##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139569_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10952427
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15587997071334	33.0	31.0	34.0	30.0	34.0
2	32.28293564522274	34.0	31.0	34.0	30.0	34.0
3	32.33615992144937	34.0	31.0	34.0	30.0	34.0
4	35.963978029709764	37.0	35.0	37.0	35.0	37.0
5	35.826912062504505	37.0	35.0	37.0	35.0	37.0
6	35.84787663957952	37.0	35.0	37.0	35.0	37.0
7	35.761809231871624	37.0	35.0	37.0	33.0	37.0
8	35.72726702492516	37.0	35.0	37.0	33.0	37.0
9	37.36006795571429	39.0	37.0	39.0	34.0	39.0
10-11	37.25887385508253	39.0	37.0	39.0	33.5	39.0
12-13	37.217967761848584	39.0	37.0	39.0	33.0	39.0
14-15	38.41778356523171	40.0	38.0	41.0	33.0	41.0
16-17	38.43325506757543	40.0	38.0	41.0	33.0	41.0
18-19	38.426406402891345	40.0	38.0	41.0	33.5	41.0
20-21	38.39106434175214	40.0	38.0	41.0	34.0	41.0
22-23	38.28778855652962	40.0	38.0	41.0	33.5	41.0
24-25	38.19785094781464	40.0	38.0	41.0	33.0	41.0
26-27	38.02634310091324	40.0	37.0	41.0	33.0	41.0
28-29	37.89016310400736	40.0	37.0	41.0	33.0	41.0
30-31	37.737189546571926	40.0	37.0	41.0	32.5	41.0
32-33	37.931640766032245	40.0	37.0	41.0	33.0	41.0
34-35	37.91660169709425	40.0	37.0	41.0	33.0	41.0
36-37	37.832946466991075	40.0	37.0	41.0	33.0	41.0
38-39	37.72284335702166	40.0	37.0	41.0	33.0	41.0
40-41	37.66796089638616	40.0	37.0	41.0	33.0	41.0
42-43	37.55378318044548	40.0	37.0	41.0	32.0	41.0
44-45	37.59503589142735	39.5	36.5	41.0	32.5	41.0
46-47	37.73513302466096	40.0	37.0	41.0	33.0	41.0
48-49	37.656280949498374	40.0	37.0	41.0	32.0	41.0
50-51	37.529882500721826	40.0	36.0	41.0	32.0	41.0
52-53	37.37218233912948	39.0	36.0	41.0	31.0	41.0
54-55	37.2605910999699	39.0	36.0	41.0	31.0	41.0
56-57	37.08424934235287	39.0	35.0	41.0	31.0	41.0
58-59	36.92734938845243	39.0	35.0	40.0	31.0	41.0
60-61	36.68574704900097	38.0	35.0	40.0	31.0	41.0
62-63	36.40994306118587	38.0	35.0	40.0	30.0	41.0
64-65	36.17789381603036	37.5	34.5	40.0	30.0	41.0
66-67	35.87636591624331	37.0	34.0	40.0	30.0	41.0
68-69	35.549826209157764	36.5	34.0	39.0	29.0	41.0
70-71	35.241565426723035	36.0	34.0	39.0	29.0	40.5
72-73	34.93257838793866	36.0	34.0	39.0	29.0	40.0
74-75	34.54302047651902	35.0	33.0	38.0	29.0	40.0
76-77	33.48678980725478	34.5	32.0	36.5	27.5	39.0
78-79	33.82959777317076	35.0	33.0	37.0	28.5	39.0
80-81	33.70889363839554	35.0	33.0	36.0	29.0	38.5
82-83	33.487246374181225	35.0	33.0	36.0	29.0	37.5
84-85	33.26987289557811	35.0	32.5	35.5	29.0	37.0
86-87	33.062623971529334	34.5	32.0	35.0	28.0	36.5
88-89	32.83517951568504	34.0	32.0	35.0	27.5	36.0
90-91	32.695432626526994	34.0	32.0	35.0	27.0	36.0
92-93	32.610067371008284	34.0	32.0	35.0	27.0	36.0
94-95	32.55798939529328	34.0	32.0	35.0	27.0	35.0
96-97	32.4964563542057	34.0	32.0	35.0	27.0	35.0
98-99	32.62527604026544	34.0	32.0	35.0	28.5	35.0
100-101	31.682232982938718	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	14.0
20	80.0
21	485.0
22	2292.0
23	7801.0
24	19627.0
25	41315.0
26	72216.0
27	111966.0
28	157987.0
29	207818.0
30	262032.0
31	323622.0
32	401722.0
33	508153.0
34	675819.0
35	934207.0
36	1418717.0
37	2231581.0
38	2374537.0
39	1157543.0
40	42890.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.80802611147283	21.137853737806243	13.793700702136613	25.26041944858432
2	15.08018008665408	22.385287158850808	38.885582120184445	23.648950634310673
3	17.778525252896003	26.196878554862774	30.987259718781967	25.03733647345926
4	12.601038513718887	18.118330960485952	38.02445549977571	31.25617502601945
5	12.986829311895892	37.951095223004	35.933149794105	13.128925670995114
6	30.917521751115075	37.942393955239325	18.046000215294747	13.094084078350853
7	26.588362561101754	32.36251654541957	23.37991387662296	17.669207016855715
8	24.934911686697387	36.48551138482822	21.13635635279742	17.44322057567697
9	25.698486737231846	15.62549560932933	20.984673077483194	37.69134457595563
10-11	24.41034758779949	26.509585501003567	29.147306802410096	19.932760108786848
12-13	24.769478034411915	24.1498665090395	29.190639663701933	21.890015792846647
14-15	21.96458830540482	25.669584467442697	26.34947943501472	26.01634779213776
16-17	21.896298087267553	28.58969520284984	27.257296793035497	22.256709916847107
18-19	21.953325961451284	27.995621427104695	28.610804710225413	21.440247901218605
20-21	22.563441291042047	27.2943773332321	28.45559638084767	21.68658499487818
22-23	22.340548763407337	27.207636643054578	28.33075000715709	22.121064586380996
24-25	22.15889564374084	27.31453113200718	28.401477973684468	22.125095250567508
26-27	22.13778358632257	27.454429613161423	28.3191915675281	22.088595232987903
28-29	22.111254963107175	27.46859502288752	28.3486209345305	22.071529079474807
30-31	22.284873935426848	27.37346128653489	28.28872600511339	22.05293877292487
32-33	22.078544476582344	27.551053038086735	28.2374552655804	22.132947219750516
34-35	22.24140683445441	27.541062593504073	28.269493591775202	21.948036980266316
36-37	22.234287940059033	27.460200458659877	28.18716446065323	22.118347140627865
38-39	22.11382138857208	27.605907889759155	28.239506633199724	22.04076408846904
40-41	22.287445769700405	27.472905893848043	28.130295298553147	22.10935303789841
42-43	22.17155548720203	27.582879097920564	27.982055050231462	22.26351036464594
44-45	22.24192201820914	27.635423527656926	27.85753705144237	22.265117402691555
46-47	22.31607405983355	27.57672724362975	27.675093424379693	22.43210527215701
48-49	22.25377633617495	27.67279082227146	27.65185200281719	22.421580838736404
50-51	22.26218084522985	27.793240369620015	27.557824616427506	22.38675416872263
52-53	22.374608209266896	27.682120357993174	27.466719857409327	22.476551575330607
54-55	22.328867805641632	27.593541865635657	27.521215837116692	22.556374491606018
56-57	22.361799545731927	27.678200282599374	27.479979138165916	22.48002103350278
58-59	22.286949463282543	27.693177473100544	27.51564580530001	22.5042272583169
60-61	22.359973147577918	27.67765268390796	27.52417215060588	22.438202017908242
62-63	22.340940530349535	27.767955156163087	27.480260803050605	22.410843510436774
64-65	22.26737069541983	27.725840409924828	27.48969841542051	22.51709047923483
66-67	22.335980002828844	27.769911419759364	27.334930329640866	22.559178247770927
68-69	22.276264897221886	27.77949809173829	27.36426056478811	22.57997644625171
70-71	22.230577849458562	27.604743036104594	27.470018807997015	22.694660306439832
72-73	22.305388898474536	27.602814189032237	27.351884832614754	22.739912079878476
74-75	22.26030841864733	27.62900189181467	27.35809790459377	22.752591784944233
76-77	22.38518870929582	27.58437421309911	27.327655023352847	22.702782054252218
78-79	22.306281512693747	27.552602161030148	27.29436615287284	22.84675017340326
80-81	22.34689576508927	27.601384327186306	27.26523517253761	22.786484735186814
82-83	22.36074993631153	27.55667042849108	27.373770689521493	22.708808945675894
84-85	22.38020909320155	27.57084326697221	27.348540054728975	22.70040758509726
86-87	22.338101916723975	27.584427051695453	27.361732476642587	22.715738554937985
88-89	22.32309566154143	27.491383089045325	27.435901252862415	22.74961999655083
90-91	22.322790398706267	27.500716596616392	27.48976039476283	22.686732609914515
92-93	22.31974632271096	27.363896867585012	27.607101552361662	22.709255257342363
94-95	22.148584213144996	27.414945812813375	27.67077558074813	22.765694393293504
96-97	22.091442914879178	27.474130720369832	27.726776657893048	22.70764970685794
98-99	22.257911281935915	28.068035952303923	27.803676302712788	21.87037646304737
100-101	25.26709599346181	35.81800646218114	28.94061541634756	9.974282128009495
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2351.0
1	2130.0
2	2692.0
3	3734.0
4	5013.5
5	7612.5
6	9817.5
7	11552.0
8	12808.0
9	12851.5
10	12114.0
11	11380.0
12	12582.5
13	16645.0
14	25565.0
15	37285.0
16	46055.5
17	50119.5
18	51152.0
19	50718.0
20	49204.0
21	48235.0
22	49713.5
23	55402.0
24	65737.0
25	80567.0
26	99416.0
27	120104.0
28	142577.5
29	166273.0
30	188712.5
31	211668.5
32	236262.0
33	260439.0
34	283469.5
35	306496.0
36	332173.5
37	354446.5
38	372966.0
39	390713.0
40	406419.5
41	420333.0
42	430291.5
43	440321.5
44	450114.5
45	458715.5
46	466909.5
47	471118.0
48	472667.5
49	472498.5
50	468292.5
51	458416.5
52	442087.0
53	421133.0
54	396810.5
55	370657.0
56	343065.5
57	312872.0
58	280793.5
59	244399.5
60	206093.5
61	170245.0
62	137670.0
63	108240.0
64	82454.5
65	62844.5
66	48121.5
67	37504.5
68	29314.5
69	21939.5
70	16261.0
71	12080.0
72	8758.0
73	6232.5
74	4348.0
75	2984.0
76	2050.5
77	1404.5
78	974.5
79	676.5
80	460.0
81	308.0
82	229.0
83	168.0
84	127.5
85	103.0
86	77.5
87	56.5
88	39.5
89	26.5
90	18.0
91	13.0
92	10.5
93	5.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013147770809154903
3	0.0
4	4.017374413908442E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0034010726572293063
18-19	0.0
20-21	0.002232484243637534
22-23	0.0
24-25	3.242561996643628E-4
26-27	0.003289471429912671
28-29	0.0
30-31	1.0983933434434338E-4
32-33	8.992108094681943E-4
34-35	7.505364263415263E-4
36-37	0.010654711410589329
38-39	0.012250923927733918
40-41	0.025424604529397057
42-43	0.0019532207424836794
44-45	0.0012737187622269218
46-47	0.0017340201926167068
48-49	0.0017391557393388651
50-51	5.941962769939036E-5
52-53	1.2504076954290947E-4
54-55	0.006198556590068437
56-57	0.0036325003020697337
58-59	0.004099022881851894
60-61	0.001663859731810926
62-63	5.137561928145E-4
64-65	8.687135703355551E-4
66-67	7.120984383137661E-4
68-69	4.96332543686639E-5
70-71	0.0
72-73	2.2717739021567426E-4
74-75	3.4584103958433136E-4
76-77	0.0017676605679645585
78-79	0.001914937182031966
80-81	0.011135175159450447
82-83	0.010053767350901216
84-85	0.013073109408721223
86-87	0.006612360795631358
88-89	0.08985371931399688
90-91	4.111874829224553E-4
92-93	4.072300712801942E-5
94-95	3.20369466784024E-4
96-97	0.015157179009814056
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	321.0
20-21	1042.0
22-23	1930.0
24-25	3921.0
26-27	5072.0
28-29	9675.0
30-31	24125.0
32-33	35770.0
34-35	52659.0
36-37	79138.0
38-39	82536.0
40-41	85943.0
42-43	100161.0
44-45	116959.0
46-47	118676.0
48-49	110717.0
50-51	102926.0
52-53	96372.0
54-55	109294.0
56-57	113069.0
58-59	116227.0
60-61	117848.0
62-63	113814.0
64-65	123286.0
66-67	131196.0
68-69	133403.0
70-71	130876.0
72-73	128367.0
74-75	132305.0
76-77	132268.0
78-79	140338.0
80-81	141698.0
82-83	136476.0
84-85	138807.0
86-87	148165.0
88-89	156462.0
90-91	170652.0
92-93	179688.0
94-95	225689.0
96-97	289807.0
98-99	916045.0
100-101	5798704.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.98177899441322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.19142584857852	79.31992449346477
2	6.289488050190034	10.941417191392649
3	1.4003737075154834	3.654209890100965
4	0.5028554842458048	1.7495705838718894
5	0.2408827228312226	1.047620388043895
6	0.12116551530437311	0.6323515244369505
7	0.07218949474538658	0.43954194743630987
8	0.04527538248278948	0.3150506650404418
9	0.03097540338195593	0.24248661221088819
>10	0.10199841527346282	1.4123073746719208
>50	0.0026669054703492225	0.15329989728000756
>100	6.862664937915306E-4	0.08488221420412581
>500	1.6803486859235647E-5	0.007337217845234319
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.826079279049292E-5	0.0
2	0.0	0.0	0.0	1.826079279049292E-5	0.0
3	0.0	0.0	0.0	1.826079279049292E-5	0.0
4	0.0	9.13039639524646E-6	0.0	2.739118918573938E-5	0.0
5	0.0	9.13039639524646E-6	0.0	2.739118918573938E-5	0.0
6	9.13039639524646E-6	9.13039639524646E-6	0.0	2.739118918573938E-5	0.0
7	9.13039639524646E-6	9.13039639524646E-6	0.0	2.739118918573938E-5	9.13039639524646E-6
8	9.13039639524646E-6	9.13039639524646E-6	0.0	2.739118918573938E-5	1.826079279049292E-5
9	9.13039639524646E-6	2.739118918573938E-5	0.0	7.304317116197168E-5	1.826079279049292E-5
10-11	1.826079279049292E-5	2.739118918573938E-5	0.0	1.1869515313820398E-4	1.826079279049292E-5
12-13	2.739118918573938E-5	2.739118918573938E-5	0.0	1.2326035133582722E-4	1.826079279049292E-5
14-15	3.195638738336261E-5	3.652158558098584E-5	0.0	1.5521673871918984E-4	1.826079279049292E-5
16-17	4.56519819762323E-5	4.56519819762323E-5	0.0	2.0543391889304535E-4	1.826079279049292E-5
18-19	4.56519819762323E-5	5.934757656910199E-5	0.0	2.602162972645241E-4	1.826079279049292E-5
20-21	4.56519819762323E-5	7.760836935959492E-5	0.0	3.7891145040272814E-4	1.826079279049292E-5
22-23	4.56519819762323E-5	9.586916215008783E-5	0.0	8.080400809793117E-4	2.282599098811615E-5
24-25	4.56519819762323E-5	1.1869515313820398E-4	0.0	0.0015978193691681304	2.739118918573938E-5
26-27	4.56519819762323E-5	1.2782554953345043E-4	0.0	0.004346068684137314	2.739118918573938E-5
28-29	4.56519819762323E-5	1.2782554953345043E-4	0.0	0.02208186368190356	2.739118918573938E-5
30-31	5.478237837147876E-5	1.6434713511443629E-4	0.0	0.03308855653637317	2.739118918573938E-5
32-33	5.478237837147876E-5	1.826079279049292E-4	0.0	0.039466138418452826	2.739118918573938E-5
34-35	5.934757656910199E-5	2.1912951348591504E-4	0.0	0.048906968291137665	2.739118918573938E-5
36-37	6.391277476672522E-5	2.602162972645241E-4	0.0	0.06233321619034758	2.739118918573938E-5
38-39	6.391277476672522E-5	2.6478149546214734E-4	0.0	0.08456573141277271	2.739118918573938E-5
40-41	7.304317116197168E-5	2.921726846478867E-4	0.0	0.11869058794000636	4.56519819762323E-5
42-43	7.304317116197168E-5	3.1043347743837967E-4	0.0	0.13624377500986767	4.56519819762323E-5
44-45	7.304317116197168E-5	3.606506576122352E-4	0.0	0.1624617082588179	5.021718017385553E-5
46-47	7.304317116197168E-5	3.834766486003513E-4	0.0	0.19352788199364396	5.478237837147876E-5
48-49	7.304317116197168E-5	3.834766486003513E-4	0.0	0.21840364697249293	5.478237837147876E-5
50-51	7.304317116197168E-5	4.017374413908442E-4	0.0	0.24468092779801226	5.478237837147876E-5
52-53	7.304317116197168E-5	4.108678377860907E-4	0.0	0.27261994076746643	5.934757656910199E-5
54-55	7.304317116197168E-5	4.108678377860907E-4	0.0	0.35146547883861723	6.391277476672522E-5
56-57	7.304317116197168E-5	4.108678377860907E-4	0.0	0.41189500738055596	6.391277476672522E-5
58-59	7.304317116197168E-5	4.108678377860907E-4	0.0	0.4785971182460289	6.391277476672522E-5
60-61	7.760836935959492E-5	4.2912863057658365E-4	0.0	0.5155021804756151	6.391277476672522E-5
62-63	9.13039639524646E-5	4.382590269718301E-4	0.0	0.5340505807525583	7.304317116197168E-5
64-65	9.13039639524646E-5	4.930414053433089E-4	0.0	0.5529824576781018	7.304317116197168E-5
66-67	9.13039639524646E-5	5.021718017385553E-4	0.0	0.5634504571452519	7.304317116197168E-5
68-69	9.13039639524646E-5	5.021718017385553E-4	0.0	0.573032808162063	7.304317116197168E-5
70-71	9.13039639524646E-5	5.021718017385553E-4	0.0	0.5817432063231283	8.217356755721814E-5
72-73	9.13039639524646E-5	5.021718017385553E-4	0.0	0.589125131808685	8.217356755721814E-5
74-75	9.13039639524646E-5	5.113021981338017E-4	0.0	0.5955027136907646	8.217356755721814E-5
76-77	1.0043436034771106E-4	5.432585855171644E-4	0.0	0.6014557321404653	8.217356755721814E-5
78-79	1.0956475674295752E-4	5.569541801100341E-4	0.0	0.6070298391397633	8.217356755721814E-5
80-81	1.0956475674295752E-4	5.569541801100341E-4	0.0	0.6099606963826374	8.217356755721814E-5
82-83	1.0956475674295752E-4	5.569541801100341E-4	0.0	0.611795906058082	9.13039639524646E-5
84-85	1.1412995494058074E-4	5.569541801100341E-4	0.0	0.6132887258687048	9.13039639524646E-5
86-87	1.1869515313820398E-4	5.569541801100341E-4	0.0	0.6135443769677716	9.13039639524646E-5
88-89	1.1869515313820398E-4	5.75214972900527E-4	0.0	0.6135900289497479	1.0043436034771106E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10970	0.0	45.04266	1
GTATCAA	18115	0.0	31.310133	1
TCAACGC	21875	0.0	25.380772	4
CAACGCA	22285	0.0	24.950863	5
ATCAACG	22310	0.0	24.885899	3
AACGCAG	22775	0.0	24.377798	6
TATCAAC	23460	0.0	23.947857	2
GTACATG	27305	0.0	20.27324	1
ACGCAGA	27375	0.0	20.2362	7
TACATGG	27805	0.0	19.492985	2
CGCAGAG	28480	0.0	19.422064	8
ACATGGG	27905	0.0	18.919912	3
AGAGTAC	29805	0.0	16.302107	10-11
GAGTACT	19255	0.0	16.283232	12-13
GCAGAGT	34220	0.0	16.079803	9
GTGGTAT	6035	0.0	16.074118	1
CATGGGG	20760	0.0	16.04637	4
GAGTACA	20525	0.0	15.647068	1
CAGAGTA	33035	0.0	15.639141	10-11
TGGTATC	5815	0.0	14.978533	2
>>END_MODULE