##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139579_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6967452
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.353475129789196	25.0	18.0	33.0	18.0	33.0
2	27.595625057768608	29.0	25.0	31.0	18.0	33.0
3	26.947367272856706	29.0	25.0	31.0	18.0	33.0
4	30.9807925479788	31.0	30.0	33.0	28.0	33.0
5	31.712796155610402	33.0	31.0	33.0	29.0	33.0
6	36.07208158735791	38.0	36.0	38.0	33.0	38.0
7	36.37000154432352	38.0	37.0	38.0	34.0	38.0
8	36.51115371874826	38.0	37.0	38.0	34.0	38.0
9	37.17386642921975	38.0	38.0	38.0	36.0	38.0
10-11	37.226009163751684	38.0	38.0	38.0	36.0	38.0
12-13	37.32782895382702	38.0	38.0	38.0	36.5	38.0
14-15	37.31288704967038	38.0	38.0	38.0	36.0	38.0
16-17	37.41786875603879	38.0	38.0	38.0	37.0	38.0
18-19	37.44210344039686	38.0	38.0	38.0	37.0	38.0
20-21	37.47210998823545	38.0	38.0	38.0	37.0	38.0
22-23	37.50038399837222	38.0	38.0	38.0	37.0	38.0
24-25	37.51148149542337	38.0	38.0	38.0	37.0	38.0
26-27	37.48015730301692	38.0	38.0	38.0	37.0	38.0
28-29	37.45686423442301	38.0	38.0	38.0	37.0	38.0
30-31	37.47074647135109	38.0	38.0	38.0	37.0	38.0
32-33	37.460858542492105	38.0	38.0	38.0	37.0	38.0
34-35	37.42897186604145	38.0	38.0	38.0	37.0	38.0
36-37	37.41226538277459	38.0	38.0	38.0	37.0	38.0
38-39	37.40711410082644	38.0	38.0	38.0	37.0	38.0
40-41	37.398483197987986	38.0	38.0	38.0	37.0	38.0
42-43	37.371982116311735	38.0	38.0	38.0	37.0	38.0
44-45	37.33965970218202	38.0	38.0	38.0	37.0	38.0
46-47	37.3036590956028	38.0	38.0	38.0	37.0	38.0
48-49	37.248070926297125	38.0	38.0	38.0	37.0	38.0
50-51	37.16956425180318	38.0	38.0	38.0	37.0	38.0
52-53	37.196508454452584	38.0	38.0	38.0	37.0	38.0
54-55	37.1808718299736	38.0	38.0	38.0	37.0	38.0
56-57	37.13411179014251	38.0	38.0	38.0	36.0	38.0
58-59	37.12225648772154	38.0	38.0	38.0	36.0	38.0
60-61	37.1424764808788	38.0	38.0	38.0	36.0	38.0
62-63	37.146198160317326	38.0	38.0	38.0	36.0	38.0
64-65	37.09244086904553	38.0	38.0	38.0	36.0	38.0
66-67	37.13449179361621	38.0	38.0	38.0	36.0	38.0
68-69	37.1440724135404	38.0	38.0	38.0	36.0	38.0
70-71	37.12893556168402	38.0	38.0	38.0	36.0	38.0
72-73	37.12427737760474	38.0	38.0	38.0	36.0	38.0
74-75	37.08502755055333	38.0	38.0	38.0	36.0	38.0
76-77	37.091023952384504	38.0	38.0	38.0	36.0	38.0
78-79	37.09341061155677	38.0	38.0	38.0	36.0	38.0
80-81	37.08585825730671	38.0	38.0	38.0	36.0	38.0
82-83	37.03975722522725	38.0	38.0	38.0	36.0	38.0
84-85	37.026114552333	38.0	38.0	38.0	36.0	38.0
86-87	36.994640690213615	38.0	38.0	38.0	36.0	38.0
88-89	37.00455551654551	38.0	38.0	38.0	36.0	38.0
90-91	36.99348798778654	38.0	38.0	38.0	36.0	38.0
92-93	36.974453317070726	38.0	38.0	38.0	36.0	38.0
94-95	36.96191470008641	38.0	38.0	38.0	35.5	38.0
96-97	36.945041769052516	38.0	38.0	38.0	35.0	38.0
98-99	36.98029218099053	38.0	38.0	38.0	35.0	38.0
100	35.582828701233716	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	16.0
21	84.0
22	337.0
23	1097.0
24	2531.0
25	5272.0
26	9707.0
27	16568.0
28	26216.0
29	38521.0
30	52152.0
31	67851.0
32	88613.0
33	123992.0
34	200320.0
35	423235.0
36	1498970.0
37	4411970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.9130098061673	18.331622521403805	10.120916512951936	21.63445115947695
2	15.525370608204506	19.85097017508909	40.33015577572384	24.29350344098257
3	20.239758934985215	24.740366405618154	28.926854507733502	26.093020151663133
4	13.459971834721598	16.95357723721927	37.920735351443085	31.665715576616044
5	14.259674842395755	37.24202190413368	34.31614598851919	14.182157264951375
6	31.693422502228934	36.2233855360611	17.99979389883131	14.083398062878654
7	28.043982376851602	32.30142203140413	21.7864356526448	17.86815993909947
8	25.185074830798975	36.033502634822604	20.42621893914734	18.355203595231085
9	26.086365575249033	17.026295983093963	20.92657401873741	35.9607644229196
10-11	25.00800149035831	26.363504190628078	27.872169051182556	20.756325267831052
12-13	25.717120117942688	23.90155683885587	28.227607452480473	22.153715590720967
14-15	22.85491884264147	25.235667213782026	26.014215813758028	25.895198129818475
16-17	22.374427552568715	28.430335795639493	26.764016458240402	22.431220193551386
18-19	22.304940170380792	27.557348080761805	28.533328970188816	21.604382778668587
20-21	23.165893631777042	26.639670726750392	28.562067554006234	21.63236808746633
22-23	22.911289514621693	26.45108826237143	28.45721631387382	22.180405909133054
24-25	22.612485878706885	26.586752468624532	28.58120299780519	22.219558654863395
26-27	22.508432192552394	26.743669457908382	28.56261256951036	22.185285780028867
28-29	22.452373853640147	26.779981723316176	28.606127408008398	22.161517015035276
30-31	22.679037956910044	26.710578560905994	28.470408744590735	22.139974737593224
32-33	22.419339388968123	26.83138327967356	28.46967503786094	22.27960229349738
34-35	22.624079945221816	26.844950568828917	28.438116007525267	22.092853478423997
36-37	22.62090152186054	26.693718472825335	28.396567876062644	22.288812129251482
38-39	22.434850411901607	26.862074384463785	28.526033448204892	22.17704175542972
40-41	22.7274063705564	26.74575232955354	28.34447857877473	22.182362721115332
42-43	22.559278866952553	26.843101666468673	28.194645541568825	22.402973925009952
44-45	22.596763019565785	26.947361259769863	28.111853024045697	22.34402269661865
46-47	22.72733478020548	26.919701933012284	27.8311161039054	22.521847182876833
48-49	22.686421065571793	26.996640240747645	27.827201220588705	22.489737473091864
50-51	22.588953038762643	27.2113845049134	27.730329887687695	22.469332568636265
52-53	22.82366518066136	27.11652494374583	27.581389009057638	22.478420866535174
54-55	22.77923805973269	27.009258557816317	27.636318857262488	22.5751845251885
56-57	22.72741010171456	27.19273063778814	27.58982570413974	22.490033556357556
58-59	22.7302711670432	27.15981139533616	27.63994607131931	22.469971366301333
60-61	22.76566350910902	27.20125058505073	27.635312793661136	22.397773112179113
62-63	22.706034878853703	27.267806836113273	27.629689584278346	22.396468700754678
64-65	22.74072895976751	27.19636027805604	27.59676123763822	22.46614952453824
66-67	22.810726412280854	27.228353508665528	27.464010365154124	22.496909713899495
68-69	22.689300136922053	27.18665454567984	27.612859386896645	22.51118593050147
70-71	22.714520155743738	26.94914345509784	27.713240670284634	22.62309571887379
72-73	22.834557184444222	27.000555303601974	27.555816209622115	22.609071302331685
74-75	22.605730682481585	27.19058687686206	27.53865011643613	22.66503232422022
76-77	22.773583976669904	27.03278437251045	27.513431733251632	22.680199917568018
78-79	22.698441159414536	27.100621169522693	27.440658047701977	22.760279623360795
80-81	22.714999312979295	27.11564209452198	27.459090313576134	22.710268278922598
82-83	22.808413135375307	27.111011394988203	27.531899519506382	22.5486759501301
84-85	22.84022837422483	27.079324000998533	27.440976261976857	22.639471362799778
86-87	22.698412176311802	27.12998109054973	27.516219586448052	22.65538714669041
88-89	22.788799285304254	27.115320367167556	27.44498116355864	22.650899183969553
90-91	22.805079973550004	27.20303057466802	27.39879374367515	22.593095708106826
92-93	22.857610774611913	27.12012879933428	27.424656330512548	22.597604095541257
94-95	22.755379034394938	27.19386636623756	27.43646858552412	22.614286013843376
96-97	22.858772238956586	27.27046591843879	27.46492566906456	22.40583617354006
98-99	23.91782866505716	29.218239210174257	27.63051859497134	19.233413529797243
100	22.93843123869866	30.01993785574713	21.523031261011386	25.518599644542828
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	969.0
1	987.0
2	1344.5
3	1882.0
4	2407.5
5	3331.5
6	4377.0
7	5413.5
8	7202.5
9	8536.5
10	8348.5
11	8265.0
12	9184.0
13	11363.0
14	15283.5
15	19667.5
16	23654.0
17	26910.5
18	27668.0
19	25831.5
20	23128.0
21	20801.0
22	20242.0
23	22481.5
24	27475.5
25	35037.0
26	45198.0
27	57105.0
28	68894.5
29	81958.0
30	95454.5
31	108085.5
32	123266.0
33	140202.5
34	157470.0
35	174054.5
36	192241.5
37	208579.5
38	220024.0
39	231124.5
40	243174.0
41	254362.5
42	262197.5
43	271814.5
44	281777.5
45	288879.0
46	293810.0
47	297419.5
48	301665.0
49	303561.0
50	302339.5
51	298407.5
52	292373.0
53	282383.5
54	267557.0
55	251926.0
56	234499.5
57	215150.0
58	194561.0
59	171712.0
60	147038.0
61	121546.5
62	98118.0
63	77640.0
64	59399.5
65	44566.0
66	33639.5
67	26023.5
68	20195.0
69	15172.5
70	11204.5
71	8080.0
72	5707.5
73	3941.0
74	2664.0
75	1784.0
76	1165.5
77	745.0
78	482.5
79	319.5
80	219.0
81	142.0
82	96.5
83	67.5
84	48.5
85	32.5
86	22.5
87	18.0
88	11.5
89	6.5
90	4.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003674226962740468
3	5.310406157085833E-4
4	1.1481959258563963E-4
5	0.0
6	0.0
7	3.5881122683012386E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.880140869572207E-4
24-25	4.306347843811634E-4
26-27	1.3638788599925228E-4
28-29	0.00290884289677084
30-31	0.002201061026502933
32-33	0.004594458275502904
34-35	0.009525481205089184
36-37	0.0016224417894441607
38-39	0.0
40-41	9.603503121544815E-5
42-43	6.25245632212655E-4
44-45	2.1025355527498388E-4
46-47	4.5505463310082565E-5
48-49	0.004327326646528642
50-51	0.003408557070452633
52-53	4.364243163471534E-4
54-55	0.0027111053239271864
56-57	0.040605236069375085
58-59	0.062491803451835984
60-61	0.030108677063970402
62-63	0.010880792851449406
64-65	9.036460310059025E-4
66-67	1.3265681673220328E-4
68-69	0.0012809591286129013
70-71	0.010400551449075856
72-73	0.016196222054046375
74-75	0.01048569172652213
76-77	0.012325002576769242
78-79	0.0026881746406916375
80-81	0.013765147764113115
82-83	0.04384868475009611
84-85	0.02395295411774085
86-87	0.003453578153651337
88-89	7.383215072685906E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	68.0
20-21	260.0
22-23	566.0
24-25	762.0
26-27	2811.0
28-29	7443.0
30-31	18158.0
32-33	22690.0
34-35	30927.0
36-37	48352.0
38-39	54595.0
40-41	50427.0
42-43	55818.0
44-45	65312.0
46-47	65410.0
48-49	60884.0
50-51	54363.0
52-53	52064.0
54-55	56283.0
56-57	57408.0
58-59	56469.0
60-61	54196.0
62-63	52165.0
64-65	55014.0
66-67	58192.0
68-69	58855.0
70-71	58207.0
72-73	53511.0
74-75	53731.0
76-77	56313.0
78-79	58033.0
80-81	56422.0
82-83	53256.0
84-85	52553.0
86-87	54239.0
88-89	56759.0
90-91	57719.0
92-93	56289.0
94-95	65926.0
96-97	208502.0
98-99	459935.0
100-101	4526565.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.83101590322003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.6309541055977	80.48039787275975
2	6.068590334056964	10.66020908281369
3	1.318212570443003	3.473398477152119
4	0.44236649489628777	1.5541399459315022
5	0.20804038789497345	0.9136199308857738
6	0.10761735710142799	0.5671285081822819
7	0.06480418667215095	0.39842722851378504
8	0.040547516718401004	0.2849063668583967
9	0.028486244457321824	0.22517782109586418
>10	0.08768701434578643	1.2091205840002395
>50	0.002124401646278543	0.12641808177851394
>100	5.447189705134283E-4	0.09026537498351389
>500	2.4667199220778566E-5	0.016790725044622767
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4352449073204954E-5
2	0.0	0.0	0.0	0.0	1.4352449073204954E-5
3	0.0	0.0	0.0	0.0	1.4352449073204954E-5
4	0.0	2.8704898146409907E-5	0.0	1.4352449073204954E-5	1.4352449073204954E-5
5	0.0	2.8704898146409907E-5	0.0	1.4352449073204954E-5	1.4352449073204954E-5
6	2.8704898146409907E-5	2.8704898146409907E-5	0.0	1.4352449073204954E-5	1.4352449073204954E-5
7	2.8704898146409907E-5	4.305734721961486E-5	0.0	1.4352449073204954E-5	1.4352449073204954E-5
8	2.8704898146409907E-5	4.305734721961486E-5	0.0	1.4352449073204954E-5	2.8704898146409907E-5
9	2.8704898146409907E-5	1.1481959258563963E-4	0.0	2.8704898146409907E-5	2.8704898146409907E-5
10-11	2.8704898146409907E-5	1.1481959258563963E-4	0.0	4.305734721961486E-5	2.8704898146409907E-5
12-13	2.8704898146409907E-5	1.2917204165884458E-4	0.0	5.7409796292819815E-5	3.588112268301238E-5
14-15	2.8704898146409907E-5	2.0093428702486934E-4	0.0	7.176224536602476E-5	7.176224536602476E-5
16-17	5.7409796292819815E-5	2.0093428702486934E-4	0.0	7.176224536602476E-5	1.0046714351243467E-4
18-19	5.7409796292819815E-5	2.2246296063467677E-4	0.0	8.611469443922972E-5	1.219958171222421E-4
20-21	5.7409796292819815E-5	2.439916342444842E-4	0.0	8.611469443922972E-5	1.2917204165884458E-4
22-23	5.7409796292819815E-5	2.942252060007016E-4	0.0	1.0046714351243467E-4	1.4352449073204952E-4
24-25	5.7409796292819815E-5	3.372825532203164E-4	0.0	1.0046714351243467E-4	1.7222938887845944E-4
26-27	5.7409796292819815E-5	3.444587777569189E-4	0.0	1.9375806248826688E-4	1.865818379516644E-4
28-29	5.7409796292819815E-5	3.444587777569189E-4	0.0	5.166881666353784E-4	2.0093428702486934E-4
30-31	5.7409796292819815E-5	3.8751612497653375E-4	0.0	0.0017509987869310042	2.0811051156147182E-4
32-33	5.7409796292819815E-5	3.8751612497653375E-4	0.0	0.0042555011502052684	2.2963918517127926E-4
34-35	5.7409796292819815E-5	4.736308194157635E-4	0.0	0.007757498724067278	2.2963918517127926E-4
36-37	5.7409796292819815E-5	5.597455138549932E-4	0.0	0.012730622327932792	2.2963918517127926E-4
38-39	5.7409796292819815E-5	5.597455138549932E-4	0.0	0.019713088802047005	2.439916342444842E-4
40-41	8.611469443922972E-5	6.31507759221018E-4	0.0	0.026702731500697817	2.5834408331768915E-4
42-43	8.611469443922972E-5	6.458602082942229E-4	0.0	0.03310392378734722	2.6552030785429164E-4
44-45	8.611469443922972E-5	6.745651064406328E-4	0.0	0.040933184756780525	2.726965323908941E-4
46-47	8.611469443922972E-5	6.889175555138378E-4	0.0	0.04830316735587127	2.726965323908941E-4
48-49	8.611469443922972E-5	6.889175555138378E-4	0.0	0.055996080059109124	2.726965323908941E-4
50-51	8.611469443922972E-5	7.822084744896699E-4	0.0	0.0634665298017123	2.726965323908941E-4
52-53	8.611469443922972E-5	7.893846990262725E-4	0.0	0.07080780750265664	2.942252060007016E-4
54-55	8.611469443922972E-5	7.893846990262725E-4	0.0	0.07722335223837926	3.15753879610509E-4
56-57	8.611469443922972E-5	8.037371480994773E-4	0.0	0.0843421669786889	3.3010632868371397E-4
58-59	8.611469443922972E-5	8.109133726360799E-4	0.0	0.09149686284168157	3.444587777569189E-4
60-61	9.32909189758322E-5	8.539707198556947E-4	0.0	0.09847215309125919	3.444587777569189E-4
62-63	1.0046714351243467E-4	8.970280670753095E-4	0.0	0.10450735792654187	3.731636759033288E-4
64-65	1.0046714351243467E-4	0.0010262001087341542	0.0	0.1104133907201657	3.731636759033288E-4
66-67	1.0046714351243467E-4	0.0010333763332707566	0.0	0.1164485955554484	3.8033990043993127E-4
68-69	1.0046714351243467E-4	0.0010549050068805641	0.0	0.12214651783751077	3.946923495131362E-4
70-71	1.0046714351243467E-4	0.001069257455953769	0.0	0.12789467369132934	4.1622102312294365E-4
72-73	1.0046714351243467E-4	0.001083609905026974	0.0	0.13211429371885158	4.592783703425585E-4
74-75	1.0046714351243467E-4	0.0011338434767831913	0.0	0.13540818078115213	4.592783703425585E-4
76-77	1.0764336804903714E-4	0.0012127819466858187	0.0	0.1382427894731101	4.592783703425585E-4
78-79	1.1481959258563963E-4	0.001234310620295626	0.0	0.14032389458872482	4.592783703425585E-4
80-81	1.1481959258563963E-4	0.001234310620295626	0.0	0.14096975479701906	4.808070439523659E-4
82-83	1.1481959258563963E-4	0.001234310620295626	0.0	0.14144338561643482	5.023357175621733E-4
84-85	1.1481959258563963E-4	0.001248663069368831	0.0	0.1416227912298499	5.023357175621733E-4
86-87	1.1481959258563963E-4	0.001248663069368831	0.0	0.1417232583733623	5.095119420987758E-4
88	1.1481959258563963E-4	0.0013204253147348557	0.0	0.1417304345978989	5.310406157085833E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10130	0.0	45.82646	1
GTATCAA	18160	0.0	36.77141	1
TCAACGC	20515	0.0	31.99241	4
ATCAACG	20845	0.0	31.404732	3
TATCAAC	20905	0.0	31.396467	2
CAACGCA	21215	0.0	30.876963	5
AACGCAG	22035	0.0	29.747128	6
ACGCAGA	26115	0.0	25.099672	7
CGCAGAG	26780	0.0	24.476223	8
GCAGAGT	30705	0.0	21.526602	9
GTACATG	23800	0.0	21.354292	1
TACATGG	23795	0.0	20.700766	2
ACATGGG	23640	0.0	20.245188	3
GAGTACT	16735	0.0	20.190763	12-13
GTGGTAT	5470	0.0	19.959585	1
TGGTATC	5315	0.0	18.790049	2
CAGAGTA	29675	0.0	18.715961	10-11
GTACTTT	18235	0.0	18.402582	14-15
CATGGGG	16705	0.0	17.884008	4
GATCAAC	3425	0.0	17.791847	1
>>END_MODULE