FastQCFastQC Report
Fri 27 May 2016
SRR2139578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6635350
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131100.053.742231
GTATCAA240950.038.3743631
TATCAAC284300.032.166472
TCAACGC283500.032.062824
ATCAACG285750.031.7955743
CAACGCA290000.031.3148085
GTGGTAT55350.030.9835191
AACGCAG301050.030.193466
TGGTATC55350.029.7624932
ACGCAGA347900.026.1517377
CGCAGAG353500.025.7374528
GATCAAC41100.023.1240021
GCAGAGT396300.023.0324389
GCAACGC8300.021.8832951
GTACATG337900.021.4761941
GAGTACT212200.021.30838612-13
GGACCGA11200.021.1192876
ACCGAAC9450.020.5605778
TACATGG341750.020.505022
CAGAGTA395050.020.33075510-11
Produced by FastQC (version 0.11.5)