##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139573_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14430159
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15575005098697	33.0	31.0	34.0	30.0	34.0
2	32.286470578737216	34.0	31.0	34.0	30.0	34.0
3	32.33846155125526	34.0	31.0	34.0	30.0	34.0
4	35.96436955407075	37.0	35.0	37.0	35.0	37.0
5	35.82994393894066	37.0	35.0	37.0	35.0	37.0
6	35.84911545326701	37.0	35.0	37.0	35.0	37.0
7	35.763143496894244	37.0	35.0	37.0	33.0	37.0
8	35.72908365042963	37.0	35.0	37.0	33.0	37.0
9	37.36022395872423	39.0	37.0	39.0	34.0	39.0
10-11	37.2590476307295	39.0	37.0	39.0	33.5	39.0
12-13	37.216807209123616	39.0	37.0	39.0	33.0	39.0
14-15	38.41459726812435	40.0	38.0	41.0	33.0	41.0
16-17	38.43080315331245	40.0	38.0	41.0	33.0	41.0
18-19	38.423716606310435	40.0	38.0	41.0	33.5	41.0
20-21	38.39110400986543	40.0	38.0	41.0	34.0	41.0
22-23	38.28859172388645	40.0	38.0	41.0	33.5	41.0
24-25	38.19882683238038	40.0	38.0	41.0	33.0	41.0
26-27	38.02501707289176	40.0	37.0	41.0	33.0	41.0
28-29	37.88880195111413	40.0	37.0	41.0	33.0	41.0
30-31	37.73726871496724	40.0	37.0	41.0	32.5	41.0
32-33	37.93035273072176	40.0	37.0	41.0	33.0	41.0
34-35	37.910722734491074	40.0	37.0	41.0	33.0	41.0
36-37	37.82566023298388	40.0	37.0	41.0	33.0	41.0
38-39	37.712177753506936	40.0	37.0	41.0	33.0	41.0
40-41	37.658790639513875	40.0	37.0	41.0	33.0	41.0
42-43	37.54531347252096	40.0	37.0	41.0	32.0	41.0
44-45	37.58714821906227	39.5	36.5	41.0	32.5	41.0
46-47	37.72642008085943	40.0	37.0	41.0	33.0	41.0
48-49	37.64830892372066	40.0	37.0	41.0	32.0	41.0
50-51	37.5185938352445	40.0	36.0	41.0	32.0	41.0
52-53	37.36221309178649	39.0	36.0	41.0	31.0	41.0
54-55	37.248643624307576	39.0	36.0	41.0	31.0	41.0
56-57	37.069466408196284	39.0	35.0	40.5	31.0	41.0
58-59	36.911663813505015	39.0	35.0	40.0	31.0	41.0
60-61	36.67199502539175	38.0	35.0	40.0	31.0	41.0
62-63	36.39946999238538	38.0	35.0	40.0	30.0	41.0
64-65	36.16698268928174	37.5	34.5	40.0	30.0	41.0
66-67	35.866680206955415	37.0	34.0	40.0	30.0	41.0
68-69	35.53771415515119	36.5	34.0	39.0	29.0	41.0
70-71	35.22920904889922	36.0	34.0	39.0	29.0	40.5
72-73	34.91555013145209	36.0	34.0	39.0	29.0	40.0
74-75	34.52915999143603	35.0	33.0	38.0	29.0	40.0
76-77	33.46927316639459	34.5	31.5	36.5	27.5	39.0
78-79	33.80620541004563	35.0	33.0	37.0	28.5	39.0
80-81	33.68721867570447	35.0	33.0	36.0	29.0	38.5
82-83	33.46403837175079	35.0	33.0	36.0	29.0	37.0
84-85	33.24823875575899	35.0	32.5	35.5	28.0	37.0
86-87	33.0423245973763	34.5	32.0	35.0	28.0	36.5
88-89	32.81628165042498	34.0	32.0	35.0	27.0	36.0
90-91	32.67553007276018	34.0	32.0	35.0	27.0	36.0
92-93	32.58601863362948	34.0	32.0	35.0	27.0	35.5
94-95	32.53753059163206	34.0	32.0	35.0	27.0	35.0
96-97	32.47623280652613	34.0	32.0	35.0	27.0	35.0
98-99	32.608875841119186	34.0	32.0	35.0	28.5	35.0
100-101	31.66844647432456	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	14.0
20	111.0
21	650.0
22	2918.0
23	10023.0
24	25529.0
25	54106.0
26	94943.0
27	148802.0
28	209487.0
29	275783.0
30	347908.0
31	428611.0
32	531637.0
33	672748.0
34	892725.0
35	1232367.0
36	1869592.0
37	2943839.0
38	3113872.0
39	1514153.0
40	60340.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.42507494200168	21.329917431956225	14.079186514854063	25.165821111188038
2	14.845670317744613	22.208068823356363	39.570616324271995	23.37564453462703
3	17.758030247622358	26.29916967650876	30.97581253262698	24.9669875432419
4	12.584309809930788	18.213406798228593	38.00066832468106	31.201615067159562
5	12.970771839728169	37.97536118624888	35.96459332152889	13.089273652494057
6	30.834566687726728	37.96891635081776	18.00847793846208	13.18803902299344
7	26.476361071281335	32.41172879661271	23.382389618853125	17.729520513252833
8	24.66778086090389	36.9057957019046	21.04962253014676	17.376800907044753
9	25.559392658112777	15.854565427865348	21.05527042356221	37.53077149045967
10-11	24.391221191672248	26.56976267551868	29.136397596173403	19.902618536635668
12-13	24.78803941106955	24.178399558868342	29.166833851241698	21.866727178820415
14-15	21.956549473917786	25.715118592941355	26.332675890820052	25.995656042320807
16-17	21.87733600708346	28.650799753189098	27.2300327301306	22.24183150959684
18-19	21.933358461261584	28.01521452397025	28.607207307972143	21.444219706796023
20-21	22.512930803718774	27.296800603913972	28.45968844789293	21.730580144474324
22-23	22.313350899774733	27.269758590463233	28.321953405657613	22.09493710410442
24-25	22.127918751743124	27.448088260200628	28.382264213651037	22.041728774405215
26-27	22.1261252951242	27.52951848872828	28.337582728838402	22.00677348730912
28-29	22.072493458164917	27.556727676381325	28.34168544010079	22.029093425352965
30-31	22.320972654300952	27.479147769101758	28.241684593845417	21.958194982751873
32-33	22.107102841094655	27.608516111420993	28.222319016790028	22.062062030694324
34-35	22.235914158743856	27.555475490201047	28.248000432408816	21.960609918646277
36-37	22.178076587428418	27.5146916683523	28.207170668134985	22.100061076084305
38-39	22.052206003920777	27.55903675934342	28.302557817022656	22.086199419713147
40-41	22.236787755039046	27.45269955194799	28.21692251316734	22.093590179845616
42-43	22.16767549711051	27.535965031920846	28.03841600659039	22.25794346437826
44-45	22.16597641456957	27.64691723255348	27.9093716136176	22.277734739259344
46-47	22.285391993456955	27.54459255625863	27.709924713544687	22.460090736739726
48-49	22.215588082400092	27.65430375707303	27.680623721640767	22.449484438886113
50-51	22.21133438662285	27.734170273917425	27.62842572755116	22.426069611908567
52-53	22.305798196463094	27.664434186764925	27.571780140529462	22.457987476242515
54-55	22.298949745823514	27.580772796974905	27.558773708845504	22.561503748356078
56-57	22.323353976309992	27.690031505941405	27.468984898189895	22.517629619558708
58-59	22.223527460870386	27.715389321748958	27.5681675085521	22.49291570882856
60-61	22.3432084483159	27.73845626667259	27.47609177767635	22.44224350733517
62-63	22.273760635596528	27.880236735440494	27.428456458645545	22.417546170317433
64-65	22.235942335405078	27.76794137771949	27.427859320004355	22.568256966871076
66-67	22.327381708923905	27.80955486322872	27.244860643614828	22.61820278423255
68-69	22.269140529428704	27.77424737519117	27.289960597706674	22.666651497673456
70-71	22.255595125787295	27.606889319919926	27.380782705631113	22.756732848661667
72-73	22.30648998747278	27.60068137707662	27.313944561011045	22.778884074439556
74-75	22.26101930240522	27.705380921024148	27.275931871117287	22.757667905453346
76-77	22.468036851715645	27.634275211155884	27.201314923310072	22.6963730138184
78-79	22.392792381400977	27.57908305611406	27.1879190453065	22.840205517178454
80-81	22.367259207967056	27.676342128430253	27.17051723774456	22.785881425858133
82-83	22.42009155367171	27.600186908042712	27.30203582168392	22.67768571660166
84-85	22.44767687294542	27.63855596888706	27.231372413878635	22.682394744288878
86-87	22.391131516628793	27.6233992537112	27.31006957805149	22.675399651608515
88-89	22.382724118216597	27.6197748760366	27.322979954229048	22.67452105151775
90-91	22.41418067730579	27.652556736090343	27.33535129717819	22.597911289425674
92-93	22.484292206719108	27.504931374542107	27.40459476887499	22.606181649863796
94-95	22.239560014531804	27.49782009742219	27.551942362452436	22.710677525593574
96-97	22.17251678009221	27.55223312957949	27.615282717739255	22.659967372589044
98-99	22.311559962683287	28.11543380680958	27.716790935556574	21.856215294950566
100-101	25.24297934927011	35.87653057579231	28.87903894959638	10.0014511253412
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2943.0
1	2717.0
2	3601.5
3	4960.0
4	6724.5
5	10479.5
6	13565.5
7	15897.5
8	17336.0
9	17050.5
10	15928.5
11	14883.5
12	16377.0
13	21887.0
14	34241.5
15	50277.5
16	62240.0
17	67832.5
18	68995.5
19	67867.5
20	65172.0
21	63069.5
22	64451.5
23	71609.0
24	84419.5
25	103156.5
26	126360.0
27	152170.0
28	182326.5
29	215462.0
30	247869.0
31	278671.0
32	309761.5
33	341418.5
34	372760.0
35	404010.5
36	437992.0
37	467813.0
38	493338.0
39	518033.5
40	539039.0
41	557800.0
42	571660.5
43	584188.0
44	597750.0
45	611621.0
46	625231.0
47	633205.5
48	634518.0
49	631662.5
50	623993.5
51	609375.0
52	584241.5
53	551186.5
54	515269.5
55	478744.5
56	441660.5
57	400763.0
58	358415.5
59	311454.5
60	260811.5
61	214231.5
62	172842.5
63	135683.5
64	102383.5
65	76710.0
66	58525.5
67	45310.5
68	34876.5
69	25955.0
70	19028.5
71	13842.5
72	9644.0
73	6517.5
74	4508.0
75	3066.5
76	2103.5
77	1434.5
78	997.5
79	708.5
80	508.5
81	371.5
82	272.0
83	191.0
84	131.5
85	94.0
86	71.5
87	52.5
88	34.5
89	24.0
90	18.0
91	15.5
92	13.5
93	8.5
94	3.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001205807919372198
3	0.0
4	3.257067368419156E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.003385271083984591
18-19	0.0
20-21	0.0021795593783356096
22-23	0.0
24-25	2.8077090539569923E-4
26-27	0.003328895384087776
28-29	0.0
30-31	1.3198971049897912E-4
32-33	7.764396052023961E-4
34-35	8.315485029838441E-4
36-37	0.01016192088335158
38-39	0.011614011528401087
40-41	0.025032635073069995
42-43	0.0018283149739140043
44-45	0.0010769586296676865
46-47	0.001752309061274472
48-49	0.0017936685944527537
50-51	7.48818944040985E-5
52-53	1.0584702360626782E-4
54-55	0.006285603871931985
56-57	0.0037113001953833664
58-59	0.0035786541747049793
60-61	0.0016117776066098051
62-63	4.399048573774464E-4
64-65	6.803538747287089E-4
66-67	6.65264491708977E-4
68-69	1.2501915918614527E-5
70-71	0.0
72-73	1.3304440095084687E-4
74-75	2.482735947137412E-4
76-77	0.001468435081214854
78-79	0.002076432771196839
80-81	0.011399207185826099
82-83	0.010100985192681527
84-85	0.01295431264308256
86-87	0.006621544423671664
88-89	0.08802674990887684
90-91	3.3138883403399476E-4
92-93	4.626173326060304E-5
94-95	2.3744722075943008E-4
96-97	0.014612043687608047
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	384.0
20-21	1320.0
22-23	2584.0
24-25	5138.0
26-27	6410.0
28-29	12192.0
30-31	31576.0
32-33	45177.0
34-35	65500.0
36-37	99875.0
38-39	102216.0
40-41	108036.0
42-43	125205.0
44-45	147301.0
46-47	149268.0
48-49	140196.0
50-51	131044.0
52-53	121156.0
54-55	138712.0
56-57	146259.0
58-59	155040.0
60-61	155872.0
62-63	152622.0
64-65	166146.0
66-67	178758.0
68-69	175962.0
70-71	174083.0
72-73	169716.0
74-75	174799.0
76-77	175719.0
78-79	189068.0
80-81	190575.0
82-83	182250.0
84-85	186001.0
86-87	199403.0
88-89	210675.0
90-91	229028.0
92-93	240473.0
94-95	302804.0
96-97	385943.0
98-99	1211174.0
100-101	7644499.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.08112862010829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65643860849657	75.38414546261843
2	6.991167047758752	11.756504314945328
3	1.7968646564814308	4.532472248836257
4	0.6882109578996669	2.3146221627571935
5	0.31266741845646256	1.3144714713277525
6	0.1817191993771028	0.9167493225341569
7	0.09941347186069194	0.5851157839863248
8	0.0697828011045362	0.4693933340113555
9	0.04584894065263586	0.34695276084989884
>10	0.1529021727135792	2.0201129913857563
>50	0.003983336457006219	0.2199290347482866
>100	9.863326082383504E-4	0.13149241406789142
>500	1.505613331885249E-5	0.008038697931359981
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.929930571104587E-6
2	1.3859861142209174E-5	1.3859861142209174E-5	0.0	6.929930571104587E-6	6.929930571104587E-6
3	1.3859861142209174E-5	1.3859861142209174E-5	0.0	6.929930571104587E-6	6.929930571104587E-6
4	1.3859861142209174E-5	2.078979171331376E-5	0.0	2.078979171331376E-5	6.929930571104587E-6
5	1.3859861142209174E-5	2.078979171331376E-5	0.0	2.078979171331376E-5	6.929930571104587E-6
6	4.157958342662752E-5	2.078979171331376E-5	0.0	2.7719722284418348E-5	6.929930571104587E-6
7	4.157958342662752E-5	2.078979171331376E-5	0.0	2.7719722284418348E-5	6.929930571104587E-6
8	4.157958342662752E-5	2.078979171331376E-5	0.0	3.464965285552294E-5	1.3859861142209174E-5
9	5.5439444568836695E-5	5.5439444568836695E-5	0.0	6.929930571104588E-5	2.7719722284418348E-5
10-11	5.5439444568836695E-5	5.5439444568836695E-5	0.0	9.008909742435963E-5	2.7719722284418348E-5
12-13	5.890440985438899E-5	5.5439444568836695E-5	0.0	1.1434385442322568E-4	2.7719722284418348E-5
14-15	6.583434042549359E-5	1.0048399328101653E-4	0.0	1.2127378499433028E-4	4.8509513997732105E-5
16-17	8.315916685325504E-5	1.0394895856656881E-4	0.0	1.4206357670764404E-4	6.236937513994129E-5
18-19	8.315916685325504E-5	1.074139238521211E-4	0.0	1.9750302127648074E-4	6.583434042549359E-5
20-21	8.315916685325504E-5	1.2473875027988257E-4	0.0	3.638213549829908E-4	6.929930571104588E-5
22-23	9.701902799546422E-5	1.3166868085098715E-4	0.0	7.276427099659816E-4	8.662413213880735E-5
24-25	1.1087888913767339E-4	1.3859861142209175E-4	0.0	0.001451820454646411	1.0394895856656881E-4
26-27	1.2473875027988257E-4	1.4552854199319633E-4	0.0	0.003922340703245196	1.0394895856656881E-4
28-29	1.2473875027988257E-4	1.4552854199319633E-4	0.0	0.02060614855317949	1.0394895856656881E-4
30-31	1.2473875027988257E-4	1.697832989920624E-4	0.0	0.03363095306157056	1.074139238521211E-4
32-33	1.2473875027988257E-4	1.7671322956316698E-4	0.0	0.047099273126512325	1.2473875027988257E-4
34-35	1.2473875027988257E-4	2.0443295184758534E-4	0.0	0.06665207223288391	1.3166868085098715E-4
36-37	1.2473875027988257E-4	2.2868770884645139E-4	0.0	0.09440990913544334	1.3166868085098715E-4
38-39	1.2473875027988257E-4	2.3561763941755596E-4	0.0	0.13585782388121986	1.3166868085098715E-4
40-41	1.2473875027988257E-4	2.841271534152881E-4	0.0	0.19405191585207066	1.3859861142209175E-4
42-43	1.3166868085098715E-4	3.014519798430496E-4	0.0	0.2282441932899007	1.3859861142209175E-4
44-45	1.3166868085098715E-4	3.4303156326967704E-4	0.0	0.27858667392368996	1.3859861142209175E-4
46-47	1.3859861142209175E-4	3.672863202685431E-4	0.0	0.3356927667948773	1.4899350727874862E-4
48-49	1.3859861142209175E-4	3.672863202685431E-4	0.0	0.3805398124857806	1.593884031354055E-4
50-51	1.4899350727874862E-4	3.880761119818569E-4	0.0	0.4241325407433141	1.593884031354055E-4
52-53	1.5245847256430093E-4	3.9500604255296147E-4	0.0	0.4691077901497828	1.663183337065101E-4
54-55	1.5245847256430093E-4	3.9847100783851376E-4	0.0	0.5728142011463631	1.8017819484871927E-4
56-57	1.663183337065101E-4	4.088659036951706E-4	0.0	0.6625117574934551	1.8710812541982384E-4
58-59	1.8017819484871927E-4	4.123308689807229E-4	0.0	0.7375559756479468	1.8710812541982384E-4
60-61	1.8710812541982384E-4	4.331206606940367E-4	0.0	0.7752651928506125	1.9403805599092845E-4
62-63	2.0096798656203302E-4	4.36585625979589E-4	0.0	0.7973751363377215	2.182928129897945E-4
64-65	2.0096798656203302E-4	5.058849316906349E-4	0.0	0.8136119636658197	2.2175777827534678E-4
66-67	2.148278477042422E-4	5.093498969761872E-4	0.0	0.8229812297979531	2.2522274356089907E-4
68-69	2.2522274356089907E-4	5.301396886895008E-4	0.0	0.831532764122696	2.2868770884645139E-4
70-71	2.3908260470310828E-4	5.336046539750532E-4	0.0	0.8401501327878647	2.3215267413200367E-4
72-73	2.841271534152881E-4	5.370696192606055E-4	0.0	0.8473815153388122	2.4947750055976514E-4
74-75	2.9105708398639266E-4	5.78649202687233E-4	0.0	0.8539372296590773	2.4947750055976514E-4
76-77	2.9105708398639266E-4	6.306236819705175E-4	0.0	0.8596890720330941	2.4947750055976514E-4
78-79	2.9452204927194495E-4	6.514134736838312E-4	0.0	0.8649870039547035	2.4947750055976514E-4
80-81	2.9798701455749723E-4	6.548784389693834E-4	0.0	0.8681920968438394	2.5294246584531743E-4
82-83	2.9798701455749723E-4	6.583434042549358E-4	0.0	0.8702294964317441	2.9105708398639266E-4
84-85	2.9798701455749723E-4	6.583434042549358E-4	0.0	0.8718060556366705	2.9105708398639266E-4
86-87	2.9798701455749723E-4	6.583434042549358E-4	0.0	0.8720243484496601	2.9452204927194495E-4
88-89	2.9798701455749723E-4	6.929930571104588E-4	0.0	0.8720693929983724	3.0838191041415415E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14460	0.0	43.507397	1
GTATCAA	24860	0.0	31.7453	1
TCAACGC	29595	0.0	25.64557	4
ATCAACG	29875	0.0	25.432903	3
CAACGCA	30320	0.0	25.018616	5
AACGCAG	31160	0.0	24.423817	6
TATCAAC	31955	0.0	24.140272	2
GTACATG	37040	0.0	21.686049	1
TACATGG	37825	0.0	20.85326	2
ACGCAGA	36955	0.0	20.515512	7
ACATGGG	37820	0.0	20.254154	3
CGCAGAG	38495	0.0	19.72702	8
CATGGGG	27805	0.0	17.567974	4
AGAGTAC	39760	0.0	16.70272	10-11
GCAGAGT	45620	0.0	16.564426	9
GAGTACT	25770	0.0	16.324121	12-13
GTGGTAT	8395	0.0	16.25906	1
CAGAGTA	43965	0.0	15.556804	10-11
GAGTACA	27495	0.0	14.893049	1
TGGTATC	8280	0.0	14.886346	2
>>END_MODULE