##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5620664
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.28827110106564	25.0	18.0	33.0	18.0	33.0
2	27.638315330715375	29.0	25.0	31.0	18.0	33.0
3	26.95372699737967	29.0	25.0	31.0	18.0	33.0
4	30.94803140696544	31.0	30.0	33.0	27.0	33.0
5	31.699679966637394	33.0	31.0	33.0	29.0	33.0
6	36.12243393307268	38.0	36.0	38.0	33.0	38.0
7	36.28812449916949	38.0	36.0	38.0	33.0	38.0
8	36.512230227603006	38.0	37.0	38.0	34.0	38.0
9	37.18143301218504	38.0	38.0	38.0	36.0	38.0
10-11	37.22562458812695	38.0	38.0	38.0	36.0	38.0
12-13	37.342011192983605	38.0	38.0	38.0	36.5	38.0
14-15	37.33455815896485	38.0	38.0	38.0	36.0	38.0
16-17	37.45454869744927	38.0	38.0	38.0	37.0	38.0
18-19	37.473841969560894	38.0	38.0	38.0	37.0	38.0
20-21	37.5016009699206	38.0	38.0	38.0	37.0	38.0
22-23	37.53179959068248	38.0	38.0	38.0	37.5	38.0
24-25	37.54081398688709	38.0	38.0	38.0	38.0	38.0
26-27	37.50304820249604	38.0	38.0	38.0	37.0	38.0
28-29	37.4687618168977	38.0	38.0	38.0	37.0	38.0
30-31	37.49080223647877	38.0	38.0	38.0	37.0	38.0
32-33	37.47737118688371	38.0	38.0	38.0	37.0	38.0
34-35	37.438406224174955	38.0	38.0	38.0	37.0	38.0
36-37	37.41920242479253	38.0	38.0	38.0	37.0	38.0
38-39	37.40123593873904	38.0	38.0	38.0	37.0	38.0
40-41	37.41345566297163	38.0	38.0	38.0	37.0	38.0
42-43	37.38555592409283	38.0	38.0	38.0	37.0	38.0
44-45	37.347927678825556	38.0	38.0	38.0	37.0	38.0
46-47	37.30801427609665	38.0	38.0	38.0	37.0	38.0
48-49	37.24711908534728	38.0	38.0	38.0	37.0	38.0
50-51	37.17054284302857	38.0	38.0	38.0	37.0	38.0
52-53	37.19756341894579	38.0	38.0	38.0	37.0	38.0
54-55	37.185761116553564	38.0	38.0	38.0	37.0	38.0
56-57	37.146964934737404	38.0	38.0	38.0	37.0	38.0
58-59	37.136416871815555	38.0	38.0	38.0	37.0	38.0
60-61	37.15948287390067	38.0	38.0	38.0	37.0	38.0
62-63	37.163316383151944	38.0	38.0	38.0	37.0	38.0
64-65	37.114777130332726	38.0	38.0	38.0	36.0	38.0
66-67	37.157064952338985	38.0	38.0	38.0	36.0	38.0
68-69	37.175104557080836	38.0	38.0	38.0	36.0	38.0
70-71	37.16342612096382	38.0	38.0	38.0	36.0	38.0
72-73	37.16614719080711	38.0	38.0	38.0	36.0	38.0
74-75	37.13275265787936	38.0	38.0	38.0	36.0	38.0
76-77	37.14703418197325	38.0	38.0	38.0	36.0	38.0
78-79	37.154077969355455	38.0	38.0	38.0	36.0	38.0
80-81	37.156419181328175	38.0	38.0	38.0	36.0	38.0
82-83	37.11554171250063	38.0	38.0	38.0	36.0	38.0
84-85	37.10613111358683	38.0	38.0	38.0	36.0	38.0
86-87	37.084845613171055	38.0	38.0	38.0	36.0	38.0
88-89	37.098236849470425	38.0	38.0	38.0	36.0	38.0
90-91	37.0940440877855	38.0	38.0	38.0	36.0	38.0
92-93	37.083114084804045	38.0	38.0	38.0	36.0	38.0
94-95	37.07351221270474	38.0	38.0	38.0	36.0	38.0
96-97	37.06087493318893	38.0	38.0	38.0	36.0	38.0
98-99	37.0929507216464	38.0	38.0	38.0	36.0	38.0
100	35.72512018273461	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	28.0
21	75.0
22	279.0
23	824.0
24	2082.0
25	4075.0
26	7434.0
27	12927.0
28	21231.0
29	31554.0
30	41615.0
31	53160.0
32	68967.0
33	94814.0
34	155026.0
35	337470.0
36	1207838.0
37	3581257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.817032293693416	19.36112886306671	9.55449391744463	21.267344925795246
2	14.96261086458228	20.43966684002069	41.29716253923373	23.300559756163306
3	19.738154234119726	26.362492365189212	29.50731385072546	24.3920395499656
4	12.76630305600904	17.688906506419883	38.824256351206905	30.72053408636417
5	13.279623190427323	38.31193254035467	34.73555793408038	13.672886335137626
6	29.990460201855157	37.288085535801464	18.62607336072749	14.095380901615895
7	26.75074119567708	33.46676397454528	22.29550756425238	17.486987265525254
8	24.693078967182526	36.29393253181475	21.079466767627455	17.933521733375272
9	25.976183596813474	17.04725633839703	21.40159952631931	35.574960538470194
10-11	24.35607252096905	26.955044813210684	28.23186904607712	20.457013619743147
12-13	25.196373595717514	24.646064948909952	28.705060469723865	21.45250098564867
14-15	22.481827769815098	25.925673550313626	26.59815637440701	24.994342305464265
16-17	21.52833722136744	29.339175940778528	27.374737219659455	21.757749618194577
18-19	21.44252885424213	28.086966237440986	29.42268920540349	21.047815702913393
20-21	22.5515792814809	27.034186088488017	29.44764356989129	20.96659106013979
22-23	22.126629102977503	27.135950336574155	29.34760261252537	21.389817947922975
24-25	21.886204537586657	27.271971826011043	29.602413327108906	21.239410309293394
26-27	21.726621969922462	27.41485439299232	29.603437636117956	21.255086000967257
28-29	21.337959738804912	27.283752018161362	29.789762208195725	21.588526034838
30-31	21.905572131107206	27.06089259791373	29.77704842973244	21.25648684124663
32-33	21.524957963831824	27.485147697229483	29.595509016125764	21.394385322812926
34-35	21.644641446392047	27.521505863278932	29.769342722709997	21.064509967619024
36-37	21.84306925144261	27.2150305425139	29.500970750618905	21.44092945542458
38-39	21.552295732138617	27.338410208000745	29.66569775751053	21.44359630235011
40-41	21.777478221863326	27.199490063975208	29.631553846217155	21.39147786794431
42-43	21.805791080444656	27.161132563788904	29.458294188390344	21.574782167376096
44-45	21.686948261643312	27.464495239614546	29.286589679065244	21.561966819676897
46-47	21.97256625032639	27.29181890419039	28.840311682261426	21.895303163221794
48-49	21.859860073955026	27.43039921841366	28.90506840256365	21.804672305067662
50-51	21.715243072343142	27.731443903718482	28.786732864271315	21.766580159667058
52-53	21.87382712470406	27.78240324684737	28.789525051939897	21.554244576508673
54-55	21.860227894831894	27.78029287564768	28.55535128304266	21.804127946477763
56-57	22.009667127976126	28.062953930822083	28.218548694784207	21.708830246417584
58-59	21.68080654925212	28.302730077504574	28.584769078952533	21.431694294290775
60-61	21.926649445041182	28.498655384354123	28.207290211555403	21.367404959049292
62-63	21.69186392224592	28.604820529128173	28.25062038262134	21.452695166004574
64-65	21.680150112591612	28.524536307812333	28.305937867289877	21.489375712306185
66-67	21.876069587373966	28.464629428395938	28.1195446189522	21.539756365277892
68-69	21.80480768031108	28.287617869967196	28.33588821577641	21.571686233945314
70-71	21.827270353135066	27.88351015026882	28.488192102281907	21.80102739431421
72-73	21.9896887107833	27.812702094200226	28.453981183796078	21.743628011220395
74-75	21.794204920389983	27.972287293807245	28.40035048448111	21.833157301321666
76-77	21.84574884601572	27.804228475159277	28.466955359506052	21.883067319318954
78-79	21.895836761596524	27.856484408536602	28.33666332889852	21.911015500968354
80-81	21.84733229004261	27.919252847771407	28.29137310307246	21.942041759113526
82-83	21.997756632084254	27.788907488916692	28.459394927079178	21.753940951919876
84-85	22.081741856494506	27.77949977622952	28.31759398675844	21.821164380517533
86-87	21.81925931153092	27.94887439665727	28.354493876884483	21.877372414927326
88-89	22.00734066789653	27.839407862807654	28.27730274596485	21.875948723330968
90-91	22.059806190682135	27.898775265786806	28.272029294767908	21.769389248763147
92-93	22.087247617578242	27.910233082722137	28.240014824786012	21.76250447491361
94-95	21.848207843599287	27.94328497579851	28.28895095730003	21.919556223302173
96-97	22.05404744908131	28.048529515798137	28.322521567876663	21.574901467243887
98-99	23.024139980025875	29.931835567548216	28.563517366894985	18.480507085530927
100	22.044360312074055	30.789689431659006	22.494808100236103	24.671142156030836
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1059.0
1	1081.0
2	1425.0
3	1966.5
4	2580.0
5	3628.0
6	4705.0
7	5806.5
8	7611.5
9	8919.5
10	8638.5
11	8304.0
12	9103.5
13	11337.0
14	15356.5
15	19874.0
16	24247.0
17	28218.5
18	29195.5
19	27044.5
20	23976.5
21	21399.0
22	20883.0
23	23330.5
24	28280.0
25	35910.0
26	46522.0
27	59032.5
28	70769.0
29	83222.5
30	96321.0
31	108864.5
32	123793.5
33	138683.0
34	153165.0
35	167389.0
36	183559.5
37	196531.5
38	202464.0
39	208676.0
40	216091.5
41	220489.0
42	221542.5
43	230554.5
44	242823.5
45	248995.5
46	247429.0
47	242136.5
48	238206.5
49	231196.5
50	223484.0
51	216435.0
52	206638.5
53	194104.5
54	180560.0
55	167172.0
56	153676.5
57	138552.5
58	123767.0
59	110123.5
60	95651.0
61	79375.5
62	62959.0
63	49659.0
64	39149.0
65	29468.0
66	21396.0
67	15996.5
68	12158.5
69	8956.5
70	6597.5
71	4784.5
72	3252.0
73	2178.5
74	1458.0
75	971.5
76	654.0
77	424.5
78	290.0
79	198.5
80	141.5
81	95.0
82	60.0
83	41.5
84	26.5
85	17.5
86	13.0
87	12.0
88	8.5
89	6.5
90	8.5
91	5.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003220260097383512
3	4.803702907699161E-4
4	0.0
5	0.0
6	0.0
7	2.8466387601180216E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.693682210152209E-4
24-25	3.914719264360191E-4
26-27	8.008568100057982E-5
28-29	0.0027777604662364104
30-31	0.002184073929030434
32-33	0.004924160334592673
34-35	0.009656865650403352
36-37	0.0013154606085320777
38-39	0.0
40-41	1.0059703425310727E-4
42-43	6.634171511761832E-4
44-45	1.580393599814964E-4
46-47	2.817763141272406E-5
48-49	0.004440634270594984
50-51	0.0033910641245436315
52-53	3.8652795466800145E-4
54-55	0.00284585106589305
56-57	0.04229805115172183
58-59	0.06412277883253038
60-61	0.03073293998247129
62-63	0.011305139296045706
64-65	7.987698943626815E-4
66-67	1.656967709738196E-4
68-69	0.001321021552256939
70-71	0.010682960836754058
72-73	0.017017261333178723
74-75	0.011388796336806698
76-77	0.013605443670882672
78-79	0.0030423175264581693
80-81	0.01480353474264931
82-83	0.04572151677298102
84-85	0.025270431505534464
86-87	0.003574634717014855
88-89	7.043880558326148E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	76.0
20-21	225.0
22-23	467.0
24-25	632.0
26-27	2196.0
28-29	5216.0
30-31	13089.0
32-33	17269.0
34-35	23579.0
36-37	36748.0
38-39	43622.0
40-41	40528.0
42-43	45508.0
44-45	54106.0
46-47	54590.0
48-49	51465.0
50-51	46507.0
52-53	43181.0
54-55	46406.0
56-57	48889.0
58-59	49238.0
60-61	53757.0
62-63	48364.0
64-65	52993.0
66-67	57896.0
68-69	58849.0
70-71	64674.0
72-73	48161.0
74-75	47091.0
76-77	49344.0
78-79	51352.0
80-81	51003.0
82-83	47159.0
84-85	46529.0
86-87	48559.0
88-89	50205.0
90-91	50967.0
92-93	49529.0
94-95	54436.0
96-97	160059.0
98-99	353069.0
100-101	3553131.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.56761780335177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.41411418754467	80.04916213006868
2	6.239463258741834	10.927498678791213
3	1.380975382999757	3.627861736030801
4	0.4610590817347603	1.614953818164553
5	0.2037386477926773	0.8920454020840433
6	0.09872817670171773	0.5187234746302678
7	0.054430476683806496	0.33364430253712724
8	0.03739927017590324	0.26199719975102304
9	0.023481829131291838	0.18506230548233177
>10	0.0833629690196382	1.1326408442200089
>50	0.0019124301406937465	0.1126558387767485
>100	0.0012425011032125434	0.2521247555832965
>500	6.119215343160926E-5	0.048895866326123756
>1k	3.059607671383767E-5	0.04273364755375192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7791492250737635E-5	0.0	0.0	1.7791492250737635E-5
3	0.0	1.7791492250737635E-5	0.0	0.0	1.7791492250737635E-5
4	0.0	8.895746125368818E-5	0.0	3.558298450147527E-5	3.558298450147527E-5
5	1.7791492250737635E-5	1.0674895350442581E-4	0.0	3.558298450147527E-5	3.558298450147527E-5
6	1.7791492250737635E-5	1.0674895350442581E-4	0.0	3.558298450147527E-5	5.3374476752212904E-5
7	1.7791492250737635E-5	1.0674895350442581E-4	0.0	3.558298450147527E-5	5.3374476752212904E-5
8	1.7791492250737635E-5	1.0674895350442581E-4	0.0	5.3374476752212904E-5	5.3374476752212904E-5
9	1.7791492250737635E-5	2.1349790700885162E-4	0.0	8.895746125368818E-5	7.116596900295054E-5
10-11	1.7791492250737635E-5	2.1349790700885162E-4	0.0	1.0674895350442581E-4	7.116596900295054E-5
12-13	3.558298450147527E-5	2.1349790700885162E-4	0.0	1.4233193800590108E-4	8.006171512831936E-5
14-15	3.558298450147527E-5	2.8466387601180216E-4	0.0	1.95706414758114E-4	1.1564469962979462E-4
16-17	3.558298450147527E-5	2.8466387601180216E-4	0.0	2.2239365313422044E-4	1.3343619188053226E-4
18-19	3.558298450147527E-5	3.2024686051327744E-4	0.0	3.7362133726549036E-4	1.6012343025663872E-4
20-21	3.558298450147527E-5	3.558298450147527E-4	0.0	4.447873062684409E-4	1.8681066863274518E-4
22-23	3.558298450147527E-5	4.1810006789233447E-4	0.0	4.447873062684409E-4	2.046021608834828E-4
24-25	6.227022287758172E-5	5.070575291460226E-4	0.0	5.070575291460226E-4	2.3128939925958926E-4
26-27	7.116596900295054E-5	5.159532752713914E-4	0.0	6.227022287758172E-4	2.3128939925958926E-4
28-29	7.116596900295054E-5	5.159532752713914E-4	0.0	0.0012454044575516345	2.4018514538495805E-4
30-31	8.895746125368818E-5	5.693277520236043E-4	0.0	0.0026598280914852763	2.4908089151032687E-4
32-33	8.895746125368818E-5	5.782234981489731E-4	0.0	0.004759224177072318	2.4908089151032687E-4
34-35	9.7853207379057E-5	6.938681977787678E-4	0.0	0.008175190689213943	2.4908089151032687E-4
36-37	1.0674895350442581E-4	8.095128974085624E-4	0.0	0.01294331061241163	2.4908089151032687E-4
38-39	1.0674895350442581E-4	8.717831202861441E-4	0.0	0.01991757557470078	2.579766376356957E-4
40-41	1.0674895350442581E-4	9.607405815398323E-4	0.0	0.02714092142850026	2.668723837610645E-4
42-43	1.0674895350442581E-4	0.0010408022966681516	0.0	0.03410629064466405	2.668723837610645E-4
44-45	1.0674895350442581E-4	0.0011920299807994215	0.0	0.04184558977373491	2.93559622137171E-4
46-47	1.0674895350442581E-4	0.0012987789343038474	0.0	0.049540410172178945	3.2024686051327744E-4
48-49	1.0674895350442581E-4	0.0013254661726799539	0.0	0.05793799451452711	3.380383527640151E-4
50-51	1.0674895350442581E-4	0.0014411108723097485	0.0	0.06582852132772925	3.380383527640151E-4
52-53	1.0674895350442581E-4	0.0014589023645604862	0.0	0.07318530337340926	3.469340988893839E-4
54-55	1.0674895350442581E-4	0.001467798110685855	0.0	0.08037306624270726	3.6472559114012154E-4
56-57	1.0674895350442581E-4	0.0014766938568112238	0.0	0.08774763978063801	4.0030857564159677E-4
58-59	1.0674895350442581E-4	0.0014766938568112238	0.0	0.09463294728167349	4.092043217669656E-4
60-61	1.0674895350442581E-4	0.0014944853490619615	0.0	0.10174064843584317	4.2699581401770323E-4
62-63	1.0674895350442581E-4	0.001512276841312699	0.0	0.10811000266160724	4.2699581401770323E-4
64-65	1.0674895350442581E-4	0.0016724002715693376	0.0	0.11462168882537721	4.2699581401770323E-4
66-67	1.0674895350442581E-4	0.0016901917638200753	0.0	0.12117785371977403	4.2699581401770323E-4
68-69	1.0674895350442581E-4	0.001707983256070813	0.0	0.12750272921491126	4.2699581401770323E-4
70-71	1.0674895350442581E-4	0.0017168790021961818	0.0	0.13297361308201308	4.2699581401770323E-4
72-73	1.0674895350442581E-4	0.0017257747483215506	0.0	0.13720798823768865	4.625787985191785E-4
74-75	1.0674895350442581E-4	0.0017702534789483947	0.0	0.1407573909417108	4.892660368952849E-4
76-77	1.0674895350442581E-4	0.0018236279557006076	0.0	0.14415556596160167	4.981617830206537E-4
78-79	1.0674895350442581E-4	0.001850315194076714	0.0	0.14659300039995274	4.981617830206537E-4
80-81	1.0674895350442581E-4	0.001850315194076714	0.0	0.14736693031285983	5.159532752713914E-4
82-83	1.0674895350442581E-4	0.0018681066863274517	0.0	0.14790957082650733	5.33744767522129E-4
84-85	1.0674895350442581E-4	0.0019125854169542958	0.0	0.14809638149514007	5.33744767522129E-4
86-87	1.0674895350442581E-4	0.0019214811630796646	0.0	0.14825650492539671	5.515362597728667E-4
88	1.0674895350442581E-4	0.0019748556398318773	0.0	0.14825650492539671	5.515362597728667E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11120	0.0	43.948814	1
GTATCAA	19285	0.0	34.68931	1
CTTATAC	4700	0.0	31.8291	1
TATCAAC	21715	0.0	30.26559	2
TCAACGC	21870	0.0	30.030804	4
ATCAACG	21955	0.0	29.876526	3
CAACGCA	22465	0.0	29.27283	5
AACGCAG	23265	0.0	28.248178	6
GCTCCGA	1270	0.0	27.462912	1
TATACAC	6870	0.0	25.689352	3
ACGCAGA	26915	0.0	24.370546	7
GATCAAC	2985	0.0	23.931879	1
CGCAGAG	27480	0.0	23.838896	8
TGGTATC	5775	0.0	22.338737	2
GTGGTAT	6230	0.0	21.58411	1
TACATGG	25680	0.0	21.338053	2
GCAGAGT	30930	0.0	21.31571	9
GTACATG	26380	0.0	21.186005	1
TTATACA	7850	0.0	21.144608	2
ACATGGG	25850	0.0	20.676985	3
>>END_MODULE