FastQCFastQC Report
Fri 27 May 2016
SRR2139568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5860165
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66450.047.935381
GTATCAA117350.034.2679521
TCAACGC141050.028.154434
ATCAACG142650.027.8093383
CAACGCA143050.027.673135
AACGCAG148300.026.6645986
TATCAAC151750.026.4171562
GTACATG173100.023.2313371
TACATGG175900.022.9090652
ACGCAGA173550.022.7610477
ACATGGG176400.022.4886723
CGCAGAG179950.022.0212298
GTGGTAT33200.019.8936841
CATGGGG120250.019.5712914
TGGTATC32750.018.7629972
GCAGAGT214550.018.4893979
AGAGTAC189800.017.62991310-11
GAGTACT125400.017.35035112-13
CAGAGTA208150.017.04968510-11
ATGGGGG71450.016.4984475
Produced by FastQC (version 0.11.5)