##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139564_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12345346
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.810654638598223	31.0	31.0	34.0	30.0	34.0
2	31.956337716253557	31.0	31.0	34.0	30.0	34.0
3	32.02163608861186	33.0	31.0	34.0	30.0	34.0
4	35.72784375585747	37.0	35.0	37.0	33.0	37.0
5	35.57844583699801	37.0	35.0	37.0	33.0	37.0
6	35.5746027693351	37.0	35.0	37.0	33.0	37.0
7	35.450927823327106	37.0	35.0	37.0	33.0	37.0
8	35.44432241915293	37.0	35.0	37.0	33.0	37.0
9	37.04321750074887	39.0	37.0	39.0	33.0	39.0
10-11	36.915131499757074	39.0	37.0	39.0	33.0	39.0
12-13	36.86890306679132	39.0	37.0	39.0	33.0	39.0
14-15	38.02140340983557	40.0	37.0	41.0	33.0	41.0
16-17	38.008577442867946	40.0	37.0	41.0	33.0	41.0
18-19	38.023663532800136	40.0	37.0	41.0	33.0	41.0
20-21	38.004828724548645	40.0	37.0	41.0	33.0	41.0
22-23	37.912239783601066	40.0	37.0	41.0	32.5	41.0
24-25	37.808913728598185	40.0	37.0	41.0	32.0	41.0
26-27	37.63256821262456	39.5	37.0	41.0	32.0	41.0
28-29	37.491512071136995	39.0	36.0	41.0	32.0	41.0
30-31	37.35670444150503	39.0	36.0	41.0	31.5	41.0
32-33	37.532713400747625	39.0	36.5	41.0	32.0	41.0
34-35	37.49874461596316	39.5	36.0	41.0	32.0	41.0
36-37	37.365658528435574	39.0	36.0	41.0	31.0	41.0
38-39	37.19695807889458	39.0	36.0	41.0	31.0	41.0
40-41	37.137567852082036	39.0	36.0	40.0	31.0	41.0
42-43	37.023760269369845	39.0	36.0	40.0	31.0	41.0
44-45	37.090558255376784	39.0	35.5	40.0	31.0	41.0
46-47	37.24720314265177	39.0	36.0	41.0	31.0	41.0
48-49	37.17035477346192	39.0	36.0	41.0	31.0	41.0
50-51	37.02264751089325	39.0	35.5	41.0	31.0	41.0
52-53	36.882064639563126	39.0	35.0	40.0	30.5	41.0
54-55	36.74368222475033	39.0	35.0	40.0	30.0	41.0
56-57	36.57935977226582	38.5	35.0	40.0	30.0	41.0
58-59	36.43129793930852	38.0	35.0	40.0	30.0	41.0
60-61	36.19159275344184	38.0	34.5	40.0	29.5	41.0
62-63	35.918700585534445	38.0	34.0	40.0	29.0	41.0
64-65	35.697322505614	37.0	34.0	40.0	29.0	41.0
66-67	35.43986857248834	37.0	34.0	39.5	29.0	41.0
68-69	35.14526261876577	36.0	34.0	39.0	28.5	40.5
70-71	34.83757668836907	36.0	33.0	39.0	28.0	40.0
72-73	34.506023316270806	35.0	33.0	38.0	28.0	40.0
74-75	34.174619707349464	35.0	33.0	37.5	28.0	39.5
76-77	33.06463473640561	34.0	31.5	36.0	26.0	39.0
78-79	33.457538293606106	35.0	32.0	36.0	27.0	39.0
80-81	33.33412509939512	35.0	32.0	36.0	27.0	38.5
82-83	33.094724742570534	35.0	32.0	36.0	27.0	37.0
84-85	32.905818959046215	34.0	32.0	35.0	27.0	37.0
86-87	32.70901824700546	34.0	32.0	35.0	27.0	36.5
88-89	32.49162002782006	34.0	31.5	35.0	26.0	36.0
90-91	32.36284557676811	34.0	31.0	35.0	26.0	36.0
92-93	32.30165894583095	34.0	31.0	35.0	26.0	35.0
94-95	32.26634743008124	34.0	31.0	35.0	27.0	35.0
96-97	32.17804036904941	34.0	31.0	35.0	26.5	35.0
98-99	32.34783133405443	34.0	31.0	35.0	27.0	35.0
100-101	31.561773100747452	33.0	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	20.0
20	140.0
21	756.0
22	3465.0
23	11400.0
24	28670.0
25	59400.0
26	104490.0
27	159526.0
28	222817.0
29	289901.0
30	362139.0
31	441515.0
32	538718.0
33	669145.0
34	863731.0
35	1144683.0
36	1641696.0
37	2366009.0
38	2313968.0
39	1088523.0
40	34632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.76383051556433	21.675091163909055	14.102666705331709	25.45841161519491
2	14.860584987685716	22.563218245555372	38.88517888844498	23.69101787831393
3	17.55898943618105	26.430680841185012	31.198866358221146	24.81146336441279
4	12.555759879066978	18.403890826551155	37.9083016385284	31.13204765585347
5	12.843317635649903	38.06070724951735	36.10977772514436	12.986197389688389
6	30.716903357751175	38.247627891514746	17.94509445097772	13.09037429975636
7	26.38910241964867	32.466801659507965	23.384480272970883	17.759615647872486
8	24.8271129865457	36.76675404642365	21.035919122882422	17.370213844148232
9	25.59306964746067	15.514939799986166	20.876976635567768	38.015013916985396
10-11	24.30374976934628	26.524011558687782	29.265615560714135	19.906623111251804
12-13	24.7252203380934	24.167710649827068	29.142370736308244	21.96469827577129
14-15	21.911026228021473	25.68172653889166	26.25792748133588	26.149319751750983
16-17	21.896101575443897	28.592199845998646	27.163325353538088	22.348373225019373
18-19	21.934760938829196	28.013551516710688	28.453486961146616	21.598200583313503
20-21	22.547507176215465	27.322662956057613	28.225434891679235	21.90439497604769
22-23	22.36208220785134	27.35729806544026	28.057338412653145	22.223281314055253
24-25	22.20852476478963	27.45628125741282	28.15633465602066	22.178859321776898
26-27	22.23924615061217	27.543638625497067	28.066002768595638	22.151112455295127
28-29	22.110665274650636	27.543824768282484	28.117812273410237	22.227697683656647
30-31	22.38608387797793	27.46603544031842	28.039456632070813	22.108424049632834
32-33	22.272206605074956	27.566791839370214	28.02761272880842	22.133388826746412
34-35	22.204493360308753	27.59182557169063	28.167734267414897	22.03594680058572
36-37	22.30557786627306	27.536266837445694	27.97310065433136	22.185054641949883
38-39	22.14900027191576	27.575630443071326	28.093023050796685	22.182346234216237
40-41	22.287864487443603	27.464996223258503	28.050682771588665	22.19645651770923
42-43	22.24132734972034	27.54593376160065	27.882550108749587	22.33018877992943
44-45	22.24721713301054	27.653112079117815	27.731556085815818	22.368114702055827
46-47	22.348515625810002	27.49307740890792	27.553151066812813	22.60525589846927
48-49	22.28380787303798	27.599835578936215	27.582731816609357	22.533624731416452
50-51	22.231395834177242	27.714363604162934	27.498223958983402	22.556016602676426
52-53	22.31904846037003	27.649638350313175	27.45960076953956	22.57171241977724
54-55	22.332260833177735	27.602601539710896	27.4135732736005	22.65156435351087
56-57	22.3956927367937	27.6585956597097	27.34005829751331	22.605653305983292
58-59	22.26692146213447	27.72032414179902	27.527472819789295	22.485281576277217
60-61	22.3016352379562	27.586605790570832	27.483318042774595	22.628440928698367
62-63	22.49455299208298	27.696856107332312	27.44808872560065	22.360502174984056
64-65	22.128824489440884	27.63016512574937	27.427163894326416	22.81384649048333
66-67	22.182264850415933	27.71282921414075	27.24368719444049	22.861218741002826
68-69	22.168932732582718	27.941489070454185	27.316246393758675	22.573331803204418
70-71	22.141033123752724	27.713999090491885	27.384616260413104	22.760351525342283
72-73	22.205253818588126	27.728760176953028	27.009928530571486	23.05605747388736
74-75	22.168719146014055	27.734192140118495	27.02765338111341	23.069435332754036
76-77	22.332329477559977	27.41573512299035	27.248687373307046	23.003248026142632
78-79	22.255118975726955	27.40805285589535	27.19133543030677	23.14549273807093
80-81	22.600079384726875	27.45248879416813	26.88335285681291	23.064078964292086
82-83	22.61566931835457	27.740124627508884	26.99293564008442	22.65127041405213
84-85	22.3351084444572	27.468253261946113	26.894472275252273	23.30216601834441
86-87	22.22969505814166	27.830854232376378	26.960999348696298	22.978451360785662
88-89	22.591463195712688	27.443135443319743	26.97880040498718	22.986600955980382
90-91	22.632979519234727	27.849668650758076	26.980022605803843	22.537329224203347
92-93	22.313355708661206	27.743728926645066	27.003257147063486	22.93965821763024
94-95	22.40213067224929	27.40761643249195	27.196343455158956	22.993909440099806
96-97	22.3317077619695	27.841797629898014	27.2724055136225	22.554089094509987
98-99	22.58051856131352	28.17361682404095	27.436157404791405	21.809707209854125
100-101	25.90497785990815	35.78543687278898	28.333447033282877	9.976138234019995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3920.0
1	3385.0
2	3734.0
3	4801.5
4	6085.5
5	9059.0
6	11517.0
7	13347.5
8	14454.5
9	14208.0
10	13401.5
11	12396.0
12	13298.5
13	17447.5
14	27175.5
15	40150.5
16	49867.5
17	54552.0
18	56129.0
19	56231.0
20	55302.5
21	54860.5
22	56672.0
23	63232.0
24	75096.0
25	91508.0
26	112569.0
27	135963.0
28	161179.5
29	188322.0
30	214234.5
31	238897.0
32	266225.0
33	294949.0
34	323080.0
35	352108.0
36	382348.5
37	408329.5
38	430475.5
39	451008.0
40	468897.5
41	484883.5
42	496703.5
43	509080.5
44	522946.0
45	535762.5
46	544574.0
47	547396.5
48	548059.5
49	548810.0
50	546672.0
51	534675.5
52	513105.5
53	490374.0
54	465107.0
55	432876.5
56	398609.5
57	362813.0
58	324377.5
59	280655.5
60	233659.5
61	190187.0
62	152698.5
63	119757.0
64	90634.0
65	68901.5
66	52633.0
67	40371.0
68	31306.5
69	23362.0
70	17267.0
71	12788.5
72	9104.5
73	6241.0
74	4307.5
75	3037.0
76	2102.5
77	1425.0
78	976.5
79	695.5
80	492.5
81	329.0
82	234.5
83	163.5
84	113.5
85	86.5
86	72.0
87	59.0
88	42.5
89	28.0
90	17.5
91	8.0
92	4.5
93	3.0
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0027054729774280933
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	9.72026219435243E-5
20-21	0.001956310523103663
22-23	4.415488740523966E-4
24-25	3.6468909950359736E-5
26-27	3.081162981724122E-4
28-29	0.003635447987410314
30-31	3.779605743228786E-4
32-33	4.076399886676083E-5
34-35	0.002280425896762131
36-37	0.05575736858842796
38-39	0.03134011999287645
40-41	0.042650043620983256
42-43	8.343130158632551E-4
44-45	0.0024883884130185116
46-47	0.0015052892480529277
48-49	6.106880156167425E-4
50-51	4.446494179450189E-6
52-53	9.84780775208634E-4
54-55	0.002373710852187661
56-57	0.0011466329400013954
58-59	0.003943300288476916
60-61	0.0015671518201621282
62-63	1.0025157895043375E-4
64-65	7.942726356319809E-4
66-67	3.3946841312833574E-4
68-69	1.0000832569311395E-4
70-71	0.0011639372592073026
72-73	0.0016218606150260738
74-75	5.459110683574093E-4
76-77	7.899690582997698E-4
78-79	9.775246447539672E-5
80-81	0.002439568675407145
82-83	0.0025082473427233097
84-85	0.004374907277227655
86-87	0.004302810115526061
88-89	0.07345166122496131
90-91	5.568503856831441E-4
92-93	3.137724256290321E-5
94-95	5.812893799334514E-5
96-97	0.007796708056598325
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	425.0
20-21	1434.0
22-23	2751.0
24-25	5824.0
26-27	8606.0
28-29	15806.0
30-31	33929.0
32-33	48242.0
34-35	69578.0
36-37	103145.0
38-39	107302.0
40-41	112356.0
42-43	126250.0
44-45	145915.0
46-47	148070.0
48-49	138172.0
50-51	128806.0
52-53	120235.0
54-55	134404.0
56-57	143100.0
58-59	88286.0
60-61	152483.0
62-63	148339.0
64-65	156185.0
66-67	166146.0
68-69	161918.0
70-71	159119.0
72-73	153888.0
74-75	158456.0
76-77	157541.0
78-79	167580.0
80-81	169648.0
82-83	161721.0
84-85	165922.0
86-87	175333.0
88-89	188391.0
90-91	200292.0
92-93	216366.0
94-95	279813.0
96-97	349658.0
98-99	1064909.0
100-101	6109002.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.05982368668974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.96488995473439	76.52397677542152
2	6.898643410725862	11.735947843873715
3	1.7319634749427788	4.419615234312578
4	0.6470411604050063	2.201488280883239
5	0.2937126721621291	1.2491574054328605
6	0.15756111923316915	0.804127261311052
7	0.09127995097539202	0.543497957526757
8	0.05590245116707651	0.3804042111940249
9	0.03549705521431404	0.2717435932133619
>10	0.11996783913940036	1.6162506958303382
>50	0.002838007768658924	0.15860774824816823
>100	6.87056553212171E-4	0.08574740430973579
>500	1.5846978604844634E-5	0.009435588442663805
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.100218495293692E-6	0.0
4	0.0	2.4300655485881076E-5	0.0	8.100218495293692E-6	8.100218495293692E-6
5	0.0	4.050109247646846E-5	0.0	1.6200436990587383E-5	8.100218495293692E-6
6	0.0	4.050109247646846E-5	0.0	1.6200436990587383E-5	8.100218495293692E-6
7	0.0	4.050109247646846E-5	0.0	1.6200436990587383E-5	8.100218495293692E-6
8	0.0	4.050109247646846E-5	0.0	1.6200436990587383E-5	8.100218495293692E-6
9	0.0	1.5390415141058015E-4	0.0	3.2400873981174766E-5	8.100218495293692E-6
10-11	0.0	1.5390415141058015E-4	0.0	4.4551201724115304E-5	8.100218495293692E-6
12-13	0.0	1.5390415141058015E-4	0.0	5.670152946705584E-5	8.100218495293692E-6
14-15	0.0	1.8630502539175491E-4	0.0	5.670152946705584E-5	2.4300655485881076E-5
16-17	0.0	1.8630502539175491E-4	0.0	5.670152946705584E-5	4.050109247646846E-5
18-19	0.0	1.9845535313469547E-4	0.0	7.695207570529008E-5	5.670152946705584E-5
20-21	0.0	2.10605680877636E-4	0.0	1.2960349592469906E-4	5.670152946705584E-5
22-23	0.0	2.4300655485881076E-4	0.0	2.268061178682234E-4	6.0751638714702684E-5
24-25	0.0	2.794575380876324E-4	0.0	2.956579750782198E-4	6.480174796234953E-5
26-27	0.0	2.997080843258666E-4	0.0	5.224640929464431E-4	6.480174796234953E-5
28-29	0.0	3.15908521316454E-4	0.0	0.0016159935898110915	6.480174796234953E-5
30-31	0.0	4.009608155170378E-4	0.0	0.005131488416768554	6.480174796234953E-5
32-33	0.0	4.3741179874585935E-4	0.0	0.012486486810495226	8.100218495293693E-5
34-35	4.050109247646846E-6	5.062636559558558E-4	0.0	0.023612136913781112	8.910240344823061E-5
36-37	8.100218495293692E-6	5.670152946705585E-4	0.0	0.0398652253245879	8.910240344823061E-5
38-39	8.100218495293692E-6	5.994161686517332E-4	0.0	0.06980363288319338	9.315251269587746E-5
40-41	8.100218495293692E-6	6.237168241376142E-4	0.0	0.1296318466894326	9.72026219435243E-5
42-43	8.100218495293692E-6	6.31817042632908E-4	0.0	0.15576315155525006	1.0125273119117115E-4
44-45	8.100218495293692E-6	6.966187905952575E-4	0.0	0.195271967266045	1.05302840438818E-4
46-47	8.100218495293692E-6	7.533203200623134E-4	0.0	0.24160116694987732	1.0935294968646484E-4
48-49	8.100218495293692E-6	7.533203200623134E-4	0.0	0.27999215250832177	1.2150327742940538E-4
50-51	8.100218495293692E-6	7.978715217864286E-4	0.0	0.3171276041999957	1.2150327742940538E-4
52-53	8.100218495293692E-6	8.181220680246628E-4	0.0	0.36555881058335665	1.2150327742940538E-4
54-55	8.100218495293692E-6	8.221721772723097E-4	0.0	0.47065104534129704	1.2150327742940538E-4
56-57	8.100218495293692E-6	8.343225050152503E-4	0.0	0.5792992760186713	1.2150327742940538E-4
58-59	8.100218495293692E-6	8.343225050152503E-4	0.0	0.6708843964357094	1.2150327742940538E-4
60-61	8.100218495293692E-6	8.464728327581908E-4	0.0	1.199192797026507	1.2150327742940538E-4
62-63	8.100218495293692E-6	8.66723378996425E-4	0.0	1.212404253392331	1.2150327742940538E-4
64-65	8.100218495293692E-6	9.072244714728935E-4	0.0	1.2267537904567436	1.2150327742940538E-4
66-67	8.100218495293692E-6	9.153246899681872E-4	0.0	1.2380171442744496	1.2150327742940538E-4
68-69	8.100218495293692E-6	9.193747992158341E-4	0.0	1.2497098096724062	1.2150327742940538E-4
70-71	8.100218495293692E-6	9.274750177111278E-4	0.0	1.2609974641455979	1.2150327742940538E-4
72-73	8.100218495293692E-6	9.355752362064214E-4	0.0	1.2698874539441827	1.2960349592469906E-4
74-75	8.100218495293692E-6	9.598758916923025E-4	0.0	1.2788665461462156	1.2960349592469906E-4
76-77	8.100218495293692E-6	0.0010125273119117113	0.0	1.2872624226165876	1.2960349592469906E-4
78-79	8.100218495293692E-6	0.0010449281858928862	0.0	1.2946255212288096	1.2960349592469906E-4
80-81	8.100218495293692E-6	0.0010449281858928862	0.0	1.2993884497040424	1.336536051723459E-4
82-83	8.100218495293692E-6	0.0010449281858928862	0.0	1.3033454064389933	1.4580393291528646E-4
84-85	8.100218495293692E-6	0.0010449281858928862	0.0	1.3070593566190856	1.4580393291528646E-4
86-87	8.100218495293692E-6	0.0010449281858928862	0.0	1.3075615701657937	1.4580393291528646E-4
88-89	8.100218495293692E-6	0.0010611286228834737	0.0	1.307626371913756	1.4580393291528646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12540	0.0	39.270504	1
GTATCAA	20050	0.0	29.08777	1
TCAACGC	24055	0.0	23.68585	4
ATCAACG	24125	0.0	23.549646	3
CAACGCA	24410	0.0	23.341383	5
AACGCAG	25060	0.0	22.687237	6
TATCAAC	25760	0.0	22.387346	2
ACCGAGG	17805	0.0	21.382538	92-93
GTACATG	30385	0.0	20.96205	1
GACCGAG	17880	0.0	20.736961	90-91
AGACCGA	18120	0.0	20.332087	90-91
TACATGG	31190	0.0	20.094814	2
GAGACCG	18350	0.0	20.041317	88-89
CGAGACC	18715	0.0	19.737091	88-89
ACATGGG	31080	0.0	19.576115	3
ACGAGAC	19700	0.0	19.412334	86-87
ACGCAGA	29890	0.0	18.993921	7
GCCCACG	19580	0.0	18.70791	82-83
CACGAGA	20145	0.0	18.498367	86-87
CCCACGA	19750	0.0	18.481592	84-85
>>END_MODULE