##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6992212
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31097598299365	33.0	32.0	33.0	27.0	34.0
2	32.28164249024486	33.0	33.0	34.0	30.0	34.0
3	32.28758910627996	33.0	33.0	33.0	31.0	34.0
4	32.886995703219526	33.0	33.0	34.0	31.0	34.0
5	33.01020177877902	33.0	33.0	34.0	32.0	34.0
6	36.68457149182548	38.0	37.0	38.0	34.0	38.0
7	37.15247606908944	38.0	38.0	38.0	36.0	38.0
8	37.06974473886089	38.0	38.0	38.0	36.0	38.0
9	37.22191518220557	38.0	38.0	38.0	36.0	38.0
10-11	37.341007752625345	38.0	38.0	38.0	37.0	38.0
12-13	37.33979025521538	38.0	38.0	38.0	37.0	38.0
14-15	37.41389698996541	38.0	38.0	38.0	37.0	38.0
16-17	37.43252156828197	38.0	38.0	38.0	37.0	38.0
18-19	37.437297524731804	38.0	38.0	38.0	37.0	38.0
20-21	37.44784898441475	38.0	38.0	38.0	37.0	38.0
22-23	37.45968048528113	38.0	38.0	38.0	37.0	38.0
24-25	37.47180288162204	38.0	38.0	38.0	37.0	38.0
26-27	37.444829501312476	38.0	38.0	38.0	37.0	38.0
28-29	37.42233373043804	38.0	38.0	38.0	37.0	38.0
30-31	37.427359784761336	38.0	38.0	38.0	37.0	38.0
32-33	37.418448727388835	38.0	38.0	38.0	37.0	38.0
34-35	37.39066624101397	38.0	38.0	38.0	37.0	38.0
36-37	37.3757664165326	38.0	38.0	38.0	37.0	38.0
38-39	37.35780840632323	38.0	38.0	38.0	37.0	38.0
40-41	37.35716331438032	38.0	38.0	38.0	37.0	38.0
42-43	37.32834630326141	38.0	38.0	38.0	37.0	38.0
44-45	37.291568516100575	38.0	38.0	38.0	37.0	38.0
46-47	37.24967027063293	38.0	38.0	38.0	37.0	38.0
48-49	37.19569294476721	38.0	38.0	38.0	37.0	38.0
50-51	37.12414529482144	38.0	38.0	38.0	37.0	38.0
52-53	37.14912568277441	38.0	38.0	38.0	37.0	38.0
54-55	37.13298508863478	38.0	38.0	38.0	36.5	38.0
56-57	37.086498812856036	38.0	38.0	38.0	36.0	38.0
58-59	37.07476874097884	38.0	38.0	38.0	36.0	38.0
60-61	37.09352579169672	38.0	38.0	38.0	36.0	38.0
62-63	37.09490912589557	38.0	38.0	38.0	36.0	38.0
64-65	37.04155642085322	38.0	38.0	38.0	36.0	38.0
66-67	37.082724372757355	38.0	38.0	38.0	36.0	38.0
68-69	37.09376647535238	38.0	38.0	38.0	36.0	38.0
70-71	37.07469219719376	38.0	38.0	38.0	36.0	38.0
72-73	37.067983077434604	38.0	38.0	38.0	36.0	38.0
74-75	37.02735241444712	38.0	38.0	38.0	36.0	38.0
76-77	37.034194951268105	38.0	38.0	38.0	36.0	38.0
78-79	37.035651655252416	38.0	38.0	38.0	36.0	38.0
80-81	37.03052800027838	38.0	38.0	38.0	36.0	38.0
82-83	36.982409338572985	38.0	38.0	38.0	36.0	38.0
84-85	36.96885178839689	38.0	38.0	38.0	36.0	38.0
86-87	36.938917843286276	38.0	38.0	38.0	36.0	38.0
88-89	36.9499030752384	38.0	38.0	38.0	36.0	38.0
90-91	36.935653962160124	38.0	38.0	38.0	35.5	38.0
92-93	36.91708378389721	38.0	38.0	38.0	35.0	38.0
94-95	36.90156460880098	38.0	38.0	38.0	35.0	38.0
96-97	36.885491619241016	38.0	38.0	38.0	35.0	38.0
98-99	36.92042745917297	38.0	38.0	38.0	35.0	38.0
100	35.46925899704448	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	17.0
21	64.0
22	330.0
23	1059.0
24	2603.0
25	5476.0
26	9997.0
27	17084.0
28	26524.0
29	39439.0
30	54299.0
31	70664.0
32	91154.0
33	121388.0
34	176139.0
35	303563.0
36	812042.0
37	5260363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16232459770956	20.305548515977488	14.562430315327967	23.969696570984976
2	14.209831516919822	20.487027660974285	43.192554134843675	22.11058668726222
3	18.068245022367293	26.143424985437196	30.27624347319679	25.51208651899872
4	12.363149275356724	18.03220989562797	39.07943695538413	30.525203873631174
5	13.343402631384746	37.32741512986163	35.50284230512462	13.826339933629015
6	29.66437516482624	37.738057713353086	18.837701145216993	13.759865976603683
7	26.565317671563637	32.977300378439054	22.79647312705132	17.660908822945984
8	24.196162816573636	36.88086116382055	21.116894052983522	17.806081966622294
9	25.510696758050244	17.328879616350303	21.578779361952986	35.58164426364647
10-11	23.99639055566393	27.072684581073915	28.66266068591742	20.26826417734474
12-13	24.912917400101716	24.684148592748617	29.010440472914723	21.392493534234948
14-15	22.073851593744585	26.047558340622395	26.82415807758689	25.054431988046126
16-17	21.32960928530199	29.34867392464645	27.55494970690248	21.76676708314908
18-19	21.325190082909383	28.249894310984853	29.598337407389824	20.826578198715943
20-21	22.284053722754514	27.189961386537792	29.56829551433678	20.95768937637092
22-23	22.04620417675323	27.16567999478816	29.431064706714842	21.35705112174377
24-25	21.72901189900131	27.367266754099656	29.51173029063864	21.391991056260395
26-27	21.585868959269337	27.47863774219893	29.550619034478732	21.384874264053
28-29	21.395663835445987	27.312284201559635	29.71418635826503	21.577865604729347
30-31	21.731604801128192	27.23793316709801	29.610134839693213	21.42032719208058
32-33	21.43815228786614	27.534729359025444	29.5174433358557	21.509675017252725
34-35	21.609837771159476	27.525666389929942	29.602587878182973	21.261907960727612
36-37	21.674625795262724	27.388879931437938	29.40875622615079	21.527738047148553
38-39	21.472268815250473	27.408131411980296	29.623377131950413	21.496222640818814
40-41	21.62280946437297	27.283755198291654	29.567680391879737	21.52575494545564
42-43	21.635603856539895	27.34273245042908	29.350059773204016	21.671603919827007
44-45	21.59691070352774	27.496200967414786	29.219365915284317	21.68752241377316
46-47	21.798153750098304	27.472842652717215	28.934389189874366	21.79461440731011
48-49	21.735648157097362	27.564111663904043	28.833833102175145	21.866407076823453
50-51	21.715825716941364	27.731677010309834	28.734175489924873	21.818321782823926
52-53	21.7634948653017	27.73085462230277	28.78717619563243	21.718474316763107
54-55	21.770243140339925	27.72495297661634	28.603998880456395	21.90080500258734
56-57	21.831432756340693	27.86597928169769	28.4407481584893	21.861839803472318
58-59	21.64403401495295	27.896901739514085	28.685449232496925	21.773615013036043
60-61	21.802926886904444	28.030672743133444	28.495372535617346	21.671027834344763
62-63	21.68563156018854	28.13305193687001	28.550204840072563	21.631111662868882
64-65	21.70002150604698	27.976894976003226	28.52066996102898	21.80241355692082
66-67	21.77209257064617	27.977039806917865	28.418993588154574	21.831874034281395
68-69	21.657900106708528	27.937583257870376	28.561162249377535	21.84335438604357
70-71	21.749618052992535	27.68533031896928	28.66287138504174	21.902180242996444
72-73	21.79061428388566	27.761986889707813	28.539450459129707	21.90794836727682
74-75	21.694462730334205	27.870285867394962	28.4864315675511	21.948819834719735
76-77	21.851360299147874	27.725941570921186	28.45923634683753	21.963461783093415
78-79	21.77243401667985	27.68276151156609	28.465956690179834	22.078847781574225
80-81	21.801199684880245	27.84386110749798	28.371847969795063	21.98309123782671
82-83	21.857928193391604	27.75871712288055	28.463949048942478	21.91940563478537
84-85	21.888750139341006	27.783675478505522	28.360032829904487	21.96754155224899
86-87	21.824826916343838	27.855651054313817	28.384020962040186	21.935501067302155
88-89	21.888061369536413	27.77184083773917	28.35688610376197	21.983211688962452
90-91	21.898358204586746	27.817683585360538	28.332205207022337	21.951753003030383
92-93	21.96814719634414	27.82915008833581	28.285824086908768	21.916878628411283
94-95	21.810382914036204	27.8396503203401	28.368236511650352	21.981730253973346
96-97	21.9863916054546	27.96095589728595	28.323254195819324	21.729398301440128
98-99	22.958311162897616	29.917942564245447	28.59964990290203	18.524096369954908
100	21.892717240861227	30.668588971149273	22.669660767998785	24.76903301999071
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2091.0
1	1795.0
2	1825.0
3	2348.5
4	2914.0
5	3944.5
6	5118.5
7	7091.0
8	9556.5
9	10765.5
10	10350.0
11	9943.0
12	10957.5
13	13472.5
14	18567.5
15	24648.0
16	29160.5
17	31416.0
18	30912.0
19	28552.0
20	25856.0
21	23872.5
22	24192.5
23	27823.0
24	34364.5
25	43717.5
26	55692.0
27	69417.0
28	84918.0
29	102922.0
30	121867.0
31	139783.5
32	158941.0
33	177769.5
34	195649.0
35	213019.5
36	230910.0
37	246749.0
38	257847.5
39	267236.5
40	274841.5
41	279343.0
42	280720.5
43	288376.5
44	298927.0
45	302262.5
46	299486.5
47	293678.0
48	287818.5
49	280606.0
50	272609.5
51	262790.5
52	249140.0
53	233553.5
54	217618.0
55	201257.0
56	181881.0
57	161544.0
58	143558.5
59	125265.0
60	105867.5
61	87115.0
62	69726.5
63	54768.0
64	42111.0
65	31355.5
66	22816.5
67	17087.0
68	13061.0
69	9663.0
70	7198.5
71	5235.0
72	3655.5
73	2484.0
74	1627.5
75	1076.5
76	679.5
77	411.5
78	279.5
79	179.0
80	111.5
81	73.0
82	53.0
83	40.0
84	29.5
85	22.5
86	17.0
87	13.0
88	8.0
89	4.5
90	2.5
91	0.5
92	0.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005892269856806401
3	0.0011870349468809012
4	1.8592113625845441E-4
5	0.0
6	0.0
7	9.868121847564119E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.222724952483766E-4
24-25	7.151736534603177E-4
26-27	1.0013604196558467E-4
28-29	0.002962193608831172
30-31	0.0024207746006026294
32-33	0.004836844064022592
34-35	0.009828860542212041
36-37	0.001271363971278732
38-39	0.0
40-41	1.0967426450975897E-4
42-43	7.432500750976932E-4
44-45	2.298843244326325E-4
46-47	7.482748709918002E-5
48-49	0.0042887267495135915
50-51	0.0035176819481013994
52-53	2.532255564439765E-4
54-55	0.00290782091689935
56-57	0.0427715996578272
58-59	0.06566455185792287
60-61	0.03189460966808332
62-63	0.0110437249018538
64-65	8.805929670675196E-4
66-67	1.712932986635697E-4
68-69	0.0014010036790356613
70-71	0.010896111904068898
72-73	0.016688246876931843
74-75	0.011024155991212294
76-77	0.013302770718250062
78-79	0.0029221876068722917
80-81	0.014223592953195615
82-83	0.046332460732752476
84-85	0.025401472963441377
86-87	0.003984435943912875
88-89	4.565314076716816E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	46.0
20-21	232.0
22-23	508.0
24-25	655.0
26-27	1769.0
28-29	4667.0
30-31	13170.0
32-33	17008.0
34-35	23703.0
36-37	37456.0
38-39	44036.0
40-41	42900.0
42-43	49257.0
44-45	59131.0
46-47	61144.0
48-49	56689.0
50-51	51774.0
52-53	49641.0
54-55	53567.0
56-57	55719.0
58-59	55869.0
60-61	56508.0
62-63	53740.0
64-65	57949.0
66-67	62082.0
68-69	63367.0
70-71	67131.0
72-73	56069.0
74-75	56069.0
76-77	58900.0
78-79	60875.0
80-81	60241.0
82-83	56332.0
84-85	55782.0
86-87	57418.0
88-89	60076.0
90-91	60529.0
92-93	60170.0
94-95	67880.0
96-97	206843.0
98-99	456084.0
100-101	4579226.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.98540697247691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.59986017849229	78.80865709275658
2	6.854619679170089	11.925037648683187
3	1.4935204678685232	3.897434571578028
4	0.5124270828442518	1.7829471337970553
5	0.21598551276759623	0.9393793864124231
6	0.11303963844440293	0.5899679372464821
7	0.06470066716329019	0.39396097052126994
8	0.03737261195563327	0.2600697488468173
9	0.026110358429371025	0.20440981387584733
>10	0.08070757732021683	1.0388962653928384
>50	0.0011693894591303486	0.07175343123075413
>100	4.379266163276102E-4	0.05925799193877032
>500	4.890946890170047E-5	0.028228007719998213
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4301625866034955E-5
2	0.0	1.4301625866034955E-5	0.0	0.0	1.4301625866034955E-5
3	0.0	1.4301625866034955E-5	0.0	2.860325173206991E-5	1.4301625866034955E-5
4	0.0	4.2904877598104865E-5	0.0	5.720650346413982E-5	1.4301625866034955E-5
5	0.0	5.720650346413982E-5	0.0	5.720650346413982E-5	1.4301625866034955E-5
6	0.0	5.720650346413982E-5	0.0	5.720650346413982E-5	1.4301625866034955E-5
7	0.0	5.720650346413982E-5	0.0	7.150812933017478E-5	1.4301625866034955E-5
8	0.0	5.720650346413982E-5	0.0	7.150812933017478E-5	1.4301625866034955E-5
9	0.0	7.150812933017478E-5	0.0	2.2882601385655927E-4	1.4301625866034955E-5
10-11	0.0	9.296056812922721E-5	0.0	2.7173089145466413E-4	1.4301625866034955E-5
12-13	1.4301625866034955E-5	1.1441300692827964E-4	0.0	3.14635769052769E-4	1.4301625866034955E-5
14-15	2.1452438799052433E-5	2.1452438799052432E-4	0.0	3.5038983371785636E-4	1.4301625866034955E-5
16-17	2.860325173206991E-5	2.1452438799052432E-4	0.0	3.5754064665087385E-4	1.4301625866034955E-5
18-19	2.860325173206991E-5	2.4312763972259423E-4	0.0	3.7184227251690883E-4	2.860325173206991E-5
20-21	2.860325173206991E-5	2.860325173206991E-4	0.0	4.004455242489787E-4	2.860325173206991E-5
22-23	5.720650346413982E-5	3.14635769052769E-4	0.0	4.7195365357915347E-4	3.575406466508739E-5
24-25	1.0011138106224468E-4	3.3608820785182143E-4	0.0	5.220093441102758E-4	4.2904877598104865E-5
26-27	1.1441300692827964E-4	3.5754064665087385E-4	0.0	5.863666605074331E-4	4.2904877598104865E-5
28-29	1.2156381986129711E-4	3.5754064665087385E-4	0.0	9.15304055426237E-4	4.2904877598104865E-5
30-31	1.4301625866034956E-4	3.7184227251690883E-4	0.0	0.001987925995378859	4.2904877598104865E-5
32-33	1.4301625866034956E-4	3.7184227251690883E-4	0.0	0.0041117174364850495	4.2904877598104865E-5
34-35	1.573178845263845E-4	4.576520277131185E-4	0.0	0.006686010092371341	7.150812933017478E-5
36-37	1.7161951039241946E-4	5.291601570432933E-4	0.0	0.010747671838325267	7.150812933017478E-5
38-39	1.7161951039241946E-4	5.291601570432933E-4	0.0	0.016904521773653314	7.150812933017478E-5
40-41	2.1452438799052432E-4	5.434617829093283E-4	0.0	0.023197237154708693	7.150812933017478E-5
42-43	2.1452438799052432E-4	5.792158475744157E-4	0.0	0.02907520538564906	7.150812933017478E-5
44-45	2.1452438799052432E-4	6.721764157036428E-4	0.0	0.035746913852154366	7.150812933017478E-5
46-47	2.1452438799052432E-4	7.007796674357127E-4	0.0	0.04335537881288497	7.865894226319225E-5
48-49	2.2882601385655927E-4	7.365337321008002E-4	0.0	0.051199820600405135	8.580975519620973E-5
50-51	2.574292655886292E-4	8.723991778281323E-4	0.0	0.058550856295547106	8.580975519620973E-5
52-53	2.8603251732069906E-4	9.081532424932196E-4	0.0	0.06633094076667012	8.580975519620973E-5
54-55	3.14635769052769E-4	9.153040554262371E-4	0.0	0.0734817536996876	8.580975519620973E-5
56-57	3.2893739491880394E-4	9.367564942252896E-4	0.0	0.08121178248027949	8.580975519620973E-5
58-59	3.5038983371785636E-4	9.582089330243419E-4	0.0	0.0888703031315412	8.580975519620973E-5
60-61	3.5754064665087385E-4	9.725105588903769E-4	0.0	0.09583519492830023	8.580975519620973E-5
62-63	3.5754064665087385E-4	9.725105588903769E-4	0.0	0.10169171072044153	8.580975519620973E-5
64-65	3.5754064665087385E-4	0.0010869235658186565	0.0	0.10794152122389883	8.580975519620973E-5
66-67	3.5754064665087385E-4	0.0010940743787516741	0.0	0.11386239433243728	8.580975519620973E-5
68-69	3.5754064665087385E-4	0.0011012251916846915	0.0	0.1201265064617606	8.580975519620973E-5
70-71	3.5754064665087385E-4	0.0011083760046177089	0.0	0.12549676697445672	8.580975519620973E-5
72-73	3.6469145958389134E-4	0.0011155268175507265	0.0	0.12967999254027196	8.580975519620973E-5
74-75	3.7184227251690883E-4	0.0011298284434167615	0.0	0.13279059616613453	8.580975519620973E-5
76-77	3.7184227251690883E-4	0.0012013365727469361	0.0	0.13613717661878671	8.580975519620973E-5
78-79	3.7184227251690883E-4	0.001229939824479006	0.0	0.13863281033240982	8.580975519620973E-5
80-81	3.7184227251690883E-4	0.001229939824479006	0.0	0.13956956682663513	9.296056812922721E-5
82-83	3.7184227251690883E-4	0.0012370906374120235	0.0	0.14019883836474067	1.0011138106224468E-4
84-85	3.7184227251690883E-4	0.0012513922632780587	0.0	0.14044911681739625	1.0011138106224468E-4
86-87	3.7184227251690883E-4	0.0012871463279431458	0.0	0.1405849822631236	1.0011138106224468E-4
88	3.7184227251690883E-4	0.0013729560831393557	0.0	0.14061358551485567	1.0011138106224468E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10810	0.0	50.77179	1
GTATCAA	20040	0.0	38.639557	1
TCAACGC	24295	0.0	31.309412	4
ATCAACG	24340	0.0	31.19976	3
CAACGCA	24805	0.0	30.64853	5
TATCAAC	25270	0.0	30.322634	2
AACGCAG	25565	0.0	29.704123	6
GTGGTAT	4945	0.0	28.736814	1
TGGTATC	4955	0.0	27.562576	2
ACGCAGA	29220	0.0	25.988564	7
CGCAGAG	29485	0.0	25.783846	8
GCAGAGT	34040	0.0	22.38362	9
CTTATAC	5175	0.0	22.115685	1
GTACATG	25605	0.0	21.218983	1
TACATGG	25970	0.0	20.52757	2
GAGTACT	19200	0.0	20.429323	12-13
ACATGGG	25800	0.0	20.133976	3
CAGAGTA	33650	0.0	19.305374	10-11
AGAGTAC	31445	0.0	18.453854	10-11
GTACTTT	21320	0.0	18.408195	14-15
>>END_MODULE