FastQCFastQC Report
Fri 27 May 2016
SRR2139563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9663422
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95700.046.3311121
TATCTCG144900.045.5193794-95
CCGTCTT58450.034.510592-93
CTATCTC187750.032.78145694-95
TATGCCG57700.032.562688-89
GTATCAA162850.032.10061
TGCCGTC60350.031.9753390-91
GCCGTCT61600.030.485590-91
ATGCCGT61600.029.43168888-89
ATCTCGT110450.029.28749894-95
GCTATCT225950.029.2046694-95
CGCTATC197550.028.33447592-93
CGTATGC68450.028.1996786-87
CTCGTAT68350.027.9336384-85
TCGTATG69450.026.28657384-85
TCTCGTA69600.025.9150682-83
TCAACGC200050.025.589854
ATCAACG203300.025.1807633
CAACGCA204250.024.9822675
TATCAAC210800.024.5819382
Produced by FastQC (version 0.11.5)