##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15056171
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13883549808248	33.0	31.0	34.0	30.0	34.0
2	32.27206379364315	34.0	31.0	34.0	30.0	34.0
3	32.323551784846224	34.0	31.0	34.0	30.0	34.0
4	35.955705006272844	37.0	35.0	37.0	35.0	37.0
5	35.81787102444572	37.0	35.0	37.0	35.0	37.0
6	35.83964495355426	37.0	35.0	37.0	35.0	37.0
7	35.75561568741482	37.0	35.0	37.0	33.0	37.0
8	35.72219948883418	37.0	35.0	37.0	33.0	37.0
9	37.35595238656628	39.0	37.0	39.0	34.0	39.0
10-11	37.24840495634647	39.0	37.0	39.0	33.0	39.0
12-13	37.207391739905184	39.0	37.0	39.0	33.0	39.0
14-15	38.39877994212473	40.0	38.0	41.0	33.0	41.0
16-17	38.412786790213794	40.0	38.0	41.0	33.0	41.0
18-19	38.40780810074487	40.0	38.0	41.0	33.5	41.0
20-21	38.37558867460106	40.0	38.0	41.0	34.0	41.0
22-23	38.27181923446493	40.0	38.0	41.0	33.5	41.0
24-25	38.17716597991207	40.0	38.0	41.0	33.0	41.0
26-27	38.004038025466365	40.0	37.0	41.0	33.0	41.0
28-29	37.866183961188796	40.0	37.0	41.0	33.0	41.0
30-31	37.70972415332637	40.0	37.0	41.0	32.0	41.0
32-33	37.90445977034011	40.0	37.0	41.0	33.0	41.0
34-35	37.88508811998584	40.0	37.0	41.0	33.0	41.0
36-37	37.79578002574759	40.0	37.0	41.0	33.0	41.0
38-39	37.67937961595675	40.0	37.0	41.0	33.0	41.0
40-41	37.625496174668456	40.0	37.0	41.0	32.5	41.0
42-43	37.51153156808707	39.5	36.5	41.0	32.0	41.0
44-45	37.55493254583004	39.5	36.5	41.0	32.5	41.0
46-47	37.695983985741485	40.0	37.0	41.0	32.5	41.0
48-49	37.61964648261203	40.0	37.0	41.0	32.0	41.0
50-51	37.490787821312225	40.0	36.0	41.0	32.0	41.0
52-53	37.33763485966836	39.0	36.0	41.0	31.0	41.0
54-55	37.221890752885606	39.0	36.0	41.0	31.0	41.0
56-57	37.04055636393282	39.0	35.0	40.5	31.0	41.0
58-59	36.884594672241036	39.0	35.0	40.0	31.0	41.0
60-61	36.64636088799262	38.0	35.0	40.0	31.0	41.0
62-63	36.374147037362576	38.0	35.0	40.0	30.0	41.0
64-65	36.145348917812605	37.5	34.5	40.0	30.0	41.0
66-67	35.84643224697683	37.0	34.0	40.0	29.5	41.0
68-69	35.518925999807095	36.5	34.0	39.0	29.0	41.0
70-71	35.211434072374715	36.0	34.0	39.0	29.0	40.5
72-73	34.9010433229265	36.0	34.0	39.0	29.0	40.0
74-75	34.50968135693131	35.0	33.0	38.0	29.0	40.0
76-77	33.45142529205782	34.5	31.5	36.5	27.5	39.0
78-79	33.79466455469287	35.0	33.0	37.0	28.0	39.0
80-81	33.67403648028164	35.0	33.0	36.0	28.5	38.5
82-83	33.44827952187653	35.0	33.0	36.0	28.0	37.5
84-85	33.22817752858159	35.0	32.0	35.5	28.0	37.0
86-87	33.017383558462505	34.5	32.0	35.0	28.0	36.5
88-89	32.786601661083694	34.0	32.0	35.0	27.0	36.0
90-91	32.6428760292696	34.0	32.0	35.0	27.0	36.0
92-93	32.54999323607671	34.0	32.0	35.0	27.0	35.5
94-95	32.49970025472394	34.0	32.0	35.0	27.0	35.0
96-97	32.437429754104585	34.0	32.0	35.0	27.0	35.0
98-99	32.57321956162434	34.0	32.0	35.0	28.0	35.0
100-101	31.635366037450673	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	12.0
20	96.0
21	649.0
22	3016.0
23	10434.0
24	26808.0
25	56374.0
26	99713.0
27	156186.0
28	220797.0
29	289726.0
30	365712.0
31	452582.0
32	561092.0
33	710603.0
34	943196.0
35	1300271.0
36	1977696.0
37	3090997.0
38	3220007.0
39	1515451.0
40	54751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.7990877627519	21.668935614506506	14.49386434306571	25.03811227967589
2	14.597689888771809	22.209566494653124	40.18670316156665	23.00604045500842
3	17.5796422609706	26.482682748488973	31.16473637287993	24.772938617660493
4	12.548413014847709	18.47469804153485	38.124917516968345	30.8519714266491
5	12.927835370626436	37.9799286286002	35.9964562039047	13.095779796868673
6	30.501745762584658	38.20266786289821	18.128227953840323	13.16735842067681
7	26.18192899110936	32.54442978895498	23.518104304208553	17.755536915727113
8	24.29953804323822	37.24793641092413	21.10652170462198	17.34600384121567
9	25.384329123254513	16.087549749534595	21.19487750238756	37.33324362482334
10-11	24.17635267293391	26.65596717784356	29.246546150412346	19.921133998810188
12-13	24.681288489616648	24.322634885058093	29.159588450476555	21.836488174848707
14-15	21.875963682931072	25.81549452380688	26.46046262359799	25.84807916966405
16-17	21.777098948854277	28.7585465326777	27.30828863311071	22.15606588535732
18-19	21.795126397010236	28.101819513075405	28.72174472513629	21.38130936477807
20-21	22.42561114983541	27.361239140424143	28.565953462025544	21.647196247714902
22-23	22.192851695669617	27.41285828543794	28.372403617995385	22.02188640089706
24-25	22.1030539338398	27.4704575340892	28.48783857426586	21.93864995780514
26-27	22.061122736001995	27.618992411908515	28.42142343042202	21.898461421667463
28-29	22.04458731127471	27.64068068321091	28.384755968782894	21.92997603673148
30-31	22.147703398273514	27.59931148323343	28.37491692855714	21.878068189935913
32-33	22.041415184232033	27.730406356082998	28.27350190723824	21.954676552446724
34-35	22.202132783398767	27.640628258919335	28.354577124721175	21.802661832960727
36-37	22.104565656086525	27.648958024623816	28.259353492067056	21.987122827222603
38-39	21.99181634142131	27.640399480128604	28.360546369857882	22.007237808592212
40-41	22.169495991559458	27.51634760235906	28.29421865723438	22.019937748847102
42-43	22.112451064763153	27.63314134856229	28.10558230972216	22.148825276952397
44-45	22.111290108431376	27.776364559671517	27.93434545412994	22.177999877767167
46-47	22.230975862869457	27.64154636340448	27.716010531049367	22.411467242676697
48-49	22.16764657533935	27.740189252772357	27.70565568418131	22.386508487706987
50-51	22.116538106419448	27.86276294018586	27.632576643859093	22.3881223095356
52-53	22.23489115109402	27.81159482560915	27.594315806229204	22.35919821706763
54-55	22.219278802007473	27.701724778348375	27.58129715650231	22.49769926314184
56-57	22.277689326986984	27.822261012431877	27.477823541572953	22.42222611900819
58-59	22.156046375560084	27.872901189229538	27.592003290920463	22.379049144289915
60-61	22.279919156500945	27.86867149186633	27.48378455593279	22.36762479569994
62-63	22.20713062976956	27.98696933276589	27.449302426052007	22.356597611412543
64-65	22.18070152879976	27.905146149988603	27.433676158345598	22.480476162866044
66-67	22.247627781946193	27.9438175543506	27.268616604077405	22.5399380596258
68-69	22.232840386934036	27.91371252285077	27.31398306301753	22.539464027197663
70-71	22.23915231253912	27.695923942974087	27.37307612599802	22.691847618488772
72-73	22.294276773276238	27.705102535339332	27.339428988589553	22.661191702794877
74-75	22.230088768673493	27.824875579565273	27.286641995264226	22.65839365649701
76-77	22.35410104935356	27.820286102525564	27.213987102897754	22.611625745223122
78-79	22.33091206066161	27.770410149037794	27.212252447316104	22.686425342984492
80-81	22.286637444947015	27.821829393879526	27.223773448331933	22.66775971284153
82-83	22.35959602641411	27.770338870103423	27.300585187155065	22.569479916327403
84-85	22.431039819992556	27.797400495222984	27.228833412471126	22.54272627231333
86-87	22.302515294747856	27.843393520326664	27.267076354216552	22.58701483070893
88-89	22.335455579099577	27.812599623217515	27.321689248515753	22.53025554916716
90-91	22.353413296814363	27.86255642909766	27.318317408916414	22.465712865171568
92-93	22.39420912525201	27.790507741392474	27.36885141615612	22.446431717199395
94-95	22.137044311266653	27.86175874477968	27.47117861841552	22.530018325538144
96-97	22.049941249559307	27.917196926388787	27.544854650423193	22.48800717362871
98-99	22.20384001670908	28.458681777384427	27.636989329313444	21.70048887659305
100-101	25.07868179533366	36.29300229267077	28.74079986733336	9.887516044662206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4928.0
1	4262.5
2	4910.5
3	6609.5
4	8557.0
5	12525.0
6	15826.0
7	18102.0
8	19242.0
9	18706.0
10	17429.5
11	16260.0
12	18030.5
13	24018.5
14	37175.5
15	54726.5
16	67914.5
17	74029.0
18	75474.5
19	74705.0
20	72523.5
21	70978.0
22	72979.5
23	80955.5
24	95226.5
25	115840.5
26	142071.5
27	170948.5
28	202343.5
29	235131.5
30	266111.0
31	296817.5
32	329262.0
33	362440.0
34	394373.5
35	426201.5
36	462357.0
37	494391.5
38	518552.5
39	541095.0
40	562924.5
41	581108.5
42	594550.0
43	608031.5
44	620019.0
45	632003.0
46	647000.5
47	658090.5
48	657705.5
49	650509.0
50	641322.5
51	624724.0
52	597810.5
53	565735.5
54	530562.5
55	492127.5
56	452427.5
57	409755.5
58	365532.0
59	318073.0
60	267460.5
61	219293.0
62	175715.0
63	136286.5
64	102469.0
65	77109.0
66	57880.5
67	43909.5
68	33772.5
69	25073.0
70	18174.5
71	13038.5
72	9242.5
73	6403.0
74	4409.0
75	3015.0
76	2056.5
77	1401.0
78	981.5
79	717.0
80	519.0
81	368.5
82	271.0
83	185.5
84	123.0
85	92.0
86	74.5
87	64.0
88	46.5
89	30.0
90	20.0
91	12.5
92	8.5
93	4.5
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012353738543484927
3	0.0
4	2.922389762974929E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.003347464637589464
18-19	0.0
20-21	0.002138757825396062
22-23	0.0
24-25	3.3555109350121254E-4
26-27	0.0034666285813921014
28-29	0.0
30-31	1.3654420472092595E-4
32-33	7.712563766375426E-4
34-35	8.610802194394254E-4
36-37	0.009977652685455576
38-39	0.011368858386466885
40-41	0.025608652354666542
42-43	0.002129277681824379
44-45	0.001314837900487711
46-47	0.0017407727089602458
48-49	0.0017063856363282678
50-51	5.7462143490945166E-5
52-53	1.5228798005114484E-4
54-55	0.0061171073528179494
56-57	0.003674999614180554
58-59	0.0038787446031256167
60-61	0.0016029593281849666
62-63	4.755919316902706E-4
64-65	7.067999867494421E-4
66-67	6.181714449285037E-4
68-69	1.9973135334050288E-5
70-71	0.0
72-73	1.1509495868460931E-4
74-75	2.5855696356039945E-4
76-77	0.0013840898407213114
78-79	0.001882829540305878
80-81	0.01022276850490459
82-83	0.00929036213387083
84-85	0.012050034491302777
86-87	0.005698681327900376
88-89	0.08787615607277817
90-91	3.397205908190845E-4
92-93	3.4253937257650335E-5
94-95	2.1580075026392934E-4
96-97	0.014743848985607615
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	423.0
20-21	1502.0
22-23	2961.0
24-25	5987.0
26-27	8644.0
28-29	15260.0
30-31	35159.0
32-33	47648.0
34-35	69057.0
36-37	104265.0
38-39	108063.0
40-41	114031.0
42-43	131850.0
44-45	153846.0
46-47	155465.0
48-49	145608.0
50-51	135409.0
52-53	126818.0
54-55	145441.0
56-57	154156.0
58-59	160129.0
60-61	159747.0
62-63	158125.0
64-65	170691.0
66-67	184541.0
68-69	178426.0
70-71	176233.0
72-73	173248.0
74-75	177151.0
76-77	177686.0
78-79	190061.0
80-81	193262.0
82-83	186507.0
84-85	188380.0
86-87	201494.0
88-89	212979.0
90-91	229019.0
92-93	245140.0
94-95	311688.0
96-97	407000.0
98-99	1273647.0
100-101	8039424.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.17985084763659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.3475627633208	75.21264507016193
2	7.285316066126188	12.265536396487859
3	1.8583696442710245	4.693118384235309
4	0.6894841215403344	2.3216268205231634
5	0.3193173355978196	1.3440042841844584
6	0.16430189268909554	0.8298545292331474
7	0.09762592810700188	0.5752695246836658
8	0.058554463802098916	0.39432848234592177
9	0.04080446975706462	0.30914227602599376
>10	0.1349901836863278	1.7565827660704008
>50	0.002905509836199295	0.1653867874031257
>100	7.26892474508507E-4	0.09729591887477912
>500	1.358125549878208E-5	0.007419809723750166
>1k	2.7147536024449924E-5	0.027788950046403624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.6567179663408447E-5	0.0	0.0	0.0
5	0.0	3.985076949511267E-5	0.0	0.0	0.0
6	0.0	3.985076949511267E-5	0.0	0.0	6.641794915852112E-6
7	0.0	3.985076949511267E-5	0.0	0.0	1.3283589831704223E-5
8	0.0	3.985076949511267E-5	0.0	0.0	1.3283589831704223E-5
9	0.0	8.634333390607745E-5	0.0	6.641794915852112E-6	1.3283589831704223E-5
10-11	0.0	8.634333390607745E-5	0.0	1.3283589831704223E-5	1.9925384747556334E-5
12-13	0.0	9.962692373778167E-5	0.0	2.3246282205482392E-5	1.9925384747556334E-5
14-15	0.0	1.19552308485338E-4	0.0	2.6567179663408447E-5	2.98880771213345E-5
16-17	0.0	1.19552308485338E-4	0.0	2.6567179663408447E-5	4.649256441096478E-5
18-19	0.0	1.2287320594326406E-4	0.0	2.98880771213345E-5	5.9776154242669E-5
20-21	0.0	1.3947769323289434E-4	0.0	5.9776154242669E-5	5.9776154242669E-5
22-23	0.0	1.5940307798045067E-4	0.0	1.361567957749683E-4	7.638064153229929E-5
24-25	0.0	1.8929115510178516E-4	0.0	2.623508991761584E-4	8.634333390607745E-5
26-27	0.0	2.1253743730726757E-4	0.0	5.745152602212076E-4	8.634333390607745E-5
28-29	6.641794915852112E-6	2.1253743730726757E-4	0.0	0.0025205611705658762	8.634333390607745E-5
30-31	6.641794915852112E-6	2.590300017182323E-4	0.0	0.009069370957596058	8.96642313640035E-5
32-33	6.641794915852112E-6	2.723135915499366E-4	0.0	0.023428931565668325	9.298512882192956E-5
34-35	6.641794915852112E-6	3.0220166867127107E-4	0.0	0.0436664806742697	9.962692373778167E-5
36-37	6.641794915852112E-6	3.3208974579260555E-4	0.0	0.07162843727000709	9.962692373778167E-5
38-39	6.641794915852112E-6	3.520151305401619E-4	0.0	0.11257510292623536	9.962692373778167E-5
40-41	6.641794915852112E-6	3.6861961782979214E-4	0.0	0.16631054469293688	1.0294782119570772E-4
42-43	6.641794915852112E-6	3.819032076614964E-4	0.0	0.19872250388229518	1.0626871865363379E-4
44-45	6.641794915852112E-6	4.1511218224075697E-4	0.0	0.24702163651037173	1.0626871865363379E-4
46-47	6.641794915852112E-6	4.4167936190416543E-4	0.0	0.3015540936669755	1.0626871865363379E-4
48-49	6.641794915852112E-6	4.5164205427794356E-4	0.0	0.3444268798488009	1.0626871865363379E-4
50-51	6.641794915852112E-6	5.147391059785386E-4	0.0	0.3846296644744537	1.0626871865363379E-4
52-53	6.641794915852112E-6	5.213809008943907E-4	0.0	0.42633017385363114	1.0626871865363379E-4
54-55	6.641794915852112E-6	5.37985388184021E-4	0.0	0.5177146301008404	1.0958961611155984E-4
56-57	6.641794915852112E-6	5.446271830998732E-4	0.0	0.5963601236994452	1.1291051356948589E-4
58-59	6.641794915852112E-6	5.678734653053555E-4	0.0	0.6599353846339817	1.1291051356948589E-4
60-61	6.641794915852112E-6	5.811570551370597E-4	0.0	0.696428726799131	1.19552308485338E-4
62-63	6.641794915852112E-6	6.044033373425421E-4	0.0	0.7275455359798981	1.19552308485338E-4
64-65	6.641794915852112E-6	6.641794915852111E-4	0.0	0.743140470442319	1.19552308485338E-4
66-67	6.641794915852112E-6	6.641794915852111E-4	0.0	0.7519541322956547	1.2287320594326406E-4
68-69	6.641794915852112E-6	6.708212865010632E-4	0.0	0.7601501072218162	1.261941034011901E-4
70-71	6.641794915852112E-6	6.807839788748413E-4	0.0	0.7681269029157546	1.261941034011901E-4
72-73	6.641794915852112E-6	6.841048763327675E-4	0.0	0.7753398921943699	1.3283589831704224E-4
74-75	6.641794915852112E-6	6.973884661644717E-4	0.0	0.7818123213398679	1.3283589831704224E-4
76-77	6.641794915852112E-6	7.405601331175104E-4	0.0	0.7875973247115751	1.3283589831704224E-4
78-79	6.641794915852112E-6	7.505228254912886E-4	0.0	0.7930635219273214	1.3283589831704224E-4
80-81	6.641794915852112E-6	7.505228254912886E-4	0.0	0.7959028892538482	1.361567957749683E-4
82-83	6.641794915852112E-6	7.505228254912886E-4	0.0	0.7976762484963806	1.3947769323289434E-4
84-85	6.641794915852112E-6	7.505228254912886E-4	0.0	0.7992935255583906	1.3947769323289434E-4
86-87	6.641794915852112E-6	7.505228254912886E-4	0.0	0.799499421200782	1.3947769323289434E-4
88-89	6.641794915852112E-6	7.903735949864012E-4	0.0	0.7995724809448564	1.3947769323289434E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15155	0.0	43.314297	1
GTATCAA	25485	0.0	32.03022	1
TCAACGC	30905	0.0	25.6621	4
ATCAACG	31325	0.0	25.370913	3
CAACGCA	31695	0.0	25.061588	5
AACGCAG	32970	0.0	24.067295	6
TATCAAC	33545	0.0	23.926762	2
GTACATG	38380	0.0	20.394579	1
ACGCAGA	38950	0.0	20.382872	7
TACATGG	38115	0.0	20.003952	2
CGCAGAG	40160	0.0	19.779058	8
ACATGGG	38955	0.0	18.94509	3
CATGGGG	28875	0.0	17.268583	4
AGAGTAC	42370	0.0	16.838388	10-11
GCAGAGT	47955	0.0	16.469011	9
TATACCG	1110	0.0	15.670291	5
GAGTACT	27360	0.0	15.607823	12-13
CAGAGTA	46200	0.0	15.442479	10-11
GTGGTAT	8685	0.0	14.925622	1
TATACTG	8145	0.0	14.84713	5
>>END_MODULE