FastQCFastQC Report
Fri 27 May 2016
SRR2139564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12345346
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125400.039.2705041
GTATCAA200500.029.087771
TCAACGC240550.023.685854
ATCAACG241250.023.5496463
CAACGCA244100.023.3413835
AACGCAG250600.022.6872376
TATCAAC257600.022.3873462
ACCGAGG178050.021.38253892-93
GTACATG303850.020.962051
GACCGAG178800.020.73696190-91
AGACCGA181200.020.33208790-91
TACATGG311900.020.0948142
GAGACCG183500.020.04131788-89
CGAGACC187150.019.73709188-89
ACATGGG310800.019.5761153
ACGAGAC197000.019.41233486-87
ACGCAGA298900.018.9939217
GCCCACG195800.018.7079182-83
CACGAGA201450.018.49836786-87
CCCACGA197500.018.48159284-85
Produced by FastQC (version 0.11.5)