##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139582_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8218256
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.79878845341396	33.0	31.0	33.0	25.0	34.0
2	32.08621233020728	33.0	31.0	33.0	29.0	34.0
3	32.153657783354525	33.0	31.0	33.0	30.0	34.0
4	32.82676835571927	33.0	33.0	34.0	31.0	34.0
5	32.944743385944655	33.0	33.0	34.0	31.0	34.0
6	36.59025941756986	38.0	37.0	38.0	34.0	38.0
7	37.09054694816029	38.0	38.0	38.0	36.0	38.0
8	36.99341344440962	38.0	38.0	38.0	36.0	38.0
9	37.16883241407909	38.0	38.0	38.0	36.0	38.0
10-11	37.30321500084689	38.0	38.0	38.0	36.5	38.0
12-13	37.3127298541199	38.0	38.0	38.0	37.0	38.0
14-15	37.39092375559973	38.0	38.0	38.0	37.0	38.0
16-17	37.40364908321182	38.0	38.0	38.0	37.0	38.0
18-19	37.41565594938878	38.0	38.0	38.0	37.0	38.0
20-21	37.43047258638762	38.0	38.0	38.0	37.0	38.0
22-23	37.44369054697551	38.0	38.0	38.0	37.0	38.0
24-25	37.45938221107048	38.0	38.0	38.0	37.0	38.0
26-27	37.435157829177136	38.0	38.0	38.0	37.0	38.0
28-29	37.412418003076375	38.0	38.0	38.0	37.0	38.0
30-31	37.41835383542342	38.0	38.0	38.0	37.0	38.0
32-33	37.40730658811523	38.0	38.0	38.0	37.0	38.0
34-35	37.383701777186715	38.0	38.0	38.0	37.0	38.0
36-37	37.368606074653954	38.0	38.0	38.0	37.0	38.0
38-39	37.35466342268053	38.0	38.0	38.0	37.0	38.0
40-41	37.3478576306225	38.0	38.0	38.0	37.0	38.0
42-43	37.32194082087635	38.0	38.0	38.0	37.0	38.0
44-45	37.288924399172075	38.0	38.0	38.0	37.0	38.0
46-47	37.25441684535568	38.0	38.0	38.0	37.0	38.0
48-49	37.202470404713935	38.0	38.0	38.0	37.0	38.0
50-51	37.128995392059956	38.0	38.0	38.0	36.0	38.0
52-53	37.15026412349593	38.0	38.0	38.0	36.0	38.0
54-55	37.12872553889467	38.0	38.0	38.0	36.0	38.0
56-57	37.07783613227443	38.0	38.0	38.0	36.0	38.0
58-59	37.06204220238506	38.0	38.0	38.0	36.0	38.0
60-61	37.07885199413962	38.0	38.0	38.0	36.0	38.0
62-63	37.076852095576086	38.0	38.0	38.0	36.0	38.0
64-65	37.01680357559457	38.0	38.0	38.0	36.0	38.0
66-67	37.05357238826174	38.0	38.0	38.0	36.0	38.0
68-69	37.06229964719651	38.0	38.0	38.0	36.0	38.0
70-71	37.04108376486419	38.0	38.0	38.0	36.0	38.0
72-73	37.028960836659614	38.0	38.0	38.0	36.0	38.0
74-75	36.98395678848016	38.0	38.0	38.0	36.0	38.0
76-77	36.985514176574114	38.0	38.0	38.0	36.0	38.0
78-79	36.979754569271606	38.0	38.0	38.0	36.0	38.0
80-81	36.9735938204973	38.0	38.0	38.0	36.0	38.0
82-83	36.91858897066321	38.0	38.0	38.0	35.0	38.0
84-85	36.89981741638927	38.0	38.0	38.0	35.0	38.0
86-87	36.86657441715683	38.0	38.0	38.0	35.0	38.0
88-89	36.87082738448824	38.0	38.0	38.0	35.0	38.0
90-91	36.85385744729872	38.0	38.0	38.0	35.0	38.0
92-93	36.828508625069944	38.0	38.0	38.0	35.0	38.0
94-95	36.81230512394637	38.0	38.0	38.0	35.0	38.0
96-97	36.793436754455676	38.0	38.0	38.0	35.0	38.0
98-99	36.82800404539613	38.0	38.0	38.0	35.0	38.0
100	35.32701935913011	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	2.0
20	25.0
21	76.0
22	380.0
23	1257.0
24	3103.0
25	6518.0
26	11741.0
27	19404.0
28	30622.0
29	45589.0
30	62651.0
31	82894.0
32	109077.0
33	146470.0
34	217209.0
35	383135.0
36	1045826.0
37	6052274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.557259837123596	19.378624856660586	14.046836701120045	25.01727860509578
2	14.801633909462296	19.856516971134493	42.305261385114036	23.036587734289174
3	18.717749066611276	24.699431369965076	29.4918104060355	27.091009157388147
4	13.027370935612392	17.204699349970547	37.97555024839251	31.79237946602455
5	14.295003708816079	36.33470410267093	34.900433863340346	14.469858325172641
6	31.333363185571244	36.38720915970493	18.242824268311914	14.036603386411912
7	27.741974421871085	31.883943343749653	22.09735157108138	18.27673066329788
8	24.739166071244313	36.313105846301205	20.557585453653427	18.39014262880105
9	25.681531458742583	17.119459408419498	20.986167868219244	36.212841264618675
10-11	24.72317727751484	26.31634984356778	28.11527774262569	20.84519513629169
12-13	25.527319908262776	23.77408296845462	28.354519499027532	22.344077624255075
14-15	22.651442106451782	25.1184375371125	26.09797626163021	26.132144094805515
16-17	22.33318115181615	28.293277795191585	26.80898477730555	22.564556275686716
18-19	22.337658987502945	27.419911231774723	28.542594681888712	21.699835098833624
20-21	23.067789898060198	26.518865078199926	28.504723304297343	21.908621719442532
22-23	22.80473457316095	26.421699587494828	28.3704999297848	22.403065909559416
24-25	22.576356049967984	26.524320396729628	28.46867185961171	22.43065169369068
26-27	22.489328280494853	26.699509061954878	28.43850762429121	22.372655033259058
28-29	22.4194969797061	26.6728624535316	28.519033233216952	22.38860733354535
30-31	22.66463429905529	26.604564649393126	28.38432442027524	22.346476631276342
32-33	22.478571250622213	26.70315631666335	28.372081833471498	22.44619059924294
34-35	22.640065141892958	26.699475586699208	28.3633332061828	22.29712606522504
36-37	22.58261152582879	26.641493684370015	28.330134463187584	22.445760326613605
38-39	22.436583135961815	26.699300900783264	28.46410429006444	22.400011673190484
40-41	22.691124298057282	26.589578866726832	28.32984425719888	22.389452578017004
42-43	22.582600854557356	26.655760452903248	28.17114718835801	22.590491504181387
44-45	22.583921561302255	26.74563233791463	28.111829869427023	22.558616231356094
46-47	22.688873067961975	26.74334354873974	27.84712697584199	22.7206564074563
48-49	22.69299574377504	26.779724508945662	27.798647922910448	22.728631824368847
50-51	22.59035627688771	27.016717985892065	27.713974748855914	22.67895098836431
52-53	22.762061202271212	26.91568714582412	27.633445327349413	22.68880632455525
54-55	22.742638591549223	26.85616652155059	27.612865112000595	22.78832977489959
56-57	22.739355087731862	26.9960134842519	27.57778215492034	22.686849273095895
58-59	22.67970162158864	26.970567184717076	27.661773841966593	22.68795735172769
60-61	22.768831532549886	26.994126357973652	27.607925254906945	22.62911685456952
62-63	22.70644900115292	27.097729831800255	27.60760201761901	22.588219149427815
64-65	22.697237494738545	26.97684534236609	27.61793563604841	22.70798152684695
66-67	22.733312570794745	26.976355471632225	27.49364568086853	22.7966862767045
68-69	22.647497426243778	26.94521988069727	27.663846417391397	22.743436275667552
70-71	22.69215717485451	26.757170016469793	27.729503541467626	22.82116926720807
72-73	22.75909519889202	26.78376374661473	27.58957762119164	22.867563433301612
74-75	22.642678403148018	26.950979746924936	27.54790817471715	22.858433675209895
76-77	22.79120219406307	26.857278204624365	27.483617738306908	22.86790186300566
78-79	22.713864004375413	26.864744619464105	27.450517918204387	22.970873457956092
80-81	22.740759152446707	26.9418041318334	27.424694684187052	22.89274203153284
82-83	22.86241979106873	26.929918638514323	27.442602640864795	22.765058929552154
84-85	22.886750901315267	26.90951168117408	27.36349019745395	22.840247220056703
86-87	22.768846673183	26.941588768678233	27.441705581393787	22.84785897674498
88-89	22.81909679666483	26.957147352710255	27.374205458462765	22.849550392162158
90-91	22.900644898053116	26.93459308789453	27.35681518572112	22.80794682833123
92-93	22.9006387702394	26.979153234371054	27.351717475417576	22.768490519971973
94-95	22.774137037235658	27.01102947748521	27.357307004006202	22.857526481272927
96-97	22.925168009329035	27.119713834365296	27.352164904359267	22.602953251946406
98-99	24.012713384849228	29.010430353577377	27.597561390841857	19.37929487073154
100	22.910364132719284	29.841921223907207	21.42720810025544	25.82050654311806
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1796.0
1	1600.5
2	1729.0
3	2245.0
4	2759.5
5	3740.0
6	4826.0
7	6372.0
8	8324.5
9	9536.5
10	9505.0
11	9309.5
12	10294.0
13	12897.5
14	17992.5
15	23940.0
16	28373.0
17	30704.0
18	30260.0
19	27626.5
20	24543.0
21	22059.5
22	21475.0
23	24069.0
24	29778.5
25	38497.0
26	50603.0
27	64199.5
28	77988.0
29	94395.5
30	111487.5
31	128174.0
32	147582.0
33	167222.5
34	186050.5
35	204581.5
36	224894.0
37	242781.5
38	256295.0
39	270234.5
40	283801.0
41	295778.5
42	304549.0
43	315927.5
44	328930.0
45	338334.0
46	344329.5
47	348362.0
48	352318.0
49	354766.5
50	354686.0
51	350733.0
52	343448.0
53	330824.0
54	314294.5
55	296089.0
56	274242.0
57	249734.0
58	223492.0
59	195385.0
60	165826.5
61	135889.5
62	108808.0
63	85309.0
64	64356.5
65	47860.0
66	35589.5
67	27063.0
68	20761.5
69	15507.5
70	11452.0
71	8213.5
72	5670.5
73	3757.5
74	2395.5
75	1477.0
76	954.0
77	592.5
78	377.0
79	254.5
80	176.5
81	120.5
82	86.0
83	61.5
84	43.5
85	32.0
86	23.5
87	19.0
88	14.0
89	9.0
90	5.0
91	3.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005110573338187567
3	0.0010099466358799239
4	1.581844128486628E-4
5	0.0
6	0.0
7	7.665860007281351E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	6.084105457016708E-6
22-23	7.666418785792593E-4
24-25	6.145771368542802E-4
26-27	1.0954079646139411E-4
28-29	0.002982947402845099
30-31	0.0022972684929199647
32-33	0.004841903308839489
34-35	0.010370979232879359
36-37	0.0014997840372452927
38-39	0.0
40-41	1.3066906981604843E-4
42-43	8.266326511505632E-4
44-45	1.956268562858853E-4
46-47	3.820111460665458E-5
48-49	0.00441256436274285
50-51	0.003490306467039268
52-53	4.501749027361239E-4
54-55	0.0028400044072660986
56-57	0.04194990698496446
58-59	0.0636102543420651
60-61	0.030122023528355062
62-63	0.011130836558182148
64-65	9.118974089360735E-4
66-67	2.0753271822480387E-4
68-69	0.001480472773616858
70-71	0.011198177489561825
72-73	0.016660542374701152
74-75	0.01036673157069202
76-77	0.012501295269064638
78-79	0.002548208181285974
80-81	0.013920270724857048
82-83	0.044960972322297336
84-85	0.024705340176923718
86-87	0.003929139043397797
88-89	9.999907693159756E-5
90-91	7.774726370449073E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	60.0
20-21	371.0
22-23	682.0
24-25	723.0
26-27	2057.0
28-29	5212.0
30-31	15641.0
32-33	20223.0
34-35	28345.0
36-37	44969.0
38-39	51894.0
40-41	50391.0
42-43	57770.0
44-45	69034.0
46-47	69836.0
48-49	64866.0
50-51	58543.0
52-53	56659.0
54-55	62714.0
56-57	63978.0
58-59	63673.0
60-61	62305.0
62-63	60263.0
64-65	63625.0
66-67	67438.0
68-69	68606.0
70-71	69874.0
72-73	63056.0
74-75	63705.0
76-77	66371.0
78-79	68315.0
80-81	67352.0
82-83	63890.0
84-85	63335.0
86-87	65451.0
88-89	68602.0
90-91	68749.0
92-93	67925.0
94-95	80092.0
96-97	255793.0
98-99	563693.0
100-101	5412175.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.10109415910217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.01087554754285	79.27146840568861
2	6.479703182381513	11.287784740232922
3	1.4530343881687173	3.796826551808905
4	0.49282170266411507	1.7170123810958455
5	0.21366761218861385	0.9305341403995492
6	0.12014807872832267	0.6279017471010512
7	0.06360843038071307	0.3878254718732229
8	0.04156057799836439	0.28959774540337974
9	0.026495839883326707	0.20770349800519208
>10	0.09585304791442821	1.2951064447934035
>50	0.0016954893209685943	0.10183312992305836
>100	4.932213171902472E-4	0.06319255811737855
>500	4.288151086465225E-5	0.02321318555746715
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.433606351517889E-5
2	1.2168031757589445E-5	2.433606351517889E-5	0.0	0.0	2.433606351517889E-5
3	1.2168031757589445E-5	2.433606351517889E-5	0.0	0.0	2.433606351517889E-5
4	1.2168031757589445E-5	4.867212703035778E-5	0.0	1.2168031757589445E-5	2.433606351517889E-5
5	1.2168031757589445E-5	6.084015878794722E-5	0.0	2.433606351517889E-5	2.433606351517889E-5
6	1.2168031757589445E-5	6.084015878794722E-5	0.0	2.433606351517889E-5	2.433606351517889E-5
7	1.2168031757589445E-5	7.300819054553667E-5	0.0	2.433606351517889E-5	2.433606351517889E-5
8	1.2168031757589445E-5	9.734425406071556E-5	0.0	3.6504095272768334E-5	3.6504095272768334E-5
9	1.2168031757589445E-5	1.2168031757589444E-4	0.0	3.6504095272768334E-5	7.300819054553667E-5
10-11	1.2168031757589445E-5	1.2168031757589444E-4	0.0	1.0951228581830501E-4	7.909220642433138E-5
12-13	1.2168031757589445E-5	1.2168031757589444E-4	0.0	1.0951228581830501E-4	9.126023818192085E-5
14-15	1.2168031757589445E-5	2.2510858751540472E-4	0.0	1.1559630169709973E-4	1.0951228581830501E-4
16-17	1.2168031757589445E-5	2.5552866690937835E-4	0.0	1.338483493334839E-4	1.0951228581830501E-4
18-19	1.2168031757589445E-5	2.737807145457625E-4	0.0	1.338483493334839E-4	1.0951228581830501E-4
20-21	1.2168031757589445E-5	2.9203276218214667E-4	0.0	1.5210039696986807E-4	1.0951228581830501E-4
22-23	1.2168031757589445E-5	3.224528415761203E-4	0.0	2.0077252400022585E-4	1.2168031757589446E-4
24-25	1.2168031757589445E-5	3.4070488921250446E-4	0.0	2.3727661927299418E-4	1.338483493334839E-4
26-27	1.2168031757589445E-5	3.528729209700939E-4	0.0	3.4678890509129916E-4	1.338483493334839E-4
28-29	1.2168031757589445E-5	3.528729209700939E-4	0.0	6.814097784250089E-4	1.338483493334839E-4
30-31	1.2168031757589445E-5	3.9546103212165695E-4	0.0	0.001861708858911185	1.399323652122786E-4
32-33	1.2168031757589445E-5	4.0762906387924636E-4	0.0	0.004325735289823047	1.7035244460625223E-4
34-35	1.2168031757589445E-5	4.8672127030357777E-4	0.0	0.007221726848129335	1.7035244460625223E-4
36-37	1.2168031757589445E-5	5.597294608491144E-4	0.0	0.0116448063920131	1.7035244460625223E-4
38-39	1.2168031757589445E-5	5.779815084854987E-4	0.0	0.018209459525232605	1.7035244460625223E-4
40-41	1.2168031757589445E-5	6.814097784250089E-4	0.0	0.025552866690937835	1.7035244460625223E-4
42-43	1.2168031757589445E-5	7.057458419401877E-4	0.0	0.0323913005387031	1.7035244460625223E-4
44-45	2.433606351517889E-5	7.726700166069297E-4	0.0	0.040118000704772394	1.886044922426364E-4
46-47	2.433606351517889E-5	8.152581277584928E-4	0.0	0.04825232993472094	2.129405557578153E-4
48-49	2.433606351517889E-5	8.213421436372875E-4	0.0	0.0561372145136389	2.1902457163661002E-4
50-51	2.433606351517889E-5	9.673585247283609E-4	0.0	0.06453924044225441	2.1902457163661002E-4
52-53	2.433606351517889E-5	9.977786041223346E-4	0.0	0.07226594060832371	2.3119260339419945E-4
54-55	2.433606351517889E-5	0.0010038626200011293	0.0	0.07979795226627158	2.4336063515178888E-4
56-57	3.042007939397361E-5	0.0010099466358799239	0.0	0.08567511160518729	2.4336063515178888E-4
58-59	3.6504095272768334E-5	0.001034282699395103	0.0	0.09166178322992129	2.4336063515178888E-4
60-61	3.6504095272768334E-5	0.001076870810546666	0.0	0.09747810241004903	2.4336063515178888E-4
62-63	4.258811115156306E-5	0.001113374905819434	0.0	0.10283203638338839	2.4336063515178888E-4
64-65	4.867212703035778E-5	0.0012776433345468917	0.0	0.10855709532533422	2.4336063515178888E-4
66-67	4.867212703035778E-5	0.0013080634139408652	0.0	0.11444033868012873	2.4336063515178888E-4
68-69	4.867212703035778E-5	0.0013263154615772495	0.0	0.12056085865419622	2.4336063515178888E-4
70-71	4.867212703035778E-5	0.0013445675092136336	0.0	0.12573835616705054	2.4336063515178888E-4
72-73	4.867212703035778E-5	0.001356735540971223	0.0	0.12976597467881262	2.676966986669678E-4
74-75	4.867212703035778E-5	0.0014175756997591702	0.0	0.1332460317614832	2.676966986669678E-4
76-77	4.867212703035778E-5	0.0015088359379410912	0.0	0.1363975519866989	2.676966986669678E-4
78-79	4.867212703035778E-5	0.0015453400332138595	0.0	0.1384417813219739	2.676966986669678E-4
80-81	4.867212703035778E-5	0.0015453400332138595	0.0	0.13912319110039892	2.676966986669678E-4
82-83	4.867212703035778E-5	0.0015575080649714488	0.0	0.13964641646597525	2.798647304245572E-4
84-85	4.867212703035778E-5	0.0015696760967290383	0.0	0.13990194513288465	2.798647304245572E-4
86-87	4.867212703035778E-5	0.0016183482237593961	0.0	0.13999320537106658	2.798647304245572E-4
88	4.867212703035778E-5	0.001776532636608059	0.0	0.14000537340282415	2.798647304245572E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10610	0.0	49.47054	1
GTATCAA	19225	0.0	38.422676	1
TCAACGC	23190	0.0	31.078451	4
ATCAACG	23470	0.0	30.689688	3
CAACGCA	23825	0.0	30.32177	5
TATCAAC	24270	0.0	29.96103	2
AACGCAG	24585	0.0	29.176151	6
ACGCAGA	27930	0.0	25.681906	7
GTGGTAT	5305	0.0	25.579887	1
CGCAGAG	29065	0.0	24.620295	8
TGGTATC	5160	0.0	23.652292	2
GCAGAGT	33490	0.0	21.48191	9
GAGTACT	18400	0.0	19.770018	12-13
CAGAGTA	32355	0.0	18.977976	10-11
GTACATG	25230	0.0	18.622026	1
AGAGTAC	30225	0.0	18.317726	10-11
GTACTTT	20065	0.0	18.055338	14-15
TACATGG	25575	0.0	17.836884	2
ACATGGG	25945	0.0	17.219723	3
AGTACTT	20175	0.0	16.402073	12-13
>>END_MODULE