##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9663422
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.661678130169623	31.0	31.0	34.0	30.0	34.0
2	31.888511647323277	31.0	31.0	34.0	30.0	34.0
3	31.90614939511076	31.0	31.0	34.0	30.0	34.0
4	35.64997451213452	37.0	35.0	37.0	33.0	37.0
5	35.472724258549405	37.0	35.0	37.0	33.0	37.0
6	35.4605402723797	37.0	35.0	37.0	33.0	37.0
7	35.31526626902975	37.0	35.0	37.0	32.0	37.0
8	35.293822209151166	37.0	35.0	37.0	32.0	37.0
9	36.82151571151503	39.0	37.0	39.0	32.0	39.0
10-11	36.74728864164268	39.0	35.0	39.0	32.0	39.0
12-13	36.67457263068921	39.0	35.0	39.0	32.0	39.0
14-15	37.753941823093314	40.0	37.0	41.0	32.0	41.0
16-17	37.78874973068547	40.0	37.0	41.0	32.0	41.0
18-19	37.81451255052299	40.0	37.0	41.0	32.0	41.0
20-21	37.78547019522638	40.0	37.0	41.0	32.0	41.0
22-23	37.71060183185757	39.0	37.0	41.0	32.0	41.0
24-25	37.61482811106531	39.0	36.5	41.0	32.0	41.0
26-27	37.43930034442212	39.0	36.0	41.0	32.0	41.0
28-29	37.29432522624255	39.0	36.0	41.0	31.0	41.0
30-31	37.149081747587566	39.0	36.0	40.0	31.0	41.0
32-33	37.3837379471332	39.0	36.0	41.0	31.5	41.0
34-35	37.387054733756116	39.0	36.0	41.0	31.0	41.0
36-37	37.274057448155034	39.0	36.0	41.0	31.0	41.0
38-39	37.159560278527124	39.0	36.0	41.0	31.0	41.0
40-41	37.1475579247621	39.0	36.0	41.0	31.0	41.0
42-43	37.07302250810376	39.0	36.0	40.5	31.0	41.0
44-45	37.044687788362	39.0	35.5	40.0	31.0	41.0
46-47	37.049304527658904	39.0	35.5	41.0	31.0	41.0
48-49	36.96483607199571	39.0	35.0	40.5	30.5	41.0
50-51	36.87577026919602	39.0	35.0	40.0	30.0	41.0
52-53	36.74267374418497	39.0	35.0	40.0	30.0	41.0
54-55	36.644033836638044	39.0	35.0	40.0	30.0	41.0
56-57	36.46770930215162	38.0	35.0	40.0	30.0	41.0
58-59	36.32735500519868	38.0	35.0	40.0	30.0	41.0
60-61	36.1624757603395	38.0	34.0	40.0	29.0	41.0
62-63	35.933881488568055	38.0	34.0	40.0	29.0	41.0
64-65	35.74817846052515	37.0	34.0	40.0	29.0	41.0
66-67	35.5009322373195	37.0	34.0	39.5	29.0	41.0
68-69	35.22274999396393	36.0	34.0	39.0	28.5	41.0
70-71	34.93480648868155	36.0	33.0	39.0	28.0	40.0
72-73	34.63438609198428	35.5	33.0	38.5	28.0	40.0
74-75	34.29194677297589	35.0	33.0	37.5	28.0	40.0
76-77	33.188384644138395	34.5	31.5	36.0	26.0	39.0
78-79	33.51059111977852	35.0	32.0	36.5	27.0	39.0
80-81	33.407803905966304	35.0	32.0	36.0	27.0	38.5
82-83	33.217450498269166	35.0	32.0	36.0	27.0	37.0
84-85	33.02042880733224	35.0	32.0	35.0	27.0	37.0
86-87	32.88014618992173	34.0	32.0	35.0	27.0	36.5
88-89	32.68874339743602	34.0	32.0	35.0	27.0	36.0
90-91	32.62567100994831	34.0	32.0	35.0	27.0	36.0
92-93	32.61592287586991	34.0	32.0	35.0	27.0	36.0
94-95	32.601417556766975	34.0	32.0	35.0	27.0	35.0
96-97	32.499422417138135	34.0	32.0	35.0	27.0	35.0
98-99	32.64072997879938	34.0	32.0	35.0	28.0	35.0
100-101	32.14834215913378	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	11.0
20	108.0
21	551.0
22	2753.0
23	9396.0
24	24226.0
25	49363.0
26	87074.0
27	134293.0
28	187925.0
29	243405.0
30	302279.0
31	365616.0
32	441703.0
33	540848.0
34	683545.0
35	886784.0
36	1229226.0
37	1771024.0
38	1870182.0
39	803736.0
40	29370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.82795349307936	21.655879252711927	14.144389016644416	25.371778237564293
2	14.80900151970887	22.57579528584963	39.13981476834142	23.47538842610008
3	17.310399980462407	26.69805789294931	31.1957192803957	24.795822846192582
4	12.326637499635222	18.343802019615826	38.32993115689246	30.9996293238565
5	12.737289130082491	38.16200927580313	36.16964052692721	12.931061067187171
6	30.543207157878445	38.37747125190228	18.10078251782857	12.978539072390713
7	26.23899691020427	32.59106349696826	23.6891651839276	17.480774408899872
8	24.71292260650523	36.81086265300222	21.22772864519422	17.248486095298333
9	25.646059956814472	15.687869162704473	21.096036166070363	37.570034714410696
10-11	24.198399904298913	26.665812586886922	29.424592033753676	19.711195475060492
12-13	24.65499393093147	24.348148821559658	29.383915682608645	21.612941564900225
14-15	21.81982738619922	25.8302390188486	26.56417674815402	25.785756846798165
16-17	21.709488626285804	28.71294454490345	27.45409959329107	22.123467235519676
18-19	21.729162053029064	28.143035873576327	28.839557173576946	21.288244899817663
20-21	22.392305508701156	27.422279389248356	28.626688453057582	21.558726648992906
22-23	22.152638852547522	27.380931187297342	28.485234962125855	21.981194998029284
24-25	21.958996512279903	27.523446448878108	28.558161935895853	21.959395102946132
26-27	21.98141945063995	27.600211592707446	28.49841890694986	21.91995004970274
28-29	21.97552944336655	27.594721743382074	28.533824745265907	21.89592406798547
30-31	22.138859390127053	27.5469286322778	28.4395181512178	21.874693826377346
32-33	21.91284928134298	27.702213418751313	28.387562483966043	21.997374815939665
34-35	22.093152054054464	27.66559498304656	28.432899894330134	21.80835306856885
36-37	22.06847540038571	27.61454939905173	28.356294839826248	21.960680360736312
38-39	21.96025118194024	27.710161161702672	28.414951682062107	21.914635974294978
40-41	22.06434305619507	27.465881897592975	28.41043422554103	22.059340820670926
42-43	22.070085689175727	27.57856853274601	28.243515803391674	22.10782997468659
44-45	21.98821823165354	27.62016878914229	28.115752492509895	22.27586048669427
46-47	22.047865509369203	27.570091446397026	27.941317526142562	22.440725518091206
48-49	21.959722923326222	27.818283007804062	27.969330288134326	22.25266378073539
50-51	21.945785484961203	27.88756912834697	27.870073935566797	22.296571451125025
52-53	21.989206184288992	27.754132484472215	27.55204723488127	22.70461409635752
54-55	21.9586931451999	27.441292850538808	27.799876248058336	22.80013775620296
56-57	21.99963504686429	27.095181518938162	28.10882568023782	22.796357753959732
58-59	21.753791092140077	26.824010615597448	28.337400112918697	23.084798179343775
60-61	21.85314252803924	26.55813875971715	28.691108830145467	22.897609882098145
62-63	21.557517508001915	27.2391533034536	28.710001979071407	22.493327209473076
64-65	20.980466339372505	27.550072540560944	28.473069335266565	22.99639178479999
66-67	21.10236483765929	28.361260606051857	27.782772469451945	22.75360208683691
68-69	21.221440703321797	28.77054017591721	27.163117347811294	22.8449017729497
70-71	21.214224889551996	28.299716968743628	27.138366800685088	23.347691341019292
72-73	21.116005658788005	27.715758957434428	27.06534153854229	24.102893845235275
74-75	21.743809244801266	27.220661984028943	27.16193562986435	23.87359314130544
76-77	22.28035305355452	26.988611478411762	27.042655076187653	23.688380391846064
78-79	22.509525134912614	26.84445106275881	26.129925054661868	24.5160987476667
80-81	22.40479898397334	27.398339098897516	26.022489920249875	24.17437199687927
82-83	22.312922475762115	27.584032391349822	26.149136135781387	23.953908997106677
84-85	22.28900324240164	27.66837263501227	26.047129811228025	23.99549431135807
86-87	22.777072178664778	28.069959361627454	25.958194601628342	23.19477385807942
88-89	23.010606812488778	27.593577096952444	26.442976893754235	22.952839196804543
90-91	22.552550626472335	27.65884249159604	26.8179001777966	22.97070670413503
92-93	22.31766784022201	26.90242247383844	26.714293538954024	24.065616146985526
94-95	22.055474472150152	26.862097183651024	27.660187329980428	23.422241014218393
96-97	21.450445217848934	27.294550366590986	28.041501511184247	23.213502904375833
98-99	21.739673224086662	27.164950470328407	28.530934180501855	22.564442125083076
100-101	25.84781676945644	34.79458214596902	29.609783486138223	9.74781759843632
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2322.0
1	2070.5
2	2567.5
3	3522.5
4	4695.5
5	7109.0
6	9126.0
7	10661.0
8	11727.0
9	11855.5
10	11214.0
11	10500.5
12	11670.5
13	15330.0
14	23182.5
15	33698.0
16	41990.0
17	46368.0
18	47731.0
19	47158.0
20	45741.0
21	44801.5
22	45419.0
23	49375.0
24	57243.5
25	68619.5
26	83240.0
27	99621.5
28	118913.5
29	140838.0
30	162059.5
31	183297.5
32	205618.5
33	227874.0
34	249073.5
35	270005.0
36	293094.5
37	314502.0
38	332704.5
39	350006.0
40	365398.5
41	378621.0
42	387779.0
43	396995.5
44	407422.0
45	416225.0
46	422614.5
47	424551.0
48	425123.5
49	422444.0
50	416092.0
51	405823.5
52	389545.0
53	369032.0
54	345635.0
55	320303.0
56	292794.5
57	263595.0
58	233845.5
59	200576.5
60	166609.5
61	136367.5
62	108990.0
63	85017.0
64	64046.5
65	48487.5
66	37008.5
67	28160.0
68	21665.5
69	16202.0
70	12010.5
71	8814.5
72	6242.5
73	4408.5
74	3060.0
75	2081.5
76	1430.0
77	950.0
78	641.5
79	445.0
80	319.5
81	237.0
82	185.5
83	134.5
84	93.5
85	67.5
86	51.5
87	40.5
88	26.5
89	18.0
90	14.0
91	10.5
92	8.5
93	6.5
94	3.0
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004004792505180877
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.019933311408734E-4
14-15	0.0
16-17	0.0
18-19	4.3462864397311843E-4
20-21	0.0
22-23	0.0013144822094148182
24-25	2.1741261798646308E-4
26-27	0.004691942391647721
28-29	3.5234957056877925E-4
30-31	0.0
32-33	5.20477402692846E-6
34-35	2.038642840960663E-4
36-37	0.0228399838936285
38-39	0.012052554231319563
40-41	0.014849682725479383
42-43	0.0015144914464379619
44-45	0.0025558101193296303
46-47	0.0
48-49	6.426551335962668E-4
50-51	5.654554554265884E-6
52-53	1.7114745928173747E-5
54-55	0.004103937533726736
56-57	0.008344198831777802
58-59	0.010701912933941767
60-61	0.005645388415409246
62-63	0.006376341493706212
64-65	7.110316862383076E-4
66-67	9.021108672605203E-5
68-69	0.0
70-71	6.829750342822422E-5
72-73	0.0
74-75	3.012913797266928E-4
76-77	7.16930956746317E-5
78-79	0.0
80-81	0.0018837459997404617
82-83	0.002021530537900397
84-85	0.00903016389747474
86-87	0.009854991150118115
88-89	0.04376865823639294
90-91	5.276717768581589E-4
92-93	5.477237816797349E-4
94-95	0.0
96-97	0.009965338024749163
98-99	1.8671031614384809E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	314.0
20-21	1087.0
22-23	1990.0
24-25	3972.0
26-27	4965.0
28-29	10274.0
30-31	25851.0
32-33	37529.0
34-35	55386.0
36-37	78403.0
38-39	55585.0
40-41	88205.0
42-43	97097.0
44-45	113632.0
46-47	114513.0
48-49	107024.0
50-51	99133.0
52-53	16552.0
54-55	16952.0
56-57	17172.0
58-59	17961.0
60-61	116229.0
62-63	115437.0
64-65	121951.0
66-67	130063.0
68-69	130627.0
70-71	128867.0
72-73	125312.0
74-75	128258.0
76-77	127599.0
78-79	136289.0
80-81	134989.0
82-83	129199.0
84-85	129856.0
86-87	135948.0
88-89	143903.0
90-91	153833.0
92-93	160064.0
94-95	191918.0
96-97	237942.0
98-99	860244.0
100-101	5161297.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.37250705478834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.57268987775059	78.22990295437168
2	6.668755804362325	11.519943155178913
3	1.5709720649346928	4.070663872843414
4	0.5654060162477028	1.9534214050869791
5	0.24390408099573807	1.0533303478248024
6	0.1289981282243789	0.6685135044068795
7	0.07401374149979194	0.44749266868894416
8	0.04458330322305161	0.30806173377270335
9	0.028219836061425443	0.2193676190370395
>10	0.09988863231591091	1.3432341753555626
>50	0.0020397979597518456	0.11755433578275801
>100	5.287164246068751E-4	0.0685142276503448
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.069660209395802E-5	0.0	0.0	1.034830104697901E-5
3	0.0	3.104490314093703E-5	0.0	0.0	1.034830104697901E-5
4	0.0	6.208980628187407E-5	0.0	0.0	2.069660209395802E-5
5	0.0	6.208980628187407E-5	0.0	0.0	2.069660209395802E-5
6	0.0	6.208980628187407E-5	0.0	0.0	4.139320418791604E-5
7	0.0	6.208980628187407E-5	0.0	0.0	4.139320418791604E-5
8	0.0	7.243810732885307E-5	0.0	0.0	4.139320418791604E-5
9	0.0	1.1383131151676911E-4	0.0	3.104490314093703E-5	5.174150523489505E-5
10-11	0.0	1.1383131151676911E-4	0.0	3.621905366442654E-5	5.174150523489505E-5
12-13	0.0	1.1900546204025862E-4	0.0	4.139320418791604E-5	5.691565575838456E-5
14-15	0.0	1.914435693691117E-4	0.0	5.174150523489505E-5	8.796055889932159E-5
16-17	0.0	2.069660209395802E-4	0.0	7.243810732885308E-5	1.1383131151676911E-4
18-19	0.0	2.5353337565098575E-4	0.0	1.1383131151676911E-4	1.2417961256374813E-4
20-21	0.0	3.052748808858808E-4	0.0	4.7602184816103444E-4	1.2935376308723762E-4
22-23	5.174150523489505E-6	3.2597148297983883E-4	0.0	8.951280405636844E-4	1.6557281675166414E-4
24-25	1.034830104697901E-5	3.777129882147339E-4	0.0	0.001924783994738096	2.069660209395802E-4
26-27	1.034830104697901E-5	4.139320418791604E-4	0.0	0.0054225097486170016	2.069660209395802E-4
28-29	1.034830104697901E-5	4.1910619240264995E-4	0.0	0.025989758079487786	2.069660209395802E-4
30-31	1.034830104697901E-5	4.449769450200974E-4	0.0	0.04402167265384871	2.069660209395802E-4
32-33	1.034830104697901E-5	4.449769450200974E-4	0.0	0.06583589126088046	2.1731432198655921E-4
34-35	1.034830104697901E-5	5.174150523489505E-4	0.0	0.09709293457328055	2.2766262303353823E-4
36-37	1.034830104697901E-5	5.950273102012931E-4	0.0	0.13831021764339796	2.2766262303353823E-4
38-39	1.034830104697901E-5	6.157239122952512E-4	0.0	0.2186078596174316	2.3283677355702772E-4
40-41	1.034830104697901E-5	6.829878691006146E-4	0.0	0.6261394772990354	2.4835922512749626E-4
42-43	1.034830104697901E-5	7.036844711945727E-4	0.0	0.69641478970907	2.4835922512749626E-4
44-45	1.034830104697901E-5	7.812967290469152E-4	0.0	0.8126210363161207	2.4835922512749626E-4
46-47	1.034830104697901E-5	8.433865353287893E-4	0.0	0.9381355797149291	2.6388167669796474E-4
48-49	1.034830104697901E-5	8.485606858522789E-4	0.0	1.042653420289417	2.8975242931541227E-4
50-51	1.034830104697901E-5	8.744314384697264E-4	0.0	1.140320685570805	2.8975242931541227E-4
52-53	1.034830104697901E-5	8.796055889932158E-4	0.0	1.437554936543183	3.052748808858808E-4
54-55	1.034830104697901E-5	8.796055889932158E-4	0.0	2.406828554108472	3.2597148297983883E-4
56-57	1.034830104697901E-5	8.796055889932158E-4	0.0	3.467374186908116	3.363197840268178E-4
58-59	1.034830104697901E-5	8.899538900401948E-4	0.0	4.55842143704373	3.466680850737968E-4
60-61	1.034830104697901E-5	9.158246426576424E-4	0.0	5.381970279265461	3.5184223559728636E-4
62-63	1.034830104697901E-5	9.468695457985794E-4	0.0	5.405212563416976	3.5184223559728636E-4
64-65	1.034830104697901E-5	0.001003785201556964	0.0	5.418893017401082	3.5184223559728636E-4
66-67	1.034830104697901E-5	0.001034830104697901	0.0	5.429096442233403	3.6219053664426535E-4
68-69	1.034830104697901E-5	0.0010917457604562855	0.0	5.437664835500302	3.828871387382234E-4
70-71	1.034830104697901E-5	0.0011331389646442014	0.0	5.446383279132382	4.035837408321814E-4
72-73	1.034830104697901E-5	0.0011434872656911808	0.0	5.4533942530917106	4.760218481610345E-4
74-75	1.5522451570468516E-5	0.0011641838677851385	0.0	5.459851592945025	4.760218481610345E-4
76-77	2.069660209395802E-5	0.0011952287709260756	0.0	5.465977787164837	4.81195998684524E-4
78-79	2.069660209395802E-5	0.0012004029214495652	0.0	5.4713330329566485	4.863701492080135E-4
80-81	2.069660209395802E-5	0.0012055770719730547	0.0	5.47404946198148	5.01892600778482E-4
82-83	2.069660209395802E-5	0.0012107512224965441	0.0	5.4760570323845945	5.32937503919419E-4
84-85	2.069660209395802E-5	0.0012107512224965441	0.0	5.478204304851842	5.381116544429085E-4
86-87	2.069660209395802E-5	0.0012107512224965441	0.0	5.4785147538832515	5.381116544429085E-4
88-89	2.069660209395802E-5	0.0012728410287784183	0.0	5.478545798786393	5.381116544429085E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9570	0.0	46.331112	1
TATCTCG	14490	0.0	45.51937	94-95
CCGTCTT	5845	0.0	34.5105	92-93
CTATCTC	18775	0.0	32.781456	94-95
TATGCCG	5770	0.0	32.5626	88-89
GTATCAA	16285	0.0	32.1006	1
TGCCGTC	6035	0.0	31.97533	90-91
GCCGTCT	6160	0.0	30.4855	90-91
ATGCCGT	6160	0.0	29.431688	88-89
ATCTCGT	11045	0.0	29.287498	94-95
GCTATCT	22595	0.0	29.20466	94-95
CGCTATC	19755	0.0	28.334475	92-93
CGTATGC	6845	0.0	28.19967	86-87
CTCGTAT	6835	0.0	27.93363	84-85
TCGTATG	6945	0.0	26.286573	84-85
TCTCGTA	6960	0.0	25.91506	82-83
TCAACGC	20005	0.0	25.58985	4
ATCAACG	20330	0.0	25.180763	3
CAACGCA	20425	0.0	24.982267	5
TATCAAC	21080	0.0	24.581938	2
>>END_MODULE