##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139568_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5860165
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.55680275214094	25.0	18.0	31.0	18.0	33.0
2	31.47121966702303	32.0	31.0	33.0	27.0	33.0
3	31.917732009252298	33.0	31.0	33.0	29.0	34.0
4	32.62530167665928	33.0	33.0	34.0	31.0	34.0
5	33.0046689811635	34.0	33.0	34.0	32.0	34.0
6	37.097117060697094	38.0	38.0	38.0	36.0	38.0
7	37.18001165496193	38.0	38.0	38.0	36.0	38.0
8	37.26974667095551	38.0	38.0	38.0	36.0	38.0
9	37.357953061048626	38.0	38.0	38.0	37.0	38.0
10-11	37.37947856417012	38.0	38.0	38.0	37.0	38.0
12-13	37.40318378407434	38.0	38.0	38.0	37.0	38.0
14-15	37.402560593430394	38.0	38.0	38.0	37.0	38.0
16-17	37.39308722535969	38.0	38.0	38.0	37.0	38.0
18-19	37.2790365970924	38.0	38.0	38.0	37.0	38.0
20-21	37.389416802095425	38.0	38.0	38.0	37.0	38.0
22-23	37.40784289278503	38.0	38.0	38.0	37.0	38.0
24-25	37.41649070185622	38.0	38.0	38.0	37.0	38.0
26-27	37.38956857396103	38.0	38.0	38.0	37.0	38.0
28-29	37.36508691047855	38.0	38.0	38.0	37.0	38.0
30-31	37.36964865287656	38.0	38.0	38.0	37.0	38.0
32-33	37.355133246166034	38.0	38.0	38.0	37.0	38.0
34-35	37.338036864668666	38.0	38.0	38.0	37.0	38.0
36-37	37.31538683871834	38.0	38.0	38.0	37.0	38.0
38-39	37.28277904309302	38.0	38.0	38.0	37.0	38.0
40-41	37.286106179323916	38.0	38.0	38.0	37.0	38.0
42-43	37.2731420021437	38.0	38.0	38.0	37.0	38.0
44-45	37.244206209508306	38.0	38.0	38.0	37.0	38.0
46-47	37.215061857443985	38.0	38.0	38.0	37.0	38.0
48-49	37.18610392996471	38.0	38.0	38.0	37.0	38.0
50-51	37.16134476319665	38.0	38.0	38.0	37.0	38.0
52-53	37.14332259444372	38.0	38.0	38.0	36.5	38.0
54-55	37.13101340158297	38.0	38.0	38.0	36.0	38.0
56-57	37.11887485367933	38.0	38.0	38.0	36.0	38.0
58-59	37.10983653900084	38.0	38.0	38.0	36.0	38.0
60-61	37.10620122672337	38.0	38.0	38.0	36.0	38.0
62-63	37.10036489002283	38.0	38.0	38.0	36.0	38.0
64-65	37.10711179353223	38.0	38.0	38.0	36.0	38.0
66-67	37.0973181776738	38.0	38.0	38.0	36.0	38.0
68-69	37.09852330802529	38.0	38.0	38.0	36.0	38.0
70-71	37.09031033670075	38.0	38.0	38.0	36.0	38.0
72-73	37.063878184407194	38.0	38.0	38.0	36.0	38.0
74-75	37.083239736711995	38.0	38.0	38.0	36.0	38.0
76-77	37.072505432164945	38.0	38.0	38.0	36.0	38.0
78-79	37.062542479528894	38.0	38.0	38.0	36.0	38.0
80-81	37.03987033163635	38.0	38.0	38.0	36.0	38.0
82-83	37.03027766494455	38.0	38.0	38.0	36.0	38.0
84-85	37.01763311998798	38.0	38.0	38.0	36.0	38.0
86-87	36.98381575344551	38.0	38.0	38.0	36.0	38.0
88-89	36.96908579255463	38.0	38.0	38.0	36.0	38.0
90-91	36.9758812149633	38.0	38.0	38.0	36.0	38.0
92-93	36.94499152839499	38.0	38.0	38.0	35.5	38.0
94-95	36.8852232632903	38.0	38.0	38.0	35.0	38.0
96-97	36.89860057070588	38.0	38.0	38.0	35.0	38.0
98-99	36.92377598203305	38.0	38.0	38.0	35.0	38.0
100	35.414022668091754	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	20.0
20	55.0
21	158.0
22	418.0
23	1116.0
24	2597.0
25	4991.0
26	9098.0
27	14965.0
28	23027.0
29	33487.0
30	46154.0
31	61359.0
32	81001.0
33	109105.0
34	159521.0
35	279612.0
36	842602.0
37	4190875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57941542601616	19.180483825967357	14.085627281825683	24.154473466190797
2	15.402774495257384	21.225699276385562	39.89162421194625	23.4799020164108
3	18.560450089715903	25.700214925688954	30.550112496832426	25.18922248776272
4	12.574884836860395	17.145745896233297	38.4295322742619	31.849836992644402
5	13.205001901899527	37.75399403167034	35.66995925378644	13.371044812643698
6	30.729612474215685	37.43286863548932	18.374967065700684	13.46255182459431
7	26.75455383935435	32.36100007422999	23.199773385220382	17.68467270119527
8	25.048629176823518	36.08898384260512	21.255220629453266	17.6071663511181
9	25.960514763662797	15.710410884335168	21.07566937108426	37.25340498091777
10-11	24.530956039633697	26.306255881873636	29.04969399325787	20.1130940852348
12-13	24.998929211037574	24.020774159089378	29.079573698010208	21.90072293186284
14-15	22.197981336186267	25.367671055594265	26.48266554337605	25.95168206484341
16-17	21.933861930508783	28.344184848037557	27.350467094356556	22.371486127097103
18-19	21.987264863702645	27.521196075537123	28.877156189288183	21.61438287147205
20-21	22.625076822907445	26.822886114727158	28.869526013219808	21.682511049145585
22-23	22.396517369804965	26.790625452232014	28.70288425044848	22.109972927514544
24-25	22.222641299483875	26.89544758077316	28.81566717877529	22.066243940967677
26-27	22.164929185636094	27.020932212893182	28.77737627136349	22.03676233010723
28-29	22.09950840862018	26.94391179699786	28.866607814578355	22.0899719798036
30-31	22.32632832936835	26.933128272137196	28.70804608782862	22.032497310665832
32-33	22.134625654454297	27.034558544994265	28.72536742729195	22.10544837325949
34-35	22.2277492230395	27.140513723981407	28.762228596753804	21.869508456225294
36-37	22.259824881403027	26.964118838609718	28.640273234151124	22.135783045836128
38-39	22.10549654106991	27.07705049353992	28.773676107147388	22.043776858242776
40-41	22.272580103149732	26.956260476268167	28.708177010323478	22.06298241025862
42-43	22.173733767644784	27.039129723516876	28.540652392899936	22.246484115938404
44-45	22.190287854073148	27.184414618105073	28.427362300714854	22.197935227106925
46-47	22.31536065819736	27.097902318327215	28.2181422509916	22.368594772483828
48-49	22.27101474701209	27.18512639568116	28.203232942807098	22.34062591449965
50-51	22.235492415652597	27.321090370078515	28.124493485558112	22.318923728710775
52-53	22.338183123494343	27.28033625673376	28.06487595446658	22.316604665305317
54-55	22.292555387367553	27.23601894314902	28.029831996098288	22.44159367338514
56-57	22.333987428731632	27.3811093490745	27.953838508367383	22.331064713826486
58-59	22.277717903458292	27.392751326904257	28.066306349991056	22.263224419646395
60-61	22.34139883900246	27.441316393816038	27.997287455758556	22.219997311422944
62-63	22.2917155808149	27.56616155361622	27.947848174650954	22.194274690917926
64-65	22.2495611634434	27.477277513862852	27.92977312352344	22.34338819917031
66-67	22.322451009978543	27.48660228469193	27.841132740479296	22.34981396485023
68-69	22.248516847394917	27.452841714227223	27.942585985159024	22.356055453218836
70-71	22.286150668023662	27.214000674132606	28.001747341893495	22.498101315950237
72-73	22.380615180762423	27.199512844669805	27.928339003236335	22.491532971331434
74-75	22.25566660203028	27.325038912850935	27.939322156479445	22.479972328639334
76-77	22.40413375397105	27.27966681556122	27.804488301172537	22.511711129295193
78-79	22.308783246002246	27.25819749620697	27.808789741747425	22.62422951604336
80-81	22.378831066770726	27.34797549126376	27.753924406640067	22.51926903532545
82-83	22.44402774938093	27.337350473395173	27.820241845352108	22.39837993187179
84-85	22.468515908162132	27.33235631894491	27.72623334882712	22.47289442406584
86-87	22.33990677363094	27.395085946828974	27.7906717031711	22.47433557636899
88-89	22.441399253565223	27.35962909766871	27.6883375673313	22.51063408143477
90-91	22.44128312825743	27.42649122622387	27.682615892450542	22.44960975306816
92-93	22.550159664444536	27.36653969441355	27.668924888577045	22.414375752564872
94-95	22.40397218030017	27.397503103933722	27.71855261287876	22.479972102887345
96-97	22.505904766048005	27.527164340711497	27.73395350748239	22.232977385758108
98-99	23.596728677050056	29.389573230349665	27.959477974814344	19.05422011778594
100	30.03664987699902	40.594997527366836	29.36835259563414	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1421.0
1	1353.5
2	1833.0
3	2627.5
4	3255.5
5	4247.5
6	5200.0
7	6480.5
8	7842.0
9	8475.0
10	8147.0
11	7730.0
12	8529.5
13	10407.5
14	13648.0
15	17130.5
16	19399.5
17	20310.0
18	20085.5
19	19137.0
20	18350.0
21	18570.0
22	20347.0
23	24224.5
24	30147.5
25	37908.0
26	47827.5
27	59206.0
28	70938.0
29	83929.0
30	96573.5
31	107921.5
32	121393.0
33	136178.5
34	148453.5
35	160751.5
36	174386.0
37	186069.5
38	196770.5
39	205566.0
40	213358.5
41	220987.0
42	227032.0
43	235682.0
44	243508.5
45	247854.0
46	249799.0
47	250853.0
48	252351.5
49	249995.0
50	245529.5
51	240854.5
52	233225.5
53	223474.0
54	211290.0
55	197893.0
56	183744.5
57	167781.5
58	151165.0
59	131709.5
60	110539.5
61	90152.5
62	72517.5
63	56942.0
64	43312.0
65	33055.5
66	25059.0
67	19217.0
68	14545.0
69	10830.5
70	8202.0
71	6044.5
72	4311.0
73	2992.5
74	2008.5
75	1352.0
76	885.5
77	556.5
78	367.0
79	235.5
80	161.0
81	109.0
82	75.0
83	48.5
84	35.5
85	27.5
86	16.0
87	9.5
88	5.0
89	1.0
90	1.0
91	2.0
92	3.0
93	3.0
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0039930616288107925
6	2.2183675715615515E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.5596548902633287E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	6.853144145436055E-5
34-35	1.2893453400609654E-4
36-37	1.0367046132232191E-4
38-39	1.3052220978974002E-4
40-41	7.01376319518996E-5
42-43	1.2366483931255777E-4
44-45	1.1586915413378359E-4
46-47	6.304154374691378E-5
48-49	2.2748695794521406E-4
50-51	2.2971123735428153E-4
52-53	2.967376936155494E-4
54-55	0.0015820031323662022
56-57	0.0026010540227570473
58-59	0.0014913916586655734
60-61	0.0029668176425724996
62-63	0.0016309875922863076
64-65	0.0024869911611149855
66-67	4.99139334046304E-4
68-69	5.153580748324177E-4
70-71	2.8651576972563863E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.187313131754476E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	68.0
20-21	202.0
22-23	372.0
24-25	502.0
26-27	1627.0
28-29	4707.0
30-31	12002.0
32-33	17963.0
34-35	25813.0
36-37	39572.0
38-39	43922.0
40-41	41733.0
42-43	48020.0
44-45	56944.0
46-47	58107.0
48-49	54308.0
50-51	50133.0
52-53	49651.0
54-55	54147.0
56-57	56405.0
58-59	55970.0
60-61	55358.0
62-63	52666.0
64-65	56253.0
66-67	60155.0
68-69	61538.0
70-71	63172.0
72-73	55112.0
74-75	54892.0
76-77	57085.0
78-79	59098.0
80-81	57655.0
82-83	53955.0
84-85	52848.0
86-87	54280.0
88-89	56084.0
90-91	56882.0
92-93	55258.0
94-95	62198.0
96-97	175644.0
98-99	1292357.0
100-101	2695507.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.94885204554727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.91750649600652	79.05172820671353
2	6.543016747135626	11.378155901564668
3	1.4554184648581037	3.7964089429591437
4	0.5191681968617542	1.8056431494274487
5	0.21483523698078485	0.9339838617206173
6	0.11259270912797222	0.5873884084425237
7	0.06376525085685922	0.388102075368018
8	0.046836485343503924	0.32579029075726124
9	0.02530096053608017	0.1979900526835678
>10	0.09888535389789577	1.3374227617876884
>50	0.0023343664175497782	0.1347792304426713
>100	3.098294798609484E-4	0.04185921528393287
>500	2.9902497439771143E-5	0.020747902848950475
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.706436593508886E-5	0.0	0.0	0.0
3	0.0	1.706436593508886E-5	0.0	0.0	0.0
4	0.0	5.1193097805266574E-5	0.0	0.0	0.0
5	0.0	5.1193097805266574E-5	0.0	0.0	0.0
6	6.825746374035544E-5	5.1193097805266574E-5	0.0	0.0	0.0
7	6.825746374035544E-5	5.1193097805266574E-5	0.0	0.0	0.0
8	6.825746374035544E-5	5.1193097805266574E-5	0.0	0.0	0.0
9	6.825746374035544E-5	8.532182967544429E-5	0.0	0.0	0.0
10-11	6.825746374035544E-5	8.532182967544429E-5	0.0	1.706436593508886E-5	0.0
12-13	6.825746374035544E-5	8.532182967544429E-5	0.0	1.706436593508886E-5	0.0
14-15	6.825746374035544E-5	1.5357929341579973E-4	0.0	1.706436593508886E-5	0.0
16-17	1.7064365935088858E-4	1.7064365935088858E-4	0.0	1.706436593508886E-5	8.53218296754443E-6
18-19	1.7064365935088858E-4	1.79175842318433E-4	0.0	1.706436593508886E-5	1.706436593508886E-5
20-21	1.7064365935088858E-4	1.8770802528597744E-4	0.0	2.559654890263329E-5	1.706436593508886E-5
22-23	1.7064365935088858E-4	2.133045741886107E-4	0.0	5.972528077281101E-5	1.706436593508886E-5
24-25	1.7064365935088858E-4	3.0715858683159946E-4	0.0	8.532182967544429E-5	1.706436593508886E-5
26-27	1.7064365935088858E-4	3.5835168463686605E-4	0.0	1.2798274451316643E-4	1.706436593508886E-5
28-29	1.7064365935088858E-4	3.5835168463686605E-4	0.0	3.156907697991439E-4	1.706436593508886E-5
30-31	1.7064365935088858E-4	4.607378802473992E-4	0.0	0.0011177159687483203	1.706436593508886E-5
32-33	1.8770802528597744E-4	4.607378802473992E-4	0.0	0.002781491647419484	1.706436593508886E-5
34-35	2.133045741886107E-4	5.204631610202102E-4	0.0	0.005110777597559113	1.706436593508886E-5
36-37	2.303689401236996E-4	5.887206247605656E-4	0.0	0.008600440431284785	1.706436593508886E-5
38-39	2.38901123091244E-4	6.484459055333766E-4	0.0	0.013881861688194786	1.706436593508886E-5
40-41	2.38901123091244E-4	6.996390033386432E-4	0.0	0.0190694289324618	1.706436593508886E-5
42-43	2.38901123091244E-4	7.678964670789986E-4	0.0	0.024649476593235856	1.706436593508886E-5
44-45	2.474333060587884E-4	9.470723093974317E-4	0.0	0.03133017585682314	1.706436593508886E-5
46-47	2.5596548902633286E-4	0.001075055053910598	0.0	0.03864225666000872	1.706436593508886E-5
48-49	2.5596548902633286E-4	0.0010835872368781425	0.0	0.04528882719172583	1.706436593508886E-5
50-51	2.98626403864055E-4	0.0011859734324886756	0.0	0.051781818430027146	1.706436593508886E-5
52-53	3.0715858683159946E-4	0.001211569981391309	0.0	0.05830040621723108	1.706436593508886E-5
54-55	3.0715858683159946E-4	0.0012286343473263978	0.0	0.06467394689398677	1.706436593508886E-5
56-57	3.0715858683159946E-4	0.0012456987132614867	0.0	0.07201162424607499	1.706436593508886E-5
58-59	3.0715858683159946E-4	0.0012456987132614867	0.0	0.07955407398938426	1.706436593508886E-5
60-61	3.156907697991439E-4	0.0012542308962290312	0.0	0.08635422381451717	1.706436593508886E-5
62-63	3.4128731870177717E-4	0.0012798274451316645	0.0	0.09248886336818161	1.706436593508886E-5
64-65	3.4128731870177717E-4	0.0014334067385474642	0.0	0.09902451552132065	1.706436593508886E-5
66-67	3.4128731870177717E-4	0.0014675354704176418	0.0	0.1053724596491737	1.706436593508886E-5
68-69	3.4128731870177717E-4	0.0015016642022878195	0.0	0.11206169109572853	1.706436593508886E-5
70-71	3.4128731870177717E-4	0.0015613894830606306	0.0	0.11803421917300963	1.706436593508886E-5
72-73	4.010125994745882E-4	0.001569921666028175	0.0	0.12242829340129502	1.706436593508886E-5
74-75	4.095447824421326E-4	0.0015955182149308082	0.0	0.12578997349050752	1.706436593508886E-5
76-77	4.266091483772215E-4	0.001629646946800986	0.0	0.12940761906874637	1.706436593508886E-5
78-79	4.436735143123103E-4	0.0016552434957036192	0.0	0.1318136946655939	1.706436593508886E-5
80-81	4.436735143123103E-4	0.0016552434957036192	0.0	0.132410947473322	1.706436593508886E-5
82-83	4.436735143123103E-4	0.0016552434957036192	0.0	0.13297407154917992	1.706436593508886E-5
84-85	4.436735143123103E-4	0.0016552434957036192	0.0	0.1332215048552387	1.706436593508886E-5
86-87	4.436735143123103E-4	0.001689372227573797	0.0	0.13334095541678434	1.706436593508886E-5
88	4.436735143123103E-4	0.0017576296913141523	0.0	0.13335801978271944	1.706436593508886E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6645	0.0	47.93538	1
GTATCAA	11735	0.0	34.267952	1
TCAACGC	14105	0.0	28.15443	4
ATCAACG	14265	0.0	27.809338	3
CAACGCA	14305	0.0	27.67313	5
AACGCAG	14830	0.0	26.664598	6
TATCAAC	15175	0.0	26.417156	2
GTACATG	17310	0.0	23.231337	1
TACATGG	17590	0.0	22.909065	2
ACGCAGA	17355	0.0	22.761047	7
ACATGGG	17640	0.0	22.488672	3
CGCAGAG	17995	0.0	22.021229	8
GTGGTAT	3320	0.0	19.893684	1
CATGGGG	12025	0.0	19.571291	4
TGGTATC	3275	0.0	18.762997	2
GCAGAGT	21455	0.0	18.489397	9
AGAGTAC	18980	0.0	17.629913	10-11
GAGTACT	12540	0.0	17.350351	12-13
CAGAGTA	20815	0.0	17.049685	10-11
ATGGGGG	7145	0.0	16.498447	5
>>END_MODULE