##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5646079
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.266555072998447	25.0	18.0	33.0	18.0	33.0
2	27.285778856441787	28.0	25.0	31.0	18.0	33.0
3	26.73481277892144	29.0	25.0	31.0	18.0	33.0
4	30.943975633355468	31.0	30.0	33.0	28.0	33.0
5	31.746925432676377	33.0	31.0	33.0	29.0	33.0
6	36.04904500982009	38.0	36.0	38.0	33.0	38.0
7	36.437846158369375	38.0	37.0	38.0	34.0	38.0
8	36.52439347731408	38.0	37.0	38.0	34.0	38.0
9	37.20449802420406	38.0	38.0	38.0	36.0	38.0
10-11	37.25076083774244	38.0	38.0	38.0	36.0	38.0
12-13	37.35137367011691	38.0	38.0	38.0	37.0	38.0
14-15	37.3279438704276	38.0	38.0	38.0	36.0	38.0
16-17	37.43328467419602	38.0	38.0	38.0	37.0	38.0
18-19	37.461209805955605	38.0	38.0	38.0	37.0	38.0
20-21	37.492340250208585	38.0	38.0	38.0	37.0	38.0
22-23	37.51381941964921	38.0	38.0	38.0	37.0	38.0
24-25	37.522940794735746	38.0	38.0	38.0	37.0	38.0
26-27	37.49693564989403	38.0	38.0	38.0	37.0	38.0
28-29	37.47499655796658	38.0	38.0	38.0	37.0	38.0
30-31	37.48451371188277	38.0	38.0	38.0	37.0	38.0
32-33	37.47062834813602	38.0	38.0	38.0	37.0	38.0
34-35	37.436930153772025	38.0	38.0	38.0	37.0	38.0
36-37	37.415917656370134	38.0	38.0	38.0	37.0	38.0
38-39	37.40518647040158	38.0	38.0	38.0	37.0	38.0
40-41	37.39003678459376	38.0	38.0	38.0	37.0	38.0
42-43	37.35692009504007	38.0	38.0	38.0	37.0	38.0
44-45	37.3155091923031	38.0	38.0	38.0	37.0	38.0
46-47	37.264932970115055	38.0	38.0	38.0	37.0	38.0
48-49	37.200457096135224	38.0	38.0	38.0	37.0	38.0
50-51	37.11874706156942	38.0	38.0	38.0	37.0	38.0
52-53	37.14871322468819	38.0	38.0	38.0	37.0	38.0
54-55	37.1308538192081	38.0	38.0	38.0	36.5	38.0
56-57	37.08232504271086	38.0	38.0	38.0	36.0	38.0
58-59	37.07228122356078	38.0	38.0	38.0	36.0	38.0
60-61	37.09857598745718	38.0	38.0	38.0	36.0	38.0
62-63	37.102599212713955	38.0	38.0	38.0	36.0	38.0
64-65	37.04638903065752	38.0	38.0	38.0	36.0	38.0
66-67	37.09010864892865	38.0	38.0	38.0	36.0	38.0
68-69	37.10544044950815	38.0	38.0	38.0	36.0	38.0
70-71	37.08984001032708	38.0	38.0	38.0	36.0	38.0
72-73	37.08666247951673	38.0	38.0	38.0	36.0	38.0
74-75	37.04924947353737	38.0	38.0	38.0	36.0	38.0
76-77	37.06002261134803	38.0	38.0	38.0	36.0	38.0
78-79	37.06059839527539	38.0	38.0	38.0	36.0	38.0
80-81	37.057963676076305	38.0	38.0	38.0	36.0	38.0
82-83	37.013537336892384	38.0	38.0	38.0	36.0	38.0
84-85	37.00002008473405	38.0	38.0	38.0	36.0	38.0
86-87	36.974760310736926	38.0	38.0	38.0	36.0	38.0
88-89	36.98175949717366	38.0	38.0	38.0	36.0	38.0
90-91	36.97016013756581	38.0	38.0	38.0	35.5	38.0
92-93	36.954719280521	38.0	38.0	38.0	35.0	38.0
94-95	36.942931887948696	38.0	38.0	38.0	35.0	38.0
96-97	36.92707804412489	38.0	38.0	38.0	35.0	38.0
98-99	36.95991762041254	38.0	38.0	38.0	35.0	38.0
100	35.59553445291165	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	60.0
22	293.0
23	789.0
24	2070.0
25	4198.0
26	8198.0
27	14275.0
28	23265.0
29	33764.0
30	43788.0
31	56222.0
32	71982.0
33	99631.0
34	159066.0
35	344511.0
36	1299078.0
37	3484877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.052222081908525	18.91714586352759	10.42766139120618	20.602970663357702
2	15.167166054453904	19.957693290704267	41.62703180158816	23.248108853253672
3	20.190175836577794	26.049080789041803	28.94884873250942	24.811894641870992
4	13.218811298114177	17.19493630530944	38.655189667158155	30.931062729418223
5	13.673028662900395	38.020190649121275	34.335970148487114	13.970810539491211
6	30.44709434635966	37.058514413276896	18.318624305469335	14.17576693489411
7	27.127192104650717	33.09310673039491	22.59170911811391	17.18799204684047
8	24.463313389699294	36.933595863607295	20.74108421083021	17.862006535863205
9	26.007872011709367	17.310526473327773	21.698545131940236	34.98305638302263
10-11	24.809881335347946	27.010222492458922	27.842463769989756	20.33743240220337
12-13	25.399768582763365	24.63250159978279	28.60726886747422	21.360460949979622
14-15	22.647734826239592	25.734230782105598	26.60717287165128	25.01086152000353
16-17	21.664123013510793	28.98145775147673	27.40183940040513	21.952579834607345
18-19	21.647970210831268	27.853179170890098	29.55925696399218	20.939593654286455
20-21	22.5929407913975	26.885316951668504	29.534286919187025	20.98745533774697
22-23	22.439024174235875	26.712167533406074	29.457124739623925	21.391683552734126
24-25	22.009904380357113	26.88841221863824	29.590124600123396	21.511558800881247
26-27	21.858580339420154	26.99277591407954	29.600973097634785	21.547670648865523
28-29	21.73229630686741	27.032805143589815	29.649957031861128	21.584941517681642
30-31	21.956685259620663	26.930809493131875	29.55568510406775	21.556820143179714
32-33	21.721241600150506	27.14656584917247	29.465502426756807	21.666690123920215
34-35	21.953251826707117	27.176161798608334	29.486037019689903	21.384549354994643
36-37	21.96404746996682	26.994930592838255	29.365171353233134	21.675850583961797
38-39	21.814844992624664	27.15248979138229	29.468734218567473	21.56393099742558
40-41	21.956345172731865	27.05320701960396	29.41437870410006	21.576069103564123
42-43	21.939299290607682	27.13525773359948	29.152603410675574	21.77283956511726
44-45	21.913206912028727	27.312294284859366	28.98769449431478	21.786804308797127
46-47	22.061529691204207	27.364247528513765	28.67294174713258	21.901281033149452
48-49	22.125353817239652	27.486040168780058	28.436273702293192	21.952332311687105
50-51	22.019219856169467	27.62322948674197	28.362881181488763	21.9946694755998
52-53	22.176293031284175	27.562824377630633	28.232213507424763	22.028669083660425
54-55	22.178421066265432	27.631073401975176	28.184654714161617	22.005850817597775
56-57	22.14385596949824	27.645230885893184	28.19365804131417	22.01725510329441
58-59	22.091263182380487	27.58831799401065	28.284776923981315	22.035641899627546
60-61	22.133399952589976	27.65019985776993	28.3128979785347	21.903502211105387
62-63	22.124985779802593	27.675859194134446	28.29744957275404	21.901705453308924
64-65	22.09334567884112	27.611773059422678	28.28444959269314	22.01043166904306
66-67	22.197128700045816	27.592834408717298	28.180769063344556	22.02926782789233
68-69	22.09118095366446	27.562537157225925	28.31276127340639	22.03352061570323
70-71	22.06916538888524	27.430981190474725	28.381594466216452	22.118258954423585
72-73	22.226887491175766	27.488455915063824	28.288268799821182	21.996387793939228
74-75	22.01025609120879	27.669770541800386	28.225010981262688	22.09496238572813
76-77	22.172043459294915	27.460038638267097	28.243366430125043	22.12455147231294
78-79	22.092363327186973	27.456590743940545	28.214794803020986	22.236251125851492
80-81	22.11876589197802	27.628281971572342	28.094897921763113	22.15805421468652
82-83	22.217652592541363	27.476222055320225	28.256298706360454	22.04982664577796
84-85	22.21275340850984	27.493264481233798	28.104322577122144	22.189659533134215
86-87	22.110232143268803	27.636002097227912	28.13911217745811	22.114653582045175
88-89	22.196556174390565	27.541847926732572	28.112987607721234	22.148608291155625
90-91	22.27683239817143	27.564189472028804	28.059461200867347	22.09951692893242
92-93	22.258339622178454	27.63871965248762	28.03995410676925	22.06298661856467
94-95	22.055348572083293	27.61207708691098	28.119913911555173	22.21266042945056
96-97	22.289913553625595	27.739265777527784	28.05478664217094	21.916034026675682
98-99	23.230584898258556	29.635559606241674	28.286629313268257	18.847226182231513
100	29.666864311691587	40.5566437567628	29.776491931545607	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1811.0
1	1726.0
2	1996.0
3	2594.0
4	3242.0
5	4635.0
6	6116.5
7	7448.5
8	9528.5
9	10530.0
10	9616.5
11	8834.0
12	9397.5
13	11738.5
14	16304.0
15	21541.5
16	26357.5
17	30748.0
18	32069.5
19	29579.5
20	25944.0
21	22935.0
22	21946.0
23	24165.0
24	29155.5
25	36585.0
26	46955.5
27	58584.5
28	70332.5
29	83418.0
30	97023.5
31	109986.5
32	124904.5
33	141190.5
34	154472.0
35	167539.0
36	182333.5
37	193464.0
38	201828.0
39	208951.5
40	214091.0
41	217330.5
42	219935.5
43	224713.0
44	230894.5
45	235906.0
46	237077.0
47	237314.5
48	237803.5
49	235920.5
50	232071.5
51	225980.5
52	218106.0
53	208515.0
54	196578.0
55	184170.0
56	169496.0
57	153312.0
58	137762.5
59	120073.0
60	102559.5
61	85753.0
62	69910.5
63	55744.5
64	43197.0
65	32905.0
66	24422.0
67	19027.0
68	14838.5
69	11058.0
70	8228.5
71	6167.0
72	4635.0
73	3418.0
74	2501.5
75	1794.0
76	1215.5
77	734.0
78	492.0
79	387.0
80	279.0
81	182.5
82	140.0
83	113.5
84	82.0
85	55.5
86	38.5
87	26.5
88	18.0
89	10.5
90	5.5
91	3.5
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0029400934701763826
3	7.261676643206728E-4
4	7.084562578738272E-5
5	0.0
6	0.0
7	3.7193953538375925E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.668018489076311E-4
24-25	3.5428334767295184E-4
26-27	9.744431898734447E-5
28-29	0.0026591230300884203
30-31	0.0021130600785596674
32-33	0.004899059772448472
34-35	0.009454223001060177
36-37	0.0013138135435818948
38-39	0.0
40-41	1.0084942722109404E-4
42-43	6.106878704897578E-4
44-45	1.870134144722201E-4
46-47	4.733240258896882E-5
48-49	0.0046571329597086125
50-51	0.003624757012862945
52-53	4.3078828185380726E-4
54-55	0.00290673647052589
56-57	0.040577773132578106
58-59	0.06256762668353187
60-61	0.030613004735362985
62-63	0.010734967484402815
64-65	9.176664451405965E-4
66-67	2.318806118401824E-4
68-69	0.0015992811337922344
70-71	0.011317213622170778
72-73	0.016914347273156233
74-75	0.010720887001189224
76-77	0.01311490181834322
78-79	0.0027986804221809417
80-81	0.014655286640501137
82-83	0.045766579807655514
84-85	0.025577961990540597
86-87	0.004184823065326152
88-89	5.981518781789529E-5
90-91	1.2115848838792816E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	53.0
20-21	210.0
22-23	513.0
24-25	708.0
26-27	2393.0
28-29	6329.0
30-31	17621.0
32-33	21584.0
34-35	29554.0
36-37	46448.0
38-39	54575.0
40-41	49758.0
42-43	53598.0
44-45	64290.0
46-47	65057.0
48-49	60685.0
50-51	53685.0
52-53	48912.0
54-55	52499.0
56-57	54867.0
58-59	54292.0
60-61	52171.0
62-63	49151.0
64-65	50193.0
66-67	53464.0
68-69	55093.0
70-71	54013.0
72-73	49138.0
74-75	49034.0
76-77	52150.0
78-79	53496.0
80-81	52845.0
82-83	48803.0
84-85	47290.0
86-87	49274.0
88-89	52086.0
90-91	52619.0
92-93	51031.0
94-95	55633.0
96-97	159531.0
98-99	1220811.0
100-101	2600622.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.68984091227304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.76299627836947	78.6822970809308
2	6.612533954029544	11.464790330036498
3	1.5057858939337925	3.9160901877919594
4	0.5501027925380163	1.9075329428207106
5	0.22334912705820648	0.9681050146285489
6	0.11220332146142764	0.5836132852391877
7	0.06559198890661759	0.39803113584039795
8	0.044029029819047716	0.3053495672427978
9	0.03017888931337165	0.23545828021367568
>10	0.08980744687492138	1.1968287943232698
>50	0.002421003010223686	0.1406612746009731
>100	9.365983728598803E-4	0.16442311001265503
>500	6.367631253559549E-5	0.03681899631856191
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.771140644684568E-5	0.0
2	0.0	5.3134219340537036E-5	0.0	1.771140644684568E-5	3.542281289369136E-5
3	0.0	5.3134219340537036E-5	0.0	3.542281289369136E-5	3.542281289369136E-5
4	0.0	1.4169125157476544E-4	0.0	3.542281289369136E-5	3.542281289369136E-5
5	0.0	2.1253687736214814E-4	0.0	3.542281289369136E-5	3.542281289369136E-5
6	1.594026580216111E-4	2.1253687736214814E-4	0.0	5.3134219340537036E-5	3.542281289369136E-5
7	1.594026580216111E-4	2.302482838089938E-4	0.0	7.084562578738272E-5	3.542281289369136E-5
8	1.594026580216111E-4	2.302482838089938E-4	0.0	7.084562578738272E-5	3.542281289369136E-5
9	1.594026580216111E-4	3.5422812893691355E-4	0.0	7.084562578738272E-5	3.542281289369136E-5
10-11	1.594026580216111E-4	3.5422812893691355E-4	0.0	9.741273545765122E-5	3.542281289369136E-5
12-13	1.594026580216111E-4	3.5422812893691355E-4	0.0	1.2397984512791974E-4	4.42785161171142E-5
14-15	1.9482547091530247E-4	5.224864901819475E-4	0.0	1.2397984512791974E-4	1.328355483513426E-4
16-17	2.302482838089938E-4	5.49053599852216E-4	0.0	1.594026580216111E-4	2.1253687736214814E-4
18-19	2.302482838089938E-4	5.933321159693303E-4	0.0	2.833825031495309E-4	2.656710967026852E-4
20-21	2.302482838089938E-4	6.376106320864444E-4	0.0	3.365167224900679E-4	2.656710967026852E-4
22-23	2.302482838089938E-4	6.553220385332901E-4	0.0	3.365167224900679E-4	3.2766101926664506E-4
24-25	2.302482838089938E-4	7.350233675440956E-4	0.0	3.365167224900679E-4	3.8965094183060495E-4
26-27	2.302482838089938E-4	7.615904772143642E-4	0.0	4.073623482774506E-4	4.3392945794771913E-4
28-29	2.302482838089938E-4	7.615904772143642E-4	0.0	7.70446180437787E-4	4.42785161171142E-4
30-31	2.302482838089938E-4	8.324361030017469E-4	0.0	0.002160791586515173	4.42785161171142E-4
32-33	2.3910398703241663E-4	8.324361030017469E-4	0.0	0.004985760914787058	4.95919380511679E-4
34-35	2.479596902558395E-4	9.564159481296666E-4	0.0	0.008660877752507536	4.95919380511679E-4
36-37	2.479596902558395E-4	0.001098107199704432	0.0	0.014204547970370233	5.136307869585247E-4
38-39	2.479596902558395E-4	0.0011158186061512778	0.0	0.02192672118119495	5.401978966287932E-4
40-41	2.479596902558395E-4	0.0012220870448323518	0.0	0.030295360727329534	5.667650062990617E-4
42-43	2.479596902558395E-4	0.0012840769673963118	0.0	0.03725594346093988	5.756207095224846E-4
44-45	2.5681539347926233E-4	0.0014966138447584597	0.0	0.04471244557506192	5.844764127459073E-4
46-47	2.656710967026852E-4	0.0016294493931098024	0.0	0.05311650793409019	6.198992256395987E-4
48-49	2.74526799926108E-4	0.0016294493931098024	0.0	0.062096191002640945	6.553220385332901E-4
50-51	3.010939095963765E-4	0.0017445735350142995	0.0	0.07039398492298815	6.553220385332901E-4
52-53	3.188053160432222E-4	0.001771140644684568	0.0	0.07854123188853716	6.730334449801358E-4
54-55	3.188053160432222E-4	0.0018331305672485278	0.0	0.08588260986075469	6.907448514269814E-4
56-57	3.188053160432222E-4	0.0018774090833656419	0.0	0.09333025627165331	6.907448514269814E-4
58-59	3.365167224900679E-4	0.001921687599482756	0.0	0.10167232870811761	6.907448514269814E-4
60-61	3.365167224900679E-4	0.001992533225270139	0.0	0.10961589449952791	6.996005546504043E-4
62-63	3.5422812893691355E-4	0.00202795603816383	0.0	0.11704582950397965	7.084562578738271E-4
64-65	3.5422812893691355E-4	0.00208994596072779	0.0	0.12405069075370714	7.084562578738271E-4
66-67	3.7193953538375925E-4	0.0021076573671746355	0.0	0.13116182044211566	7.084562578738271E-4
68-69	3.7193953538375925E-4	0.00215193588329175	0.0	0.13764419520166118	7.084562578738271E-4
70-71	3.896509418306049E-4	0.0021873586961854413	0.0	0.14360408347102477	7.438790707675185E-4
72-73	4.604965676179876E-4	0.0022316372123025556	0.0	0.14824447196009832	8.501475094485926E-4
74-75	4.604965676179876E-4	0.0022847714316430925	0.0	0.1519195887978188	8.501475094485926E-4
76-77	4.604965676179876E-4	0.0023821841671007437	0.0	0.15510764195825102	8.590032126720154E-4
78-79	4.604965676179876E-4	0.0024353183864412806	0.0	0.15718873221575538	8.678589158954383E-4
80-81	4.604965676179876E-4	0.0024441740896647035	0.0	0.1578883327704058	8.85570322342284E-4
82-83	4.604965676179876E-4	0.0024441740896647035	0.0	0.15822484949289586	9.209931352359752E-4
84-85	4.604965676179876E-4	0.0024707411993349724	0.0	0.15844624207348146	9.209931352359752E-4
86-87	4.6935227084141047E-4	0.002479596902558395	0.0	0.158499376292822	9.387045416828209E-4
88	4.782079740648333E-4	0.0026035767476863146	0.0	0.158499376292822	9.387045416828209E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10470	0.0	46.756874	1
GTATCAA	19575	0.0	37.046722	1
TCAACGC	22630	0.0	31.36691	4
TATCAAC	22875	0.0	31.321415	2
ATCAACG	22940	0.0	30.906864	3
CAACGCA	23025	0.0	30.828802	5
AACGCAG	23905	0.0	29.65921	6
ACGCAGA	27725	0.0	25.557749	7
GTGGTAT	5495	0.0	25.292238	1
CGCAGAG	28170	0.0	25.12456	8
TGGTATC	5245	0.0	24.836723	2
GCAGAGT	31905	0.0	22.26134	9
GTACATG	26120	0.0	21.902882	1
TACATGG	26185	0.0	21.452417	2
ACATGGG	26460	0.0	20.774584	3
GATCAAC	3625	0.0	20.485905	1
GAGTACT	17265	0.0	20.256634	12-13
CAGAGTA	31495	0.0	18.843107	10-11
CATGGGG	16530	0.0	18.672668	4
GTACTTT	19415	0.0	17.992542	14-15
>>END_MODULE