##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139572_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8499642 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.13458990390419 28.0 18.0 31.0 18.0 33.0 2 31.573965115236618 33.0 31.0 33.0 27.0 33.0 3 31.93932403270632 33.0 31.0 33.0 29.0 34.0 4 32.62848117603071 33.0 33.0 34.0 31.0 34.0 5 32.991294692176446 34.0 33.0 34.0 32.0 34.0 6 37.08118106621432 38.0 38.0 38.0 36.0 38.0 7 37.16888640721574 38.0 38.0 38.0 36.0 38.0 8 37.260111072913425 38.0 38.0 38.0 36.0 38.0 9 37.351137142011396 38.0 38.0 38.0 37.0 38.0 10-11 37.37008341057188 38.0 38.0 38.0 37.0 38.0 12-13 37.394019183396196 38.0 38.0 38.0 37.0 38.0 14-15 37.3940250659969 38.0 38.0 38.0 37.0 38.0 16-17 37.38500686264198 38.0 38.0 38.0 37.0 38.0 18-19 37.2648505666474 38.0 38.0 38.0 37.0 38.0 20-21 37.380191700656994 38.0 38.0 38.0 37.0 38.0 22-23 37.40029047401729 38.0 38.0 38.0 37.0 38.0 24-25 37.40809649158296 38.0 38.0 38.0 37.0 38.0 26-27 37.38192168408906 38.0 38.0 38.0 37.0 38.0 28-29 37.359138828168994 38.0 38.0 38.0 37.0 38.0 30-31 37.36283567244324 38.0 38.0 38.0 37.0 38.0 32-33 37.35110239768595 38.0 38.0 38.0 37.0 38.0 34-35 37.333470710792106 38.0 38.0 38.0 37.0 38.0 36-37 37.31075198289241 38.0 38.0 38.0 37.0 38.0 38-39 37.27644314474877 38.0 38.0 38.0 37.0 38.0 40-41 37.28939784279645 38.0 38.0 38.0 37.0 38.0 42-43 37.277547367899615 38.0 38.0 38.0 37.0 38.0 44-45 37.25262121598091 38.0 38.0 38.0 37.0 38.0 46-47 37.22543725176422 38.0 38.0 38.0 37.0 38.0 48-49 37.2011965805677 38.0 38.0 38.0 37.0 38.0 50-51 37.1748410208124 38.0 38.0 38.0 37.0 38.0 52-53 37.15430220277136 38.0 38.0 38.0 36.5 38.0 54-55 37.139874084645626 38.0 38.0 38.0 36.0 38.0 56-57 37.12816528266709 38.0 38.0 38.0 36.0 38.0 58-59 37.11634791993126 38.0 38.0 38.0 36.0 38.0 60-61 37.11260639272484 38.0 38.0 38.0 36.0 38.0 62-63 37.10487695170559 38.0 38.0 38.0 36.0 38.0 64-65 37.10830651246246 38.0 38.0 38.0 36.0 38.0 66-67 37.09633403873475 38.0 38.0 38.0 36.0 38.0 68-69 37.096668838504335 38.0 38.0 38.0 36.0 38.0 70-71 37.088261725580224 38.0 38.0 38.0 36.0 38.0 72-73 37.060022619993674 38.0 38.0 38.0 36.0 38.0 74-75 37.077395246191514 38.0 38.0 38.0 36.0 38.0 76-77 37.06423884435708 38.0 38.0 38.0 36.0 38.0 78-79 37.0525068371634 38.0 38.0 38.0 36.0 38.0 80-81 37.027349552288754 38.0 38.0 38.0 36.0 38.0 82-83 37.01544713726926 38.0 38.0 38.0 36.0 38.0 84-85 37.00184301767024 38.0 38.0 38.0 36.0 38.0 86-87 36.96839319189792 38.0 38.0 38.0 36.0 38.0 88-89 36.94984259549089 38.0 38.0 38.0 36.0 38.0 90-91 36.95547986438051 38.0 38.0 38.0 36.0 38.0 92-93 36.92366982526174 38.0 38.0 38.0 35.0 38.0 94-95 36.85788215129999 38.0 38.0 38.0 35.0 38.0 96-97 36.86928114491943 38.0 38.0 38.0 35.0 38.0 98-99 36.895443248650096 38.0 38.0 38.0 35.0 38.0 100 35.35368479976168 38.0 35.0 38.0 29.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 4.0 18 14.0 19 25.0 20 89.0 21 187.0 22 634.0 23 1629.0 24 3714.0 25 7348.0 26 13344.0 27 21561.0 28 32669.0 29 48143.0 30 66267.0 31 89069.0 32 117327.0 33 159184.0 34 233731.0 35 406435.0 36 1185077.0 37 6113191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59359394195661 19.016236213242863 13.85039511075878 24.53977473404174 2 15.420061221402031 20.892891724145557 40.03407437630903 23.652972678143385 3 18.633773045970642 25.406399469530598 30.429775748202097 25.53005173629666 4 12.740795435854826 16.985750693970406 37.96618728177022 32.307266588404545 5 13.436467741622362 37.48632381741245 35.527930944616976 13.549277496348214 6 31.149094765519546 37.054958097543405 18.177492855269666 13.618454281667383 7 27.07785270905591 32.097171174308556 22.90394652008791 17.92102959654762 8 25.117552009837592 36.09957925286736 21.011332006689223 17.77153673060583 9 26.032543488302213 15.72834479381602 20.83703054787484 37.40208117000692 10-11 24.78022015515477 26.09978161433152 28.84114413289407 20.278854097619643 12-13 25.21420462263026 23.758454988954533 28.876894396075407 22.150445992339797 14-15 22.38287212567306 25.119316790048334 26.156107516057737 26.341703568220876 16-17 22.262467054494767 28.069894002594463 26.987554299345785 22.68008464356499 18-19 22.295168431799834 27.31002670465415 28.423920678070914 21.970884185475107 20-21 22.924401158416313 26.662570364143452 28.309054554160905 22.103973923279334 22-23 22.753197972344257 26.67211974527644 28.137450290025225 22.437231992354082 24-25 22.549156827829272 26.741463775669622 28.31811933990701 22.39126005659409 26-27 22.5014499369159 26.854891043058466 28.28350475156257 22.360154268463063 28-29 22.46118853395687 26.769421507109314 28.306718189859865 22.462671769073946 30-31 22.710986623177064 26.77019014130978 28.199631878128567 22.31919135738459 32-33 22.543074809105143 26.852037607116557 28.268987731506545 22.33589985227176 34-35 22.522113438966198 26.790976852320657 28.394283342186405 22.292626366526736 36-37 22.630149528621953 26.715277214738016 28.22723106360711 22.42734219303292 38-39 22.38675285009864 26.86039322013931 28.379504012469337 22.37334991729271 40-41 22.637872242157417 26.678853169454943 28.3035233468372 22.37975124155044 42-43 22.525353415195095 26.75536595159477 28.215951770719432 22.503328862490708 44-45 22.453363163390456 26.944032126031992 28.091536339081042 22.511068371496513 46-47 22.67848893061317 26.792101316895877 27.827247149557717 22.70216260293324 48-49 22.57994408538671 26.84469858263885 27.843578944114306 22.731778387860135 50-51 22.472882497857196 27.032154734957338 27.821317959940995 22.67364480724447 52-53 22.620196997577207 26.94772197100548 27.780606022408953 22.651475009008358 54-55 22.647245180912776 26.93411489852248 27.693314945068575 22.725324975496168 56-57 22.64568338859952 27.083082907924684 27.604367793353568 22.666865910122233 58-59 22.445689804678043 27.112696564152607 27.734301973812336 22.707311657357014 60-61 22.778304346712464 27.158366540584904 27.699914794748494 22.363414317954135 62-63 22.438647332254934 27.31494154398052 27.653858158159807 22.592552965604735 64-65 22.382908609748934 27.262662993945302 27.623067606514688 22.731360789791072 66-67 22.43590545198667 27.256114233067336 27.74481351010361 22.56316680484238 68-69 22.398096047449947 27.440836533964063 27.580008470859376 22.581058947726614 70-71 22.43169176137869 27.115880218986483 27.4555640475743 22.996863972060527 72-73 22.5733762782835 27.022806031016 27.389349700077986 23.01446799062251 74-75 22.456410009337127 27.19797298002065 27.61514577135457 22.730471239287652 76-77 22.643282905649514 26.850086638945648 27.510513062719017 22.996117392685818 78-79 22.503991470147692 26.859711405889165 27.223249598384186 23.413047525578957 80-81 22.87852973161154 27.139325392397406 27.196580872391507 22.785564003599546 82-83 22.92714232459714 26.85881681522141 27.259814174496206 22.954226685685246 84-85 22.707338444028256 26.870765761154303 27.165370926850418 23.256524867967023 86-87 22.587872810677055 27.213672344207446 27.24066460990273 22.957790235212766 88-89 22.94884930658319 27.1858880409781 27.156181137018066 22.709081515420642 90-91 22.978463213893484 27.2201615020752 27.125191521492503 22.67618376253881 92-93 22.85259107256807 26.876696428465465 27.117417674386218 23.153294824580247 94-95 22.908897244685072 27.206946094745817 27.229482212060613 22.654674448508498 96-97 23.29119181469645 26.98032294217893 27.289466823498888 22.439018419625732 98-99 23.9939539582985 29.09846563237078 27.515546456155082 19.392033953175638 100 30.780924763231752 40.31996415012996 28.899111086638285 0.0 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1577.0 1 1567.0 2 2223.0 3 3178.0 4 3959.0 5 5227.0 6 6491.5 7 8120.0 8 9650.0 9 10232.5 10 9922.5 11 9656.5 12 10631.0 13 12834.5 14 16771.0 15 21029.0 16 23855.0 17 25016.5 18 24732.0 19 23579.0 20 22516.0 21 22415.0 22 24534.5 23 29489.5 24 37105.0 25 47277.0 26 60401.5 27 75227.0 28 90535.0 29 108085.0 30 125580.0 31 142071.5 32 160491.0 33 180896.0 34 199176.5 35 217817.5 36 239173.0 37 257468.5 38 274032.0 39 287990.0 40 300086.5 41 311743.0 42 321642.0 43 331480.0 44 341757.5 45 352862.5 46 368618.5 47 379250.5 48 380216.0 49 376797.5 50 367942.5 51 358677.0 52 348592.5 53 336644.5 54 320390.5 55 300592.0 56 279770.0 57 256833.5 58 232324.5 59 201161.0 60 167286.5 61 136681.0 62 108584.5 63 84064.0 64 63733.5 65 48124.0 66 35788.0 67 27129.0 68 20400.5 69 14822.0 70 10734.5 71 7427.0 72 4969.0 73 3148.5 74 1976.0 75 1276.5 76 840.0 77 547.0 78 364.5 79 238.0 80 149.5 81 98.5 82 63.0 83 40.5 84 30.0 85 22.0 86 12.5 87 7.5 88 6.0 89 5.0 90 4.5 91 3.5 92 2.5 93 2.0 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.004435480929667391 6 2.4706922950401913E-4 7 2.353040280990658E-5 8 0.0 9 0.0 10-11 0.0 12-13 5.882600702476645E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 5.313538003338909E-5 34-35 1.0659208063477715E-4 36-37 1.1896937543873675E-4 38-39 1.4965288910289917E-4 40-41 4.821084724778737E-5 42-43 1.27414605366327E-4 44-45 1.2233440218939432E-4 46-47 5.557196986887794E-5 48-49 3.8642462856179106E-4 50-51 2.8929109155125996E-4 52-53 3.804325048379919E-4 54-55 0.0021098745967262416 56-57 0.003329216331509426 58-59 0.001989035345158083 60-61 0.003478312817838203 62-63 0.002117315558822103 64-65 0.002693904109447107 66-67 7.165032532289456E-4 68-69 6.362339764815084E-4 70-71 3.394332960233796E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 72.0 20-21 271.0 22-23 588.0 24-25 640.0 26-27 1953.0 28-29 6168.0 30-31 15894.0 32-33 23279.0 34-35 33329.0 36-37 50896.0 38-39 56037.0 40-41 54987.0 42-43 63025.0 44-45 75546.0 46-47 76168.0 48-49 73135.0 50-51 66351.0 52-53 63704.0 54-55 69894.0 56-57 74454.0 58-59 40834.0 60-61 77022.0 62-63 76054.0 64-65 80632.0 66-67 87000.0 68-69 90058.0 70-71 96321.0 72-73 79605.0 74-75 77506.0 76-77 81353.0 78-79 83777.0 80-81 83621.0 82-83 78083.0 84-85 76922.0 86-87 79206.0 88-89 82123.0 90-91 83582.0 92-93 81463.0 94-95 92162.0 96-97 299414.0 98-99 1925634.0 100-101 3940879.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.62783215347343 #Duplication Level Percentage of deduplicated Percentage of total 1 89.4714585984709 74.82304122199373 2 6.992030109325071 11.69456640789339 3 1.8810952495097313 4.719357533720881 4 0.719707883621082 2.4075044036398174 5 0.339375528587339 1.4190619870849157 6 0.1932565181218027 0.9696974196032887 7 0.11370772354028363 0.6656391293146279 8 0.0707495463068466 0.47333049467866906 9 0.048665612281532995 0.36628196899734505 >10 0.1654106154644394 2.1436452643761266 >50 0.0037364972365731206 0.20666276980058954 >100 7.807871757288079E-4 0.09100259766466845 >500 2.533035863464462E-5 0.020208801231922206 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.353040280990658E-5 0.0 2.353040280990658E-5 0.0 3 0.0 3.5295604214859874E-5 0.0 2.353040280990658E-5 0.0 4 0.0 1.176520140495329E-4 0.0 5.882600702476645E-5 0.0 5 0.0 1.176520140495329E-4 0.0 7.059120842971975E-5 0.0 6 9.412161123962633E-5 1.176520140495329E-4 0.0 7.059120842971975E-5 0.0 7 9.412161123962633E-5 1.176520140495329E-4 0.0 7.059120842971975E-5 0.0 8 9.412161123962633E-5 1.529476182643928E-4 0.0 7.059120842971975E-5 0.0 9 9.412161123962633E-5 2.9413003512383226E-4 0.0 1.176520140495329E-4 0.0 10-11 9.412161123962633E-5 2.9413003512383226E-4 0.0 1.647128196693461E-4 0.0 12-13 9.412161123962633E-5 3.0589523652878553E-4 0.0 1.8236062177677602E-4 0.0 14-15 9.412161123962633E-5 4.058994484708885E-4 0.0 2.0000842388420595E-4 0.0 16-17 1.647128196693461E-4 4.176646498758418E-4 0.0 2.2942142739658917E-4 0.0 18-19 1.647128196693461E-4 4.64725455495655E-4 0.0 2.588344309089724E-4 0.0 20-21 1.647128196693461E-4 5.059036604129915E-4 0.0 2.882474344213556E-4 0.0 22-23 1.647128196693461E-4 5.411992646278513E-4 0.0 3.529560421485987E-4 0.0 24-25 1.647128196693461E-4 5.706122681402346E-4 0.0 4.000168477684119E-4 0.0 26-27 1.647128196693461E-4 5.941426709501411E-4 0.0 4.529602540907017E-4 0.0 28-29 1.647128196693461E-4 6.000252716526178E-4 0.0 7.47090289214534E-4 0.0 30-31 1.647128196693461E-4 7.235598864046274E-4 0.0 0.001741249807933087 0.0 32-33 1.7647802107429936E-4 7.412076885120573E-4 0.0 0.004082524887518792 0.0 34-35 1.7647802107429936E-4 8.823901053714968E-4 0.0 0.007053238242269498 0.0 36-37 1.7647802107429936E-4 0.0010353377236358896 0.0 0.012194631256234087 0.0 38-39 1.7647802107429936E-4 0.0010765159285532262 0.0 0.02500693558622822 0.0 40-41 1.7647802107429936E-4 0.0013059373559498153 0.0 0.038860460240560724 0.0 42-43 1.7647802107429936E-4 0.001376528564379535 0.0 0.04747258766898653 0.0 44-45 1.7647802107429936E-4 0.0014882979777265915 0.0 0.05806126893344449 0.0 46-47 1.7647802107429936E-4 0.0015530065854538345 0.0 0.06965587491802597 0.0 48-49 1.7647802107429936E-4 0.0015647717868587877 0.0 0.08067986863446719 0.0 50-51 1.9412582318172929E-4 0.0017059542037182272 0.0 0.09171562755231338 0.0 52-53 2.0000842388420595E-4 0.0017294846065281338 0.0 0.12451700906932316 0.0 54-55 2.0000842388420595E-4 0.001741249807933087 0.0 0.16572462699017204 0.0 56-57 2.0000842388420595E-4 0.0017530150093380405 0.0 0.2165738274623802 0.0 58-59 2.0000842388420595E-4 0.001758897610040517 0.0 0.4594075844606161 0.0 60-61 2.294214273965892E-4 0.0017883106135529003 0.0 0.6817404780107209 0.0 62-63 2.4706922950401913E-4 0.0018294888184702367 0.0 0.6898172887752214 0.0 64-65 2.4706922950401913E-4 0.002023614641651966 0.0 0.6976411477095152 0.0 66-67 2.588344309089724E-4 0.002041262443759396 0.0 0.7053650024318672 0.0 68-69 2.7059963231392567E-4 0.0020824406486767326 0.0 0.7135594652104171 0.0 70-71 2.82364833718879E-4 0.0021000884507841626 0.0 0.7201715084000009 0.0 72-73 3.4119084074364544E-4 0.002105971051486639 0.0 0.7250658321844614 0.0 74-75 3.4119084074364544E-4 0.0021530318571064523 0.0 0.7287659880263192 0.0 76-77 3.470734414461221E-4 0.002200092662726265 0.0 0.7326543870906563 0.0 78-79 3.6472124355355203E-4 0.002217740464833695 0.0 0.7350662533786717 0.0 80-81 3.6472124355355203E-4 0.002223623065536172 0.0 0.735883934876316 0.0 82-83 3.6472124355355203E-4 0.0022353882669411255 0.0 0.7364133689395389 0.0 84-85 3.7060384425602864E-4 0.0022353882669411255 0.0 0.7366486729676379 0.0 86-87 3.8236904566098196E-4 0.0022706838711559854 0.0 0.7367663249816875 0.0 88 3.882516463634586E-4 0.002376570683800565 0.0 0.7367839727837949 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8855 0.0 47.706543 1 GTATCAA 15990 0.0 35.436398 1 TCAACGC 18920 0.0 29.369265 4 ATCAACG 19250 0.0 28.800087 3 CAACGCA 19335 0.0 28.717089 5 AACGCAG 19930 0.0 27.87894 6 TATCAAC 20440 0.0 27.494642 2 ACGCAGA 23140 0.0 24.011549 7 CGCAGAG 24260 0.0 22.885645 8 GTACATG 23260 0.0 22.711205 1 TACATGG 23835 0.0 22.02181 2 ACATGGG 24035 0.0 21.15446 3 CATGGGG 17860 0.0 19.309484 4 CGAGACC 10680 0.0 19.299356 86-87 GCAGAGT 28995 0.0 19.235561 9 ACCGAGG 11490 0.0 18.542553 90-91 AGACCGA 11350 0.0 18.52168 88-89 GACCGAG 11375 0.0 18.344624 90-91 GAGACCG 11250 0.0 18.252344 88-89 GAGTACT 16395 0.0 18.063547 12-13 >>END_MODULE