FastQCFastQC Report
Fri 27 May 2016
SRR2139582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8218256
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106100.049.470541
GTATCAA192250.038.4226761
TCAACGC231900.031.0784514
ATCAACG234700.030.6896883
CAACGCA238250.030.321775
TATCAAC242700.029.961032
AACGCAG245850.029.1761516
ACGCAGA279300.025.6819067
GTGGTAT53050.025.5798871
CGCAGAG290650.024.6202958
TGGTATC51600.023.6522922
GCAGAGT334900.021.481919
GAGTACT184000.019.77001812-13
CAGAGTA323550.018.97797610-11
GTACATG252300.018.6220261
AGAGTAC302250.018.31772610-11
GTACTTT200650.018.05533814-15
TACATGG255750.017.8368842
ACATGGG259450.017.2197233
AGTACTT201750.016.40207312-13
Produced by FastQC (version 0.11.5)