FastQCFastQC Report
Fri 27 May 2016
SRR2139573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14430159
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144600.043.5073971
GTATCAA248600.031.74531
TCAACGC295950.025.645574
ATCAACG298750.025.4329033
CAACGCA303200.025.0186165
AACGCAG311600.024.4238176
TATCAAC319550.024.1402722
GTACATG370400.021.6860491
TACATGG378250.020.853262
ACGCAGA369550.020.5155127
ACATGGG378200.020.2541543
CGCAGAG384950.019.727028
CATGGGG278050.017.5679744
AGAGTAC397600.016.7027210-11
GCAGAGT456200.016.5644269
GAGTACT257700.016.32412112-13
GTGGTAT83950.016.259061
CAGAGTA439650.015.55680410-11
GAGTACA274950.014.8930491
TGGTATC82800.014.8863462
Produced by FastQC (version 0.11.5)