FastQCFastQC Report
Fri 27 May 2016
SRR2139572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8499642
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88550.047.7065431
GTATCAA159900.035.4363981
TCAACGC189200.029.3692654
ATCAACG192500.028.8000873
CAACGCA193350.028.7170895
AACGCAG199300.027.878946
TATCAAC204400.027.4946422
ACGCAGA231400.024.0115497
CGCAGAG242600.022.8856458
GTACATG232600.022.7112051
TACATGG238350.022.021812
ACATGGG240350.021.154463
CATGGGG178600.019.3094844
CGAGACC106800.019.29935686-87
GCAGAGT289950.019.2355619
ACCGAGG114900.018.54255390-91
AGACCGA113500.018.5216888-89
GACCGAG113750.018.34462490-91
GAGACCG112500.018.25234488-89
GAGTACT163950.018.06354712-13
Produced by FastQC (version 0.11.5)