FastQCFastQC Report
Fri 27 May 2016
SRR2139583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6992212
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108100.050.771791
GTATCAA200400.038.6395571
TCAACGC242950.031.3094124
ATCAACG243400.031.199763
CAACGCA248050.030.648535
TATCAAC252700.030.3226342
AACGCAG255650.029.7041236
GTGGTAT49450.028.7368141
TGGTATC49550.027.5625762
ACGCAGA292200.025.9885647
CGCAGAG294850.025.7838468
GCAGAGT340400.022.383629
CTTATAC51750.022.1156851
GTACATG256050.021.2189831
TACATGG259700.020.527572
GAGTACT192000.020.42932312-13
ACATGGG258000.020.1339763
CAGAGTA336500.019.30537410-11
AGAGTAC314450.018.45385410-11
GTACTTT213200.018.40819514-15
Produced by FastQC (version 0.11.5)