FastQCFastQC Report
Fri 27 May 2016
SRR2139577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5646079
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104700.046.7568741
GTATCAA195750.037.0467221
TCAACGC226300.031.366914
TATCAAC228750.031.3214152
ATCAACG229400.030.9068643
CAACGCA230250.030.8288025
AACGCAG239050.029.659216
ACGCAGA277250.025.5577497
GTGGTAT54950.025.2922381
CGCAGAG281700.025.124568
TGGTATC52450.024.8367232
GCAGAGT319050.022.261349
GTACATG261200.021.9028821
TACATGG261850.021.4524172
ACATGGG264600.020.7745843
GATCAAC36250.020.4859051
GAGTACT172650.020.25663412-13
CAGAGTA314950.018.84310710-11
CATGGGG165300.018.6726684
GTACTTT194150.017.99254214-15
Produced by FastQC (version 0.11.5)