FastQCFastQC Report
Fri 27 May 2016
SRR2139569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10952427
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109700.045.042661
GTATCAA181150.031.3101331
TCAACGC218750.025.3807724
CAACGCA222850.024.9508635
ATCAACG223100.024.8858993
AACGCAG227750.024.3777986
TATCAAC234600.023.9478572
GTACATG273050.020.273241
ACGCAGA273750.020.23627
TACATGG278050.019.4929852
CGCAGAG284800.019.4220648
ACATGGG279050.018.9199123
AGAGTAC298050.016.30210710-11
GAGTACT192550.016.28323212-13
GCAGAGT342200.016.0798039
GTGGTAT60350.016.0741181
CATGGGG207600.016.046374
GAGTACA205250.015.6470681
CAGAGTA330350.015.63914110-11
TGGTATC58150.014.9785332
Produced by FastQC (version 0.11.5)