FastQCFastQC Report
Fri 27 May 2016
SRR2139566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20149565
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA201400.044.0236741
GTATCAA341400.032.030031
TCAACGC418600.025.2836364
ATCAACG428900.024.7049923
CAACGCA431550.024.5343765
AACGCAG445650.023.7855956
TATCAAC450700.023.728242
GTACATG530350.020.9801521
TACATGG539150.020.213912
ACGCAGA533850.019.8332277
ACATGGG539150.019.7514823
CGCAGAG549850.019.2486848
CATGGGG387400.017.1039034
AGAGTAC576950.016.6472910-11
GCAGAGT652250.015.901459
GAGTACT367450.015.495712-13
GTGGTAT110750.015.28941
CAGAGTA633750.014.96213110-11
AGTACTT419700.014.17418712-13
ATGGGGG239800.014.1390635
Produced by FastQC (version 0.11.5)