FastQCFastQC Report
Fri 27 May 2016
SRR2139571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7770243
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103750.051.7027631
ATCTCGT121250.049.45788294
TATCTCG146300.040.30325394
GTATCAA183900.035.41611
TCAACGC225050.028.6400434
ATCAACG224850.028.6091613
CAACGCA227400.028.3254955
AACGCAG236000.027.452786
TATCAAC239650.027.1419722
CTATCTC173800.023.99506892-93
GTACATG278600.023.8628181
TACATGG278400.023.6979052
ACGCAGA274550.023.5211757
GCCGTCT81200.023.24594590-91
CGCAGAG281050.022.9471328
ACATGGG279850.022.8959353
ATGCCGT81250.022.75584288-89
TGCCGTC80150.022.7462788-89
CCGTCTT81650.022.65332890-91
TATGCCG78950.022.63907286-87
Produced by FastQC (version 0.11.5)