FastQCFastQC Report
Fri 27 May 2016
SRR2139575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876516
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90700.052.4853481
GTATCAA163800.038.4795571
TATCAAC194750.031.9022872
TCAACGC194450.031.8405154
ATCAACG197300.031.3805793
CAACGCA201400.030.7842985
AACGCAG208050.029.7385436
TGGTATC39650.027.4487022
GTGGTAT42400.026.7800451
ACGCAGA244400.025.3505447
CGCAGAG249600.024.8393638
GCAGAGT285850.021.7493239
GTACATG234050.021.3828451
GAGTACT160850.020.9371712-13
TACATGG231050.020.8259622
GATCAAC29750.020.4518531
ACATGGG230400.020.326283
GTACTTT174150.019.22754514-15
CAGAGTA285450.019.07796310-11
CATGGGG149350.018.1314324
Produced by FastQC (version 0.11.5)