FastQCFastQC Report
Fri 27 May 2016
SRR2139561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6496718
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67500.041.8271561
GTATCAA110650.029.2186241
TCAACGC132300.023.5162664
CAACGCA134250.023.1746855
ATCAACG134800.023.1102243
AACGCAG138700.022.4896496
TATCAAC143400.022.121292
GTACATG168650.021.0702951
TACATGG169050.020.8044762
ACATGGG171100.020.1512373
ACGCAGA166600.018.7722157
CGCAGAG172850.018.0465038
CTTATAC47000.017.6933021
CATGGGG120800.017.4945834
AGAGTAC182500.015.88091510-11
GAGTACT115900.015.31195912-13
ATGGGGG69300.015.1600045
GTGGTAT36750.015.1222381
GCAGAGT204150.015.0809449
TATACAC69900.014.7397243
Produced by FastQC (version 0.11.5)