FastQCFastQC Report
Fri 27 May 2016
SRR2139567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12812989
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116100.039.2749331
GTATCAA187700.028.8902971
TCAACGC210250.024.760544
ATCAACG214400.024.2812653
CAACGCA214000.024.2693215
AACGCAG223300.023.3134946
TATCAAC232850.022.6561572
GTACATG309850.021.618981
TACATGG317650.020.5216482
ACATGGG317400.019.9835453
ACGCAGA269450.019.3053027
CGCAGAG282000.018.4026418
CATGGGG242250.017.387664
GTGGTAT71250.015.0953661
GAGTACT180250.015.07722112-13
GCAGAGT345250.014.9009749
GAGTACA232050.014.8213311
AGAGTAC300100.014.79331210-11
ATGGGGG148950.014.6611625
CAGAGTA332050.014.416821510-11
Produced by FastQC (version 0.11.5)