##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20149565
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64894358761591	31.0	31.0	34.0	30.0	34.0
2	31.867216240152082	31.0	31.0	34.0	30.0	34.0
3	31.883462049925146	31.0	31.0	34.0	30.0	34.0
4	35.63250323270006	37.0	35.0	37.0	33.0	37.0
5	35.45259314531108	37.0	35.0	37.0	33.0	37.0
6	35.44086117988155	37.0	35.0	37.0	33.0	37.0
7	35.298742925715764	37.0	35.0	37.0	32.0	37.0
8	35.28016446012606	37.0	35.0	37.0	32.0	37.0
9	36.80724720359968	39.0	37.0	39.0	32.0	39.0
10-11	36.72712770722346	39.0	35.0	39.0	32.0	39.0
12-13	36.654214148047366	39.0	35.0	39.0	32.0	39.0
14-15	37.72005748014908	39.0	37.0	41.0	32.0	41.0
16-17	37.75594088507618	39.0	37.0	41.0	32.0	41.0
18-19	37.78385751751961	39.5	37.0	41.0	32.0	41.0
20-21	37.75881929578273	40.0	37.0	41.0	32.0	41.0
22-23	37.686236159316564	39.0	37.0	41.0	32.0	41.0
24-25	37.58829594863049	39.0	36.0	41.0	32.0	41.0
26-27	37.41181287576973	39.0	36.0	41.0	32.0	41.0
28-29	37.2644860796332	39.0	36.0	40.5	31.0	41.0
30-31	37.121579082095046	39.0	36.0	40.0	31.0	41.0
32-33	37.35191861815119	39.0	36.0	40.5	31.0	41.0
34-35	37.35523245715528	39.0	36.0	41.0	31.0	41.0
36-37	37.24089975554891	39.0	36.0	41.0	31.0	41.0
38-39	37.12888762720871	39.0	36.0	41.0	31.0	41.0
40-41	37.10681593014511	39.0	36.0	40.5	31.0	41.0
42-43	37.03347419628323	39.0	35.5	40.0	31.0	41.0
44-45	37.007798966258235	39.0	35.5	40.0	31.0	41.0
46-47	37.017571953951354	39.0	35.5	40.0	31.0	41.0
48-49	36.93558414390051	39.0	35.0	40.0	30.0	41.0
50-51	36.84828652806854	39.0	35.0	40.0	30.0	41.0
52-53	36.71831085935429	39.0	35.0	40.0	30.0	41.0
54-55	36.60201757909542	38.5	35.0	40.0	30.0	41.0
56-57	36.42173491982919	38.0	35.0	40.0	30.0	41.0
58-59	36.26604150729041	38.0	34.5	40.0	29.0	41.0
60-61	36.098253002239176	38.0	34.0	40.0	29.0	41.0
62-63	35.864678731184895	37.5	34.0	40.0	29.0	41.0
64-65	35.64459235338025	37.0	34.0	40.0	29.0	41.0
66-67	35.38861253306433	37.0	34.0	39.5	28.5	41.0
68-69	35.10972422829158	36.0	34.0	39.0	28.0	40.5
70-71	34.82582474974925	36.0	33.0	39.0	28.0	40.0
72-73	34.521572679850564	35.0	33.0	38.5	28.0	40.0
74-75	34.20067858096509	35.0	33.0	37.5	28.0	39.5
76-77	33.15765171453104	34.5	31.5	36.0	26.5	39.0
78-79	33.53018143908106	35.0	32.0	36.0	27.0	39.0
80-81	33.46451188750912	35.0	32.0	36.0	28.0	38.5
82-83	33.28636836735067	35.0	32.0	36.0	28.0	37.0
84-85	33.064320534628095	35.0	32.0	35.0	27.0	37.0
86-87	32.905770070091634	34.0	32.0	35.0	27.0	36.5
88-89	32.72750782009186	34.0	32.0	35.0	27.0	36.0
90-91	32.6460688870323	34.0	32.0	35.0	27.0	36.0
92-93	32.61512322872373	34.0	32.0	35.0	27.0	36.0
94-95	32.61268318896702	34.0	32.0	35.0	27.0	35.0
96-97	32.531090030252415	34.0	32.0	35.0	27.0	35.0
98-99	32.67623893066852	34.0	32.0	35.0	28.5	35.0
100-101	32.2103998276298	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	11.0
19	47.0
20	254.0
21	1365.0
22	6120.0
23	20327.0
24	51554.0
25	104997.0
26	182681.0
27	278666.0
28	387911.0
29	502188.0
30	621683.0
31	753425.0
32	914477.0
33	1124326.0
34	1426173.0
35	1851036.0
36	2574576.0
37	3702244.0
38	3815254.0
39	1773268.0
40	56980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.26850356322829	22.044729005315997	14.492228492277626	25.19453893917809
2	14.708252944161899	22.82951222347192	39.34271113529114	23.11952369707504
3	17.283301153151445	26.957758145151022	31.32477053474852	24.434170166949013
4	12.400734209398564	18.584301943987377	38.35313070034018	30.661833146273874
5	12.735029267381206	38.20463617949073	36.12510245258396	12.935232100544106
6	30.300197547688995	38.5436956083171	18.162466534637346	12.993640309356556
7	25.973215798951493	32.67027352699674	23.8414030278073	17.51510764624447
8	24.38721629970672	37.07909823363432	21.322018614297626	17.21166685236133
9	25.542223864386155	15.776107325393873	21.19905814343883	37.48261066678115
10-11	24.12093759840473	26.742363917037416	29.461956126596284	19.674742357961573
12-13	24.56558033803668	24.478158299796945	29.394520124600348	21.561741237566025
14-15	21.793130521676275	25.9060232813959	26.64302926638863	25.657816930539195
16-17	21.621891092934263	28.813495477445787	27.50369548920783	22.060917940412114
18-19	21.708708030742827	28.19870397654411	28.89391294991668	21.19867504279639
20-21	22.3738359799993	27.493201228836263	28.65525085894757	21.47771193221687
22-23	22.0918672474017	27.415536488935693	28.548578382551266	21.94401788111134
24-25	21.957051966878762	27.544743679042345	28.582399015522874	21.91580533855602
26-27	21.93538007500109	27.618520861594693	28.539366082263136	21.90673298114108
28-29	21.910173205897163	27.628055408788683	28.592219328857592	21.869552056456563
30-31	22.086711741476844	27.620708170395464	28.454002802329732	21.83857728579796
32-33	21.91301036885739	27.744760350891568	28.395398981937504	21.946830298313543
34-35	22.050824776607335	27.722960007203273	28.435972601096974	21.790242615092417
36-37	22.0310021199027	27.6665442659309	28.383012439712758	21.919441174453645
38-39	21.905785162994075	27.73968740929451	28.45366555000959	21.90086187770183
40-41	22.048656052863176	27.631133241809362	28.356138612362102	21.96407209296536
42-43	21.974522012436516	27.753808758543396	28.14784444134442	22.123824787675662
44-45	22.02854325816112	27.794711880104096	28.03216140435998	22.144583457374814
46-47	22.116574467789178	27.74311523732483	27.853673198819905	22.28663709606609
48-49	22.034825749882252	27.85908380966037	27.82052281822811	22.285567622229273
50-51	22.066767296733474	27.94408910793323	27.7138196237335	22.275323971599796
52-53	22.18854102106421	27.82168317630191	27.611321071924987	22.378454730708892
54-55	22.15887159584916	27.742007605950747	27.672775613493776	22.42634518470631
56-57	22.14876033988728	27.81648157131398	27.628145584509067	22.406612504289676
58-59	22.088010015971584	27.823756896463685	27.634014823706405	22.454218263858323
60-61	22.159583069182506	27.811337816461023	27.656436139249863	22.372642975106608
62-63	22.171230520914467	27.887241553562614	27.61672674771049	22.324801177812425
64-65	22.087487345071946	27.82808511426589	27.635800863459302	22.448626677202867
66-67	22.163605666545376	27.96011212371068	27.39223182807762	22.484050381666325
68-69	22.148960446231943	27.925161776587494	27.421671824654297	22.50420595252627
70-71	22.106152574474514	27.72346304629246	27.551260358487077	22.61912402074595
72-73	22.177206223027916	27.762555486878387	27.413685532542527	22.646552757551174
74-75	22.10396408676424	27.794520981797525	27.435004458061428	22.666510473376807
76-77	22.30970000714145	27.757249054329204	27.34939938830387	22.58365155022548
78-79	22.24634564621272	27.74931233679647	27.30617412225645	22.698167894734357
80-81	22.2662249453313	27.735023638845302	27.284758468665988	22.713992947157415
82-83	22.32478319733364	27.70409234410215	27.404700114361276	22.566424344202936
84-85	22.320754997793514	27.75945203956141	27.334790000776447	22.58500296186863
86-87	22.26987787615772	27.719632252951737	27.417250011498446	22.593239859392096
88-89	22.26034860813523	27.67714727134579	27.463384055294497	22.599120065224483
90-91	22.32436219888216	27.696320279260263	27.50804651150127	22.471271010356304
92-93	22.409806599208444	27.606225542677308	27.51311080150093	22.47085705661332
94-95	22.183408074701237	27.636435915233758	27.61001465357188	22.570141356493124
96-97	22.135104976649664	27.64624097197818	27.69730497261052	22.521349078761638
98-99	22.276156783558022	28.233532699524755	27.793416401538764	21.696894115378463
100-101	26.21382802114191	35.491523715004746	28.55195572818848	9.742692535664865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5171.0
1	4582.5
2	5621.0
3	7733.5
4	10156.0
5	15037.0
6	19322.5
7	23109.5
8	25631.5
9	25666.5
10	24425.5
11	22902.0
12	24922.5
13	32673.0
14	50378.0
15	74135.0
16	92209.0
17	100561.0
18	102989.5
19	102450.0
20	99687.0
21	98265.5
22	101304.5
23	112313.0
24	132784.5
25	163122.0
26	201560.5
27	242036.5
28	284365.5
29	330240.5
30	373022.5
31	415387.5
32	461572.5
33	507991.5
34	553392.0
35	598132.5
36	647873.5
37	690307.5
38	723394.0
39	756216.5
40	785364.5
41	810020.5
42	825176.5
43	841149.5
44	859847.0
45	873209.5
46	880002.0
47	878081.0
48	874415.5
49	867016.0
50	854374.0
51	832723.5
52	799415.5
53	757484.0
54	709181.5
55	660014.5
56	606101.0
57	548186.5
58	489871.5
59	425352.5
60	358696.0
61	294667.0
62	235607.0
63	184177.0
64	138736.0
65	104681.0
66	80269.0
67	62010.5
68	47884.0
69	35215.5
70	25598.5
71	18594.0
72	12968.5
73	8959.5
74	6163.5
75	4191.0
76	2882.5
77	1948.5
78	1303.5
79	883.0
80	624.5
81	444.0
82	333.5
83	236.5
84	163.0
85	110.5
86	85.5
87	73.0
88	48.5
89	32.5
90	21.0
91	13.5
92	11.5
93	6.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0035087606109610804
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.106896352353015E-4
14-15	0.0
16-17	0.0
18-19	4.1688244882705905E-4
20-21	0.0
22-23	0.0015536724075275775
24-25	2.159894615017777E-4
26-27	0.0053947915671857085
28-29	4.1006192556381963E-4
30-31	0.0
32-33	4.992239688210668E-6
34-35	2.3061646086152893E-4
36-37	0.022170019912658344
38-39	0.009774373769712396
40-41	0.014067970521006596
42-43	0.0018551412441323385
44-45	0.0032517659761935095
46-47	0.0
48-49	7.284735135901169E-4
50-51	1.0880885477755471E-5
52-53	5.5013165475695815E-6
54-55	0.004731138915584021
56-57	0.009730811022272098
58-59	0.012826397519009066
60-61	0.006691549789524022
62-63	0.007851895847720666
64-65	8.965465975047268E-4
66-67	1.0847404023374469E-4
68-69	6.121755721989769E-6
70-71	1.3687847334959016E-4
72-73	9.483854796239854E-6
74-75	1.766647059025702E-4
76-77	3.591949331309651E-5
78-79	9.96637312486427E-6
80-81	0.0021722862561681764
82-83	0.0022472611074413123
84-85	0.01012577120382995
86-87	0.011429183655180242
88-89	0.04721900976544292
90-91	4.661974060328779E-4
92-93	7.752500171757978E-4
94-95	3.918905462244892E-6
96-97	0.00977717075144801
98-99	1.1072417271369701E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	690.0
20-21	2172.0
22-23	4348.0
24-25	9059.0
26-27	11152.0
28-29	20365.0
30-31	53800.0
32-33	76487.0
34-35	111800.0
36-37	161495.0
38-39	170203.0
40-41	182078.0
42-43	205689.0
44-45	241032.0
46-47	241865.0
48-49	223570.0
50-51	208925.0
52-53	195242.0
54-55	215149.0
56-57	226438.0
58-59	231872.0
60-61	228707.0
62-63	225560.0
64-65	243686.0
66-67	257859.0
68-69	262119.0
70-71	259156.0
72-73	250186.0
74-75	254245.0
76-77	256096.0
78-79	272766.0
80-81	273090.0
82-83	262071.0
84-85	261596.0
86-87	274132.0
88-89	292012.0
90-91	311721.0
92-93	322886.0
94-95	386563.0
96-97	481273.0
98-99	1554201.0
100-101	1.0426209E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.06637812545455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.35571003166648	76.86232995347669
2	6.5967783391527925	11.22328081216359
3	1.6031084624379854	4.091118919255972
4	0.6367946540217914	2.1667925930914285
5	0.29922905339787875	1.2727165901232893
6	0.15136681194348026	0.7725735876257197
7	0.10439956931068255	0.6216625267381987
8	0.06353826044323017	0.4323975750637944
9	0.04352615288714206	0.3332350961859575
>10	0.14105746520448578	1.8571675222151391
>50	0.00342053590272674	0.19676476052105407
>100	0.0010069772686946243	0.12899886441570216
>500	5.310814670655148E-5	0.028741229115645927
>1k	1.0578215928173405E-5	0.012219970007805616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.925772591120454E-6
2	0.0	4.962886295560227E-6	0.0	0.0	9.925772591120454E-6
3	0.0	4.962886295560227E-6	0.0	4.962886295560227E-6	9.925772591120454E-6
4	0.0	2.4814431477801134E-5	0.0	1.488865888668068E-5	1.488865888668068E-5
5	0.0	2.4814431477801134E-5	0.0	2.977731777336136E-5	1.488865888668068E-5
6	2.977731777336136E-5	2.4814431477801134E-5	0.0	3.474020406892159E-5	2.977731777336136E-5
7	2.977731777336136E-5	2.4814431477801134E-5	0.0	3.9703090364481816E-5	3.474020406892159E-5
8	2.977731777336136E-5	3.474020406892159E-5	0.0	4.962886295560227E-5	3.474020406892159E-5
9	2.977731777336136E-5	8.933195332008408E-5	0.0	6.451752184228295E-5	3.9703090364481816E-5
10-11	3.474020406892159E-5	8.933195332008408E-5	0.0	6.699896499006306E-5	3.9703090364481816E-5
12-13	3.474020406892159E-5	8.933195332008408E-5	0.0	7.196185128562329E-5	4.218453351226193E-5
14-15	3.474020406892159E-5	1.3896081627568635E-4	0.0	8.933195332008408E-5	7.196185128562329E-5
16-17	5.4591749251162496E-5	1.488865888668068E-4	0.0	1.0918349850232498E-4	7.940618072896363E-5
18-19	5.7073192398942604E-5	1.9107112237906873E-4	0.0	1.7121957719682784E-4	7.940618072896363E-5
20-21	6.699896499006306E-5	2.2581132644799033E-4	0.0	3.2258760921141476E-4	7.940618072896363E-5
22-23	7.44432944334034E-5	2.456628716302312E-4	0.0	6.402123321272693E-4	9.18133964678642E-5
24-25	7.940618072896363E-5	2.754401894035926E-4	0.0	0.0016104566029092936	1.1414638479788521E-4
26-27	8.436906702452385E-5	2.928102914380534E-4	0.0	0.004392154371570801	1.1910927109344544E-4
28-29	8.436906702452385E-5	3.076989503247341E-4	0.0	0.023298269714507484	1.1910927109344544E-4
30-31	8.685051017230396E-5	3.325133818025352E-4	0.0	0.03719931422837168	1.1910927109344544E-4
32-33	1.0173916905898465E-4	3.325133818025352E-4	0.0	0.0503857031156752	1.4392370257124658E-4
34-35	1.1414638479788522E-4	3.7966080161035734E-4	0.0	0.06918015351696177	1.4392370257124658E-4
36-37	1.1414638479788522E-4	4.292896645659596E-4	0.0	0.0940318066419796	1.5384947516236704E-4
38-39	1.1910927109344544E-4	4.5162265289598065E-4	0.0	0.1340376330704906	1.5384947516236704E-4
40-41	1.1910927109344544E-4	4.714741980782215E-4	0.0	0.2021532474770547	1.5384947516236704E-4
42-43	1.2407215738900566E-4	4.987700727038028E-4	0.0	0.23626564642958792	1.5384947516236704E-4
44-45	1.2655360053678578E-4	5.508803788071852E-4	0.0	0.28392424352585277	1.5384947516236704E-4
46-47	1.3399792998012612E-4	5.756948102849863E-4	0.0	0.3349600847462464	1.637752477534875E-4
48-49	1.3399792998012612E-4	5.781762534327664E-4	0.0	0.37626370594104636	1.8362679293572838E-4
50-51	1.5384947516236704E-4	6.501381047183898E-4	0.0	0.41355483356588596	1.8362679293572838E-4
52-53	1.687381340490477E-4	6.600638773095102E-4	0.0	0.461853642994278	2.1340411070908973E-4
54-55	1.687381340490477E-4	6.650267636050704E-4	0.0	0.5561484826099223	2.456628716302312E-4
56-57	1.687381340490477E-4	6.749525361961908E-4	0.0	0.6505897273712857	2.4814431477801133E-4
58-59	1.687381340490477E-4	6.79915422491751E-4	0.0	0.7270454721975388	2.4814431477801133E-4
60-61	1.687381340490477E-4	6.923226382306516E-4	0.0	0.776540337223161	2.5310720107357156E-4
62-63	1.7370102034460793E-4	6.997669676739919E-4	0.0	0.8184990594089748	2.580700873691318E-4
64-65	1.7370102034460793E-4	7.866174778462959E-4	0.0	0.8376632448392807	2.605515305169119E-4
66-67	1.7618246349238804E-4	7.940618072896363E-4	0.0	0.8477180524740857	2.6799585996025224E-4
68-69	1.7866390664016815E-4	8.039875798807567E-4	0.0	0.8573460518874725	2.729587462558125E-4
70-71	1.8114534978794827E-4	8.238391250629976E-4	0.0	0.8664281338083477	2.779216325513727E-4
72-73	1.9107112237906873E-4	8.31283454506338E-4	0.0	0.8738302787181758	2.928102914380534E-4
74-75	1.9603400867462895E-4	8.511349996885789E-4	0.0	0.8805003978994088	2.928102914380534E-4
76-77	1.9851545182240907E-4	8.784308743141602E-4	0.0	0.8866047480429479	2.977731777336136E-4
78-79	2.034783381179693E-4	8.982824194964011E-4	0.0	0.8918157786532861	3.0273606402917385E-4
80-81	2.0844122441352953E-4	8.982824194964011E-4	0.0	0.8949448784626368	3.076989503247341E-4
82-83	2.0844122441352953E-4	9.007638626441812E-4	0.0	0.8968977742199398	3.27550495506975E-4
84-85	2.0844122441352953E-4	9.032453057919613E-4	0.0	0.8987712637965137	3.27550495506975E-4
86-87	2.0844122441352953E-4	9.106896352353017E-4	0.0	0.8990293338838828	3.27550495506975E-4
88-89	2.1836699700464998E-4	9.379855098608828E-4	0.0	0.8990814441899863	3.27550495506975E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20140	0.0	44.023674	1
GTATCAA	34140	0.0	32.03003	1
TCAACGC	41860	0.0	25.283636	4
ATCAACG	42890	0.0	24.704992	3
CAACGCA	43155	0.0	24.534376	5
AACGCAG	44565	0.0	23.785595	6
TATCAAC	45070	0.0	23.72824	2
GTACATG	53035	0.0	20.980152	1
TACATGG	53915	0.0	20.21391	2
ACGCAGA	53385	0.0	19.833227	7
ACATGGG	53915	0.0	19.751482	3
CGCAGAG	54985	0.0	19.248684	8
CATGGGG	38740	0.0	17.103903	4
AGAGTAC	57695	0.0	16.64729	10-11
GCAGAGT	65225	0.0	15.90145	9
GAGTACT	36745	0.0	15.4957	12-13
GTGGTAT	11075	0.0	15.2894	1
CAGAGTA	63375	0.0	14.962131	10-11
AGTACTT	41970	0.0	14.174187	12-13
ATGGGGG	23980	0.0	14.139063	5
>>END_MODULE