##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139570_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10336401
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14139002540633	33.0	31.0	34.0	30.0	34.0
2	32.268526927312514	34.0	31.0	34.0	30.0	34.0
3	32.321222541579026	34.0	31.0	34.0	30.0	34.0
4	35.94970473765482	37.0	35.0	37.0	35.0	37.0
5	35.81200081150102	37.0	35.0	37.0	35.0	37.0
6	35.83474789726134	37.0	35.0	37.0	35.0	37.0
7	35.74848702173997	37.0	35.0	37.0	33.0	37.0
8	35.714355509233826	37.0	35.0	37.0	33.0	37.0
9	37.344225422368964	39.0	37.0	39.0	34.0	39.0
10-11	37.24281488305262	39.0	37.0	39.0	33.0	39.0
12-13	37.20032373937505	39.0	37.0	39.0	33.0	39.0
14-15	38.393596668705094	40.0	38.0	41.0	33.0	41.0
16-17	38.407868125472305	40.0	38.0	41.0	33.0	41.0
18-19	38.39881202364343	40.0	38.0	41.0	33.5	41.0
20-21	38.365290356759935	40.0	38.0	41.0	34.0	41.0
22-23	38.2608247009932	40.0	38.0	41.0	33.0	41.0
24-25	38.16999061809655	40.0	38.0	41.0	33.0	41.0
26-27	37.99297161310482	40.0	37.0	41.0	33.0	41.0
28-29	37.855033563725314	40.0	37.0	41.0	33.0	41.0
30-31	37.70374481431614	40.0	37.0	41.0	32.0	41.0
32-33	37.907169794781225	40.0	37.0	41.0	33.0	41.0
34-35	37.89074854605046	40.0	37.0	41.0	33.0	41.0
36-37	37.80461694207424	40.0	37.0	41.0	33.0	41.0
38-39	37.69491711531313	40.0	37.0	41.0	33.0	41.0
40-41	37.63638980762504	40.0	37.0	41.0	32.5	41.0
42-43	37.52357659358704	40.0	36.5	41.0	32.0	41.0
44-45	37.56400687471208	39.5	36.5	41.0	32.5	41.0
46-47	37.70193040778351	40.0	37.0	41.0	32.5	41.0
48-49	37.62232299671136	40.0	37.0	41.0	32.0	41.0
50-51	37.49792658413983	40.0	36.0	41.0	32.0	41.0
52-53	37.33859862584394	39.0	36.0	41.0	31.0	41.0
54-55	37.22204953413957	39.0	36.0	41.0	31.0	41.0
56-57	37.042653731538174	39.0	35.0	40.5	31.0	41.0
58-59	36.883608239974436	39.0	35.0	40.0	31.0	41.0
60-61	36.64258777697619	38.0	35.0	40.0	31.0	41.0
62-63	36.369927883118066	38.0	35.0	40.0	30.0	41.0
64-65	36.13774448556012	37.5	34.5	40.0	30.0	41.0
66-67	35.8371948879832	37.0	34.0	40.0	29.5	41.0
68-69	35.50913511523305	36.5	34.0	39.0	29.0	41.0
70-71	35.2020150594862	36.0	34.0	39.0	29.0	40.5
72-73	34.894815829707326	36.0	34.0	39.0	29.0	40.0
74-75	34.505257598431115	35.0	33.0	38.0	29.0	40.0
76-77	33.447392347886385	34.5	31.5	36.5	27.5	39.0
78-79	33.79264122326569	35.0	33.0	37.0	28.0	39.0
80-81	33.674493037986004	35.0	33.0	36.0	28.5	38.5
82-83	33.45466419081476	35.0	33.0	36.0	28.0	37.5
84-85	33.24034514553334	35.0	32.5	35.5	28.0	37.0
86-87	33.036979488386166	34.5	32.0	35.0	28.0	36.5
88-89	32.811233132228914	34.0	32.0	35.0	27.0	36.0
90-91	32.672564912118006	34.0	32.0	35.0	27.0	36.0
92-93	32.58596033183431	34.0	32.0	35.0	27.0	36.0
94-95	32.53710281853249	34.0	32.0	35.0	27.0	35.0
96-97	32.47799427288542	34.0	32.0	35.0	27.0	35.0
98-99	32.612721839090156	34.0	32.0	35.0	28.5	35.0
100-101	31.67447616745571	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	13.0
20	86.0
21	435.0
22	2178.0
23	7060.0
24	18582.0
25	38829.0
26	68817.0
27	106349.0
28	151233.0
29	199179.0
30	250661.0
31	308631.0
32	382500.0
33	483578.0
34	641557.0
35	882512.0
36	1331938.0
37	2083986.0
38	2220818.0
39	1116608.0
40	40848.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.59773812954819	21.301089228252657	13.851591090554633	25.249581551644525
2	15.028786476987799	22.386280863022538	38.99808412642192	23.586848533567746
3	17.778905829988602	26.262361531832983	30.95056973892557	25.008162899252845
4	12.596919848890947	18.143883310746435	38.07620775405229	31.182989086310325
5	12.97202962617259	37.91713382636761	35.998990364247675	13.111846183212124
6	30.863479464467368	37.98743876132515	18.003810030202967	13.145271744004514
7	26.491890165638893	32.347525990913084	23.43205338105594	17.728530462392083
8	24.829928715033407	36.60335933174419	21.125486520888654	17.44122543233375
9	25.601464184680918	15.673811416565592	21.07836180117238	37.64636259758111
10-11	24.404698501925377	26.524062872560766	29.16723625563675	19.904002369877098
12-13	24.717534662209797	24.175237589950314	29.204604194438666	21.902623553401227
14-15	21.90613057678393	25.71873904659852	26.39025904664496	25.984871329972588
16-17	21.880760182522444	28.595703539961708	27.29260343705967	22.230932840456173
18-19	21.911935305141508	28.010581245832082	28.63211769744614	21.445365751580265
20-21	22.523594860421518	27.306326704914458	28.492945185714813	21.67713324894921
22-23	22.268058724027195	27.274488773454465	28.328227304968674	22.12922519754967
24-25	22.126312976575782	27.373699387796613	28.404471866842627	22.095515768784978
26-27	22.109467722753678	27.479394708284648	28.3308533637175	22.080284205244176
28-29	22.063513475222994	27.499810494648923	28.381939134998284	22.054736895129796
30-31	22.266248181041192	27.466681786965115	28.24367143481527	22.023398597178417
32-33	22.06574453106667	27.596140487560373	28.231090525522728	22.10702445585023
34-35	22.1955040949475	27.59733881785306	28.268892522632367	21.938264564567074
36-37	22.18105446380339	27.493746146670272	28.218634436993856	22.106564952532487
38-39	22.02281384137204	27.56839509200701	28.3011989405542	22.10759212606675
40-41	22.229416053609114	27.468499742533343	28.189781701301648	22.1123025025559
42-43	22.096825492536343	27.57473277780488	28.0377994075858	22.29064232207298
44-45	22.148667535231453	27.64961018228322	27.923198899804326	22.278523382681005
46-47	22.207825106056955	27.57479819969416	27.773145127687094	22.44423156656179
48-49	22.159835687408936	27.708740513507728	27.717140792177158	22.41428300690618
50-51	22.164039339624164	27.782824690476954	27.63069905314576	22.42243691675312
52-53	22.23888895934438	27.67449626954224	27.54810981917143	22.538504951941952
54-55	22.24354505008846	27.594311336285944	27.572979496235483	22.58916411739012
56-57	22.222502467219815	27.662734470685468	27.536438202312354	22.578324859782363
58-59	22.198805962807295	27.703221697898417	27.543261883951693	22.554710455342594
60-61	22.247356051868728	27.65904161604408	27.526094375145444	22.56750795694175
62-63	22.21370715126337	27.789222667166886	27.472347600706044	22.5247225808637
64-65	22.179896919206705	27.745552714847037	27.43827715647355	22.63627320947271
66-67	22.256076870018436	27.880701254359185	27.208649278788734	22.654572596833646
68-69	22.252456542652457	27.837937111915178	27.24050475299684	22.669101592435524
70-71	22.209963230852058	27.61907410761958	27.32658609204285	22.844376569485515
72-73	22.266064713619983	27.64786602510501	27.241737204219053	22.844332057055954
74-75	22.255608412426252	27.696951973783346	27.222189674548364	22.825249939242042
76-77	22.47561584490971	27.69207250188492	27.139407013659667	22.692904639545702
78-79	22.3773105889002	27.621978140276703	27.14281228794037	22.857898982882723
80-81	22.395498206712897	27.64131701660203	27.13610950962227	22.827075267062803
82-83	22.434726611953966	27.614038515888723	27.24731440013583	22.70392047202148
84-85	22.462213162476985	27.667178641335404	27.226259032165245	22.644349164022366
86-87	22.431665080236584	27.616684466141628	27.29429912830493	22.657351325316853
88-89	22.380139060281103	27.57854307937249	27.340297464721488	22.701020395624923
90-91	22.412570364312277	27.647773387584774	27.32202678461298	22.61762946348997
92-93	22.48753870205859	27.520330664548016	27.406571541127217	22.585559092266177
94-95	22.32450985731176	27.48696981317141	27.573040564730185	22.615479764786645
96-97	22.20293680946749	27.553456677699312	27.663258298198816	22.58034821463438
98-99	22.323175881345854	28.109806590216017	27.7900399833775	21.77697754506062
100-101	25.265970265591875	35.812342032276504	28.970481722361523	9.9512059797701
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2269.0
1	2034.0
2	2609.5
3	3664.0
4	4885.5
5	7438.5
6	9622.5
7	11400.5
8	12538.0
9	12431.5
10	11589.0
11	10728.5
12	11849.5
13	15746.5
14	24350.0
15	35393.0
16	43527.0
17	47402.0
18	48349.5
19	47890.0
20	46750.5
21	46411.5
22	48390.0
23	54291.0
24	64773.5
25	79595.5
26	97647.5
27	117298.0
28	138727.0
29	161411.5
30	183053.5
31	204470.0
32	226372.5
33	247502.0
34	268536.5
35	290555.0
36	315045.0
37	336978.0
38	354935.0
39	372039.0
40	387850.0
41	401530.5
42	411370.5
43	420674.5
44	431256.0
45	440907.5
46	448013.5
47	451919.5
48	455444.0
49	454722.0
50	449017.0
51	440122.0
52	423980.5
53	402449.0
54	379247.0
55	354179.5
56	326292.5
57	296420.0
58	265498.0
59	231495.5
60	195740.5
61	161519.0
62	130135.0
63	102494.5
64	77660.5
65	58578.5
66	44916.0
67	34634.5
68	26837.0
69	20102.0
70	14978.5
71	11218.0
72	8056.5
73	5699.5
74	3977.0
75	2775.0
76	1932.5
77	1329.0
78	915.5
79	618.0
80	448.5
81	320.0
82	234.0
83	163.0
84	116.5
85	90.5
86	68.5
87	54.5
88	39.0
89	27.0
90	21.0
91	16.5
92	12.0
93	5.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001199643860566168
3	0.0
4	2.4186368156575968E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0034538133727590485
18-19	0.0
20-21	0.002143017333575596
22-23	0.0
24-25	4.01659016272223E-4
26-27	0.003417859847510198
28-29	0.0
30-31	1.406612611999104E-4
32-33	8.464112794129136E-4
34-35	7.767689226771971E-4
36-37	0.010415446490717418
38-39	0.011869535109544202
40-41	0.025209862474421797
42-43	0.001939191205767882
44-45	0.0011640634022448656
46-47	0.0017990327407415986
48-49	0.0017061957147795539
50-51	6.882550025020716E-5
52-53	1.498291760107008E-4
54-55	0.006082854315314729
56-57	0.0035780109235469884
58-59	0.0036781205778770574
60-61	0.001559967770392496
62-63	4.1489012231870156E-4
64-65	7.081889150931582E-4
66-67	6.891715037161529E-4
68-69	5.929202007675234E-6
70-71	0.0
72-73	1.1002379081103305E-4
74-75	3.165113351087344E-4
76-77	0.0014877391383065343
78-79	0.002127359366487759
80-81	0.0114237716560185
82-83	0.010386484058339786
84-85	0.013405454905839342
86-87	0.006582368784742715
88-89	0.08846245449287196
90-91	4.013583399647048E-4
92-93	1.4674807369306717E-5
94-95	3.0882033044076645E-4
96-97	0.014659371843188832
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	327.0
20-21	1009.0
22-23	1943.0
24-25	3839.0
26-27	4887.0
28-29	9878.0
30-31	26956.0
32-33	39818.0
34-35	58092.0
36-37	86396.0
38-39	87358.0
40-41	90348.0
42-43	102922.0
44-45	119949.0
46-47	120351.0
48-49	111829.0
50-51	103228.0
52-53	93679.0
54-55	106452.0
56-57	111748.0
58-59	116227.0
60-61	114794.0
62-63	110903.0
64-65	120264.0
66-67	128597.0
68-69	127353.0
70-71	126583.0
72-73	123100.0
74-75	125217.0
76-77	125810.0
78-79	134323.0
80-81	135726.0
82-83	130223.0
84-85	132185.0
86-87	140661.0
88-89	148036.0
90-91	160373.0
92-93	168762.0
94-95	211442.0
96-97	268855.0
98-99	847499.0
100-101	5358459.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.1073139288618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.02313422378135	79.28780727619845
2	6.411869231762561	11.170414120839027
3	1.4870906235733716	3.886094093648176
4	0.5110266544327552	1.7805663685475992
5	0.23559345933122475	1.0260956710775764
6	0.11737072940025499	0.6134309383156501
7	0.056818275563241434	0.3464501156468694
8	0.03910517711117092	0.27250775510932046
9	0.02653233364583406	0.2080044284537673
>10	0.08914201302887997	1.2307670368394943
>50	0.0018849455087763334	0.11448388176561448
>100	4.152542522089987E-4	0.05534488633959835
>500	1.7078608444005846E-5	0.008033427218880685
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9023641787891162E-5
2	0.0	1.9349094525260775E-5	0.0	0.0	2.9023641787891162E-5
3	0.0	1.9349094525260775E-5	0.0	9.674547262630387E-6	2.9023641787891162E-5
4	0.0	4.837273631315194E-5	0.0	3.869818905052155E-5	2.9023641787891162E-5
5	0.0	4.837273631315194E-5	0.0	3.869818905052155E-5	2.9023641787891162E-5
6	0.0	4.837273631315194E-5	0.0	4.837273631315194E-5	3.869818905052155E-5
7	0.0	4.837273631315194E-5	0.0	5.8047283575782324E-5	3.869818905052155E-5
8	0.0	5.8047283575782324E-5	0.0	5.8047283575782324E-5	3.869818905052155E-5
9	9.674547262630387E-6	1.2576911441419505E-4	0.0	7.73963781010431E-5	3.869818905052155E-5
10-11	9.674547262630387E-6	1.451182089394558E-4	0.0	9.674547262630388E-5	4.837273631315194E-5
12-13	1.4511820893945581E-5	1.6446730346471658E-4	0.0	1.2576911441419505E-4	4.837273631315194E-5
14-15	1.9349094525260775E-5	1.7414185072734697E-4	0.0	1.547927562020862E-4	6.288455720709752E-5
16-17	2.9023641787891162E-5	1.7414185072734697E-4	0.0	1.8865367162129254E-4	7.73963781010431E-5
18-19	3.869818905052155E-5	1.9349094525260773E-4	0.0	2.612127760910205E-4	8.223365173235829E-5
20-21	3.869818905052155E-5	2.225145870404989E-4	0.0	5.659610148638777E-4	1.1125729352024946E-4
22-23	4.353546268183674E-5	2.467009551970749E-4	0.0	0.0011561083978843313	1.3544366167682543E-4
24-25	4.837273631315194E-5	2.8056187061628124E-4	0.0	0.002795944158900182	1.547927562020862E-4
26-27	5.8047283575782324E-5	2.902364178789116E-4	0.0	0.006868928556467575	1.547927562020862E-4
28-29	5.8047283575782324E-5	2.902364178789116E-4	0.0	0.03194051778757422	1.6446730346471658E-4
30-31	5.8047283575782324E-5	3.2409733329811797E-4	0.0	0.054462863814977766	1.6446730346471658E-4
32-33	5.8047283575782324E-5	3.3860915419206355E-4	0.0	0.08088888966285267	1.6446730346471658E-4
34-35	5.8047283575782324E-5	3.8214461687390033E-4	0.0	0.12015787700186942	1.6446730346471658E-4
36-37	6.288455720709752E-5	4.2568007955573706E-4	0.0	0.17495934997103924	1.6446730346471658E-4
38-39	6.772183083841271E-5	4.450291740809978E-4	0.0	0.2520800034750974	1.6446730346471658E-4
40-41	6.772183083841271E-5	4.7889008950020417E-4	0.0	0.3495946026087804	1.6446730346471658E-4
42-43	8.707092536367348E-5	4.934019103941498E-4	0.0	0.40884636731876023	1.6930457709603177E-4
44-45	8.707092536367348E-5	5.46611920338617E-4	0.0	0.4944564360457765	1.7897912435866216E-4
46-47	1.0642001988893426E-4	5.949846566517688E-4	0.0	0.5886575027420086	1.8865367162129254E-4
48-49	1.0642001988893426E-4	5.99821930283084E-4	0.0	0.6627693720473886	1.9349094525260776E-4
50-51	1.0642001988893426E-4	6.385201193336056E-4	0.0	0.7313619121394381	2.1284003977786853E-4
52-53	1.0642001988893426E-4	6.627064874901816E-4	0.0	0.7981936846296888	2.273518606718141E-4
54-55	1.0642001988893426E-4	6.772183083841271E-4	0.0	0.9321474660280691	2.467009551970749E-4
56-57	1.0642001988893426E-4	6.772183083841271E-4	0.0	1.0445898915879908	2.515382288283901E-4
58-59	1.0642001988893426E-4	6.868928556467575E-4	0.0	1.1343551783643069	2.515382288283901E-4
60-61	1.0642001988893426E-4	7.062419501720182E-4	0.0	1.1815089217223673	2.515382288283901E-4
62-63	1.1609456715156465E-4	7.062419501720182E-4	0.0	1.2099956261371827	2.612127760910205E-4
64-65	1.1609456715156465E-4	7.546146864851703E-4	0.0	1.231182884642343	2.6605004972233567E-4
66-67	1.2576911441419505E-4	7.642892337478007E-4	0.0	1.243256719626106	2.7088732335365086E-4
68-69	1.451182089394558E-4	7.691265073791159E-4	0.0	1.2542228189482973	2.7572459698496605E-4
70-71	1.451182089394558E-4	7.73963781010431E-4	0.0	1.264056996240761	2.8056187061628124E-4
72-73	1.451182089394558E-4	7.73963781010431E-4	0.0	1.2714628621703046	3.144227860354876E-4
74-75	1.451182089394558E-4	7.73963781010431E-4	0.0	1.2779641579307923	3.192600596668028E-4
76-77	1.451182089394558E-4	7.73963781010431E-4	0.0	1.2840010754226738	3.192600596668028E-4
78-79	1.451182089394558E-4	7.73963781010431E-4	0.0	1.2894284964370093	3.192600596668028E-4
80-81	1.49955482570771E-4	7.73963781010431E-4	0.0	1.2925533752028389	3.2409733329811797E-4
82-83	1.547927562020862E-4	7.788010546417462E-4	0.0	1.2942996309837436	3.3860915419206355E-4
84-85	1.547927562020862E-4	7.836383282730614E-4	0.0	1.296113608595487	3.3860915419206355E-4
86-87	1.547927562020862E-4	7.933128755356918E-4	0.0	1.296403845013366	3.3860915419206355E-4
88-89	1.596300298334014E-4	7.933128755356918E-4	0.0	1.2964957532123609	3.3860915419206355E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10200	0.0	42.855976	1
GTATCAA	16695	0.0	30.596268	1
TCAACGC	19780	0.0	24.954973	4
ATCAACG	20100	0.0	24.537317	3
CAACGCA	20320	0.0	24.251398	5
AACGCAG	20870	0.0	23.690733	6
TATCAAC	21555	0.0	23.298977	2
GTACATG	25655	0.0	21.154942	1
TACATGG	25835	0.0	20.468893	2
ACATGGG	25820	0.0	20.147697	3
ACGCAGA	24880	0.0	19.88886	7
CGCAGAG	25780	0.0	19.13102	8
CATGGGG	19225	0.0	17.776907	4
GTGGTAT	5775	0.0	16.301071	1
GCAGAGT	30975	0.0	15.856376	9
TGGTATC	5320	0.0	15.617974	2
AGAGTAC	27545	0.0	15.521185	10-11
CAGAGTA	30190	0.0	15.137524	10-11
ATGGGGG	11795	0.0	15.12943	5
GAGTACT	17780	0.0	15.124911	12-13
>>END_MODULE