FastQCFastQC Report
Fri 27 May 2016
SRR2139565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14620144
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156750.044.0402951
GTATCAA265450.031.9651781
TCAACGC331200.025.0185894
ATCAACG336250.024.6307833
CAACGCA338100.024.5325
AACGCAG345800.024.0561146
TATCAAC353550.023.724542
GTACATG397550.021.139571
TACATGG402050.020.509522
ACGCAGA410250.020.2178737
ACATGGG405250.019.9065743
CGCAGAG425100.019.5116028
CATGGGG267200.016.9399664
AGAGTAC446350.016.67898410-11
GCAGAGT492250.016.6604299
GAGTACT286650.016.09513712-13
CAGAGTA485550.015.34914210-11
GAGTACA290150.014.5800791
AGTACTT326800.014.38455912-13
GTGGTAT80850.014.2474281
Produced by FastQC (version 0.11.5)