909	29.52015984574417	21.340715468840056
36-37	21.659155419536795	27.35827902444217	29.421987142397732	21.560578413623308
38-39	21.501426509648557	27.480700523758372	29.565773826580905	21.45209914001217
40-41	21.6731064872286	27.396270932842263	29.443221335262535	21.487401244666597
42-43	21.646082249473103	27.424104671815243	29.225266702220893	21.704546376490764
44-45	21.660219511867304	27.51973528945495	29.118485534578177	21.701559664099577
46-47	21.74778646741045	27.555811738481108	28.913666475850096	21.782735318258347
48-49	21.709547242360603	27.70569055639576	28.779848828599082	21.804913372644556
50-51	21.79205502141148	27.88170162644012	28.49775515028192	21.828488201866474
52-53	21.925947559934627	27.852655307037132	28.31938557151559	21.90201156151265
54-55	21.896458611992447	27.836514500954085	28.321448109066203	21.94557877798727
56-57	21.858993091063873	27.91684004470171	28.28917167611001	21.93499518812441
58-59	21.847382737382794	27.845604421384536	28.338378853025432	21.96863398820724
60-61	21.86989832701579	27.87684177764001	28.374090913263935	21.879168982080273
62-63	21.862569897335593	27.90413327303865	28.353664627180912	21.87963220244484
64-65	21.88247476823351	27.776493011226272	28.348050222196402	21.99298199834381
66-67	21.943090326765567	27.738865109524628	28.281998680469645	22.03604588324016
68-69	21.868123794414796	27.7569787001908	28.396688989583595	21.97820851581081
70-71	21.91644312725769	27.62705286065111	28.426080108829115	22.030423903262086
72-73	21.921031450873784	27.76527088037733	28.284650899165502	22.02904676958338
74-75	21.81982099337941	27.881831427306768	28.2125814903678	22.08576608894602
76-77	21.9682941910382	27.804712258605534	28.189129206120654	22.03786434423561
78-79	21.921884586913514	27.756845923035776	28.13399307199651	22.1872764180542
80-81	21.9336324382661	27.919092493595937	28.053488824936096	22.093786243201865
82-83	22.01301614490214	27.82625272451633	28.115339517114858	22.045391613466666
84-85	21.966199394000576	27.89329990187091	28.061699654303673	22.078801049824836
86-87	21.957081219224552	27.930263778192604	28.08565494153851	22.02700006104433
88-89	22.025687188081402	27.89050875493101	28.003747949756562	22.080056107231023
90-91	22.02712365400142	27.92676709961507	28.00953078332621	22.036578463057303
92-93	22.063929155223093	27.93843848116232	27.975110302127394	22.022522061487198
94-95	21.9419334716632	27.946927399810672	28.014495090786628	22.096644037739495
96-97	22.11891700966411	28.027088620554874	28.01665379960101	21.83734057018001
98-99	23.110051466756605	29.945252493970564	28.305360937822964	18.63933510144987
100	21.94700013925083	30.835181722333843	22.294057968131455	24.923760170283874
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2318.0
1	1825.0
2	1399.5
3	1761.0
4	2532.0
5	3480.5
6	4123.0
7	4226.0
8	4111.5
9	4264.0
10	4700.5
11	5826.5
12	7684.0
13	11714.5
14	18058.0
15	24872.0
16	30440.0
17	32769.0
18	32759.0
19	30001.5
20	26406.0
21	24399.0
22	24135.5
23	27024.0
24	32841.5
25	41423.0
26	52546.5
27	64952.0
28	78457.0
29	93521.5
30	109453.5
31	125439.5
32	142613.5
33	159510.5
34	174997.5
35	189905.5
36	206323.0
37	220997.0
38	231163.0
39	240191.0
40	248128.0
41	253756.0
42	256017.0
43	260119.5
44	265284.5
45	268076.5
46	267402.0
47	264206.0
48	261365.5
49	256159.0
50	248621.5
51	240230.0
52	229432.0
53	214703.5
54	197794.0
55	181440.0
56	164404.0
57	145866.5
58	128132.0
59	110445.5
60	93045.0
61	76380.0
62	61045.5
63	47864.0
64	36772.5
65	27787.0
66	20433.5
67	15392.5
68	11593.5
69	8498.5
70	6330.5
71	4628.5
72	3262.5
73	2219.5
74	1520.5
75	1030.5
76	668.5
77	407.5
78	272.5
79	186.5
80	125.5
81	87.5
82	53.0
83	37.0
84	33.5
85	28.0
86	18.5
87	12.5
88	11.5
89	10.5
90	6.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005562188148788372
3	9.777910002192816E-4
4	2.5646977054931977E-4
5	0.0
6	0.0
7	0.0011380846068126066
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.654965686836557E-4
24-25	6.57596750124783E-4
26-27	1.6844482910469968E-4
28-29	0.002688354114176887
30-31	0.0018640143670514248
32-33	0.004445292700781067
34-35	0.009555258065469752
36-37	0.0013791342184778998
38-39	0.0
40-41	1.2331556082067327E-4
42-43	6.871267628837303E-4
44-45	2.921851979980472E-4
46-47	5.900670805116185E-5
48-49	0.0042804845814903
50-51	0.0032158535042871883
52-53	3.906839756452819E-4
54-55	0.002906330627148507
56-57	0.04110781952165655
58-59	0.06220590815277158
60-61	0.029810217877330725
62-63	0.010777420659071507
64-65	7.513079171362341E-4
66-67	1.572849581547995E-4
68-69	0.0015890384018236927
70-71	0.011059644255350658
72-73	0.016743387316049324
74-75	0.011050116565723965
76-77	0.012942966962055017
78-79	0.0026359475178914936
80-81	0.014308764849320952
82-83	0.04477271290580736
84-85	0.025170852619733397
86-87	0.0040933933861842745
88-89	9.334601594536644E-5
90-91	1.0493682645642084E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	584.0
20-21	1763.0
22-23	1117.0
24-25	1198.0
26-27	2253.0
28-29	5227.0
30-31	13773.0
32-33	17035.0
34-35	23706.0
36-37	36986.0
38-39	42636.0
40-41	41657.0
42-43	47338.0
44-45	57260.0
46-47	56921.0
48-49	53234.0
50-51	65404.0
52-53	46329.0
54-55	50463.0
56-57	51869.0
58-59	52046.0
60-61	50749.0
62-63	48940.0
64-65	52378.0
66-67	54772.0
68-69	55134.0
70-71	54129.0
72-73	50968.0
74-75	51507.0
76-77	53786.0
78-79	55229.0
80-81	54216.0
82-83	51037.0
84-85	50411.0
86-87	52197.0
88-89	55118.0
90-91	56256.0
92-93	55054.0
94-95	60186.0
96-97	183333.0
98-99	402833.0
100-101	4021520.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.27332480284682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.58819960525356	80.84794891862514
2	6.177656619643541	10.906445786125019
3	1.2908006477357374	3.418297944999054
4	0.4586114196511635	1.6193261922064734
5	0.2030027250878244	0.8959862743770274
6	0.09996188444654226	0.529438073619256
7	0.05047968683328587	0.3119206854246443
8	0.03887827934358673	0.2745531984217793
9	0.023162808445408033	0.1840192301922864
>10	0.06804558909866001	0.8882360449129887
>50	7.708000152947964E-4	0.04450377753088057
>100	4.04018763994022E-4	0.06518611400071764
>500	2.5915681380617814E-5	0.014137759564789904
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.205872131866497E-5	0.0	0.0	0.0
3	0.0	3.205872131866497E-5	0.0	1.6029360659332486E-5	0.0
4	0.0	6.411744263732994E-5	0.0	1.6029360659332486E-5	0.0
5	0.0	6.411744263732994E-5	0.0	3.205872131866497E-5	0.0
6	0.0	6.411744263732994E-5	0.0	4.808808197799746E-5	0.0
7	0.0	6.411744263732994E-5	0.0	4.808808197799746E-5	0.0
8	0.0	8.014680329666243E-5	0.0	4.808808197799746E-5	0.0
9	0.0	1.4426424593399237E-4	0.0	6.411744263732994E-5	0.0
10-11	0.0	1.4426424593399237E-4	0.0	7.213212296699618E-5	0.0
12-13	0.0	1.6029360659332485E-4	0.0	1.0419084428566116E-4	0.0
14-15	8.014680329666243E-6	2.64484450878986E-4	0.0	1.122055246153274E-4	0.0
16-17	4.808808197799746E-5	2.724991312086523E-4	0.0	1.3624956560432614E-4	0.0
18-19	6.411744263732994E-5	2.8852849186798474E-4	0.0	1.9235232791198984E-4	0.0
20-21	6.411744263732994E-5	3.2860189351631593E-4	0.0	2.244110492306548E-4	0.0
22-23	6.411744263732994E-5	3.766899754943134E-4	0.0	2.5646977054931977E-4	0.0
24-25	6.411744263732994E-5	4.408074181316434E-4	0.0	2.8852849186798474E-4	0.0
26-27	6.411744263732994E-5	4.648514591206421E-4	0.0	4.0073401648331214E-4	0.0
28-29	6.411744263732994E-5	4.648514591206421E-4	0.0	9.136735575819518E-4	0.0
30-31	6.411744263732994E-5	5.93086344395302E-4	0.0	0.0023723453775812077	0.0
32-33	6.411744263732994E-5	5.93086344395302E-4	0.0	0.005602261550436704	0.0
34-35	6.411744263732994E-5	6.812478280216307E-4	0.0	0.0094733521496655	0.0
36-37	6.411744263732994E-5	7.694093116479594E-4	0.0	0.015404215593618518	0.0
38-39	6.411744263732994E-5	8.174973936259568E-4	0.0	0.02470124477603136	0.0
40-41	8.014680329666243E-5	8.174973936259568E-4	0.0	0.0339982739584442	0.0
42-43	8.014680329666243E-5	8.174973936259568E-4	0.0	0.042181262575033435	0.0
44-45	8.014680329666243E-5	9.056588772522855E-4	0.0	0.05152637983942428	0.0
46-47	8.014680329666243E-5	9.938203608786142E-4	0.0	0.06230612488282537	0.0
48-49	8.014680329666243E-5	9.938203608786142E-4	0.0	0.07327822225413846	0.0
50-51	8.014680329666243E-5	0.0011060258854939415	0.0	0.08382554156797924	0.0
52-53	8.014680329666243E-5	0.0011060258854939415	0.0	0.09402822962764437	0.0
54-55	8.014680329666243E-5	0.0011300699264829403	0.0	0.10324511200676054	0.0
56-57	8.014680329666243E-5	0.0011380846068126066	0.0	0.11037016281983383	0.0
58-59	8.014680329666243E-5	0.0011380846068126066	0.0	0.11708646493609415	0.0
60-61	8.014680329666243E-5	0.0011781580084609376	0.0	0.12373063492938746	0.0
62-63	9.617616395599492E-5	0.0012342607707686013	0.0	0.13047899576696642	0.0
64-65	9.617616395599492E-5	0.0013945543773619263	0.0	0.1365461087765238	0.0
66-67	9.617616395599492E-5	0.0014105837380212589	0.0	0.14294182367959746	0.0
68-69	9.617616395599492E-5	0.0014266130986805912	0.0	0.14906503945146246	0.0
70-71	9.617616395599492E-5	0.0014266130986805912	0.0	0.15414634678047084	0.0
72-73	1.122055246153274E-4	0.0014346277790102575	0.0	0.15832199523222695	0.0
74-75	1.122055246153274E-4	0.0014666865003289224	0.0	0.16188852797892844	0.0
76-77	1.122055246153274E-4	0.0014987452216475875	0.0	0.16531079647969593	0.0
78-79	1.122055246153274E-4	0.0015227892626365862	0.0	0.16764306845562882	0.0
80-81	1.122055246153274E-4	0.0015227892626365862	0.0	0.1683323309639801	0.0
82-83	1.122055246153274E-4	0.0015227892626365862	0.0	0.16896549071002376	0.0
84-85	1.122055246153274E-4	0.0015949213856035825	0.0	0.16928607792321038	0.0
86-87	1.122055246153274E-4	0.0016349947872519137	0.0	0.16943034216914438	0.0
88	1.122055246153274E-4	0.001683082869229911	0.0	0.1694463715298037	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10095	0.0	52.23009	1
GTATCAA	18510	0.0	38.018494	1
TCAACGC	22470	0.0	30.957758	4
ATCAACG	22585	0.0	30.782377	3
CAACGCA	22985	0.0	30.245712	5
TATCAAC	23225	0.0	30.154444	2
AACGCAG	23880	0.0	29.094414	6
GTGGTAT	4610	0.0	28.272148	1
TGGTATC	4655	0.0	26.54436	2
ACGCAGA	27225	0.0	25.4731	7
CGCAGAG	27740	0.0	25.045546	8
GCAGAGT	31315	0.0	22.213305	9
GTACATG	25430	0.0	20.634045	1
GAGTACT	16530	0.0	20.541681	12-13
TACATGG	25630	0.0	20.142818	2
ACATGGG	25360	0.0	19.888943	3
CAGAGTA	31050	0.0	19.616156	10-11
GTACTTT	18065	0.0	18.693832	14-15
AGAGTAC	29160	0.0	18.174147	10-11
AGTACTT	17825	0.0	16.996021	12-13
>>END_MODULE