FastQCFastQC Report
Fri 27 May 2016
SRR2139560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11259279
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116050.043.666561
GTATCAA196400.030.5647561
TCAACGC235150.024.7148274
ATCAACG238800.024.3370653
CAACGCA241000.024.04745
AACGCAG249100.023.3307556
TATCAAC257000.023.104462
GTACATG281700.020.4722441
ACGCAGA294400.019.6860647
TACATGG287000.019.5840362
ACATGGG283950.019.3501243
CGCAGAG301550.019.2597548
CATGGGG214050.017.1000564
AGAGTAC319100.016.3587810-11
GCAGAGT356900.016.0221449
GAGTACT208850.015.84195612-13
CAGAGTA349700.015.22971310-11
ATGGGGG132250.014.6994975
GTGGTAT60050.014.5613471
TATACTG60550.014.3737455
Produced by FastQC (version 0.11.5)