FastQCFastQC Report
Fri 27 May 2016
SRR2139579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6967452
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101300.045.826461
GTATCAA181600.036.771411
TCAACGC205150.031.992414
ATCAACG208450.031.4047323
TATCAAC209050.031.3964672
CAACGCA212150.030.8769635
AACGCAG220350.029.7471286
ACGCAGA261150.025.0996727
CGCAGAG267800.024.4762238
GCAGAGT307050.021.5266029
GTACATG238000.021.3542921
TACATGG237950.020.7007662
ACATGGG236400.020.2451883
GAGTACT167350.020.19076312-13
GTGGTAT54700.019.9595851
TGGTATC53150.018.7900492
CAGAGTA296750.018.71596110-11
GTACTTT182350.018.40258214-15
CATGGGG167050.017.8840084
GATCAAC34250.017.7918471
Produced by FastQC (version 0.11.5)