FastQCFastQC Report
Fri 27 May 2016
SRR2139581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5814992
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.050.731171
GTATCAA131900.038.7755621
TCAACGC150500.032.970834
TATCAAC152600.032.746872
ATCAACG153200.032.3900343
CAACGCA154400.032.138025
AACGCAG160450.030.9532896
ACGCAGA186700.026.5779887
CGCAGAG192850.025.7304138
GTGGTAT37300.024.8140261
TGGTATC34800.024.598772
GCAGAGT225350.022.1738229
GAGTACT130500.020.7266512-13
GTACATG175600.020.390561
TACATGG176100.019.5430472
CAGAGTA220150.019.52978310-11
ACATGGG178550.018.933193
GTACTTT143550.018.87316514-15
GATCAAC27050.018.3131921
AGAGTAC203000.017.64797810-11
Produced by FastQC (version 0.11.5)