FastQCFastQC Report
Fri 27 May 2016
SRR2139574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15056171
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151550.043.3142971
GTATCAA254850.032.030221
TCAACGC309050.025.66214
ATCAACG313250.025.3709133
CAACGCA316950.025.0615885
AACGCAG329700.024.0672956
TATCAAC335450.023.9267622
GTACATG383800.020.3945791
ACGCAGA389500.020.3828727
TACATGG381150.020.0039522
CGCAGAG401600.019.7790588
ACATGGG389550.018.945093
CATGGGG288750.017.2685834
AGAGTAC423700.016.83838810-11
GCAGAGT479550.016.4690119
TATACCG11100.015.6702915
GAGTACT273600.015.60782312-13
CAGAGTA462000.015.44247910-11
GTGGTAT86850.014.9256221
TATACTG81450.014.847135
Produced by FastQC (version 0.11.5)