FastQCFastQC Report
Fri 27 May 2016
SRR2139570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10336401
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102000.042.8559761
GTATCAA166950.030.5962681
TCAACGC197800.024.9549734
ATCAACG201000.024.5373173
CAACGCA203200.024.2513985
AACGCAG208700.023.6907336
TATCAAC215550.023.2989772
GTACATG256550.021.1549421
TACATGG258350.020.4688932
ACATGGG258200.020.1476973
ACGCAGA248800.019.888867
CGCAGAG257800.019.131028
CATGGGG192250.017.7769074
GTGGTAT57750.016.3010711
GCAGAGT309750.015.8563769
TGGTATC53200.015.6179742
AGAGTAC275450.015.52118510-11
CAGAGTA301900.015.13752410-11
ATGGGGG117950.015.129435
GAGTACT177800.015.12491112-13
Produced by FastQC (version 0.11.5)