##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139575_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5876516
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.296827405898323	25.0	18.0	33.0	18.0	33.0
2	27.325844599078774	28.0	25.0	31.0	18.0	33.0
3	26.780944865971605	29.0	25.0	31.0	18.0	33.0
4	30.97584419067352	31.0	30.0	33.0	28.0	33.0
5	31.759987210108847	33.0	31.0	33.0	29.0	33.0
6	36.05621494096162	38.0	36.0	38.0	33.0	38.0
7	36.45585802880482	38.0	37.0	38.0	34.0	38.0
8	36.515437208032786	38.0	37.0	38.0	34.0	38.0
9	37.18131865887883	38.0	38.0	38.0	36.0	38.0
10-11	37.22851311899772	38.0	38.0	38.0	36.0	38.0
12-13	37.325300143826716	38.0	38.0	38.0	36.5	38.0
14-15	37.30223715888802	38.0	38.0	38.0	36.0	38.0
16-17	37.405657706028535	38.0	38.0	38.0	37.0	38.0
18-19	37.43464996947171	38.0	38.0	38.0	37.0	38.0
20-21	37.46801583377449	38.0	38.0	38.0	37.0	38.0
22-23	37.488644432526655	38.0	38.0	38.0	37.0	38.0
24-25	37.49770919027658	38.0	38.0	38.0	37.0	38.0
26-27	37.472718341724146	38.0	38.0	38.0	37.0	38.0
28-29	37.448813352004365	38.0	38.0	38.0	37.0	38.0
30-31	37.45870345126842	38.0	38.0	38.0	37.0	38.0
32-33	37.44673453968039	38.0	38.0	38.0	37.0	38.0
34-35	37.41331822918403	38.0	38.0	38.0	37.0	38.0
36-37	37.39469756187705	38.0	38.0	38.0	37.0	38.0
38-39	37.38225414087319	38.0	38.0	38.0	37.0	38.0
40-41	37.364981748290134	38.0	38.0	38.0	37.0	38.0
42-43	37.33406976964113	38.0	38.0	38.0	37.0	38.0
44-45	37.293676995887935	38.0	38.0	38.0	37.0	38.0
46-47	37.24406597604156	38.0	38.0	38.0	37.0	38.0
48-49	37.18109073263728	38.0	38.0	38.0	37.0	38.0
50-51	37.09908740890714	38.0	38.0	38.0	36.0	38.0
52-53	37.123978752742374	38.0	38.0	38.0	36.0	38.0
54-55	37.10664300086766	38.0	38.0	38.0	36.0	38.0
56-57	37.05630704581553	38.0	38.0	38.0	36.0	38.0
58-59	37.04378535856816	38.0	38.0	38.0	36.0	38.0
60-61	37.06254561604409	38.0	38.0	38.0	36.0	38.0
62-63	37.06541574350332	38.0	38.0	38.0	36.0	38.0
64-65	37.00876916719716	38.0	38.0	38.0	36.0	38.0
66-67	37.05344906433173	38.0	38.0	38.0	36.0	38.0
68-69	37.06671785453237	38.0	38.0	38.0	36.0	38.0
70-71	37.04841649334796	38.0	38.0	38.0	36.0	38.0
72-73	37.044047009091884	38.0	38.0	38.0	36.0	38.0
74-75	37.0062951182866	38.0	38.0	38.0	36.0	38.0
76-77	37.012832177369	38.0	38.0	38.0	36.0	38.0
78-79	37.0116033030918	38.0	38.0	38.0	36.0	38.0
80-81	37.00943400862896	38.0	38.0	38.0	36.0	38.0
82-83	36.958887497707394	38.0	38.0	38.0	36.0	38.0
84-85	36.945415158689045	38.0	38.0	38.0	36.0	38.0
86-87	36.91819558756041	38.0	38.0	38.0	35.0	38.0
88-89	36.924518741322785	38.0	38.0	38.0	35.0	38.0
90-91	36.91343523423542	38.0	38.0	38.0	35.0	38.0
92-93	36.89509222036695	38.0	38.0	38.0	35.0	38.0
94-95	36.88172016285284	38.0	38.0	38.0	35.0	38.0
96-97	36.86451294786899	38.0	38.0	38.0	35.0	38.0
98-99	36.90389056230621	38.0	38.0	38.0	35.0	38.0
100	35.41710110366185	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	15.0
21	87.0
22	348.0
23	993.0
24	2409.0
25	4840.0
26	9098.0
27	15477.0
28	25413.0
29	36425.0
30	48431.0
31	61437.0
32	79360.0
33	108462.0
34	171264.0
35	352365.0
36	1281646.0
37	3678440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.51544418495585	18.717774273055667	10.94493744252547	20.821844099463014
2	15.143357114012653	20.191789479810474	40.89752139895265	23.767332007224226
3	19.789619134567687	25.569894248007095	29.58642794119274	25.054058676232476
4	13.138116624283247	17.51052324916549	38.76632941445538	30.585030712095886
5	13.740692614467484	37.81936439890575	34.57912817730778	13.860814809318992
6	30.478637342261976	37.137038340404416	18.423841609552326	13.960482707781278
7	27.106112974370294	33.077897885153085	22.352008448564888	17.463980691911736
8	24.674620132064646	36.426243032436226	20.839694812368418	18.05944202313071
9	26.0030092660345	17.153224801906436	21.34991209076943	35.493853841289635
10-11	24.515044288146242	26.85687403897139	28.228009589355324	20.400072083527043
12-13	25.249824896248047	24.502239081796084	28.70521410985693	21.54272191209894
14-15	22.504737500927423	25.7299478126155	26.627494930669805	25.137819755787273
16-17	21.681834951185362	28.969146004197043	27.40055502273796	21.94846402187963
18-19	21.64440631149477	27.953365565583415	29.40535684749263	20.996871275429182
20-21	22.608245363076623	27.02381791084297	29.383690881077555	20.984245845002857
22-23	22.326470924091225	26.837092645673106	29.323870057771124	21.512566372464548
24-25	21.947864823102943	26.95480107814473	29.48222400623318	21.615110092519142
26-27	21.84932716420019	27.14975750847135	29.41477349372058	21.586141833607876
28-29	21.783564648171073	27.18162986430186	29.476125810591032	21.55867967693603
30-31	22.06789036902521	27.0486251462089	29.35637738458383	21.527107100182057
32-33	21.86482700363979	27.231684057455467	29.269790191414007	21.633698747490733
34-35	22.005285436807686	27.227548225133937	29.268121317080197	21.499045020978176
36-37	21.963178032442375	27.04619068318556	29.285255553134874	21.705375731237186
38-39	21.811103301177955	27.268676897294377	29.332120870065026	21.588098931462643
40-41	22.082652635357608	27.207295513971623	29.16799321088176	21.54205863978901
42-43	22.003248790298322	27.253100619711752	28.954672517131925	21.788978072858008
44-45	22.02567532881688	27.38138126777902	28.83523942592366	21.757703977480435
46-47	22.102508185846588	27.38007603200886	28.556515562874274	21.960900219270275
48-49	22.060983454505354	27.496857661632358	28.493074978186517	21.94908390567577
50-51	22.055152666493484	27.687237591992464	28.317080303199617	21.940529438314435
52-53	22.225693842049886	27.61749400391873	28.19734587355502	21.95946628047637
54-55	22.28018405581489	27.51160149227182	28.216750632492865	21.991463819420424
56-57	22.1685831710384	27.69118491490754	28.187333767728003	21.952898146326056
58-59	22.117410959874125	27.653108157546097	28.242551073469148	21.98692980911063
60-61	22.183065567356174	27.654642263001826	28.277587807203975	21.88470436243803
62-63	22.14192310209042	27.670523221431104	28.275204213981663	21.912349462496817
64-65	22.11396048202456	27.571833573771432	28.299961482055664	22.014244462148344
66-67	22.24622553298526	27.593691466803726	28.130996840986345	22.02908615922467
68-69	22.19964820873662	27.595604137211634	28.23881591183437	21.965931742217375
70-71	22.137591531994868	27.313651026895474	28.42587603566214	22.12288140544752
72-73	22.260563705307895	27.402741927209046	28.276156532029194	22.06053783545386
74-75	22.02880650134609	27.64224540751954	28.180162769335677	22.148785321798695
76-77	22.141280817971797	27.514726049097206	28.235784412635816	22.108208720295178
78-79	22.16531024959328	27.542881770882833	28.089152000878244	22.202655978645645
80-81	22.126157118557245	27.628298957731307	28.03970406662113	22.205839857090318
82-83	22.30799324404269	27.521139304217623	28.127101264555904	22.04376618718378
84-85	22.300103142232825	27.523551854835926	28.05581593657223	22.120529066359016
86-87	22.161427770793896	27.557654281368325	28.101652108750645	22.17926583908713
88-89	22.21575410729141	27.582942665695104	28.044283623694344	22.157019603319146
90-91	22.273813298366857	27.64866215613463	28.00906047301473	22.068464072483785
92-93	22.321486533694586	27.6359695507584	27.965497633542192	22.077046282004826
94-95	22.178300639486253	27.68745572833889	27.96506879561119	22.16917483656367
96-97	22.361262156780022	27.76266269798423	28.020363615315347	21.8557115299204
98-99	23.345891328980713	29.689089669563014	28.225286672474486	18.73973232898179
100	22.39128593519632	30.601726725394062	22.201450619741554	24.805536719668066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1214.0
1	1184.5
2	1477.0
3	1955.0
4	2451.5
5	3431.5
6	4482.5
7	5626.0
8	7666.5
9	9157.5
10	8866.5
11	8468.5
12	9207.5
13	11327.5
14	15557.0
15	20361.0
16	24564.5
17	28281.0
18	29174.0
19	26879.0
20	23743.5
21	21117.5
22	20140.0
23	22195.5
24	27022.0
25	34392.0
26	44863.5
27	56896.5
28	68330.0
29	80596.5
30	93092.5
31	105084.0
32	119355.5
33	134678.5
34	149397.0
35	163428.0
36	179062.5
37	192544.5
38	200200.0
39	207375.0
40	216041.0
41	223186.5
42	226508.0
43	230578.0
44	236136.0
45	241178.5
46	243498.0
47	242764.5
48	242299.5
49	241233.0
50	237711.0
51	230602.5
52	222385.0
53	211408.0
54	197183.0
55	183568.0
56	168463.5
57	151841.5
58	136807.0
59	121370.5
60	103938.5
61	85329.5
62	67305.5
63	52377.0
64	39976.0
65	29856.5
66	22174.5
67	16803.0
68	12993.5
69	9645.5
70	6992.0
71	5057.0
72	3572.5
73	2432.0
74	1624.0
75	1109.5
76	744.0
77	460.5
78	285.0
79	205.5
80	150.5
81	110.5
82	85.0
83	66.5
84	48.5
85	28.0
86	15.0
87	12.0
88	9.5
89	6.5
90	5.5
91	2.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00301198873618314
3	5.275234509699285E-4
4	6.806754206063593E-5
5	0.0
6	0.0
7	2.5525328272738473E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	3.7439537274548045E-4
24-25	4.935600623451455E-4
26-27	1.0212846791042686E-4
28-29	0.0027245657318937066
30-31	0.002096874225392499
32-33	0.004245642812959615
34-35	0.009287166292795395
36-37	0.0015229394693511012
38-39	0.0
40-41	1.2198092845609435E-4
42-43	5.875922443085771E-4
44-45	2.5632130317286E-4
46-47	2.6755497295955663E-5
48-49	0.004111932377877408
50-51	0.0034103714474995085
52-53	4.293827139289743E-4
54-55	0.002640972731910533
56-57	0.039870100818769946
58-59	0.06097062201346084
60-61	0.02911786165962577
62-63	0.010721087629175336
64-65	8.899719113381273E-4
66-67	2.3156333715573647E-4
68-69	0.0015085161087135977
70-71	0.01095774522594311
72-73	0.016408619585514508
74-75	0.010015540564087627
76-77	0.011845318273931647
78-79	0.002287043239347715
80-81	0.013595012877298801
82-83	0.04291601034375269
84-85	0.02303710541121233
86-87	0.0038689821382694094
88-89	7.449560092834289E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	60.0
20-21	202.0
22-23	495.0
24-25	551.0
26-27	1768.0
28-29	4681.0
30-31	12232.0
32-33	15714.0
34-35	21900.0
36-37	33288.0
38-39	37936.0
40-41	36600.0
42-43	42060.0
44-45	49694.0
46-47	50523.0
48-49	45850.0
50-51	40520.0
52-53	38550.0
54-55	41954.0
56-57	42749.0
58-59	42611.0
60-61	41267.0
62-63	40109.0
64-65	42051.0
66-67	44444.0
68-69	45175.0
70-71	44002.0
72-73	41744.0
74-75	42797.0
76-77	44486.0
78-79	45971.0
80-81	45648.0
82-83	42628.0
84-85	42492.0
86-87	44073.0
88-89	45887.0
90-91	46514.0
92-93	46313.0
94-95	54348.0
96-97	175294.0
98-99	397917.0
100-101	3943418.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.30388818336779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.83668372651962	81.09536250917903
2	5.970823338726152	10.544938329310337
3	1.2848825670901851	3.403803795992708
4	0.44397511648811344	1.5681891617025625
5	0.18298572077811395	0.8079175313371767
6	0.08744653601495199	0.46331214829723
7	0.05755677251771219	0.35577407632197156
8	0.032762119862050486	0.23144180551588794
9	0.02327916796638157	0.1850076940594676
>10	0.07739426920762758	1.0712410737112372
>50	0.0015493584482651583	0.09407765345457315
>100	5.495359146895219E-4	0.09121672013945287
>500	8.382788749870954E-5	0.04769565111584862
>1k	2.794257863391895E-5	0.040021849862546456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.4033771030317965E-5	0.0	0.0	0.0
3	0.0	3.4033771030317965E-5	0.0	0.0	0.0
4	0.0	5.105065654547695E-5	0.0	1.7016885515158983E-5	0.0
5	0.0	5.105065654547695E-5	0.0	1.7016885515158983E-5	0.0
6	5.105065654547695E-5	5.105065654547695E-5	0.0	1.7016885515158983E-5	0.0
7	5.105065654547695E-5	5.105065654547695E-5	0.0	1.7016885515158983E-5	0.0
8	5.105065654547695E-5	5.105065654547695E-5	0.0	1.7016885515158983E-5	1.7016885515158983E-5
9	5.105065654547695E-5	1.021013130909539E-4	0.0	1.7016885515158983E-5	1.7016885515158983E-5
10-11	5.105065654547695E-5	1.021013130909539E-4	0.0	3.4033771030317965E-5	1.7016885515158983E-5
12-13	5.105065654547695E-5	1.021013130909539E-4	0.0	5.105065654547695E-5	1.7016885515158983E-5
14-15	5.9559099303056436E-5	1.786772979091693E-4	0.0	1.021013130909539E-4	3.4033771030317965E-5
16-17	6.806754206063593E-5	1.871857406667488E-4	0.0	1.1911819860611287E-4	3.4033771030317965E-5
18-19	8.508442757579491E-5	1.956941834243283E-4	0.0	1.2762664136369237E-4	4.254221378789746E-5
20-21	8.508442757579491E-5	2.042026261819078E-4	0.0	1.3613508412127186E-4	5.105065654547695E-5
22-23	8.508442757579491E-5	2.3823639721222574E-4	0.0	1.7016885515158981E-4	5.105065654547695E-5
24-25	1.0210131309095388E-4	2.807786110001232E-4	0.0	2.042026261819078E-4	5.105065654547695E-5
26-27	1.1911819860611287E-4	3.0630393927286165E-4	0.0	3.2332082478802064E-4	6.806754206063593E-5
28-29	1.1911819860611287E-4	3.0630393927286165E-4	0.0	7.997936192124721E-4	7.657598481821543E-5
30-31	1.1911819860611287E-4	3.4033771030317963E-4	0.0	0.001820806750122011	8.508442757579491E-5
32-33	1.1911819860611287E-4	3.743714813334976E-4	0.0	0.003896866782971407	1.021013130909539E-4
34-35	1.1911819860611287E-4	4.1691369512139505E-4	0.0	0.007079024374306136	1.021013130909539E-4
36-37	1.1911819860611287E-4	4.594559089092925E-4	0.0	0.011724634119944538	1.021013130909539E-4
38-39	1.1911819860611287E-4	4.934896799396104E-4	0.0	0.01821657594397769	1.021013130909539E-4
40-41	1.3613508412127186E-4	5.615572220002464E-4	0.0	0.02511692302037466	1.021013130909539E-4
42-43	1.5315196963643082E-4	5.955909930305644E-4	0.0	0.031523780416832015	1.1060975584853338E-4
44-45	1.6166041239401032E-4	6.466416495760413E-4	0.0	0.03880700741732006	1.1911819860611287E-4
46-47	1.871857406667488E-4	6.636585350912003E-4	0.0	0.04651565655568708	1.1911819860611287E-4
48-49	1.956941834243283E-4	6.806754206063594E-4	0.0	0.05435193233541778	1.1911819860611287E-4
50-51	2.042026261819078E-4	7.827767336973132E-4	0.0	0.06226478409996672	1.1911819860611287E-4
52-53	2.042026261819078E-4	7.827767336973132E-4	0.0	0.06967563774181845	1.1911819860611287E-4
54-55	2.042026261819078E-4	7.827767336973132E-4	0.0	0.0764994088333972	1.1911819860611287E-4
56-57	2.2972795445464628E-4	7.827767336973132E-4	0.0	0.08205542195409661	1.1911819860611287E-4
58-59	2.5525328272738473E-4	7.997936192124721E-4	0.0	0.08706689473831093	1.1911819860611287E-4
60-61	2.5525328272738473E-4	8.083020619700516E-4	0.0	0.09224853637767684	1.1911819860611287E-4
62-63	2.5525328272738473E-4	8.168105047276312E-4	0.0	0.0973706189177397	1.3613508412127186E-4
64-65	2.5525328272738473E-4	8.84878046788267E-4	0.0	0.10241612547298434	1.3613508412127186E-4
66-67	2.6376172548496426E-4	9.018949323034261E-4	0.0	0.10766583465441087	1.3613508412127186E-4
68-69	2.892870537577027E-4	9.35928703333744E-4	0.0	0.11289852695032226	1.3613508412127186E-4
70-71	2.892870537577027E-4	9.444371460913235E-4	0.0	0.11783342374971836	1.5315196963643082E-4
72-73	2.892870537577027E-4	9.52945588848903E-4	0.0	0.1213048683948108	1.5315196963643082E-4
74-75	2.892870537577027E-4	0.0010125046881519595	0.0	0.12400204474896351	1.5315196963643082E-4
76-77	2.892870537577027E-4	0.0010635553446974363	0.0	0.12697149127135876	1.5315196963643082E-4
78-79	2.977954965152822E-4	0.001089080672970175	0.0	0.12899650064766266	1.5315196963643082E-4
80-81	3.0630393927286165E-4	0.001089080672970175	0.0	0.12962612541172353	1.5315196963643082E-4
82-83	3.2332082478802064E-4	0.001089080672970175	0.0	0.1301366319771783	1.7016885515158981E-4
84-85	3.4033771030317963E-4	0.0011060975584853338	0.0	0.13030680083232993	1.7016885515158981E-4
86-87	3.573545958183386E-4	0.0011401313295156517	0.0	0.13048547813023909	1.7016885515158981E-4
88	3.573545958183386E-4	0.0011401313295156517	0.0	0.13048547813023909	1.7016885515158981E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9070	0.0	52.485348	1
GTATCAA	16380	0.0	38.479557	1
TATCAAC	19475	0.0	31.902287	2
TCAACGC	19445	0.0	31.840515	4
ATCAACG	19730	0.0	31.380579	3
CAACGCA	20140	0.0	30.784298	5
AACGCAG	20805	0.0	29.738543	6
TGGTATC	3965	0.0	27.448702	2
GTGGTAT	4240	0.0	26.780045	1
ACGCAGA	24440	0.0	25.350544	7
CGCAGAG	24960	0.0	24.839363	8
GCAGAGT	28585	0.0	21.749323	9
GTACATG	23405	0.0	21.382845	1
GAGTACT	16085	0.0	20.93717	12-13
TACATGG	23105	0.0	20.825962	2
GATCAAC	2975	0.0	20.451853	1
ACATGGG	23040	0.0	20.32628	3
GTACTTT	17415	0.0	19.227545	14-15
CAGAGTA	28545	0.0	19.077963	10-11
CATGGGG	14935	0.0	18.131432	4
>>END_MODULE