##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139578_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6635350
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.268554032567987	25.0	18.0	33.0	18.0	33.0
2	27.370383325672346	29.0	25.0	31.0	18.0	33.0
3	26.788368661788752	29.0	25.0	31.0	18.0	33.0
4	30.954583857671413	31.0	30.0	33.0	28.0	33.0
5	31.762353153940637	33.0	31.0	33.0	29.0	33.0
6	36.10715259933538	38.0	36.0	38.0	33.0	38.0
7	36.419323019885915	38.0	37.0	38.0	34.0	38.0
8	36.53649453306909	38.0	37.0	38.0	34.0	38.0
9	37.201499091984594	38.0	38.0	38.0	36.0	38.0
10-11	37.244291936371106	38.0	38.0	38.0	36.0	38.0
12-13	37.34374772996149	38.0	38.0	38.0	36.5	38.0
14-15	37.326822247507664	38.0	38.0	38.0	36.0	38.0
16-17	37.439227923169085	38.0	38.0	38.0	37.0	38.0
18-19	37.46458566616681	38.0	38.0	38.0	37.0	38.0
20-21	37.49582378134588	38.0	38.0	38.0	37.0	38.0
22-23	37.519328715206555	38.0	38.0	38.0	37.0	38.0
24-25	37.528398703352465	38.0	38.0	38.0	37.0	38.0
26-27	37.49960935244948	38.0	38.0	38.0	37.0	38.0
28-29	37.47852939556176	38.0	38.0	38.0	37.0	38.0
30-31	37.489797653337064	38.0	38.0	38.0	37.0	38.0
32-33	37.477962713950006	38.0	38.0	38.0	37.0	38.0
34-35	37.44541258817003	38.0	38.0	38.0	37.0	38.0
36-37	37.4245709316596	38.0	38.0	38.0	37.0	38.0
38-39	37.412258685481596	38.0	38.0	38.0	37.0	38.0
40-41	37.404531822957665	38.0	38.0	38.0	37.0	38.0
42-43	37.37045981523296	38.0	38.0	38.0	37.0	38.0
44-45	37.32660871123449	38.0	38.0	38.0	37.0	38.0
46-47	37.273684482604	38.0	38.0	38.0	37.0	38.0
48-49	37.20423756116432	38.0	38.0	38.0	37.0	38.0
50-51	37.1209152017086	38.0	38.0	38.0	37.0	38.0
52-53	37.14999155267506	38.0	38.0	38.0	37.0	38.0
54-55	37.13339341086758	38.0	38.0	38.0	36.5	38.0
56-57	37.08898604703445	38.0	38.0	38.0	36.0	38.0
58-59	37.07869368054534	38.0	38.0	38.0	36.0	38.0
60-61	37.09965579204819	38.0	38.0	38.0	36.0	38.0
62-63	37.10599210010959	38.0	38.0	38.0	36.0	38.0
64-65	37.05396897595269	38.0	38.0	38.0	36.0	38.0
66-67	37.099183457088955	38.0	38.0	38.0	36.0	38.0
68-69	37.11434226068408	38.0	38.0	38.0	36.0	38.0
70-71	37.10007293018809	38.0	38.0	38.0	36.0	38.0
72-73	37.10092699681007	38.0	38.0	38.0	36.0	38.0
74-75	37.06602020489399	38.0	38.0	38.0	36.0	38.0
76-77	37.076436943222106	38.0	38.0	38.0	36.0	38.0
78-79	37.081303538143445	38.0	38.0	38.0	36.0	38.0
80-81	37.07807393473678	38.0	38.0	38.0	36.0	38.0
82-83	37.03632245969956	38.0	38.0	38.0	36.0	38.0
84-85	37.02557832744861	38.0	38.0	38.0	36.0	38.0
86-87	36.99801600365449	38.0	38.0	38.0	36.0	38.0
88-89	37.00880482650956	38.0	38.0	38.0	36.0	38.0
90-91	37.00077297455664	38.0	38.0	38.0	36.0	38.0
92-93	36.987525313193814	38.0	38.0	38.0	36.0	38.0
94-95	36.97415241430585	38.0	38.0	38.0	35.5	38.0
96-97	36.95884697396676	38.0	38.0	38.0	35.0	38.0
98-99	36.99413348476605	38.0	38.0	38.0	35.0	38.0
100	35.543416326571354	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	19.0
21	91.0
22	288.0
23	939.0
24	2358.0
25	4980.0
26	9192.0
27	16077.0
28	26624.0
29	39318.0
30	52048.0
31	66606.0
32	85241.0
33	117282.0
34	186434.0
35	399350.0
36	1449249.0
37	4179251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.70798827492145	19.241185468739403	10.514893713217841	20.535932543121312
2	14.999896761459741	20.315174452786756	41.528795489576	23.156133296177504
3	19.543463543817406	26.49965909645792	29.606342312971755	24.350535046752917
4	12.75494902601914	17.93752729699401	39.179283190356614	30.128240486630236
5	13.320548275524274	38.3997226973709	34.69800387319433	13.581725153910496
6	29.717724008530073	37.55648157218534	18.806257394108826	13.919537025175762
7	26.94787134270428	33.53876889072485	22.37473236083253	17.13862740573834
8	24.662723141959354	36.51759138553354	20.943401629152948	17.87628384335416
9	26.319003519030648	17.160662210734927	21.415328505655314	35.10500576457911
10-11	24.27593118674976	27.188626070968375	28.250499220086354	20.284943522195515
12-13	25.266361231886787	24.93161626741619	28.73458822820198	21.067434272495046
14-15	22.52502882289555	26.05170789785015	26.80810356650365	24.615159712750646
16-17	21.351835246068408	29.45956882455334	27.586125826067953	21.6024701033103
18-19	21.31791088638881	28.203124175815898	29.81700287098646	20.661962066808833
20-21	22.454261503856284	27.115274046667242	29.803625320728983	20.62683912874749
22-23	22.158881405599438	27.09384340374699	29.717156978995725	21.03011821165785
24-25	21.765071173197157	27.30870507587587	29.872997594400196	21.053226156526772
26-27	21.57826257825489	27.41674180171252	29.844115067947158	21.16088055208543
28-29	21.36007751859064	27.26939195884136	30.048755296164803	21.321775226403197
30-31	21.610454950944746	27.225308294924925	30.04309207845971	21.12114467567062
32-33	21.332417492974848	27.394514437281366	29.95393086768151	21.319137202062276
34-35	21.532677161675306	27.417906811245967	29.93165969623881	21.117756330839914
36-37	21.54892750290674	27.314379026587787	29.850292429805016	21.286401040700458
38-39	21.38628027836626	27.397564545090745	29.9439938400528	21.272161336490193
40-41	21.55028984121607	27.298356623781007	29.946116450017286	21.205237084985637
42-43	21.625207954508284	27.302554361695293	29.65594638456387	21.416291299232554
44-45	21.54013886082212	27.543717091983915	29.493788497525653	21.422355549668314
46-47	21.722434921652635	27.541856327747716	29.120412596845608	21.615296153754038
48-49	21.73177940958583	27.65281961205287	28.947678355502788	21.667722622858513
50-51	21.675560285834738	27.82196477096175	28.826638739444615	21.675836203758895
52-53	21.76549477009978	27.766639148341664	28.89059141703225	21.577274664526307
54-55	21.798616514841495	27.859225075703563	28.719928455274356	21.622229954180582
56-57	21.82535902696366	28.001313878823403	28.55395062879676	21.619376465416178
58-59	21.642318608973113	28.017390693420875	28.777405746485613	21.562884951120402
60-61	21.799069495048588	28.22879275210247	28.549329402805178	21.42280835004376
62-63	21.674784494610794	28.315161765992563	28.609581098183085	21.400472641213558
64-65	21.676772137530353	28.166897871970676	28.58641534447347	21.5699146460255
66-67	21.798770923783472	28.161973019019975	28.529176182230366	21.51007987496619
68-69	21.77485615312715	28.02485285075626	28.656054813368492	21.544236182748097
70-71	21.736021492834563	27.819594528779362	28.817379464741233	21.62700451364484
72-73	21.851491628927263	27.813885075646215	28.699613443399535	21.635009852026982
74-75	21.696645683414026	27.96986485641903	28.656810812067867	21.676678648099077
76-77	21.77678355499596	27.780213709189656	28.72670222158473	21.716300514229655
78-79	21.748152475926315	27.75841649341232	28.641284268307853	21.852146762353513
80-81	21.754278011147193	27.92772014939553	28.51365636968711	21.80434546977017
82-83	21.90166527444438	27.808257407552812	28.606970445470136	21.68310687253267
84-85	21.84512506364231	27.81755518221494	28.526528826491703	21.810790927651052
86-87	21.771617761995916	27.932665226938457	28.555252221445087	21.740464789620535
88-89	21.89796649508285	27.834712633959956	28.507992255058635	21.75932861589856
90-91	21.926486069163285	27.87234817785857	28.47601284514432	21.725152907833824
92-93	21.931590178284825	27.955831832558985	28.3942159187487	21.71836207040749
94-95	21.80642024129766	27.894122195196598	28.480966398737134	21.81849116476861
96-97	21.991084239767595	27.99676273721648	28.458138099635168	21.55401492338076
98-99	22.93428215775127	29.938317751658705	28.74707217408786	18.38032791650216
100	21.946669124189473	30.70767603690091	22.76001941566824	24.585635423241374
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1617.0
1	1559.0
2	1973.0
3	2655.0
4	3237.5
5	4422.0
6	5905.5
7	7596.0
8	10753.0
9	13071.0
10	12351.5
11	11322.0
12	11977.0
13	14692.0
14	20085.0
15	26214.5
16	31697.5
17	36079.5
18	36591.5
19	33561.0
20	29392.5
21	25557.0
22	24085.5
23	26376.0
24	31516.0
25	39435.5
26	50613.0
27	63736.5
28	77067.0
29	92413.5
30	110096.0
31	126892.5
32	145564.5
33	166405.5
34	186630.5
35	205673.5
36	225135.0
37	240528.0
38	250289.0
39	258232.5
40	264650.0
41	267913.5
42	268320.5
43	273898.5
44	282081.5
45	288906.5
46	287946.5
47	281882.0
48	277331.5
49	270546.0
50	261420.5
51	250701.0
52	239350.0
53	225191.0
54	208454.0
55	192060.5
56	175243.5
57	156008.0
58	138119.5
59	120863.5
60	102742.5
61	85132.0
62	68344.5
63	53399.0
64	41116.0
65	31107.5
66	22369.0
67	16466.5
68	12696.5
69	9499.0
70	7010.5
71	5151.5
72	3718.0
73	2606.0
74	1813.5
75	1299.5
76	872.5
77	502.0
78	326.0
79	248.0
80	184.0
81	136.5
82	110.5
83	85.5
84	60.5
85	39.5
86	26.0
87	18.5
88	14.5
89	12.0
90	8.0
91	4.5
92	2.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0034813536588122703
3	6.32973392511322E-4
4	6.028318023917352E-5
5	0.0
6	0.0
7	2.5620351601648745E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.5214528241785596E-4
24-25	3.617430225799995E-4
26-27	1.1306046616940663E-4
28-29	0.0023226682390426806
30-31	0.0018425728418506892
32-33	0.004376095916754239
34-35	0.008857707462740083
36-37	0.0012978050299869302
38-39	0.0
40-41	1.239662570046746E-4
42-43	5.776751805839938E-4
44-45	2.047579446082508E-4
46-47	7.956833224934742E-6
48-49	0.004051620539690326
50-51	0.003359593615765875
52-53	4.910065601749816E-4
54-55	0.002615449401862398
56-57	0.04058221327201502
58-59	0.06270417069240991
60-61	0.029705816644681818
62-63	0.010732529793246152
64-65	8.54231370378673E-4
66-67	2.3527437305261443E-4
68-69	0.0015322766143238626
70-71	0.011306428425678663
72-73	0.016738263237806444
74-75	0.010309485298719381
76-77	0.012471065523253356
78-79	0.0027692759667783205
80-81	0.013963398301818666
82-83	0.04448086462328282
84-85	0.024233357493487883
86-87	0.003798763492841548
88-89	5.844353185951734E-5
90-91	1.9695096275539123E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	45.0
20-21	183.0
22-23	479.0
24-25	703.0
26-27	2313.0
28-29	6535.0
30-31	16351.0
32-33	20625.0
34-35	28318.0
36-37	43662.0
38-39	50965.0
40-41	47702.0
42-43	53078.0
44-45	63882.0
46-47	65311.0
48-49	60278.0
50-51	53039.0
52-53	48942.0
54-55	51963.0
56-57	54029.0
58-59	53813.0
60-61	53558.0
62-63	51689.0
64-65	55431.0
66-67	59332.0
68-69	60564.0
70-71	63120.0
72-73	52094.0
74-75	51608.0
76-77	53445.0
78-79	55670.0
80-81	55803.0
82-83	51670.0
84-85	50571.0
86-87	51982.0
88-89	54986.0
90-91	56092.0
92-93	54662.0
94-95	61873.0
96-97	188298.0
98-99	422825.0
100-101	4307861.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.68892981918499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.623530250993	78.5605685389512
2	6.721372374576082	11.653371561364697
3	1.5590760210387453	4.05463895311806
4	0.5251500777570423	1.8209879294087912
5	0.23204600637460535	1.0057909980715158
6	0.11842108166125365	0.6159478102346636
7	0.06518104719847044	0.39553326582904313
8	0.04047628080377878	0.2807076372752323
9	0.024400523500135357	0.19037297423291472
>10	0.08802932436870892	1.157785198216886
>50	0.0016041692538267093	0.09174068172722609
>100	6.327965932304995E-4	0.11620902643564521
>500	8.004588111188995E-5	0.05634542513409246
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.014159011958676E-5	0.0	0.0	0.0
3	0.0	3.014159011958676E-5	0.0	4.521238517938014E-5	0.0
4	0.0	9.042477035876028E-5	0.0	4.521238517938014E-5	0.0
5	0.0	9.042477035876028E-5	0.0	6.028318023917352E-5	0.0
6	1.507079505979338E-5	9.042477035876028E-5	0.0	6.028318023917352E-5	0.0
7	1.507079505979338E-5	9.042477035876028E-5	0.0	6.028318023917352E-5	1.507079505979338E-5
8	1.507079505979338E-5	9.042477035876028E-5	0.0	6.028318023917352E-5	3.014159011958676E-5
9	1.507079505979338E-5	2.2606192589690068E-4	0.0	7.53539752989669E-5	3.014159011958676E-5
10-11	3.014159011958676E-5	2.4113272095669408E-4	0.0	1.2810175800824372E-4	3.014159011958676E-5
12-13	3.767698764948345E-5	2.4113272095669408E-4	0.0	1.8084954071752055E-4	3.014159011958676E-5
14-15	4.521238517938014E-5	3.5416368390514443E-4	0.0	2.486681184865908E-4	5.274778270927683E-5
16-17	4.521238517938014E-5	3.616990814350411E-4	0.0	3.466282863752477E-4	6.028318023917352E-5
18-19	4.521238517938014E-5	3.9937606908452457E-4	0.0	3.616990814350411E-4	9.042477035876028E-5
20-21	5.274778270927683E-5	4.5212385179380137E-4	0.0	3.767698764948345E-4	1.3563715553814042E-4
22-23	6.028318023917352E-5	4.747300443834915E-4	0.0	4.2198226167421463E-4	1.3563715553814042E-4
24-25	6.028318023917352E-5	5.500840196824583E-4	0.0	4.521238517938014E-4	1.3563715553814042E-4
26-27	6.028318023917352E-5	5.877610073319418E-4	0.0	5.199424295628716E-4	1.3563715553814042E-4
28-29	6.028318023917352E-5	5.877610073319418E-4	0.0	9.343892937071895E-4	1.3563715553814042E-4
30-31	6.028318023917352E-5	6.932565727504954E-4	0.0	0.0020722343207215895	1.3563715553814042E-4
32-33	6.028318023917352E-5	6.932565727504954E-4	0.0	0.00423489341180194	1.3563715553814042E-4
34-35	6.028318023917352E-5	8.288937282886358E-4	0.0	0.006849676354676091	1.3563715553814042E-4
36-37	6.028318023917352E-5	9.796016788865697E-4	0.0	0.011288025499785241	1.3563715553814042E-4
38-39	6.781857776907021E-5	0.0010248140640659498	0.0	0.01736155590888197	1.3563715553814042E-4
40-41	7.53539752989669E-5	0.0011453804245442967	0.0	0.02426398004626734	1.3563715553814042E-4
42-43	8.288937282886359E-5	0.0011755220146638836	0.0	0.03020940869735583	1.3563715553814042E-4
44-45	9.042477035876028E-5	0.0012282697973731605	0.0	0.036403505466930906	1.3563715553814042E-4
46-47	1.0549556541855366E-4	0.001265946785022644	0.0	0.04280859336734309	1.3563715553814042E-4
48-49	1.0549556541855366E-4	0.001265946785022644	0.0	0.04960552193930991	1.3563715553814042E-4
50-51	1.0549556541855366E-4	0.0014015839405607843	0.0	0.05613871159773034	1.3563715553814042E-4
52-53	1.2056636047834704E-4	0.0014166547356205777	0.0	0.06216702962164769	1.507079505979338E-4
54-55	1.2056636047834704E-4	0.0014317255306803712	0.0	0.0683234494035733	1.507079505979338E-4
56-57	1.2056636047834704E-4	0.0014920087109195446	0.0	0.07429901964478136	1.507079505979338E-4
58-59	1.2810175800824372E-4	0.0014920087109195446	0.0	0.08054586419706572	1.582433481278305E-4
60-61	1.3563715553814042E-4	0.0015146149035092345	0.0	0.0867399609666408	1.6577874565772718E-4
62-63	1.3563715553814042E-4	0.001529685698569028	0.0	0.09228601354864477	1.8084954071752055E-4
64-65	1.3563715553814042E-4	0.0017331414318762387	0.0	0.097666287384991	1.8084954071752055E-4
66-67	1.4317255306803712E-4	0.0017331414318762387	0.0	0.10328769394229392	1.8084954071752055E-4
68-69	1.507079505979338E-4	0.0017406768294061354	0.0	0.10871318016381953	1.8084954071752055E-4
70-71	1.507079505979338E-4	0.0017557476244659288	0.0	0.11392767525450806	1.8084954071752055E-4
72-73	1.507079505979338E-4	0.0017632830219958253	0.0	0.11811735628113061	1.8084954071752055E-4
74-75	1.507079505979338E-4	0.0018160308047051022	0.0	0.12172681169795113	1.8084954071752055E-4
76-77	1.507079505979338E-4	0.0019139909725937592	0.0	0.12476357690249949	1.9592033577731392E-4
78-79	1.507079505979338E-4	0.0019441325627133459	0.0	0.1265419307195551	1.9592033577731392E-4
80-81	1.507079505979338E-4	0.0019516679602432426	0.0	0.12716736871453654	1.9592033577731392E-4
82-83	1.507079505979338E-4	0.0019592033577731393	0.0	0.12763456336139012	1.9592033577731392E-4
84-85	1.6577874565772718E-4	0.0019592033577731393	0.0	0.12782294829963753	1.9592033577731392E-4
86-87	1.6577874565772718E-4	0.0019592033577731393	0.0	0.12792090846752618	1.9592033577731392E-4
88	1.6577874565772718E-4	0.002034557333072106	0.0	0.12793597926258599	1.9592033577731392E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13110	0.0	53.74223	1
GTATCAA	24095	0.0	38.374363	1
TATCAAC	28430	0.0	32.16647	2
TCAACGC	28350	0.0	32.06282	4
ATCAACG	28575	0.0	31.795574	3
CAACGCA	29000	0.0	31.314808	5
GTGGTAT	5535	0.0	30.983519	1
AACGCAG	30105	0.0	30.19346	6
TGGTATC	5535	0.0	29.762493	2
ACGCAGA	34790	0.0	26.151737	7
CGCAGAG	35350	0.0	25.737452	8
GATCAAC	4110	0.0	23.124002	1
GCAGAGT	39630	0.0	23.032438	9
GCAACGC	830	0.0	21.883295	1
GTACATG	33790	0.0	21.476194	1
GAGTACT	21220	0.0	21.308386	12-13
GGACCGA	1120	0.0	21.119287	6
ACCGAAC	945	0.0	20.560577	8
TACATGG	34175	0.0	20.50502	2
CAGAGTA	39505	0.0	20.330755	10-11
>>END_MODULE