FastQCFastQC Report
Fri 27 May 2016
SRR2139576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5620664
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111200.043.9488141
GTATCAA192850.034.689311
CTTATAC47000.031.82911
TATCAAC217150.030.265592
TCAACGC218700.030.0308044
ATCAACG219550.029.8765263
CAACGCA224650.029.272835
AACGCAG232650.028.2481786
GCTCCGA12700.027.4629121
TATACAC68700.025.6893523
ACGCAGA269150.024.3705467
GATCAAC29850.023.9318791
CGCAGAG274800.023.8388968
TGGTATC57750.022.3387372
GTGGTAT62300.021.584111
TACATGG256800.021.3380532
GCAGAGT309300.021.315719
GTACATG263800.021.1860051
TTATACA78500.021.1446082
ACATGGG258500.020.6769853
Produced by FastQC (version 0.11.5)