##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139562_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10241459
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.634440952211985	31.0	31.0	34.0	30.0	34.0
2	31.85647103601157	31.0	31.0	34.0	30.0	34.0
3	31.869581668002578	31.0	31.0	34.0	30.0	34.0
4	35.621551870685614	37.0	35.0	37.0	33.0	37.0
5	35.43914289946384	37.0	35.0	37.0	33.0	37.0
6	35.424879013820195	37.0	35.0	37.0	33.0	37.0
7	35.282132653169825	37.0	35.0	37.0	32.0	37.0
8	35.263576215068575	37.0	35.0	37.0	32.0	37.0
9	36.78256984673766	39.0	37.0	39.0	32.0	39.0
10-11	36.70498314742069	39.0	35.0	39.0	32.0	39.0
12-13	36.63300800208252	39.0	35.0	39.0	32.0	39.0
14-15	37.69817034858022	39.0	37.0	41.0	32.0	41.0
16-17	37.73560666502692	39.0	37.0	41.0	32.0	41.0
18-19	37.762571719517695	39.5	37.0	41.0	32.0	41.0
20-21	37.734801393101776	39.5	37.0	41.0	32.0	41.0
22-23	37.66091921611597	39.0	37.0	41.0	32.0	41.0
24-25	37.563262304398805	39.0	36.0	41.0	32.0	41.0
26-27	37.38420069233755	39.0	36.0	41.0	31.5	41.0
28-29	37.23930597154285	39.0	36.0	40.5	31.0	41.0
30-31	37.09261091182228	39.0	36.0	40.0	31.0	41.0
32-33	37.324302805337894	39.0	36.0	40.5	31.0	41.0
34-35	37.32654992071777	39.0	36.0	41.0	31.0	41.0
36-37	37.21223789418562	39.0	36.0	41.0	31.0	41.0
38-39	37.09721883504241	39.0	36.0	41.0	31.0	41.0
40-41	37.083794286035925	39.0	36.0	40.5	31.0	41.0
42-43	37.010566150757796	39.0	35.0	40.0	31.0	41.0
44-45	36.98128540789696	39.0	35.0	40.0	30.5	41.0
46-47	36.98847617721031	39.0	35.0	40.0	30.5	41.0
48-49	36.90559414741393	39.0	35.0	40.0	30.0	41.0
50-51	36.816720377422705	39.0	35.0	40.0	30.0	41.0
52-53	36.68565269451197	39.0	35.0	40.0	30.0	41.0
54-55	36.5730548349035	38.5	35.0	40.0	30.0	41.0
56-57	36.388667201126864	38.0	35.0	40.0	30.0	41.0
58-59	36.237734113735115	38.0	34.5	40.0	29.0	41.0
60-61	36.06531070728319	38.0	34.0	40.0	29.0	41.0
62-63	35.82721395161635	37.5	34.0	40.0	29.0	41.0
64-65	35.61687036240946	37.0	34.0	40.0	29.0	41.0
66-67	35.36203619690581	37.0	34.0	39.5	28.0	41.0
68-69	35.0801572516082	36.0	33.5	39.0	28.0	40.5
70-71	34.79883519187939	36.0	33.0	39.0	28.0	40.0
72-73	34.49729324156082	35.0	33.0	38.5	28.0	40.0
74-75	34.18261029362094	35.0	33.0	37.5	28.0	39.5
76-77	33.12760503293977	34.5	31.5	36.0	26.0	39.0
78-79	33.50611907051454	35.0	32.0	36.0	27.0	39.0
80-81	33.441196137377005	35.0	32.0	36.0	28.0	38.5
82-83	33.26393796338923	35.0	32.0	36.0	27.5	37.0
84-85	33.05223981474458	35.0	32.0	35.0	27.0	37.0
86-87	32.897125822172825	34.0	32.0	35.0	27.0	36.5
88-89	32.71870035802843	34.0	32.0	35.0	27.0	36.0
90-91	32.6373169917676	34.0	32.0	35.0	27.0	36.0
92-93	32.61187777881561	34.0	32.0	35.0	27.0	36.0
94-95	32.61267845424922	34.0	32.0	35.0	27.0	35.0
96-97	32.534400415382954	34.0	32.0	35.0	27.0	35.0
98-99	32.68271259693822	34.0	32.0	35.0	28.5	35.0
100-101	32.214683415	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	3.0
19	21.0
20	125.0
21	807.0
22	3458.0
23	11232.0
24	27563.0
25	55974.0
26	96509.0
27	145856.0
28	200980.0
29	259753.0
30	321154.0
31	388617.0
32	469130.0
33	576004.0
34	728458.0
35	943060.0
36	1311045.0
37	1870146.0
38	1924985.0
39	878259.0
40	28315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.71976639265948	21.655918360850734	14.205759159900946	25.418556086588833
2	14.875781680099816	22.557108711755262	39.02396250708665	23.543147101058267
3	17.442221855304016	26.541784720321587	31.110127961260208	24.90586546311419
4	12.42602250323904	18.342630674008458	38.09189686742875	31.13944995532375
5	12.84846231381681	38.05709713821048	36.043800009354136	13.050640538618568
6	30.73812041819432	38.24503911015022	17.946427359617413	13.07041311203804
7	26.36599921944715	32.444908484230616	23.531344508629093	17.65774778769314
8	24.727648668026696	36.8806046091675	21.075649475333545	17.31609724747226
9	25.65048593174078	15.598451353464386	20.99526053856194	37.7558021762329
10-11	24.30328530339281	26.625605785269464	29.25283399562504	19.818274915712696
12-13	24.74645864254673	24.3238006141069	29.19033393606807	21.7394068072783
14-15	21.880720315337886	25.75711136469911	26.47153105822129	25.890637261741713
16-17	21.775998907968095	28.664387564310907	27.332946409295783	22.226667118425215
18-19	21.83965789883527	28.0736060397904	28.727187600541527	21.359548460832805
20-21	22.46137825921783	27.312149868910403	28.586242425162677	21.640229446709082
22-23	22.231782299271018	27.277356032971262	28.423040115569936	22.067821552187784
24-25	22.073912207389757	27.424399754977014	28.49650965328739	22.005178384345843
26-27	22.06002281564443	27.468480906558284	28.46473993840245	22.00675633939483
28-29	22.01866010685381	27.480323299474257	28.49004254516879	22.010974048503133
30-31	22.209761862465964	27.43064335150175	28.380646357917854	21.978948428114435
32-33	22.007869453302312	27.58033707147915	28.35702451580716	22.054768959411376
34-35	22.13255518127583	27.59329819203218	28.38663774533538	21.887508881356617
36-37	22.16836252987208	27.527181080573413	28.27499360437865	22.029462785175856
38-39	22.020145150842456	27.62720012665148	28.35122308843678	22.001431634069288
40-41	22.19808280267795	27.50240274163607	28.272208612223366	22.027305843462614
42-43	22.089158577699916	27.62770551747319	28.08486539891423	22.19827050591267
44-45	22.184291256408383	27.67619213652017	27.930969875585525	22.208546731485917
46-47	22.278169693367328	27.596737107193693	27.755820076853695	22.369273122585287
48-49	22.190183686023822	27.7546317962385	27.71467468955112	22.34050982818655
50-51	22.201509485836894	27.839044574431774	27.61781973232878	22.34162620740255
52-53	22.332929797375144	27.736642637689236	27.543402068322422	22.3870254966132
54-55	22.302958082681315	27.67372932163485	27.565508448448657	22.457804147235183
56-57	22.292633206571466	27.74834289295019	27.544688695435447	22.4143352050429
58-59	22.238545784282245	27.736650990118257	27.58658434385946	22.438218881740042
60-61	22.3124592953086	27.74933138932788	27.57309168928605	22.36511762607747
62-63	22.28762934271468	27.824748017499694	27.540843391457138	22.346779248328485
64-65	22.207747323259554	27.743317777451576	27.56129216038787	22.48764273890099
66-67	22.272315074052464	27.80515350950905	27.40839311983211	22.514138296606372
68-69	22.2129524461084	27.814013782260023	27.440509518937933	22.532524252693644
70-71	22.206607112662812	27.62152906241509	27.503860422727914	22.668003402194184
72-73	22.25584245848302	27.64638190634581	27.425485284754558	22.672290350416617
74-75	22.17587680050333	27.68707424140352	27.421903534234403	22.715145423858743
76-77	22.33085889849149	27.609268962488215	27.377270394757414	22.682601744262882
78-79	22.269593757853986	27.621744778217483	27.32219155333342	22.786469910595113
80-81	22.306569812591885	27.647713898878717	27.307845439730194	22.7378708487992
82-83	22.336004976189745	27.563788290819435	27.438726101864834	22.66148063112598
84-85	22.313591643932032	27.609985984054365	27.38840309365354	22.688019278360066
86-87	22.29398291642373	27.63783697017378	27.428816172858188	22.6393639405443
88-89	22.282264399474304	27.52685446985477	27.480025873437707	22.710855257233224
90-91	22.245138735645565	27.5733367042038	27.529202626962213	22.65232193318842
92-93	22.32555358495171	27.422025621208846	27.569987616981294	22.68243317685815
94-95	22.10207091474641	27.433113372720197	27.711853091348033	22.752962621185358
96-97	22.044748686783354	27.52027919053323	27.73489388983572	22.70007823284769
98-99	22.240969110902075	28.138531603686666	27.825447743341215	21.795051542070045
100-101	26.22721498743921	35.39940306119989	28.566077139151247	9.807304812209654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2650.0
1	2290.5
2	2774.0
3	3810.0
4	4978.5
5	7402.5
6	9602.0
7	11553.5
8	12950.0
9	13141.0
10	12402.5
11	11583.5
12	12651.5
13	16405.0
14	25194.0
15	37161.5
16	46235.5
17	50554.0
18	51697.0
19	51179.5
20	49710.0
21	48557.5
22	49316.5
23	54407.0
24	64179.0
25	78448.5
26	96018.5
27	114609.0
28	135587.5
29	158794.5
30	180331.5
31	201736.0
32	226131.5
33	250378.5
34	271834.0
35	293190.0
36	318086.5
37	339933.5
38	358048.0
39	375150.5
40	389243.5
41	402691.0
42	412834.5
43	421000.0
44	429643.0
45	438102.0
46	444776.5
47	446256.5
48	445267.5
49	443315.0
50	437585.5
51	428334.0
52	413225.5
53	391705.0
54	369120.5
55	345355.0
56	319031.5
57	289657.5
58	258421.5
59	224966.0
60	190643.0
61	157717.5
62	127093.0
63	99723.5
64	75461.0
65	57293.5
66	44005.0
67	33816.5
68	25867.5
69	19138.0
70	14185.5
71	10435.5
72	7439.0
73	5261.0
74	3589.5
75	2433.0
76	1670.5
77	1125.5
78	763.5
79	543.0
80	392.0
81	277.5
82	201.0
83	138.5
84	100.5
85	73.0
86	56.5
87	46.5
88	34.0
89	22.0
90	16.0
91	15.5
92	15.5
93	11.5
94	4.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0036811161378471563
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.055492874599216E-4
14-15	0.0
16-17	0.0
18-19	4.296262866452915E-4
20-21	0.0
22-23	0.0013770227829884363
24-25	2.4422134444729315E-4
26-27	0.004735116646348994
28-29	3.3247470111991167E-4
30-31	0.0
32-33	0.0
34-35	2.366876860124826E-4
36-37	0.022073977444949763
38-39	0.01117182207314897
40-41	0.01423905783656034
42-43	0.0017315012013562748
44-45	0.002811105307557317
46-47	5.213954356313611E-6
48-49	6.809924245135734E-4
50-51	2.670313389047464E-5
52-53	2.1592929870158472E-5
54-55	0.004256830288853768
56-57	0.008771142983770514
58-59	0.011202006003559363
60-61	0.005982127035905905
62-63	0.006880358710571428
64-65	8.487596859612415E-4
66-67	1.0024850424808933E-4
68-69	1.795952282745149E-5
70-71	1.398426563392257E-4
72-73	1.2348525271195225E-5
74-75	2.2568735906294607E-4
76-77	8.916644847424424E-5
78-79	1.295169103430197E-5
80-81	0.002089203589001325
82-83	0.00212658892921954
84-85	0.009291212260850272
86-87	0.010593472905351004
88-89	0.044064975177392184
90-91	3.9818972813849705E-4
92-93	6.668247782307494E-4
94-95	1.5198869994413656E-5
96-97	0.00986050870968458
98-99	1.8979744651466697E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	355.0
20-21	1177.0
22-23	2165.0
24-25	4389.0
26-27	5390.0
28-29	10501.0
30-31	26559.0
32-33	38325.0
34-35	56344.0
36-37	80481.0
38-39	84735.0
40-41	89279.0
42-43	101411.0
44-45	120027.0
46-47	120361.0
48-49	111688.0
50-51	102217.0
52-53	97689.0
54-55	107858.0
56-57	111838.0
58-59	113162.0
60-61	115286.0
62-63	110264.0
64-65	119517.0
66-67	126252.0
68-69	128496.0
70-71	127037.0
72-73	122121.0
74-75	125227.0
76-77	126524.0
78-79	134694.0
80-81	134360.0
82-83	129564.0
84-85	129418.0
86-87	136811.0
88-89	145260.0
90-91	156744.0
92-93	162883.0
94-95	196668.0
96-97	245797.0
98-99	798477.0
100-101	5384108.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.38256679041281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.40389331713132	79.87106812687995
2	6.1133897782014595	10.684073812190322
3	1.4062115660821979	3.68635128283886
4	0.5208887689566886	1.8206639057493499
5	0.21522909257134737	0.9403635278427851
6	0.11233721660864454	0.5889788600012372
7	0.06757442263282856	0.41333785493257075
8	0.0355655454027768	0.24862469172764828
9	0.023654508303071416	0.18602924865187612
>10	0.09835776259729677	1.3473664669963852
>50	0.002401221098898012	0.14408317722018102
>100	4.968004134582065E-4	0.06905904496887695
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.764233787392987E-6
2	0.0	2.9292701362178963E-5	0.0	0.0	9.764233787392987E-6
3	0.0	2.9292701362178963E-5	0.0	9.764233787392987E-6	9.764233787392987E-6
4	9.764233787392987E-6	4.882116893696494E-5	0.0	9.764233787392987E-6	9.764233787392987E-6
5	9.764233787392987E-6	5.8585402724357926E-5	0.0	9.764233787392987E-6	9.764233787392987E-6
6	3.905693514957195E-5	5.8585402724357926E-5	0.0	9.764233787392987E-6	2.9292701362178963E-5
7	3.905693514957195E-5	5.8585402724357926E-5	0.0	1.9528467574785974E-5	2.9292701362178963E-5
8	3.905693514957195E-5	7.81138702991439E-5	0.0	1.9528467574785974E-5	2.9292701362178963E-5
9	3.905693514957195E-5	1.4646350681089482E-4	0.0	2.9292701362178963E-5	3.905693514957195E-5
10-11	4.3939052043268445E-5	1.4646350681089482E-4	0.0	5.370328583066144E-5	4.882116893696494E-5
12-13	4.882116893696494E-5	1.4646350681089482E-4	0.0	5.8585402724357926E-5	4.882116893696494E-5
14-15	5.370328583066144E-5	2.392237277911282E-4	0.0	6.834963651175092E-5	5.8585402724357926E-5
16-17	1.1717080544871585E-4	2.4410584468482468E-4	0.0	6.834963651175092E-5	6.834963651175092E-5
18-19	1.1717080544871585E-4	2.6363431225961064E-4	0.0	7.811387029914391E-5	7.81138702991439E-5
20-21	1.1717080544871585E-4	2.8316277983439665E-4	0.0	2.0504890953525274E-4	8.29959871928404E-5
22-23	1.1717080544871585E-4	3.124554811965756E-4	0.0	4.979759231570423E-4	9.276022098023338E-5
24-25	1.2205292234241234E-4	3.417481825587546E-4	0.0	0.0013181715612980533	1.0740657166132286E-4
26-27	1.2693503923610883E-4	3.5151241634614756E-4	0.0	0.003920339865638284	1.1717080544871585E-4
28-29	1.2693503923610883E-4	3.5151241634614756E-4	0.0	0.02105168804561928	1.1717080544871585E-4
30-31	1.2693503923610883E-4	4.003335852831125E-4	0.0	0.03175817039349569	1.1717080544871585E-4
32-33	1.3181715612980532E-4	4.003335852831125E-4	0.0	0.03772899935448651	1.1717080544871585E-4
34-35	1.3669927302350184E-4	4.930938062633459E-4	0.0	0.046756033490931326	1.1717080544871585E-4
36-37	1.3669927302350184E-4	5.858540272435793E-4	0.0	0.059127317699558236	1.2205292234241234E-4
38-39	1.3669927302350184E-4	6.053824948183652E-4	0.0	0.08034011560266952	1.2693503923610883E-4
40-41	1.3669927302350184E-4	6.249109623931512E-4	0.0	0.1218381091990897	1.2693503923610883E-4
42-43	1.3669927302350184E-4	6.395573130742407E-4	0.0	0.13944302271775927	1.3181715612980532E-4
44-45	1.3669927302350184E-4	7.030248326922951E-4	0.0	0.16672917403662896	1.3669927302350184E-4
46-47	1.3669927302350184E-4	7.5184600162926E-4	0.0	0.19723752250533835	1.4646350681089482E-4
48-49	1.3669927302350184E-4	7.5184600162926E-4	0.0	0.22289304678171343	1.562277405982878E-4
50-51	1.4158138991719833E-4	7.90902936778832E-4	0.0	0.24703023270414887	1.562277405982878E-4
52-53	1.4646350681089482E-4	7.90902936778832E-4	0.0	0.27815372790146403	2.0016679264155623E-4
54-55	1.4646350681089482E-4	8.10431404353618E-4	0.0	0.34221686578054944	2.489879615785212E-4
56-57	1.4646350681089482E-4	8.250777550347075E-4	0.0	0.4075981752209329	2.5387007847221766E-4
58-59	1.4646350681089482E-4	8.397241057157969E-4	0.0	0.47668989350052565	2.5387007847221766E-4
60-61	1.562277405982878E-4	8.885452746527618E-4	0.0	0.519584172528543	2.5387007847221766E-4
62-63	1.659919743856808E-4	9.031916253338513E-4	0.0	0.5408360273668038	2.5387007847221766E-4
64-65	1.708740912793773E-4	9.861876125266918E-4	0.0	0.561585024165014	2.5387007847221766E-4
66-67	1.7575620817307378E-4	0.0010154803138888707	0.0	0.5720181079668434	2.5875219536591415E-4
68-69	1.7575620817307378E-4	0.0010154803138888707	0.0	0.5812404267790361	2.6363431225961064E-4
70-71	1.9040255885416325E-4	0.0010154803138888707	0.0	0.5896523141868751	2.6363431225961064E-4
72-73	2.1481314332264572E-4	0.0010154803138888707	0.0	0.5962968752791961	3.026912474091826E-4
74-75	2.1481314332264572E-4	0.0010545372490384426	0.0	0.6025801597213835	3.026912474091826E-4
76-77	2.2457737711003873E-4	0.0011033584179754076	0.0	0.6085168138641184	3.026912474091826E-4
78-79	2.2457737711003873E-4	0.0011228868855501935	0.0	0.6138773782133972	3.026912474091826E-4
80-81	2.2457737711003873E-4	0.0011228868855501935	0.0	0.6168603516354456	3.026912474091826E-4
82-83	2.2457737711003873E-4	0.0011228868855501935	0.0	0.6187643772239874	3.026912474091826E-4
84-85	2.2457737711003873E-4	0.0011228868855501935	0.0	0.6207074597476785	3.026912474091826E-4
86-87	2.2457737711003873E-4	0.0011424153531249795	0.0	0.6209759761768319	3.026912474091826E-4
88-89	2.2457737711003873E-4	0.0011619438206997654	0.0	0.621005268878194	3.026912474091826E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10390	0.0	45.43388	1
GTATCAA	17560	0.0	31.359116	1
TCAACGC	21490	0.0	24.91817	4
ATCAACG	21825	0.0	24.516937	3
CAACGCA	22120	0.0	24.115938	5
AACGCAG	22855	0.0	23.394123	6
TATCAAC	23150	0.0	23.344738	2
GTACATG	26795	0.0	21.19282	1
TACATGG	27360	0.0	20.440931	2
ACATGGG	27580	0.0	19.816725	3
ACGCAGA	27685	0.0	19.298029	7
CGCAGAG	28605	0.0	18.705986	8
CATGGGG	20110	0.0	16.917408	4
AGAGTAC	29390	0.0	16.65317	10-11
GTGGTAT	5575	0.0	16.376688	1
GAGTACT	18680	0.0	16.021078	12-13
TGGTATC	5260	0.0	15.722864	2
GCAGAGT	33470	0.0	15.717895	9
CAGAGTA	32365	0.0	15.122406	10-11
TATACCG	700	0.0	14.621327	5
>>END_MODULE