FastQCFastQC Report
Fri 27 May 2016
SRR2139562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10241459
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103900.045.433881
GTATCAA175600.031.3591161
TCAACGC214900.024.918174
ATCAACG218250.024.5169373
CAACGCA221200.024.1159385
AACGCAG228550.023.3941236
TATCAAC231500.023.3447382
GTACATG267950.021.192821
TACATGG273600.020.4409312
ACATGGG275800.019.8167253
ACGCAGA276850.019.2980297
CGCAGAG286050.018.7059868
CATGGGG201100.016.9174084
AGAGTAC293900.016.6531710-11
GTGGTAT55750.016.3766881
GAGTACT186800.016.02107812-13
TGGTATC52600.015.7228642
GCAGAGT334700.015.7178959
CAGAGTA323650.015.12240610-11
TATACCG7000.014.6213275
Produced by FastQC (version 0.11.5)