##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139581_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5814992
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.309157089124113	25.0	18.0	33.0	18.0	33.0
2	27.681566027949824	29.0	25.0	31.0	18.0	33.0
3	27.06593543034969	29.0	25.0	31.0	18.0	33.0
4	31.04376927775653	31.0	30.0	33.0	28.0	33.0
5	31.74955339577423	33.0	31.0	33.0	29.0	33.0
6	36.087244144102	38.0	36.0	38.0	33.0	38.0
7	36.36993017359267	38.0	37.0	38.0	34.0	38.0
8	36.491926558110485	38.0	37.0	38.0	34.0	38.0
9	37.16083702264767	38.0	38.0	38.0	36.0	38.0
10-11	37.21410502370425	38.0	38.0	38.0	36.0	38.0
12-13	37.318023481373665	38.0	38.0	38.0	36.5	38.0
14-15	37.30582613011333	38.0	38.0	38.0	36.0	38.0
16-17	37.41638698729078	38.0	38.0	38.0	37.0	38.0
18-19	37.440693813508254	38.0	38.0	38.0	37.0	38.0
20-21	37.47239398201245	38.0	38.0	38.0	37.0	38.0
22-23	37.49903494839282	38.0	38.0	38.0	37.0	38.0
24-25	37.51032249508312	38.0	38.0	38.0	37.0	38.0
26-27	37.47799034337319	38.0	38.0	38.0	37.0	38.0
28-29	37.45252667326123	38.0	38.0	38.0	37.0	38.0
30-31	37.46555276189948	38.0	38.0	38.0	37.0	38.0
32-33	37.456289171920886	38.0	38.0	38.0	37.0	38.0
34-35	37.42463271426112	38.0	38.0	38.0	37.0	38.0
36-37	37.40681975321928	38.0	38.0	38.0	37.0	38.0
38-39	37.396273524225535	38.0	38.0	38.0	37.0	38.0
40-41	37.39643034258718	38.0	38.0	38.0	37.0	38.0
42-43	37.37259584246483	38.0	38.0	38.0	37.0	38.0
44-45	37.342209921514346	38.0	38.0	38.0	37.0	38.0
46-47	37.30891326034468	38.0	38.0	38.0	37.0	38.0
48-49	37.2551106075788	38.0	38.0	38.0	37.0	38.0
50-51	37.179176731392474	38.0	38.0	38.0	37.0	38.0
52-53	37.20434491252725	38.0	38.0	38.0	37.0	38.0
54-55	37.18753163642498	38.0	38.0	38.0	37.0	38.0
56-57	37.138835651289995	38.0	38.0	38.0	36.0	38.0
58-59	37.12624424363159	38.0	38.0	38.0	36.0	38.0
60-61	37.14519236405846	38.0	38.0	38.0	36.0	38.0
62-63	37.148866143491844	38.0	38.0	38.0	36.0	38.0
64-65	37.09128806372598	38.0	38.0	38.0	36.0	38.0
66-67	37.13502297731015	38.0	38.0	38.0	36.0	38.0
68-69	37.14488144158118	38.0	38.0	38.0	36.0	38.0
70-71	37.1270657991256	38.0	38.0	38.0	36.0	38.0
72-73	37.12076408750224	38.0	38.0	38.0	36.0	38.0
74-75	37.07749634503932	38.0	38.0	38.0	36.0	38.0
76-77	37.08398397522807	38.0	38.0	38.0	36.0	38.0
78-79	37.08284272698421	38.0	38.0	38.0	36.0	38.0
80-81	37.07680685165627	38.0	38.0	38.0	36.0	38.0
82-83	37.02764615144001	38.0	38.0	38.0	36.0	38.0
84-85	37.01679072800489	38.0	38.0	38.0	36.0	38.0
86-87	36.98689646530264	38.0	38.0	38.0	36.0	38.0
88-89	36.99544176689217	38.0	38.0	38.0	36.0	38.0
90-91	36.98289026545483	38.0	38.0	38.0	36.0	38.0
92-93	36.96438716781263	38.0	38.0	38.0	36.0	38.0
94-95	36.94819754259963	38.0	38.0	38.0	35.5	38.0
96-97	36.93045786946074	38.0	38.0	38.0	35.0	38.0
98-99	36.96417238480165	38.0	38.0	38.0	35.0	38.0
100	35.54611927782052	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	8.0
21	56.0
22	274.0
23	890.0
24	2294.0
25	4542.0
26	8302.0
27	13938.0
28	22311.0
29	32452.0
30	43447.0
31	56804.0
32	74407.0
33	102788.0
34	162608.0
35	331206.0
36	1174064.0
37	3784597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.050832744051924	18.649122819085566	10.46231877877046	21.83772565809205
2	15.13644706739797	19.838123938165573	40.961464148755105	24.063964845681355
3	19.900374053022976	24.896323288020827	29.058318806125794	26.1449838528304
4	13.29858290335251	17.22964862524222	38.15244330684776	31.319325164557522
5	14.179709963487483	37.17989637818934	34.52983254319181	14.11056111513137
6	31.28723134958741	36.55826181704119	18.21235523625828	13.942151597113117
7	27.66299264433133	32.25372921034344	22.027298158494684	18.055979986830543
8	24.657230826800795	36.56806406612425	20.582865806178237	18.191839300896717
9	25.67613162666432	17.329241381587455	21.126649873293033	35.867977118455194
10-11	24.65895911808649	26.56758599151985	28.09065773435286	20.6827971560408
12-13	25.355236946155728	24.019671910124725	28.47033151550337	22.15475962821617
14-15	22.523049042887763	25.456827799591125	26.10847959894012	25.911643558580995
16-17	22.172584244311942	28.66755448674736	26.875840929789756	22.28402033915094
18-19	22.15601500397593	27.72242850893002	28.60191381174729	21.51964267534676
20-21	22.847272947708337	26.869409060208348	28.638518159927052	21.644799832156263
22-23	22.596689581850562	26.788107080610384	28.540664197237227	22.074539140301823
24-25	22.39067994053024	26.884054001597935	28.654852229819376	22.07041382805245
26-27	22.3090878881478	27.061100200586246	28.58344521924651	22.046366692019447
28-29	22.22227574931599	27.014912982867557	28.6317743615066	22.131036906309856
30-31	22.53244915758439	26.91923108080525	28.53068899024194	22.017630771368417
32-33	22.3113196891952	27.081275961255102	28.518483456616156	22.08892089293354
34-35	22.433087501906044	27.083875989686394	28.521274936814283	21.961761571593275
36-37	22.420584122135526	26.923685743389697	28.487921408208916	22.167808726265864
38-39	22.23993757513363	27.088262482362957	28.600019003109413	22.071780939394003
40-41	22.506319880817863	26.929519626116914	28.447916996305196	22.11624349676003
42-43	22.375567067643505	26.98755765109015	28.403501718189816	22.233373563076526
44-45	22.392654406340146	27.09401988805789	28.28169300295538	22.231632702646586
46-47	22.518196980141873	27.064457737552537	27.9804487535858	22.436896528719785
48-49	22.45848363326146	27.163004610317348	27.98736249095961	22.39114926546158
50-51	22.377212923711532	27.360380601831785	27.909277710461712	22.353128763994974
52-53	22.534619519091688	27.277558672730116	27.83578026067624	22.35204154750195
54-55	22.551946231097325	27.18571996894694	27.844758775424328	22.417575024531406
56-57	22.530523536662102	27.34561522975708	27.79177256802138	22.33208866555944
58-59	22.446388195384937	27.39135081637632	27.857797464699196	22.304463523539553
60-61	22.488576800287813	27.428836945825218	27.848774772970327	22.23381148091664
62-63	22.41867996611289	27.490315594091268	27.839850161220305	22.251154278575537
64-65	22.404342683501458	27.461822205202147	27.815142750852683	22.318692360443713
66-67	22.48815474781119	27.491930558527343	27.651970205489995	22.36794448817147
68-69	22.359676913874647	27.446492680951252	27.773773411053927	22.420056994120177
70-71	22.375551983273677	27.26436857598174	27.834000623216276	22.526078817528305
72-73	22.525801023938605	27.20166836335088	27.745169568807558	22.52736104390296
74-75	22.35909543669009	27.363970656512244	27.705712609943014	22.571221296854656
76-77	22.570363321962134	27.27150154816397	27.62120680013255	22.536928329741343
78-79	22.529658881028258	27.27244205733492	27.570422200984474	22.627476860652344
80-81	22.47766409992448	27.324753551410634	27.5496804259777	22.647901922687186
82-83	22.59028959673503	27.283490384971564	27.615378916504486	22.51084110178892
84-85	22.649890786627736	27.27723530278643	27.588311767745992	22.48456214283984
86-87	22.547396195386884	27.327579532577523	27.589779620267368	22.535244651768224
88-89	22.573797682432673	27.33917629290548	27.575023452581394	22.512002572080455
90-91	22.629831464033924	27.368950310426015	27.52067476405186	22.480543461488196
92-93	22.68984798272106	27.35222459556536	27.5508710727911	22.40705634892248
94-95	22.573540892122306	27.45180514231279	27.52629913356483	22.44835483200007
96-97	22.73704730052556	27.513119923554708	27.5785680158351	22.171264760084636
98-99	23.75255245539754	29.39547881581397	27.781420329494445	19.070548399294044
100	22.69345080576327	30.19549376303389	21.75141151473233	25.35964391647051
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	707.0
1	748.0
2	1002.5
3	1385.5
4	1757.0
5	2408.0
6	3227.5
7	4106.5
8	5729.0
9	6955.5
10	6708.5
11	6365.0
12	6888.5
13	8434.0
14	11370.5
15	14627.5
16	17442.0
17	19913.5
18	20563.5
19	19186.5
20	17266.5
21	15748.0
22	15666.0
23	17849.5
24	22265.5
25	28885.5
26	37427.0
27	47640.0
28	58436.0
29	70527.0
30	82993.0
31	94796.5
32	107721.0
33	120348.0
34	132696.5
35	145475.5
36	159747.0
37	172279.0
38	182025.5
39	191551.0
40	201143.0
41	210413.5
42	216661.0
43	222534.5
44	229757.0
45	237789.5
46	241915.0
47	242849.0
48	243148.5
49	241493.0
50	240120.0
51	236378.0
52	228950.5
53	218740.5
54	206727.0
55	193421.5
56	178878.5
57	162968.0
58	146361.0
59	128705.0
60	108932.0
61	88787.5
62	70509.5
63	54690.5
64	40670.0
65	29681.0
66	22035.5
67	16707.0
68	12777.0
69	9553.0
70	7004.5
71	5037.5
72	3452.0
73	2215.0
74	1407.5
75	887.5
76	572.5
77	362.5
78	231.5
79	151.5
80	89.5
81	54.5
82	36.0
83	25.0
84	16.0
85	10.5
86	6.5
87	4.5
88	3.5
89	3.0
90	2.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0029750685813497253
3	7.05074056851669E-4
4	6.878771286357746E-5
5	0.0
6	0.0
7	3.611354925337816E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.213438702549801E-4
24-25	3.783705832006385E-4
26-27	2.5799973322827583E-5
28-29	0.0028473691427423143
30-31	0.002109126007452016
32-33	0.004379672045329261
34-35	0.009441868926818
36-37	0.001516196180883764
38-39	0.0
40-41	1.0490143199196454E-4
42-43	6.676119231624355E-4
44-45	1.7670388934095635E-4
46-47	3.558005360846677E-5
48-49	0.00419142901129832
50-51	0.0033889734880423765
52-53	4.7135387608342275E-4
54-55	0.0027985621407049586
56-57	0.04276429189880334
58-59	0.0657460921370277
60-61	0.031280280985233104
62-63	0.011251996129388219
64-65	9.050475065092715E-4
66-67	2.470328034859749E-4
68-69	0.0015904369898086521
70-71	0.011249102681113453
72-73	0.017176768936680988
74-75	0.011072789195315747
76-77	0.01269873092924499
78-79	0.0030573428633174906
80-81	0.013922394095291883
82-83	0.04617013210516781
84-85	0.024623900696733272
86-87	0.004088299400965812
88-89	4.1781666351040994E-5
90-91	2.1094810096608957E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	27.0
20-21	161.0
22-23	341.0
24-25	350.0
26-27	1121.0
28-29	2979.0
30-31	8064.0
32-33	10610.0
34-35	14919.0
36-37	23520.0
38-39	26539.0
40-41	27147.0
42-43	31040.0
44-45	37244.0
46-47	38765.0
48-49	36421.0
50-51	32207.0
52-53	30414.0
54-55	33167.0
56-57	34422.0
58-59	36994.0
60-61	38059.0
62-63	37198.0
64-65	40249.0
66-67	43163.0
68-69	44429.0
70-71	46115.0
72-73	40369.0
74-75	40634.0
76-77	42788.0
78-79	44697.0
80-81	44620.0
82-83	42448.0
84-85	42168.0
86-87	43612.0
88-89	45727.0
90-91	46545.0
92-93	46459.0
94-95	55218.0
96-97	182893.0
98-99	406136.0
100-101	4015013.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.88456528647376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.4316042607764	80.35426793903235
2	6.266090932997001	11.013853553839125
3	1.3428734781838005	3.5405355559475495
4	0.46220047649735374	1.6248115180868388
5	0.19578291291152922	0.8603148095874649
6	0.1026586917508929	0.5413268698443199
7	0.05969096020767261	0.36721398625684576
8	0.03561606968594601	0.25040822412496966
9	0.0262259280013019	0.2074368853465899
>10	0.07487099384644129	1.036289233143801
>50	0.0018195725034401025	0.1098792365283742
>100	5.370196471885571E-4	0.07888004589441845
>500	2.8702991063618742E-5	0.014782142367399375
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.7196928215894364E-5	0.0	0.0	0.0
4	0.0	5.159078464768309E-5	0.0	0.0	0.0
5	0.0	5.159078464768309E-5	0.0	1.7196928215894364E-5	0.0
6	8.598464107947182E-5	5.159078464768309E-5	0.0	3.439385643178873E-5	0.0
7	8.598464107947182E-5	5.159078464768309E-5	0.0	3.439385643178873E-5	0.0
8	8.598464107947182E-5	5.159078464768309E-5	0.0	3.439385643178873E-5	0.0
9	1.0318156929536618E-4	1.0318156929536618E-4	0.0	6.878771286357746E-5	0.0
10-11	1.2037849751126055E-4	1.0318156929536618E-4	0.0	8.598464107947182E-5	0.0
12-13	1.2037849751126055E-4	1.0318156929536618E-4	0.0	9.4583105187419E-5	0.0
14-15	1.3757542572715491E-4	1.0318156929536618E-4	0.0	1.0318156929536618E-4	0.0
16-17	2.0636313859073236E-4	1.1178003340331336E-4	0.0	1.0318156929536618E-4	0.0
18-19	2.0636313859073236E-4	1.805677462668908E-4	0.0	1.2897696161920773E-4	0.0
20-21	2.0636313859073236E-4	2.321585309145739E-4	0.0	1.5477235394304928E-4	0.0
22-23	2.0636313859073236E-4	2.923477796702042E-4	0.0	1.8916621037483802E-4	0.0
24-25	2.2356006680662673E-4	3.439385643178873E-4	0.0	2.0636313859073236E-4	0.0
26-27	2.2356006680662673E-4	3.7833242074967604E-4	0.0	2.5795392323841546E-4	0.0
28-29	2.2356006680662673E-4	3.869308848576232E-4	0.0	5.503017029086197E-4	0.0
30-31	2.5795392323841546E-4	4.987109182609366E-4	0.0	0.0012897696161920773	0.0
32-33	2.6655238734636265E-4	5.24506310584778E-4	0.0	0.0031986286481563517	0.0
34-35	3.0954470788609856E-4	6.1049095166425E-4	0.0	0.005803963272864348	0.0
36-37	3.439385643178873E-4	6.964755927437217E-4	0.0	0.010060203006298203	0.0
38-39	3.439385643178873E-4	7.308694491755105E-4	0.0	0.021685326480242795	0.0
40-41	3.439385643178873E-4	8.770433390106125E-4	0.0	0.03454003032162383	0.0
42-43	3.525370284258345E-4	9.114371954424013E-4	0.0	0.0421152772007253	0.0
44-45	3.6113549253378167E-4	0.0011178003340331336	0.0	0.05113506604996189	0.0
46-47	3.6113549253378167E-4	0.0013155650085159187	0.0	0.06121246598447599	0.0
48-49	3.6113549253378167E-4	0.001324163472623866	0.0	0.0703612317953318	0.0
50-51	4.127262771814647E-4	0.0015391250753225456	0.0	0.08058480561968098	0.0
52-53	4.213247412894119E-4	0.0015649204676463871	0.0	0.11234753203443788	0.0
54-55	4.299232053973591E-4	0.0015821173958622814	0.0	0.1504731218890757	0.0
56-57	4.299232053973591E-4	0.0015993143240781759	0.0	0.20638033551894824	8.598464107947182E-6
58-59	4.299232053973591E-4	0.0015993143240781759	0.0	0.2428722171930761	1.7196928215894364E-5
60-61	4.815139900450422E-4	0.0016251097164020174	0.0	0.25509063469046905	1.7196928215894364E-5
62-63	5.846955593404084E-4	0.0016337081805099646	0.0	0.26650939502582294	2.5795392323841545E-5
64-65	5.846955593404084E-4	0.0016681020369417533	0.0	0.27260570607835743	3.439385643178873E-5
66-67	6.018924875563028E-4	0.0016852989651576476	0.0	0.2779969430740403	3.439385643178873E-5
68-69	6.362863439880915E-4	0.0016938974292655948	0.0	0.28339677853383116	3.439385643178873E-5
70-71	6.706802004198802E-4	0.001711094357481489	0.0	0.28793676758282727	3.439385643178873E-5
72-73	7.222709850675633E-4	0.0017282912856973836	0.0	0.29163410714924454	3.439385643178873E-5
74-75	7.308694491755105E-4	0.0017626851421291723	0.0	0.2943770171996797	3.439385643178873E-5
76-77	7.394679132834577E-4	0.001797078998560961	0.0	0.29724030574762617	3.439385643178873E-5
78-79	7.394679132834577E-4	0.0018400713191006968	0.0	0.2991319678513745	3.439385643178873E-5
80-81	7.394679132834577E-4	0.0018572682473165913	0.0	0.2998026480517944	3.439385643178873E-5
82-83	7.394679132834577E-4	0.0018572682473165913	0.0	0.3002755635777315	3.439385643178873E-5
84-85	7.566648414993521E-4	0.0019002605678563273	0.0	0.3004389343957825	3.439385643178873E-5
86-87	7.566648414993521E-4	0.0019088590319642745	0.0	0.30054211596507785	3.439385643178873E-5
88	7.566648414993521E-4	0.0019948436730437462	0.0	0.3005507144291858	3.439385643178873E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7195	0.0	50.73117	1
GTATCAA	13190	0.0	38.775562	1
TCAACGC	15050	0.0	32.97083	4
TATCAAC	15260	0.0	32.74687	2
ATCAACG	15320	0.0	32.390034	3
CAACGCA	15440	0.0	32.13802	5
AACGCAG	16045	0.0	30.953289	6
ACGCAGA	18670	0.0	26.577988	7
CGCAGAG	19285	0.0	25.730413	8
GTGGTAT	3730	0.0	24.814026	1
TGGTATC	3480	0.0	24.59877	2
GCAGAGT	22535	0.0	22.173822	9
GAGTACT	13050	0.0	20.72665	12-13
GTACATG	17560	0.0	20.39056	1
TACATGG	17610	0.0	19.543047	2
CAGAGTA	22015	0.0	19.529783	10-11
ACATGGG	17855	0.0	18.93319	3
GTACTTT	14355	0.0	18.873165	14-15
GATCAAC	2705	0.0	18.313192	1
AGAGTAC	20300	0.0	17.647978	10-11
>>END_MODULE