##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139567_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12812989
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1436560196844	33.0	31.0	34.0	30.0	34.0
2	32.277911344495806	34.0	31.0	34.0	30.0	34.0
3	32.332407996291884	34.0	31.0	34.0	30.0	34.0
4	35.95789054372871	37.0	35.0	37.0	35.0	37.0
5	35.820000079606714	37.0	35.0	37.0	35.0	37.0
6	35.83631867630574	37.0	35.0	37.0	35.0	37.0
7	35.749762682228166	37.0	35.0	37.0	33.0	37.0
8	35.71550408729766	37.0	35.0	37.0	33.0	37.0
9	37.34658244067797	39.0	37.0	39.0	34.0	39.0
10-11	37.24421877674288	39.0	37.0	39.0	33.0	39.0
12-13	37.20096766648282	39.0	37.0	39.0	33.0	39.0
14-15	38.403181334191416	40.0	38.0	41.0	33.0	41.0
16-17	38.413289514257755	40.0	38.0	41.0	33.0	41.0
18-19	38.39688495010805	40.0	38.0	41.0	33.5	41.0
20-21	38.35803051475567	40.0	38.0	41.0	34.0	41.0
22-23	38.250726381471175	40.0	38.0	41.0	33.0	41.0
24-25	38.159647914178024	40.0	37.5	41.0	33.0	41.0
26-27	37.984142526076326	40.0	37.0	41.0	33.0	41.0
28-29	37.84442354371819	40.0	37.0	41.0	33.0	41.0
30-31	37.69623444585032	40.0	37.0	41.0	32.0	41.0
32-33	37.9122172079132	40.0	37.0	41.0	33.0	41.0
34-35	37.90241909910205	40.0	37.0	41.0	33.0	41.0
36-37	37.817012332270664	40.0	37.0	41.0	33.0	41.0
38-39	37.706592335598025	40.0	37.0	41.0	33.0	41.0
40-41	37.653567510324166	40.0	37.0	41.0	32.5	41.0
42-43	37.54361441335067	40.0	37.0	41.0	32.0	41.0
44-45	37.58559623299984	39.5	36.5	41.0	32.5	41.0
46-47	37.722582167852046	40.0	37.0	41.0	32.5	41.0
48-49	37.64409016429832	40.0	37.0	41.0	32.0	41.0
50-51	37.51455808979192	40.0	36.0	41.0	32.0	41.0
52-53	37.357192369369976	39.0	36.0	41.0	31.0	41.0
54-55	37.24118920883981	39.0	36.0	41.0	31.0	41.0
56-57	37.065064875019225	39.0	35.0	40.5	31.0	41.0
58-59	36.90956506876647	39.0	35.0	40.0	31.0	41.0
60-61	36.667446706202206	38.0	35.0	40.0	31.0	41.0
62-63	36.3917704211157	38.0	35.0	40.0	30.0	41.0
64-65	36.15482983531301	37.5	34.5	40.0	30.0	41.0
66-67	35.85241312642684	37.0	34.0	40.0	30.0	41.0
68-69	35.520466444901814	36.5	34.0	39.0	29.0	41.0
70-71	35.21008573585135	36.0	34.0	39.0	29.0	40.5
72-73	34.89896708920088	35.5	34.0	38.5	29.0	40.0
74-75	34.50900214979667	35.0	33.0	37.5	29.0	40.0
76-77	33.45556277534705	34.5	31.5	36.5	27.5	39.0
78-79	33.79637249389663	35.0	33.0	36.5	28.5	39.0
80-81	33.67581593269149	35.0	33.0	36.0	29.0	38.5
82-83	33.45491708093192	35.0	33.0	36.0	29.0	37.0
84-85	33.2405570758696	35.0	32.5	35.0	29.0	37.0
86-87	33.03418464881278	34.5	32.0	35.0	28.0	36.5
88-89	32.812283013658366	34.0	32.0	35.0	27.0	36.0
90-91	32.67327987493263	34.0	32.0	35.0	27.0	36.0
92-93	32.583429465328265	34.0	32.0	35.0	27.0	35.5
94-95	32.532329736333686	34.0	32.0	35.0	27.0	35.0
96-97	32.47390453378354	34.0	32.0	35.0	27.0	35.0
98-99	32.60561869016579	34.0	32.0	35.0	28.5	35.0
100-101	31.656527434338948	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	13.0
20	97.0
21	574.0
22	2685.0
23	8770.0
24	22715.0
25	47372.0
26	83254.0
27	130267.0
28	185042.0
29	244193.0
30	307903.0
31	382763.0
32	474035.0
33	601089.0
34	796390.0
35	1101188.0
36	1663532.0
37	2594547.0
38	2739640.0
39	1374602.0
40	52313.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.69567132228085	21.12698293895359	13.61214779783234	25.56519794093322
2	15.178981452662002	22.190676599622442	38.78032935097273	23.85001259674283
3	18.043166976885722	25.867890778646576	30.658623058210694	25.430319186257012
4	12.703200509484148	17.95087942208514	37.61133258825439	31.734587480176323
5	13.167364773356164	37.72866737027559	35.826129250559724	13.277838605808528
6	31.37791658136911	37.578405788063975	17.82367096389453	13.22000666667239
7	26.890782470819257	31.972563154467704	23.094447361189495	18.04220701352354
8	24.971004033485084	36.56493422416893	20.938174535231397	17.525887207114593
9	25.61124496399708	15.530638479436764	20.786024244616147	38.07209231195001
10-11	24.620445705525853	26.382314072071704	28.915981274939046	20.08125894746339
12-13	24.88262496752319	23.920819724421836	28.94641523535219	22.250140072702788
14-15	22.07184834077357	25.526803308736156	25.99960477605967	26.401743574430604
16-17	22.201935294112825	28.426609343812988	26.914204479357366	22.45725088271682
18-19	22.216045764185075	27.905713491208022	28.139999183640914	21.738241560965985
20-21	22.809377222018856	27.200465028789168	27.92890476275961	22.06125298643237
22-23	22.532967652926825	27.171233918464395	27.803288180876056	22.49251024773272
24-25	22.42304240402284	27.2877485826092	27.89300168295028	22.396207330417678
26-27	22.430682727868255	27.380801412190532	27.826547677579978	22.361968182361235
28-29	22.382829521149482	27.35492448386135	27.886345348724056	22.37590064626511
30-31	22.537646089485	27.34732155662601	27.793331915427324	22.321700438461672
32-33	22.311448890528716	27.406594323214843	27.823892485537478	22.45806430071896
34-35	22.449922027997605	27.449646503683105	27.784986575722765	22.315444892596524
36-37	22.452472014862522	27.367620274241943	27.760466865900028	22.41944084499551
38-39	22.34247127283521	27.42409222902515	27.840004200598223	22.393432297541416
40-41	22.53271328531653	27.325218349859682	27.74978718561163	22.392281179212166
42-43	22.44467799224447	27.397847972739786	27.619630307761962	22.537843727253783
44-45	22.476284763587067	27.497346259705047	27.490075175615164	22.536293801092715
46-47	22.601157083652883	27.39777012575933	27.28931848191982	22.711754308667963
48-49	22.496472360642095	27.47044701163526	27.34654142784162	22.686539199881018
50-51	22.476200611946258	27.57109698017826	27.30446720766263	22.648235200212852
52-53	22.59939918166001	27.50690428100712	27.25795337193843	22.635743165394434
54-55	22.580823826285005	27.442813257545463	27.236491146424036	22.7398717697455
56-57	22.633028044630468	27.521470623151316	27.1521058056145	22.693395526603712
58-59	22.55595882533827	27.553027770160192	27.234596397190263	22.65641700731127
60-61	22.6294645778092	27.61589514777423	27.178525708734234	22.57611456568234
62-63	22.62450097155249	27.657796801208868	27.11618322951924	22.601518997719403
64-65	22.586498884714135	27.569863816377282	27.1301270574766	22.713510241431987
66-67	22.631096674641697	27.565572689878547	27.042318976326285	22.761011659153468
68-69	22.597013623484006	27.52076626429728	27.106929978197197	22.775290134021514
70-71	22.570859096422254	27.35369776755161	27.18035786174075	22.895085274285385
72-73	22.64304467287846	27.386645604235916	27.08712638206716	22.883183340818462
74-75	22.542193688862913	27.490795547602957	27.09321372833325	22.873797035200884
76-77	22.65303885121868	27.438570928345158	27.063178431879464	22.845211788556693
78-79	22.55504238457436	27.429490728369483	27.044849084624957	22.9706178024312
80-81	22.591492880631566	27.440495656641616	27.08671079810845	22.88130066461837
82-83	22.653195006324943	27.429658399108714	27.12744594833079	22.78970064623555
84-85	22.62842519703608	27.46064145649768	27.079783550564635	22.831149795901602
86-87	22.556487867133292	27.443445103848617	27.144121437812657	22.855945591205433
88-89	22.57807376792107	27.387632280336803	27.13145406583481	22.90283988590732
90-91	22.562878197761407	27.495700611171074	27.11664413635554	22.82477705471198
92-93	22.581110928697825	27.43039898553052	27.16434506859915	22.824145017172505
94-95	22.3671224720463	27.455753968569454	27.278110590708266	22.899012968675983
96-97	22.357188714027572	27.525563404990866	27.298434399665027	22.81881348131654
98-99	22.507449637613938	28.11503334663391	27.35822480522995	22.019292210522202
100-101	25.535107932870137	35.88319958505925	28.462906446917824	10.118786035152786
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2878.0
1	2567.5
2	3038.5
3	4035.0
4	5187.5
5	7773.5
6	9940.0
7	11395.0
8	12181.0
9	11917.0
10	11080.5
11	10202.0
12	11255.5
13	14843.5
14	22755.5
15	33229.0
16	41072.0
17	45134.5
18	46926.0
19	47357.5
20	46837.0
21	46764.0
22	48759.0
23	54816.5
24	65543.0
25	81248.5
26	101717.0
27	124640.0
28	151301.0
29	180608.5
30	208231.5
31	235753.0
32	265952.0
33	296730.5
34	325967.5
35	356016.5
36	389333.0
37	418466.5
38	442533.5
39	464405.0
40	484049.5
41	503608.0
42	518221.0
43	531808.0
44	546137.5
45	558641.5
46	567424.0
47	571835.0
48	578948.0
49	579371.5
50	571721.0
51	562345.5
52	545168.0
53	520497.5
54	490476.0
55	456563.0
56	421301.5
57	384455.0
58	345014.5
59	299584.5
60	251252.5
61	207552.0
62	168490.5
63	132921.5
64	101001.5
65	76600.5
66	58849.0
67	45501.0
68	35238.5
69	26489.0
70	19643.0
71	14369.5
72	10229.5
73	7115.0
74	4957.0
75	3449.0
76	2393.0
77	1630.5
78	1129.0
79	777.5
80	536.5
81	364.5
82	266.0
83	198.5
84	149.0
85	113.5
86	89.0
87	70.0
88	46.5
89	29.5
90	21.5
91	16.0
92	14.5
93	10.5
94	5.5
95	4.0
96	3.5
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011941007675882653
3	0.0
4	2.263328252291483E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.003234998484740758
18-19	0.0
20-21	0.0021619702870355074
22-23	0.0
24-25	3.279224310420118E-4
26-27	0.0034094437295330857
28-29	0.0
30-31	1.487038134271169E-4
32-33	8.709983559709861E-4
34-35	8.389733612959609E-4
36-37	0.010077969720674585
38-39	0.01163900156305438
40-41	0.02470301484360687
42-43	0.0019592916112076034
44-45	0.0013063132971437993
46-47	0.001954270242643025
48-49	0.00197373044733822
50-51	3.831314216551158E-5
52-53	9.467932328384043E-5
54-55	0.0063829332382192095
56-57	0.0038114233032449994
58-59	0.0037743625262684896
60-61	0.0017320543638361559
62-63	4.08995661980618E-4
64-65	6.987811905060606E-4
66-67	7.565785927222057E-4
68-69	0.0
70-71	9.758205809440488E-6
72-73	1.1397406247661674E-4
74-75	2.566465932127343E-4
76-77	0.0014368150361415226
78-79	0.0020018935312944314
80-81	0.010866346322048758
82-83	0.00970942995247067
84-85	0.012601174738757303
86-87	0.006613092426121313
88-89	0.07951875767496178
90-91	4.075233467214456E-4
92-93	3.5754315650182394E-5
94-95	2.323888967031354E-4
96-97	0.014199875010725841
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	385.0
20-21	1138.0
22-23	2347.0
24-25	4864.0
26-27	7340.0
28-29	13287.0
30-31	29781.0
32-33	45028.0
34-35	66843.0
36-37	101508.0
38-39	105397.0
40-41	105711.0
42-43	122877.0
44-45	144251.0
46-47	146322.0
48-49	138112.0
50-51	127932.0
52-53	130347.0
54-55	146633.0
56-57	153934.0
58-59	153632.0
60-61	149438.0
62-63	145287.0
64-65	154624.0
66-67	166028.0
68-69	161970.0
70-71	159751.0
72-73	153581.0
74-75	156871.0
76-77	160077.0
78-79	168548.0
80-81	169110.0
82-83	161321.0
84-85	164182.0
86-87	173910.0
88-89	184166.0
90-91	196239.0
92-93	206577.0
94-95	258463.0
96-97	335828.0
98-99	1051742.0
100-101	6587607.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.92471840728311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.5261266880201	77.78431944170164
2	6.6429327682528765	11.415842550212842
3	1.5818136083139638	4.077506666015572
4	0.566240382260504	1.946161815862646
5	0.24805760696763368	1.0657140013739217
6	0.1490741379623459	0.7685491995733826
7	0.0821874344328639	0.4943352512182612
8	0.048759446221360185	0.3351713349012351
9	0.03441082661739699	0.2661066528535512
>10	0.11639587717125865	1.5540866191912779
>50	0.0032464153806687525	0.18657053552754235
>100	7.396393316465728E-4	0.09711170965942936
>500	1.5169067406083035E-5	0.008524221908711828
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.804580180315459E-6	0.0	0.0	0.0	0.0
3	7.804580180315459E-6	0.0	0.0	0.0	0.0
4	7.804580180315459E-6	3.1218320721261836E-5	0.0	1.5609160360630918E-5	0.0
5	7.804580180315459E-6	3.90229009015773E-5	0.0	1.5609160360630918E-5	0.0
6	1.0145954234410098E-4	3.90229009015773E-5	0.0	1.5609160360630918E-5	0.0
7	1.0145954234410098E-4	4.682748108189276E-5	0.0	2.341374054094638E-5	0.0
8	1.0926412252441644E-4	5.463206126220822E-5	0.0	2.341374054094638E-5	7.804580180315459E-6
9	1.0926412252441644E-4	1.170687027047319E-4	0.0	3.90229009015773E-5	1.5609160360630918E-5
10-11	1.170687027047319E-4	1.2487328288504735E-4	0.0	5.463206126220822E-5	1.5609160360630918E-5
12-13	1.3267786306536282E-4	1.3658015315552054E-4	0.0	5.463206126220822E-5	1.5609160360630918E-5
14-15	1.4438473333583602E-4	1.4828702342599374E-4	0.0	5.853435135236595E-5	2.7316030631104106E-5
16-17	3.0047633694214523E-4	1.4828702342599374E-4	0.0	6.633893153268141E-5	3.1218320721261836E-5
18-19	3.0437862703230293E-4	1.6389618378662466E-4	0.0	7.024122162283913E-5	3.90229009015773E-5
20-21	3.0437862703230293E-4	1.9511450450788648E-4	0.0	7.80458018031546E-5	5.853435135236595E-5
22-23	3.082809171224606E-4	2.1852824504883285E-4	0.0	1.053618324342587E-4	6.633893153268141E-5
24-25	3.277923675732493E-4	2.380396954996215E-4	0.0	1.560916036063092E-4	7.80458018031546E-5
26-27	3.394992378437225E-4	2.497465657700947E-4	0.0	2.6925801622088334E-4	8.194809189331233E-5
28-29	3.434015279338802E-4	2.497465657700947E-4	0.0	0.0011745893171374766	8.585038198347006E-5
30-31	3.5120610811419567E-4	2.731603063110411E-4	0.0	0.003793025967633313	8.975267207362778E-5
32-33	3.590106882945111E-4	2.8486717658151423E-4	0.0	0.009217209192952557	1.0145954234410098E-4
34-35	3.980335891960884E-4	3.1608549730277613E-4	0.0	0.017267633648947955	1.0926412252441644E-4
36-37	4.1364274955671935E-4	3.551083982043534E-4	0.0	0.028233068802291178	1.0926412252441644E-4
38-39	4.1364274955671935E-4	4.097404594665616E-4	0.0	0.04125501083314752	1.0926412252441644E-4
40-41	4.292519099173503E-4	4.3705649009766575E-4	0.0	0.0527043299576703	1.0926412252441644E-4
42-43	4.7607939099924304E-4	4.6437252072876984E-4	0.0	0.06249127350378588	1.0926412252441644E-4
44-45	4.994931315401895E-4	5.112000018106626E-4	0.0	0.07530249186977371	1.0926412252441644E-4
46-47	5.151022919008204E-4	5.385160324417667E-4	0.0	0.08889807054388324	1.0926412252441644E-4
48-49	5.190045819909781E-4	5.541251928023977E-4	0.0	0.09966448890262841	1.0926412252441644E-4
50-51	5.463206126220822E-4	5.931480937039749E-4	0.0	0.10950996680009636	1.0926412252441644E-4
52-53	5.580274828925554E-4	6.048549639744482E-4	0.0	0.11813012560925479	1.2097099279488962E-4
54-55	5.697343531630285E-4	6.126595441547636E-4	0.0	0.1273863577031089	1.3658015315552054E-4
56-57	5.892458036138172E-4	6.165618342449213E-4	0.0	0.1366855149879548	1.4048244324567827E-4
58-59	6.126595441547636E-4	6.243664144252368E-4	0.0	0.14507153639170375	1.4048244324567827E-4
60-61	6.3607328469571E-4	6.3607328469571E-4	0.0	0.15357072420806728	1.4048244324567827E-4
62-63	6.711938955071295E-4	6.477801549661832E-4	0.0	0.1617109013361363	1.4828702342599374E-4
64-65	6.711938955071295E-4	6.946076360480759E-4	0.0	0.1705573929705239	1.4828702342599374E-4
66-67	6.907053459579181E-4	7.024122162283913E-4	0.0	0.1799658143778942	1.5218931351615146E-4
68-69	6.946076360480759E-4	7.024122162283913E-4	0.0	0.18902302967715026	1.6389618378662466E-4
70-71	7.2192366667918E-4	7.141190864988645E-4	0.0	0.19783830299081656	1.717007639669401E-4
72-73	7.882625982118614E-4	7.258259567693377E-4	0.0	0.20504193049724775	1.8730992432757103E-4
74-75	7.921648883020191E-4	7.297282468594955E-4	0.0	0.211184135099156	1.8730992432757103E-4
76-77	8.038717585724924E-4	7.453374072201263E-4	0.0	0.21764242519836707	1.8730992432757103E-4
78-79	8.116763387528078E-4	7.492396973102841E-4	0.0	0.22323050460747293	1.8730992432757103E-4
80-81	8.116763387528078E-4	7.492396973102841E-4	0.0	0.22620795194626328	1.8730992432757103E-4
82-83	8.116763387528078E-4	7.570442774905996E-4	0.0	0.22805763744899804	1.8730992432757103E-4
84-85	8.389923693839119E-4	7.64848857670915E-4	0.0	0.2295561168436186	1.8730992432757103E-4
86-87	8.546015297445429E-4	7.882625982118614E-4	0.0	0.22984098402020015	1.9121221441772876E-4
88-89	8.741129801953315E-4	7.882625982118614E-4	0.0	0.22991512753191312	2.0291908468820195E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11610	0.0	39.274933	1
GTATCAA	18770	0.0	28.890297	1
TCAACGC	21025	0.0	24.76054	4
ATCAACG	21440	0.0	24.281265	3
CAACGCA	21400	0.0	24.269321	5
AACGCAG	22330	0.0	23.313494	6
TATCAAC	23285	0.0	22.656157	2
GTACATG	30985	0.0	21.61898	1
TACATGG	31765	0.0	20.521648	2
ACATGGG	31740	0.0	19.983545	3
ACGCAGA	26945	0.0	19.305302	7
CGCAGAG	28200	0.0	18.402641	8
CATGGGG	24225	0.0	17.38766	4
GTGGTAT	7125	0.0	15.095366	1
GAGTACT	18025	0.0	15.077221	12-13
GCAGAGT	34525	0.0	14.900974	9
GAGTACA	23205	0.0	14.821331	1
AGAGTAC	30010	0.0	14.793312	10-11
ATGGGGG	14895	0.0	14.661162	5
CAGAGTA	33205	0.0	14.4168215	10-11
>>END_MODULE