##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139560_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11259279
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63586069765213	31.0	31.0	34.0	30.0	34.0
2	31.866541543201834	31.0	31.0	34.0	30.0	34.0
3	31.88177404610011	31.0	31.0	34.0	30.0	34.0
4	35.63158617883081	37.0	35.0	37.0	33.0	37.0
5	35.45114203138585	37.0	35.0	37.0	33.0	37.0
6	35.43625288972766	37.0	35.0	37.0	33.0	37.0
7	35.29119360129543	37.0	35.0	37.0	32.0	37.0
8	35.27007466463883	37.0	35.0	37.0	32.0	37.0
9	36.78720386980374	39.0	37.0	39.0	32.0	39.0
10-11	36.71461209905182	39.0	35.0	39.0	32.0	39.0
12-13	36.64063293928501	39.0	35.0	39.0	32.0	39.0
14-15	37.70840668394486	39.0	37.0	41.0	32.0	41.0
16-17	37.746920340103486	39.0	37.0	41.0	32.0	41.0
18-19	37.76989046101443	39.5	37.0	41.0	32.0	41.0
20-21	37.74055978187573	39.5	37.0	41.0	32.0	41.0
22-23	37.666732640128714	39.0	37.0	41.0	32.0	41.0
24-25	37.567857375298956	39.0	36.0	41.0	32.0	41.0
26-27	37.38932181735977	39.0	36.0	41.0	32.0	41.0
28-29	37.24365015704539	39.0	36.0	40.5	31.0	41.0
30-31	37.09541402345874	39.0	36.0	40.0	31.0	41.0
32-33	37.32731314741312	39.0	36.0	40.5	31.0	41.0
34-35	37.33410245480607	39.0	36.0	41.0	31.0	41.0
36-37	37.22068108341263	39.0	36.0	41.0	31.0	41.0
38-39	37.10876782886621	39.0	36.0	41.0	31.0	41.0
40-41	37.09674395878821	39.0	36.0	41.0	31.0	41.0
42-43	37.030498991601284	39.0	35.0	40.0	31.0	41.0
44-45	37.00410166158669	39.0	35.0	40.0	31.0	41.0
46-47	37.01037798000445	39.0	35.0	40.5	31.0	41.0
48-49	36.9248610657005	39.0	35.0	40.0	30.0	41.0
50-51	36.836023868592946	39.0	35.0	40.0	30.0	41.0
52-53	36.706053123201876	39.0	35.0	40.0	30.0	41.0
54-55	36.59808085010981	38.5	35.0	40.0	30.0	41.0
56-57	36.42002206611322	38.0	35.0	40.0	30.0	41.0
58-59	36.27135031035917	38.0	34.5	40.0	29.5	41.0
60-61	36.10194074748519	38.0	34.0	40.0	29.0	41.0
62-63	35.866375451176175	37.5	34.0	40.0	29.0	41.0
64-65	35.65450234683881	37.0	34.0	40.0	29.0	41.0
66-67	35.39922905929335	37.0	34.0	39.5	28.5	41.0
68-69	35.1205885210054	36.0	34.0	39.0	28.0	41.0
70-71	34.83986576571182	36.0	33.0	39.0	28.0	40.0
72-73	34.541750488817954	35.0	33.0	38.5	28.0	40.0
74-75	34.22440964514032	35.0	33.0	37.5	28.0	40.0
76-77	33.170559483107546	34.5	31.5	36.0	26.5	39.0
78-79	33.55085610141873	35.0	32.0	36.0	27.5	39.0
80-81	33.487241080137395	35.0	32.0	36.0	28.0	38.5
82-83	33.31149053901209	35.0	32.0	36.0	28.0	37.0
84-85	33.09517799376297	35.0	32.0	35.5	27.0	37.0
86-87	32.93911899870959	34.0	32.0	35.0	27.0	36.5
88-89	32.76202526005817	34.0	32.0	35.0	27.0	36.0
90-91	32.675694538904466	34.0	32.0	35.0	27.0	36.0
92-93	32.64194529068744	34.0	32.0	35.0	27.0	36.0
94-95	32.63788623586639	34.0	32.0	35.0	27.0	35.0
96-97	32.55689378657052	34.0	32.0	35.0	27.0	35.0
98-99	32.6991174687861	34.0	32.0	35.0	29.0	35.0
100-101	32.23801061057921	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	6.0
19	24.0
20	153.0
21	825.0
22	3490.0
23	11474.0
24	28869.0
25	58721.0
26	101759.0
27	156101.0
28	217422.0
29	282434.0
30	351311.0
31	426838.0
32	516947.0
33	633082.0
34	802283.0
35	1036836.0
36	1435750.0
37	2045979.0
38	2112820.0
39	1002046.0
40	34106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.563526136975554	21.851638990382956	14.222722431871526	25.362112440769963
2	14.77214076717776	22.72992315503562	39.038359243347244	23.45957683443938
3	17.252028304831953	26.856781859655488	31.2909556642126	24.600234171299956
4	12.403387463797637	18.565664817436357	38.26933323172825	30.761614487037757
5	12.817605816500327	38.100290436003945	36.08786139858511	12.994242348910618
6	30.382469428104585	38.42595960185373	18.134553731193623	13.057017238848065
7	26.02165733702842	32.61826090285177	23.78813954250534	17.571942217614467
8	24.640236732742835	36.76597764386156	21.29964094503742	17.29414467835818
9	25.599729787315866	15.673268243907982	21.151993835484493	37.57500813329166
10-11	24.218358031628846	26.619288854996842	29.401189898571655	19.76116321480265
12-13	24.653027419859804	24.35309425424154	29.32816569597684	21.665712629921817
14-15	21.860453942033057	25.757013393131125	26.596059125988443	25.786473538847382
16-17	21.73081864300547	28.67700054328523	27.41722183098936	22.174958982719943
18-19	21.770532288804155	28.101543839277543	28.81997627636441	21.307947595553888
20-21	22.39371611770333	27.36559772512589	28.628769270191928	21.61191688697885
22-23	22.161301774921903	27.34427165316821	28.480783631171647	22.013642940738233
24-25	22.030171169233547	27.464635393089726	28.56216786432068	21.943025573356042
26-27	22.02874411999053	27.546726141858112	28.48685644942442	21.937673288726938
28-29	21.987729061456157	27.591454104571607	28.48557672525961	21.935240108712627
30-31	22.15918702580942	27.53632634803837	28.395300881223996	21.909185744928212
32-33	21.962350491212757	27.648133849985634	28.390162062518492	21.999353596283118
34-35	22.08377641223335	27.649662684098864	28.388371605103103	21.87818929856468
36-37	22.0983935847292	27.611963594058324	28.336952692161933	21.952690129050534
38-39	22.012972774144206	27.68752932367173	28.368298754944693	21.931199147239376
40-41	22.16262728231084	27.584246509094474	28.277743286014335	21.97538292258035
42-43	22.056347182743032	27.73933523130668	28.07182267484952	22.132494911100768
44-45	22.15027161077828	27.785394760402397	27.906201498704565	22.15813213011476
46-47	22.266424734450442	27.737396225234644	27.722393304402598	22.273785735912313
48-49	22.198664806813166	27.81788015868889	27.6913328788211	22.29212215567684
50-51	22.179850485284618	27.90213953372089	27.62870763006705	22.289302350927436
52-53	22.299401544260412	27.821548200197064	27.545867150294406	22.33318310524811
54-55	22.275331070711385	27.763634307252783	27.560512336969666	22.400522285066167
56-57	22.293037141092164	27.874949387147403	27.487139647891638	22.344873823868795
58-59	22.236165114123942	27.882501913893154	27.534064934342855	22.347268037640053
60-61	22.356432652102814	27.87769794482325	27.483840353568755	22.28202904950518
62-63	22.350248503684288	27.981463076931856	27.423547528244725	22.244740891139138
64-65	22.29833881259583	27.868149526953285	27.455526913110727	22.377984747340157
66-67	22.32185770453747	27.88760663736854	27.362568711498085	22.427966946595905
68-69	22.27348394978332	27.833350292903376	27.42427110032917	22.468894656984133
70-71	22.247772934088818	27.646800206980416	27.532526495929154	22.57290036300161
72-73	22.353141406407563	27.725032145493838	27.387738307846604	22.534088140252
74-75	22.220284223314263	27.778409076093148	27.44890612355825	22.552400577034344
76-77	22.32816384672805	27.712398951569618	27.39501191556129	22.564425286141045
78-79	22.243849440287878	27.681732596625757	27.418263226932332	22.656154736154033
80-81	22.308495469673105	27.672231412149944	27.412500440105635	22.606772678071316
82-83	22.32194080975202	27.646832183268046	27.506885477359955	22.52434152961998
84-85	22.357563584651526	27.661115729615155	27.431579101897054	22.54974158383627
86-87	22.287483671385562	27.64432651323432	27.498153904355288	22.57003591102483
88-89	22.29766011522265	27.5800131160036	27.484034815376816	22.638291953396934
90-91	22.302858892071605	27.637982206217675	27.52350929448792	22.535649607222805
92-93	22.360134972358768	27.512567214201976	27.563178064108502	22.564119749330757
94-95	22.14747117519267	27.50886451544684	27.70061460969838	22.643049699662107
96-97	22.103633088736412	27.559485376612535	27.746578228690627	22.590303305960425
98-99	22.29007206464203	28.168943373839355	27.81295078725371	21.728033774264905
100-101	26.210355034293993	35.41071229650377	28.56710203854294	9.811830630659305
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2852.0
1	2593.0
2	3346.0
3	4631.5
4	6032.5
5	8911.0
6	11406.5
7	13553.5
8	15009.0
9	14922.0
10	13959.5
11	13055.0
12	14419.5
13	18966.5
14	29368.0
15	43461.5
16	54087.0
17	58708.0
18	59462.0
19	58876.5
20	57509.0
21	56815.5
22	58641.0
23	64945.5
24	76362.0
25	92496.0
26	112712.5
27	135401.5
28	160513.5
29	186283.5
30	209512.0
31	232472.5
32	257508.0
33	281247.0
34	303940.5
35	327028.0
36	352346.5
37	376424.5
38	396469.0
39	413870.0
40	428142.5
41	440794.5
42	451627.0
43	462258.0
44	472057.5
45	480511.0
46	486416.0
47	487805.5
48	489414.5
49	488498.5
50	480948.5
51	469814.0
52	453207.5
53	432062.5
54	407286.5
55	378576.5
56	350073.5
57	319523.5
58	286041.5
59	249677.5
60	210267.5
61	172960.0
62	139702.0
63	110091.5
64	83373.0
65	62542.0
66	47976.0
67	37032.0
68	28456.0
69	21302.0
70	15861.5
71	11651.5
72	8281.5
73	5756.5
74	3938.5
75	2701.0
76	1838.5
77	1210.0
78	836.5
79	605.0
80	425.5
81	289.0
82	217.5
83	164.0
84	119.5
85	87.0
86	64.0
87	44.5
88	30.0
89	21.0
90	14.5
91	11.5
92	10.0
93	6.5
94	3.0
95	2.5
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0033394678291567333
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.236825910433518E-4
14-15	0.0
16-17	0.0
18-19	3.8634800683063275E-4
20-21	0.0
22-23	0.0015101445514806278
24-25	1.9992576978307698E-4
26-27	0.005182696496537172
28-29	3.6920059264257057E-4
30-31	0.0
32-33	0.0
34-35	2.378539861949546E-4
36-37	0.021458114807734206
38-39	0.011126719299116924
40-41	0.014034917974595787
42-43	0.0017646902569221831
44-45	0.0029834314780492737
46-47	0.0
48-49	7.476532010144738E-4
50-51	1.4648920313535725E-5
52-53	2.4697728209107958E-5
54-55	0.004284857956958727
56-57	0.00929821765582052
58-59	0.012079525512538481
60-61	0.006232046716172531
62-63	0.0074432507597372004
64-65	9.105982657388206E-4
66-67	9.243904107436352E-5
68-69	0.0
70-71	1.0104262516823597E-4
72-73	0.0
74-75	1.970187699202636E-4
76-77	5.3011688252633445E-5
78-79	0.0
80-81	0.002184462746337074
82-83	0.0022992491398011834
84-85	0.00981327010997506
86-87	0.010733086573334152
88-89	0.04355189584585812
90-91	5.377441980425842E-4
92-93	7.087533098091458E-4
94-95	0.0
96-97	0.010324162679077228
98-99	6.89833366151517E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	392.0
20-21	1216.0
22-23	2392.0
24-25	4710.0
26-27	7293.0
28-29	13701.0
30-31	30176.0
32-33	43882.0
34-35	63566.0
36-37	92958.0
38-39	100992.0
40-41	103465.0
42-43	118621.0
44-45	139217.0
46-47	138419.0
48-49	128464.0
50-51	118576.0
52-53	118726.0
54-55	131037.0
56-57	136269.0
58-59	135871.0
60-61	132636.0
62-63	128904.0
64-65	136183.0
66-67	145015.0
68-69	143484.0
70-71	142492.0
72-73	137479.0
74-75	139706.0
76-77	141509.0
78-79	149941.0
80-81	149751.0
82-83	143956.0
84-85	144501.0
86-87	151674.0
88-89	161486.0
90-91	171187.0
92-93	177266.0
94-95	210772.0
96-97	265547.0
98-99	858933.0
100-101	5796914.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.63990850968774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.46203760904461	77.47160624438514
2	6.51825218265146	11.164450411306872
3	1.6823704327012405	4.322341498078141
4	0.6149349110417331	2.10651878084108
5	0.27109573634822876	1.1608307029114373
6	0.15031143421933207	0.772359448470213
7	0.09062310360208457	0.5432668010942534
8	0.05146844484745704	0.35262023262977044
9	0.035964352023593806	0.27719854352196993
>10	0.11994744466414511	1.60702397254537
>50	0.0024607162837919105	0.1422559994006679
>100	5.165171775662654E-4	0.07074745736682395
>500	1.7115394776688137E-5	0.00877990744826873
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.881563375416846E-6	0.0
2	0.0	1.776312675083369E-5	0.0	8.881563375416846E-6	0.0
3	0.0	1.776312675083369E-5	0.0	1.776312675083369E-5	0.0
4	0.0	5.328938025250107E-5	0.0	1.776312675083369E-5	0.0
5	0.0	5.328938025250107E-5	0.0	3.552625350166738E-5	0.0
6	1.5986814075750322E-4	5.328938025250107E-5	0.0	4.440781687708423E-5	8.881563375416846E-6
7	1.5986814075750322E-4	5.328938025250107E-5	0.0	4.440781687708423E-5	8.881563375416846E-6
8	1.5986814075750322E-4	6.217094362791792E-5	0.0	6.217094362791792E-5	8.881563375416846E-6
9	1.5986814075750322E-4	9.769719712958529E-5	0.0	7.105250700333477E-5	8.881563375416846E-6
10-11	1.6430892244521163E-4	9.769719712958529E-5	0.0	8.437485206646002E-5	8.881563375416846E-6
12-13	1.6874970413292007E-4	9.769719712958529E-5	0.0	9.769719712958529E-5	8.881563375416846E-6
14-15	1.6874970413292007E-4	1.1546032388041898E-4	0.0	1.0213797881729371E-4	3.99670351893758E-5
16-17	3.1973628151500645E-4	1.1546032388041898E-4	0.0	1.0657876050500213E-4	6.217094362791792E-5
18-19	3.1973628151500645E-4	1.4654579569437794E-4	0.0	1.3766423231896112E-4	7.105250700333477E-5
20-21	3.1973628151500645E-4	1.8207204919604532E-4	0.0	1.731904858206285E-4	7.549328869104319E-5
22-23	3.2417706320271485E-4	2.353614294485464E-4	0.0	2.442429928239632E-4	8.881563375416846E-5
24-25	3.2861784489042326E-4	2.664469012625053E-4	0.0	3.108547181395896E-4	9.325641544187687E-5
26-27	3.2861784489042326E-4	2.797692463256306E-4	0.0	4.396373870831339E-4	9.769719712958529E-5
28-29	3.2861784489042326E-4	2.8421002801333906E-4	0.0	0.0011546032388041899	9.769719712958529E-5
30-31	3.3305862657813173E-4	3.2861784489042326E-4	0.0	0.0027799293365054724	9.769719712958529E-5
32-33	3.46380971641257E-4	3.2861784489042326E-4	0.0	0.00602614075022033	9.769719712958529E-5
34-35	3.730256617675075E-4	3.8190722514292434E-4	0.0	0.010848829663071676	9.769719712958529E-5
36-37	3.8190722514292434E-4	4.351966053954254E-4	0.0	0.017359015617252224	9.769719712958529E-5
38-39	3.8190722514292434E-4	4.351966053954254E-4	0.0	0.026427091823552823	1.0657876050500213E-4
40-41	3.8190722514292434E-4	4.973675490233434E-4	0.0	0.035979213233813645	1.1546032388041898E-4
42-43	3.9522957020604957E-4	5.106898940864686E-4	0.0	0.044492191729150685	1.199011055681274E-4
44-45	4.2631504202000854E-4	5.773016194020948E-4	0.0	0.055150067779650896	1.2878266894354425E-4
46-47	4.4407816877084223E-4	6.30590999654596E-4	0.0	0.06626090356229737	1.3322345063125269E-4
48-49	4.4407816877084223E-4	6.30590999654596E-4	0.0	0.0763414779933955	1.4210501400666953E-4
50-51	4.973675490233434E-4	6.527949080931382E-4	0.0	0.08627550662879924	1.4210501400666953E-4
52-53	5.240122391495938E-4	6.572356897808465E-4	0.0	0.09531693814497358	1.7763126750833691E-4
54-55	5.240122391495938E-4	6.572356897808465E-4	0.0	0.10543303882957336	2.1315752101000427E-4
56-57	5.328938025250107E-4	6.705580348439718E-4	0.0	0.11586887579568816	2.1315752101000427E-4
58-59	5.328938025250107E-4	6.749988165316803E-4	0.0	0.1258428714662813	2.1315752101000427E-4
60-61	5.639792743389697E-4	6.794395982193887E-4	0.0	0.13460453373613	2.2203908438542112E-4
62-63	6.217094362791791E-4	6.927619432825139E-4	0.0	0.1429398809639587	2.3092064776083796E-4
64-65	6.30590999654596E-4	7.549328869104319E-4	0.0	0.1521678253110168	2.353614294485464E-4
66-67	6.439133447177212E-4	7.593736685981402E-4	0.0	0.1614446182566397	2.398022111362548E-4
68-69	6.483541264054297E-4	7.682552319735571E-4	0.0	0.1705215760263157	2.398022111362548E-4
70-71	6.572356897808465E-4	7.815775770366824E-4	0.0	0.17930100142291527	2.531245561993801E-4
72-73	7.549328869104319E-4	7.904591404120992E-4	0.0	0.18653947557388	2.930915913887559E-4
74-75	7.549328869104319E-4	8.082222671629329E-4	0.0	0.1931429179435024	2.930915913887559E-4
76-77	7.593736685981402E-4	8.481893023523087E-4	0.0	0.1994710318484869	2.9753237307646434E-4
78-79	7.638144502858487E-4	8.703932107908508E-4	0.0	0.2046756279864812	3.0197315476417275E-4
80-81	7.638144502858487E-4	8.703932107908508E-4	0.0	0.20764651093555814	3.0641393645188116E-4
82-83	7.682552319735571E-4	8.703932107908508E-4	0.0	0.20940061970220297	3.15295499827298E-4
84-85	7.860183587243908E-4	8.703932107908508E-4	0.0	0.21119025472234945	3.1973628151500645E-4
86-87	7.904591404120992E-4	8.703932107908508E-4	0.0	0.21150555022217676	3.1973628151500645E-4
88-89	8.082222671629329E-4	9.059194642925182E-4	0.0	0.21155883960242924	3.2861784489042326E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11605	0.0	43.66656	1
GTATCAA	19640	0.0	30.564756	1
TCAACGC	23515	0.0	24.714827	4
ATCAACG	23880	0.0	24.337065	3
CAACGCA	24100	0.0	24.0474	5
AACGCAG	24910	0.0	23.330755	6
TATCAAC	25700	0.0	23.10446	2
GTACATG	28170	0.0	20.472244	1
ACGCAGA	29440	0.0	19.686064	7
TACATGG	28700	0.0	19.584036	2
ACATGGG	28395	0.0	19.350124	3
CGCAGAG	30155	0.0	19.259754	8
CATGGGG	21405	0.0	17.100056	4
AGAGTAC	31910	0.0	16.35878	10-11
GCAGAGT	35690	0.0	16.022144	9
GAGTACT	20885	0.0	15.841956	12-13
CAGAGTA	34970	0.0	15.229713	10-11
ATGGGGG	13225	0.0	14.699497	5
GTGGTAT	6005	0.0	14.561347	1
TATACTG	6055	0.0	14.373745	5
>>END_MODULE