FastQCFastQC Report
Fri 27 May 2016
SRR2139580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5217106
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66300.048.9928551
GTATCAA117450.035.5217821
TCAACGC128450.032.0808874
ATCAACG132050.031.109573
CAACGCA133100.030.9917895
TATCAAC133200.030.9060842
AACGCAG140850.029.316756
ACGCAGA164300.025.0806477
CGCAGAG172450.023.8953328
TGGTATC32750.023.1382472
GTGGTAT36550.022.4797611
GCAGAGT200000.020.688899
GTACATG170600.020.287721
GAGTACT105400.019.93184312-13
TACATGG173300.019.5540282
CAGAGTA195850.018.77980610-11
ACATGGG174000.018.5204563
GTACTTT114150.018.21779614-15
CATGGGG122100.016.595734
GATCAAC24250.016.5020471
Produced by FastQC (version 0.11.5)